Herbicide Glufosinate Ammonium Research Articles

Cellular and Genomic Instability Induced by the Herbicide Glufosinate-Ammonium: An In Vitro and In Vivo Approach.

Glufosinate-ammonium (GLA), an organophosphate herbicide, is released at high concentrations in the environment, leading to concerns over its potential genotoxic effects. However, few articles are available in the literature reporting the possible cellular and nuclear effects of this compound. We assessed, by in vitro and in vivo micronucleus assays, the genotoxicity of GLA on cultured human lymphocytes and Lymnaea stagnalis hemocytes at six concentrations: 0.010 (the established acceptable daily intake value), 0.020, 0.050, 0.100, 0.200, and 0.500 µg/mL. In human lymphocytes, our results reveal a significant and concentration-dependent increase in micronuclei frequency at concentrations from 0.100 to 0.500 μg/mL, while in L. stagnalis hemocytes, significant differences were found at 0.200 and 0.500 μg/mL. A significant reduction in the proliferation index was observed at all tested concentrations, with the only exception of 0.010 μg/mL, indicating that the exposure to GLA could lead to increased cytotoxic effects. In L. stagnalis, a significant reduction in laid eggs and body growth was also observed at all concentrations. In conclusion, we provided evidence of the genomic and cellular damage induced by GLA on both cultured human lymphocytes and a model organism's hemocytes; in addition, we also demonstrated its effects on cell proliferation and reproductive health in L. stagnalis.

Developmental synapse pathology triggered by maternal exposure to the herbicide glufosinate ammonium

Environmental and genetic factors influence synapse formation. Numerous animal experiments have revealed that pesticides, including herbicides, can disturb normal intracellular signals, gene expression, and individual animal behaviors. However, the mechanism underlying the adverse outcomes of pesticide exposure remains elusive. Herein, we investigated the effect of maternal exposure to the herbicide glufosinate ammonium (GLA) on offspring neuronal synapse formation in vitro. Cultured cerebral cortical neurons prepared from mouse embryos with maternal GLA exposure demonstrated impaired synapse formation induced by synaptic organizer neuroligin 1 (NLGN1)–coated beads. Conversely, the direct administration of GLA to the neuronal cultures exhibited negligible effect on the NLGN1-induced synapse formation. The comparison of the transcriptomes of cultured neurons from embryos treated with maternal GLA or vehicle and a subsequent bioinformatics analysis of differentially expressed genes (DEGs) identified “nervous system development,” including “synapse,” as the top-ranking process for downregulated DEGs in the GLA group. In addition, we detected lower densities of parvalbumin (Pvalb)-positive neurons at the postnatal developmental stage in the medial prefrontal cortex (mPFC) of offspring born to GLA–exposed dams. These results suggest that maternal GLA exposure induces synapse pathology, with alterations in the expression of genes that regulate synaptic development via an indirect pathway distinct from the effect of direct GLA action on neurons.

Development and Validation of Spectrophotometric and Spectrofluorimetric Methods for Glufosinate-Ammonium Herbicide and Carbofuran Insecticide Contamination in Environmental Samples

Development and Validation of Spectrophotometric and Spectrofluorimetric Methods for Glufosinate-Ammonium Herbicide and Carbofuran Insecticide Contamination in Environmental Samples

The herbicides glyphosate and glufosinate and the cyanotoxin β-N-methylamino-l-alanine induce long-term motor disorders following postnatal exposure: the importance of prior asymptomatic maternal inflammatory sensitization

