Applications Of Glufosinate Research Articles

D‐glufosinate as a male sterility agent for hybrid seed production

A chemical male sterility system based on anther-localized conversion of the inactive D-enantiomer of the herbicide, glufosinate (2-amino-4-(methylphosphinyl)-butanoate) to the phytotoxic L is described. Highly pure D-glufosinate was isolated in >98% enantiomeric excess from the racemate via fermentation with a strain of Escherichia coli expressing the PAT (L-glufosinate N-acetyl transferase) gene and purification of the unreacted D-enantiomer from the broth by ion exchange. A modified (F58K, M213S) form of the D-amino acid oxidase (DAAO) (EC 1.4.3.3) from Rhodosporidium toruloides was designed, tested in vitro and found to efficiently oxidize D-glufosinate to its 2-oxo derivative [2-oxo-4-(methylphosphinyl)-butanoic acid]. Tobacco (Nicotiana tabacum) plants were transformed to express this modified oxidase under control of the TAP1 tapetum-specific promoter. A number of the resultant transgenic lines exhibited complete male sterility that persisted for two or more weeks immediately following foliar treatment with 75 or 200 g/ha of D-glufosinate without exhibiting obvious phytotoxic symptoms or any measurable decline in female fertility. Similarly, plants containing the same construct and, additionally, a PAT gene expressed from a plastocyanin promoter exhibited significantly reduced male fertility and no reduction in female fertility following foliar application of racemic glufosinate. Thus, foliar application of d-glufosinate either purified or as the commercial herbicide, combined with anther expression of a modified DAAO promises to provide a cost-effective conditional chemical male sterility system with the characteristics necessary for practical F₁ hybrid seed production.

Can LibertyLink Cotton or Soybeans Work in Florida?

SS-AGR-339, a 3-page illustrated fact sheet by J. Ferrell, B. Brecke, and Clyde Smith, answers questions about the potential use of LibertyLink crops, which are genetically modified to tolerate applications of glufosinate, as part of a control strategy for Palmer amaranth control in cotton and soybeans. Published by the UF Department of Agronomy, January 2011.

Coupling native page/activity-staining with SDS-PAGE/immunodetection for the analysis of glutamine synthetase isoforms in spinach

Glutamine synthetase (GS) is a key nitrogen-assimilating enzyme in plants and a target for the broad-spectrum herbicide glufosinate. Understanding its kinetic and structural properties is of major agricultural importance. Spinach (Spinacia oleracea) is classified as a plant expressing only chloroplastic GS activity. We have analyzed soluble proteins in the spinach by coupling native polyacrylamide gel electrophoresis (PAGE)-activity detection, based on phosphate precipitation, with SDS-PAGE/immunoblotting. One cytosolic (GS1) isoform from the roots and two chloroplastic (GS2) isoforms expressed in leaves were resolved by native PAGE. The identity of the obtained bands was established by the application of GS-specific inhibitors, L-methionine sulfoximine and glufosinate. Examination by sodium dodecyl sulfate (SDS)-PAGE/ Western analysis with anti-GS antibodies, confirmed the identity of the active bands and revealed that both chloroplastic isoforms are composed of 44 kDa subunits, while the cytosolic isoform consists of 40 kDa subunits. The presence of more GS2 isozymes than encoded in the spinach genome is discussed in terms of posttranslational modifications.

Manejo de Conyza bonariensis com glyphosate + 2,4-D e amônio-glufosinate em função do estádio de desenvolvimento

Knowing the stage where the weed is more susceptible to chemical control is a fundamental part of weeds integrated management. In the specific case of C. bonariensis that is a weed with difficult control, this knowledge has a direct influence on herbicides efficiency. Thus the aim of the present study was to evaluate the effectiveness of glyphosate + 2,4-D and glufosinate herbicides in controlling C. bonariensis sprayed at different stages of development. Treatments were arranged in a 2x5 factorial scheme, where the first factor was represented by glyphosate + 2,4-D (960 + 496 g ha-1) and glufosinate (400 g ha-1) and second one for different development stages of C. bonariensis plants (cotyledon or 0, 7, 14, 21 and 28 cm height). For each development stage of this specie was used a check for evaluating the efficiency of these herbicides and dry mass comparison. The development stage of C. bonariensis plants at application time affects significantly the efficiency of glyphosate + 2,4-D and glufosinate herbicides. Excellent controls for this species occurred when glyphosate + 2,4-D and glufosinate applications were performed on plants taller than 6 cm. Satisfactory control of C. bonariensis was obtained when plants showed maximum height of 10 and 11 cm at application time of glyphosate+2,4-D and glufosinate herbicides, respectively.

Herbicide application increases nitrogen (15N) exudation and root detachment of Brachiaria decumbens Stapf

Current research shows that glyphosate herbicides can increase nitrogen exudation by plant roots and, as a consequence, favor pathogenic fungal colonization in soil. In Brazilian agroecosystems, the main herbicides used in conservationist systems for glyphosate substitution are glufosinate and paraquat. However, no studies have been developed that to evaluate the effects of these herbicides on nitrogen exudation by roots. This work was carried out with the objective of evaluating the exudation of nitrogen (15N) compounds and root detachment after the application of glyphosate, glufosinate-ammonium or paraquat on Brachiaria decumbens Stapf. The ammonium concentration in the plant’s tissues and the nitrogen (15N) recovery in the plant-solution system were also evaluated. The nitrogen left by the soil-solution system and the ammonium concentration in the plant’s tissues were increased after glyphosate or glufosinate application, but they were not modified by paraquat. Nitrogen compound exudation and root detachment were increased by the desiccation of Brachiaria decumbens Stapf by the use of the following herbicides: glyphosate, glufosinate-ammonium and paraquat. This increase demonstrated that the nitrogen exudation was not affected by a particular herbicide’s mode of action.

Weed Control and Crop Response to Glufosinate Applied to ‘PHY 485 WRF’ Cotton

Field experiments were conducted in Georgia to evaluate weed control and crop tolerance with glufosinate applied to ‘PHY 485 WRF®’ cotton. This glyphosate-resistant cotton also contains a gene, used as a selectable marker, for glufosinate resistance. Three experiments were maintained weed-free and focused on crop tolerance; a fourth experiment focused on control of pitted morningglory and glyphosate-resistant Palmer amaranth. In two experiments, PHY 485 WRF cotton was visibly injured 15 and 20% or less by glufosinate ammonium salt at 430 and 860 g ae/ha, respectively, applied POST two or three times. In a third experiment, glufosinate at 550 g/ha injured cotton up to 36%. Pyrithiobac or glyphosate mixed with glufosinate did not increase injury compared to glufosinate applied alone;S-metolachlor mixed with glufosinate increased injury by six to seven percentage points. Cotton injury was not detectable 14 to 21 d after glufosinate application, and cotton yields were not reduced by glufosinate or glufosinate mixtures. A program of pendimethalin PRE, glyphosate applied POST twice, and diuron plus MSMA POST-directed controlled glyphosate-resistant Palmer amaranth only 17% late in the season.S-metolachlor included with the initial glyphosate application did not increase control, and pyrithiobac increased late-season control by only 13 percentage points. Palmer amaranth was controlled 90% or more when glufosinate replaced glyphosate in the aforementioned system. Pitted morningglory was controlled 99% by all glufosinate programs and mixtures of glyphosate plus pyrithiobac. Seed cotton yields with glufosinate-based systems were at least 3.3 times greater than yields with glyphosate-based systems because of differences in control of glyphosate-resistant Palmer amaranth.