BackgroundPrenatal maternal immune activation (MIA) and/or perinatal exposure to various xenobiotics have been identified as risk factors for neurological disorders, including neurodegenerative diseases. Epidemiological data suggest an association between early multi-exposures to various insults and neuropathologies. The “multiple-hit hypothesis” assumes that prenatal inflammation makes the brain more susceptible to subsequent exposure to several kinds of neurotoxins. To explore this hypothesis and its pathological consequences, a behavioral longitudinal procedure was performed after prenatal sensitization and postnatal exposure to low doses of pollutants.MethodsMaternal exposure to an acute immune challenge (first hit) was induced by an asymptomatic lipopolysaccharide (LPS) dose (0.008 mg/kg) in mice. This sensitization was followed by exposing the offspring to environmental chemicals (second hit) postnatally, by the oral route. The chemicals used were low doses of the cyanotoxin β-N-methylamino-l-alanine (BMAA; 50 mg/kg), the herbicide glufosinate ammonium (GLA; 0.2 mg/kg) or the pesticide glyphosate (GLY; 5 mg/kg). After assessing maternal parameters, a longitudinal behavioral assessment was carried out on the offspring in order to evaluate motor and emotional abilities in adolescence and adulthood.ResultsWe showed that the low LPS immune challenge was an asymptomatic MIA. Even though a significant increase in systemic pro-inflammatory cytokines was detected in the dams, no maternal behavioral defects were observed. In addition, as shown by rotarod assays and open field tests, this prenatal LPS administration alone did not show any behavioral disruption in offspring. Interestingly, our data showed that offspring subjected to both MIA and post-natal BMAA or GLA exposure displayed motor and anxiety behavioral impairments during adolescence and adulthood. However, this synergistic effect was not observed in the GLY-exposed offspring.ConclusionThese data demonstrated that prenatal and asymptomatic immune sensitization represents a priming effect to subsequent exposure to low doses of pollutants. These double hits act in synergy to induce motor neuron disease-related phenotypes in offspring. Thus, our data strongly emphasize that multiple exposures for developmental neurotoxicity regulatory assessment must be considered. This work paves the way for future studies aiming at deciphering cellular pathways involved in these sensitization processes.

The effect of herbicide glufosinate ammonium 150 g/L dose on several weeds and potatoes (Solanum tuberosum L.) yield

In the last three years, potato production in Indonesia fluctuated every year. One of the factors that cause low productivity of potatoes is weed. The presence of weeds in potato planting areas can inhibit plant growth and affect potato yields. The aim of this study is to determine the effectiveness of herbicide glufosinate ammonium 150 g/L to control common weeds in potato plants. This research was conducted in a farmer field in Lebak Muncang Village, Ciwidey District, Bandung Regency, West Java Province. The experimental design used was a randomized block design with six treatments and four replications. Weed control using the herbicide glufosinate ammonium 150 g/L at a dose of 2.75– 4.50 L/ha was completely (100%) controlled Eleusine indica, Galinsoga parviflora, Amaranthus spinosus, Richardia brasiliensis , and total weeds for up to 6 weeks after application without causing symptoms of poisoning and could increase the number of potato tuber per plant and yield of potato per plot.

Glufosinate Ammonium-Loaded Halloysite Nanotubes for Slow-Release Weeding Polymer Mulch Films

Improving the performance and giving function to poly(butylene adipate-co-terephthalate) (PBAT)-based biodegradable mulch film have always been research hotspots. However, the problems of a translucent PBAT-based film, which decreases in performance over time and is easily damaged by weeds, need to be solved. In this study, 11.17 wt % of the commercial contact herbicide Glufosinate-ammonium (Glu) was loaded into the lumen of halloysite nanotubes (HNTs). The Glu-loaded HNTs (Glu@HNTs) and other treatment groups were further incorporated into the PBAT/poly(lactic acid) (PLA) composite films by melt blending and blow molding. Results demonstrated that Glu, HNTs, and Glu@HNTs dispersed uniformly in the composite films. Remarkably, while maintaining the original tensile strength of the film, the nominal strain of transverse fracture was increased by 19.6% to 51.2%, and the least water vapor transmission was reduced by 46.7% to 68.5%. Furthermore, the composite films added with Glu@HNTs can successfully kill green bristlegrass and show a good slow-release effect, which can avoid premature release of the herbicide before the weeds grow up. Overall, the combination of inorganic nanofiller-modified PBAT/PLA film and weeding has positive significance for reducing soil pollution and helping the long-term efficient use of biodegradable mulching.

Oxidative and Histological Effects of Herbicide Glufosinate-Ammonium and Cyanobacteria Extracted Anatoxin-a on Land Snails Monacha cartusiana

Oxidative and Histological Effects of Herbicide Glufosinate-Ammonium and Cyanobacteria Extracted Anatoxin-a on Land Snails Monacha cartusiana

Equivalence of grain and forage composition in corn hybrid (Zea mays L.) from genetically modified off-patent (event TC1507) and non-genetically modified conventional corn

Off-patent corn (event TC1507) contains genes coding for CRY1F and PAT proteins, which confer resistance to lepidopteran insects and tolerance to the herbicide glufosinate-ammonium. We employed the substantial equivalence approach to investigate the compositional safety of the corn off-patent event (TC1507). The levels of the nutritional contents of proximate analytes in grain and forage tissues of off-patent genotypes of transgenic maize plants and conventional corn genotypes were compared. The levels of the analytes evaluated in the transgenic plants were found to be within the ranges published in the literature for non-transgenic corn and were statistically indistinguishable from the conventional corn from which they are derived (elite corn lines), indicating substantial equivalence between the off-patent (event TC1507) and its conventional counterpart. These results constitute key evidence of the safety evaluation of the world’s first transgenic corn developed from technologies that are in the public domain.