Weed Management in Glyphosate- and Glufosinate-Resistant Sugar Beet

Field experiments were conducted to determine the critical period of weed interference in glyphosate- and glufosinate-resistant sugar beet, and to determine if PRE herbicides increased weed control or sugar beet root yield when glufosinate, glyphosate, or conventional POST herbicides were applied. Glyphosate- and glufosinate-resistant sugar beet root yields were reduced by up to 66 and 67%, respectively, when weeds remained all season in the weedy control treatment compared with yields when weed removal occurred as soon as the weeds were 2.5 cm tall, approximately 2 to 3 wk after planting (WAP). A critical period of weed interference did not occur in this research. The critical time of weed removal was approximately 8 WAP in 1998 and beyond 11 WAP in 1999. Weeds averaged 20 cm in height at 8 WAP and weed densities were greater in 1998 compared with 1999. The critical weed-free period for glyphosate- and glufosinate-resistant sugar beet was 4.5 to 5 WAP in 1998. In 1999, the critical weed-free period at the Michigan Sugar location was 1.5 WAP in glyphosate-resistant sugar beet, and 6.5 WAP in glufosinate-resistant sugar beet for the Michigan Sugar site. Glyphosate or glufosinate POST provided better weed control and resulted in greater sugar beet root yield compared with conventional POST herbicides when data were combined over PRE herbicide treatments. PRE herbicides improved the control of common lambsquarters andAmaranthusspecies in some of the site-years when data were combined over POST treatments, but sugar beet yield did not increase. Our research suggests that PRE herbicides will not be necessary in glyphosate- or glufosinate-resistant sugar beet. To avoid sugar beet yield loss, multiple POST applications of glyphosate or glufosinate will be needed until 6 to 9 WAP to prohibit yield loss from weeds emerging after the last POST application.

Weed Control and Yield with Flumioxazin, Fomesafen, andS-Metolachlor Systems for Glufosinate-Resistant Cotton Residual Weed Management

Field studies were conducted near Clayton, Lewiston, and Rocky Mount, NC in 2005 to evaluate weed control and cotton response to preemergence treatments of pendimethalin alone or in a tank mixture with fomesafen, postemergence treatments of glufosinate applied alone or in a tank mixture withS-metolachlor, and POST-directed treatments of glufosinate in a tank mixture with flumioxazin or prometryn. Excellent weed control (> 91%) was observed where at least two applications were made in addition to glufosinate early postemergence (EPOST). A reduction in control of common lambsquarters (8%), goosegrass (20%), large crabgrass (18%), Palmer amaranth (13%), and pitted morningglory (9%) was observed when residual herbicides were not included in PRE or mid-POST programs. No differences in weed control or cotton lint yield were observed between POST-directed applications of glufosinate with flumioxazin compared to prometryn. Weed control programs containing three or more herbicide applications resulted in similar cotton lint yields at Clayton and Lewiston, and Rocky Mount showed the greatest variability with up to 590 kg/ha greater lint yield where fomesafen was included PRE compared to pendimethalin applied alone. Similarly, an increase in cotton lint yields of up to 200 kg/ha was observed whereS-metolachlor was included mid-POST when compared to glufosinate applied alone, showing the importance of residual herbicides to help maintain optimal yields. Including additional modes of action with residual activity preemergence and postemergence provides a longer period of weed control, which helps maintain cotton lint yields.

A Comparison of Weed Control in Herbicide-Resistant, Herbicide-Tolerant, and Conventional Corn

Experiments were conducted at three North Carolina research stations in 2003 to evaluate weed control and corn yield in glyphosate-resistant, glufosinate-resistant, imidazolinone-tolerant, and conventional corn weed management systems. Late-season control of common lambsquarters, large crabgrass, and yellow nutsedge increased with metolachlor PRE compared with no PRE herbicide treatment. Common lambsquarters, pitted morningglory, entireleaf morningglory, spurred anoda, and tropic croton control was improved by a single early POST (EPOST) application regardless of herbicide system. Control of common lambsquarters, pitted morningglory, entireleaf morningglory, and spurred anoda was similar for glyphosate and glufosinate systems for each POST over-the-top (POT) herbicide system. A single EPOST application of imazethapyr plus imazapyr to imidazolinone-tolerant corn controlled common lambsquarters, pitted morningglory, entireleaf morningglory, and spurred anoda and was better than a single EPOST application of glyphosate, glufosinate, or nicosulfuron. Tropic croton was controlled ≥ 95% with glufosinate or glyphosate, applied once or twice, or in mixture with metolachlor. A single EPOST application of imazethapyr plus imazapyr or nicosulfuron did not control tropic croton. Common lambsquarters, entireleaf morningglory, large crabgrass, Palmer amaranth, and yellow nutsedge control was greater with a late-POST–directed (LAYBY) of ametryn than no LAYBY. Systems that did not include a POT herbicide system had the lowest percentage in the weed-free yield and the lowest yield. Treatments that included a POT system with or without a PRE treatment of metolachlor yielded within 5% of the weed-free treatment, regardless of herbicide system.

Weed control in conventional and herbicide tolerant winter oilseed rape (Brassica napus) grown in rotations with winter cereals in the UK

SummaryTwo winter oilseed rape (Brassica napus) cultivars, tolerant to glyphosate and glufosinate, were compared with a conventional cultivar at three sites over 4 years, in 3‐year crop rotations in the UK. The winter oilseed rape was grown in Years 1 and 4, with winter cereals, which received uniform herbicide treatments, in the intervening years. The second winter oilseed rape treatments were applied to randomised sub‐plots of the original plots. Weed densities were recorded in autumn and spring and weed biomass was measured in summer. At most sites, there was only one application of glufosinate or glyphosate, whereas two products were often used on the conventional variety. The timing of glyphosate and glufosinate application was, on average, 34 days later than that of the conventional broad‐leaved weed control treatments. Overall weed control, across all sites and years, was not statistically different between the conventional, glyphosate and glufosinate treatments. However, glyphosate achieved higher control of individual weed species more frequently than the other treatments. Glufosinate and the conventional treatments were similar in performance. The treatments in Year 1 sometimes affected weed populations in the subsequent cereal crops and, in rare instances, those in the rape in Year 4. Carry‐over effects were small after most treatments. In general, weed survival was greater in the oilseed rape crops, irrespective of the treatment, than it was in the intervening cereal crops.

Glufosinate and ammonium sulfate inhibit atrazine degradation in adapted soils

The co-application of glufosinate with nitrogen fertilizers may alter atrazine cometabolism, thereby extending the herbicide’s residual weed control in adapted soils. The objective of this study was to assess the effects of glufosinate, ammonium sulfate, and the combination of glufosinate and ammonium sulfate on atrazine mineralization in a Dundee silt loam exhibiting enhanced atrazine degradation. Application of glufosinate at rates of 10 to 40 mg kg−1 soil extended the lag phase 1 to 2 days and reduced the maximum degradation rate by 15% to 30%. However, cumulative atrazine mineralization averaged 85% 21 days after treatment and was independent of treatment. Maximum daily rates of atrazine mineralization were reduced from 41% to 55% by application of 1 to 8 g kg−1 of ammonium sulfate. Similarly, cumulative atrazine mineralization was inversely correlated with ammonium sulfate rates ranging from 1.0 to 8 g kg−1 soil. Under the conditions of this laboratory study, atrazine degradation was relatively insensitive to exogenous mineral nitrogen, in that 8 g (NH4)2SO4 per kilogram soil repressed but did not completely inhibit atrazine mineralization. Moreover, an additive effect on reducing atrazine mineralization was observed when glufosinate was co-applied with ammonium sulfate. In addition, ammonium fertilization alters the partitioning of 14C-atrazine metabolite accumulation and nonextractable residues, indicating that ammonium represses cleavage of the triazine ring. Consequently, results indicate that the co-application of glufosinate with N may increase atrazine persistence under field conditions thereby extending atrazine residual weed control in adapted soils.