Harvest Anticipation, Yield and Economic Feasibility of Wheat by Application of Non-Selective Herbicides

The present work aims to evaluate the possibility of anticipating the wheat harvest by the application of non-selective herbicides in the pre-harvest conditions, thereby providing productive and economic viability. In total, 13 treatments were executed, which includes the spraying of different herbicide combinations (glufosinate-ammonium, glyphosate, and paraquat) in the phenological stages (Z-83, Z-85, Z-87, Z-92), and treatments without application of herbicides. Further, the treatment step was followed by providing different harvest conditions such as (i) glufosinate-ammonium: 9.5 and 8.8 days, (ii) glyphosate: 11.2 and 10.9 days, and (iii) paraquat: 7.9 and 8.5, where the first numerical value corresponds to number of days for the year 2016 followed by 2017. A mean reduction in seed yield of 4.6 % (BRS Parrudo) and 25.4 % (TBIO Sinuelo) was observed upon application of herbicides in the first two phenological stages. Additionally, an economic loss reaching up to R$ 2512 ha-1, was demonstrated. Therefore, the application of the non-selective herbicides glufosinate-ammonium and paraquat in the pre-harvest results in wheat phytointoxication, however, promoting harvest anticipation in 4.8 and 5.3 days, respectively.

Does the glufosinate-ammonium herbicide have the potential to induce the hormesis effect in upland rice?

This study aimed to evaluate the effects of low doses of the herbicide glufosinate-ammonium in different application modes in the vegetative development of upland rice. The treatment consisted of a combination of five low doses (0; 15; 30; 60; and 100 g a.i. ha−1) of the herbicide glufosinate-ammonium and four application modes of the low doses: single between active tillering (AT) and floral differentiation (FD); single after FD; split in two (the first at the beginning of the AT and the second between AT and FD; split in three (the first at the beginning of the AT, the second between the AT and the FD and the third after the FD, with. There was no hormesis effect on rice crop due to low doses of glufosinate-ammonium. The vegetative development of rice plants was reduced by the application of low doses in all application modes with lower plant height, dry weight, number of panicles, and effective tiller.

Effects of Low Doses of Glufosinate-Ammonium on Upland Rice Agronomic Traits

The use of herbicides and growth regulators in agricultural crops to reduce vegetative growth is widely studied worldwide. This study aimed to evaluate the agronomic traits of upland rice subjected to low doses of glufosinate-ammonium applied at different development stages. The treatments consisted of five low doses (0; 15; 30; 60; and 100 g a.i. ha−1; a.i.: active ingredient) of glufosinate-ammonium herbicide: a single low dose between active tillering (AT) and floral differentiation (FD); a single low dose after FD; low dose divided into two applications, the first at the beginning of the AT and the second between AT and FD; low dose divided into three applications, the first at the beginning of the AT, the second between AT and FD and the third after the FD, with four replications. The agronomic traits of upland rice were negatively affected due to the application of low doses of glufosinate-ammonium, with reduction in grain yield, 1000-grain weight, number of panicles, full spikelets and spikelets per panicle. Glufosinate-ammonium has no potential to be used as hormesis effect in rice plants due to loss in grain yield.

Comparison of RF3 <i>B. juncea</i> to RF3 <i>B. napus</i>

RF3 Brassica napus L. (InVigorTM Canola) has been in commercial cultivation since 1997. RF3 B. napus was produced by Agrobacterium-mediated transformation to provide glufosniate ammonium herbicide tolerance to the crop. Canola quality (CQ) varieties of Brassica juncea L. (Czern & Coss), a close relative of B. napus, have been available in Canada for more than 20 years. Currently, BASF Agricultural Solutions is developing CQ RF3 B. juncea via conventional breeding with RF3 B. napus to provide similar herbicide tolerance in the B. juncea species. The insertion event RF3 contains the bar gene (origin Streptomyces hygroscopicus) coding for phosphinothricin acetyl transferase (PAT/bar) protein which confers tolerance to glufosinate-ammonium herbicides. RF3 also contains the barstar gene (origin Bacillus amyloliquefaciens), coding for the Barstar protein, which is an inhibitor of the Barnase protein. In the absence of Barnase, there is no impact of Barstar. Many safety studies have been conducted during the development of the canola quality lines of RF3 B. napus and RF3 B. juncea. This report seeks to compare the two species based on the scientific data generated during the safety assessments of RF3 B. napus and RF3 B. juncea and discusses the utility of using studies with one species to support the safety of the other species both containing the same insertion event.