Physiological characteristics of glufosinate resistance in rice

The physiological basis of glufosinate resistance for two resistant (R) rice mutants, lines ‘R11-2’ and ‘R11-3’, was studied. Seven days after the application of 0.54 mM glufosinate, two susceptible (S) lines, i.e., variety (var.) ‘FSK’ and its inbred line ‘FSK-3’, and a reference var. Tainung 67 (TNG 67) suffered severe injury, whereas the two R lines exhibited resistance. Dose–response analysis and survival rate 14 d after treatment with 1.5 mM glufosinate also supported this observation. A14C-glufosinate experiment showed that more labeled herbicide was absorbed by leaves of R11-2 than S lines 48 h after treatment (HAT), but the partitioning of absorbed glufosinate to each part of the shoot did not differ between R and S lines. Although a higher degradation of glufosinate in R line R11-2 was found as compared with the two S lines, i.e., 46% vs. 38 to 40%, the actual concentration of glufosinate in R line was still higher than that in S lines. Foliar application of glufosinate resulted in less inhibition of in vivo activity of glutamine synthetase (GS; EC 6.3.1.2) as well as a lower accumulation of ammonium 24 HAT in R line than in S lines. Further kinetic study of GS showed that cytosolic GS in line R11-2, with a higher enzyme-inhibition constant (Ki) value to glufosinate, was less sensitive to the toxic action of this herbicide. Therefore, a higher metabolism of, and more important, a lower susceptibility of, the target protein GS to this herbicide are suggested to contribute significantly to glufosinate resistance in these rice lines.

Glufosinate Antagonizes Clethodim Control of Goosegrass (Eleusine indica)

Because of a previously reported antagonism of clethodim activity by other herbicides, greenhouse experiments were conducted to determine goosegrass control with clethodim and glufosinate postemergence alone, in tank mixtures, and as sequential treatments. Herbicide treatments consisted of glufosinate at 0, 290, or 410 g ai/ha and clethodim at 0, 105, or 140 g ai/ha, each applied alone, in all possible combinations of the above application rates, or sequentially. Glufosinate at either rate alone controlled goosegrass at the two- to four-leaf growth stage <44%, and control was less for goosegrass at the one- to two- and four- to six-tiller growth stages. Clethodim controlled two- to four-leaf and one- to two-tiller goosegrass 91 and 99% at application rates of 105 and 140 g/ha, respectively, and controlled four- to six-tiller goosegrass 68 and 83% at application rates of 105 and 140 g ai/ha, respectively. All tank mixtures of glufosinate with clethodim reduced goosegrass control at least 52 percentage points when compared to the control with clethodim alone. Glufosinate at 290 or 410 g/ha when applied sequentially 7 or 14 d prior to clethodim reduced goosegrass control at least 50 percentage points compared to the control obtained with clethodim applied alone. Clethodim at rates of 105 or 140 g/ha when applied 7 or 14 d prior to glufosinate controlled goosegrass equivalent to the control obtained with each respective rate of clethodim applied alone at the two- to four-leaf and one- to two-tiller growth stage. Clethodim should be applied to goosegrass no larger than at the one- to two-tiller growth stage at least 7 d prior to glufosinate application or 14 d after a glufosinate application for effective goosegrass control.

Control of Selected Broadleaf Weeds with Glufosinate as Influenced by Insecticide Coapplication

Coapplication of herbicides and insecticides affords growers an opportunity to control multiple pests with one application, given that efficacy is not compromised. Glufosinate was applied at 470 g ai/ha both alone and in combination with the insecticides acephate, acetamiprid, bifenthrin, cyfluthrin, dicrotophos, emamectin benzoate, imidacloprid, indoxacarb, lambda-cyhalothrin, methoxyfenozide, spinosad, or thiamethoxam to determine coapplication effects on control of some of the more common and/or troublesome broadleaf weeds infesting cotton. Hemp sesbania, pitted morningglory, prickly sida, redroot pigweed, and sicklepod were treated at the three- to four- or the seven- to eight-leaf growth stage. When applied at the earlier application timing, glufosinate applied alone provided complete control at 14 d after treatment, and control was unaffected by coapplication with insecticides. When glufosinate application was delayed to the later application timing, visual weed control was unaffected by insecticide coapplication. Fresh-weight reduction from the herbicide applied to larger weeds was negatively impacted by addition of the insecticides dicrotophos and imidacloprid with respect to redroot pigweed and prickly sida, but only in one of two experiments. In most cases, delaying application of glufosinate to larger weeds resulted in reduced control compared to that from a three- to four-leaf application, with the extent of reduction varying by species. Results indicate that when applied according to the herbicide label (three- to four-leaf stage), glufosinate/ insecticide coapplications offer producers the ability to integrate pest management strategies and to limit application costs without sacrificing control of the broadleaf weeds evaluated.

Time of Day of Application Effect on Glyphosate and Glufosinate Efficacy

Concurrent with the development of glyphosate- and glufosinate-resistant crops, applied research was conducted to maximize the effectiveness of these two herbicides. The objectives of this study were to examine the influence of time of day of herbicide application, adjuvant, and rate of glyphosate and glufosinate on annual weed control. Time of herbicide application influenced annual weed control of both glyphosate and glufosinate. Greatest annual weed control was observed between 0900 and 1800 h, while less weed control was observed at 0600, 2100, and 2400 h. Additional adjuvant or an increased rate of glyphosate or glufosinate improved efficacy, but did not overcome the time-of-day effect.

Efeitos da dessecação de plantas de soja no potencial fisiológico e sanitário das sementes

O objetivo da pesquisa foi verificar a melhor época de aplicação de dessecantes, a fim de permitir a colheita de sementes de soja com a máxima qualidade fisiológica e sanitária. Assim, foi semeada a cultivar IAC-15 na área experimental da FE/UNESP - Campus de Ilha Solteira, localizada no município de Selvíria, MS. O delineamento experimental utilizado foi o em blocos casualizados, estando os tratamentos dispostos em esquema fatorial 3 x 3 e 4 x 4 de produtos e épocas de aplicação, nos anos agrícolas de 1996/97 e 1997/98 respectivamente. Os dessecantes utilizados no ano agrícola de 1996/97 foram o paraquat, diquat e mistura paraquat + diquat nas doses de 400, 300 e 200+150 g ha-1 respectivamente. Em 1997/98, foram utilizados os mesmos dessecantes, nas mesmas doses, acrescentando-se, nesse caso, mais um tratamento, ou seja, o produto glufosinato de amônio, na dose de 400 g ha-1. Como épocas, foram realizadas três aplicações em 1997 e quatro em 1998, todas em intervalos de cinco dias a partir do estádio R6. Nas condições ambientais em que se realizou a pesquisa, a melhor época de dessecação foi quando as plantas estavam com 80% a 90% de vagens com coloração amarela e marrom e teores de água nas sementes entre 45% e 60%. Com relação aos dessecantes, não se obteve, com qualquer dos produtos testados, potencial fisiológico e sanitário suficientes para a comercialização das sementes.

Response of glufosinate-resistant Bt sweet corn (Zea mays) cultivars to glufosinate and residual herbicides

Field studies were conducted in 1999 and 2000 at Simcoe and Exeter, Ontario to determine the effect of glufosinate, applied alone or in combination with s-metolachlor/atrazine, on weed control and crop safety on six glufosinate-resistant Bt sweet corn cultivars. A single application of glufosinate at 0.5 or 1.0 kg ha-1 gave 89% or better control of redroot pigweed, common ragweed, wild buckwheat and lady’s-thumb in 1999 and pale smartweed and redroot pigweed in 2000. Glufosinate applied alone gave reduced control of wild buckwheat and barnyard grass in 2000 and reduced control of crab grass in 1999 and 2000 at Simcoe. S-metolachlor/atrazine applied preemergence, followed by glufosinate postemergence, improved control of these species. In the untreated control, yield of all cultivars, except Bonus Bt at Exeter, was reduced at all locations. Yield of Cupola Bt and SS Jubilee Bt was reduced when glufosinate was applied alone at 0.5 kg ha-1. Yield of Cupola Bt, Empire Bt and SS Jubilee Bt was reduced with s-metolachlor/atrazine used alone. Maximum yields were obtained with a sequential treatment of s-metolachlor/atrazine followed by glufosinate. Glufosinate did not cause any injury, plant height reduction or delayed maturity of any glufosinate-resistant Bt sweet corn cultivars. Key words: Cultivar, sensitivity, herbicide injury, glufosinate, Zea mays

Preemergence and Postemergence Control of Japanese Stiltgrass (Microstegium vimineum)1

Preemergence (PRE) and postemergence (POST) herbicides registered for large crabgrass control were evaluated for control of Japanese stiltgrass, an invasive, nonnative C4 annual grass. Benefin plus oryzalin, dithiopyr, isoxaben plus trifluralin, oryzalin, oxadiazon, pendimethalin, prodiamine, or trifluralin applied PRE controlled Japanese stiltgrass 87% or greater 8 wk after treatment. Benefin plus trifluralin, metolachlor, or napropamide applied PRE were less effective (78, 39, and 59% control, respectively). Single POST applications of clethodim, fenoxaprop-P, fluazifop-P, or sethoxydim controlled Japanese stiltgrass 50 to 88%. These herbicides applied twice provided 82 to 99% control. Single POST applications of glufosinate controlled Japanese stiltgrass 82 to 85%, whereas two applications provided complete control. Single POST applications of glyphosate were just as effective as two applications in controlling Japanese stiltgrass. Dithiopyr, MSMA, and quinclorac applied POST were ineffective on Japanese stiltgrass. All PRE and POST herbicides tested were equally or more effective on Japanese stiltgrass than on large crabgrass, with the exception of metolachlor applied PRE and dithiopyr or quinclorac applied POST.Nomenclature: Benefin; clethodim; dithiopyr; fenoxaprop-P; fluazifop-P; glufosinate; glyphosate; isoxaben; metolachlor; MSMA; napropamide; oryzalin; oxadiazon; pendimethalin; prodiamine; quinclorac; sethoxydim; trifluralin; Japanese stiltgrass, Microstegium vimineum (Trin.) A. Camus #3 MCGVM; large crabgrass, Digitaria sanguinalis (L.) Scop. # DIGSA.Additional index words: Annual jewgrass, bamboograss, flexible sesagrass, invasive plant, Japanese grass, Mary's grass, Nepalese browntop.Abbreviations: DAT, days after treatment; POST, postemergence; PRE, preemergence; WAT, weeks after treatment.

Evaluation of the Potential Role of Glufosinate-Tolerant Rice in Integrated Pest Management Programs for Rice Water Weevil (Coleoptera: Curculionidae)

The impact of a herbicide-tolerant rice, Oryza sativa L., variety was assessed for its resistance to rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), and its place in current integrated pest management (IPM) programs. Greenhouse experiments were conducted to evaluate the resistance of a glufosinate-tolerant rice variety and its glufosinate-susceptible parent line Bengal to the rice water weevil in the presence and absence of glufosinate applications. The LC50 dose-response and behavioral effects of glufosinate on adult rice water weevils also were studied. Field studies investigated the impacts of glufosinate-tolerant rice on rice water weevil management in the presence and absence of glufosinate under early and delayed flood conditions. Greenhouse studies demonstrated that in the absence of glufosinate, oviposition was 30% higher on the glufosinate-tolerant rice line than on Bengal rice or on glufosinate-tolerant line treated with recommended rates of commercially formulated glufosinate. Applications of glufosinate to glufosinate-tolerant rice resulted in a 20% reduction in rice water weevil larval densities compared with nontreated glufosinate-tolerant rice. The LC50 of glufosinate against adult rice water weevil was nearly 2 times the concentration recommended for application to glufosinate-tolerant rice. There was no difference in the amount of leaf area consumed by adult rice water weevils on glufosinate-treated and nontreated foliage. The absence of direct toxicity of glufosinate to rice water weevil at recommended glufosinate use rates and lack of behavioral effects suggest that the reduction in rice water weevil densities observed after glufosinate applications resulted from herbicide-induced plant resistance. Field experiments showed that neither rice variety nor herbicide use affected larval densities; however, delaying flood and applying insecticide effectively reduced numbers of rice water weevil larvae.

Impact of Injury from Late Season Sequential Glufosinate Applications on Sugarbeet Yield

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