Initial Serum Ammonia as a Predictor of Mortality in Patients with Acute Glufosinate Ammonium Herbicide Poisoning

Purpose: The association of the initial serum ammonia level with in-hospital mortality in patients with acute glufosinate-ammonium herbicide poisoning was studied. Methods: This retrospective cohort study was conducted between March 2012 and August 2019 in the emergency department after glufosinate-ammonium herbicide poisoning. Survivors and non-survivors were analyzed using a Mann-Whitney U test and Fisher's exact test. Multivariate logistic regression analysis was performed to determine the independent risk factors for mortality. Results: One hundred and six patients were enrolled; 11 died, yielding a mortality of 10.4%. The serum bicarbonate level was significantly lower in the non-survival group than the survival group. Age, serum ammonia, blood urea nitrogen, creatinine levels, SOFA score, and APACHE II score were significantly higher in the non-survival group than the survival group. Age, serum ammonia, and creatinine level were independent risk factors for mortality in multivariate logistic regression analysis. Conclusion: The initial serum ammonia level is associated with mortality in patients with acute glufosinate herbicide poisoning.

Transformation and molecular identification of maize phytochrome <italic>A1</italic> gene (<italic>ZmPHYA1</italic>) in cotton

<p indent=0mm>In order to evaluate the potential value of maize <italic>phytochrome A1</italic> gene (<italic>ZmPHYA1</italic>) in the improvement of cotton germplasm resources, we transferred it into upland cotton (<italic>Gossypium hirsutum </italic>L.) R15 <italic>via</italic> <italic>Agrobacterium tumefaciens</italic>-mediated transformation with glufosinate-resistance gene as selection marker. The regenerated cotton plants were obtained through callus induction, antibiotic resistance screening and differentiation induction. After screening the regenerated plants by the herbicide glufosinate ammonium in the field, PCR detection confirmed that both the target bands, including <sc>256 bp</sc> of the glufosinate gene and <sc>217 bp</sc> band of <italic>ZmPHYA1</italic> gene, were detected in the homozygous transgenic plants. In addition, the exogenous ZmPHYA1 protein of about 170 kD was also checked by immuno-blot in three transgenic cotton lines. The results showed that the specific proteins could be detected in different tissues, including leaves, flowers and stems in the transgenic Line 9. The plant height of transgenic Line 9, Line 14, Line 41 were significantly shorter than that of the wild type, while the differences of other yield-related agronomic traits were not observed between the transgenic lines and the wild type. In this study, new cotton germplasms with glufosinate resistance and <italic>ZmPHYA1</italic> gene were successfully obtained, which provided a material source for further utilization of phytochrome gene to innovate germplasm resources.

ARE GLYPHOSATE AND GLUFOSINATE-AMMONIUM TOTALLY SELECTIVE FOR TRANSGENIC MAIZE CONTAINING THE CP4-EPSPS AND PAT GENES?

required. Maize with cp4-epsps and pat genes is tolerant to glyphosate and glufosinate-ammonium herbicides and has been a tool used for weed control. However, there is a demand for new studies to assess how tolerant it is and to avoid yield losses. The objective of this study was to assess the selectivity of the 2B810PW hybrid, which has the cp4-epsps and pat genes, subjected to applications of glyphosate (gly) and glufosinate-ammonium (glu), both isolated and combined, at two different rates, during three crop years, in two municipalities in Paraná state. A randomized block design, with four replications, was used. Some measured variables, such as plant height, showed few differences between crop years and locations, while others, like percentage of seed germination and yield, showed no differences (P ≤ 0.05). This demonstrates that the hybrid is resistant to the application of glufosinate-ammonium and glyphosate herbicides, isolated or combined, and these molecules can provide more options for weed control in crops of maize containing the cp4-epsps and pat genes.

Exposure Impacts of Environmentally Relevant Concentrations of a Glufosinate Ammonium Herbicide Formulation on Larval Development and Thyroid Histology of Xenopus laevis.

Thyroid hormones play critical roles in body growth and development as well as reproduction. They also influence the activities of a wider variety of tissues and biological functions, such as osmoregulation, metabolism, and especially metamorphosis in organisms, such as frogs. These complex activities of thyroid hormones are prone to disruption by agricultural pesticides, often leading to modulation of growth and the reproductive system in particular. These substances include Glufosinate ammonium, Glyphosates, Imazapyr, Penoxsulam, and Diquat dibromide among other herbicides. In this study, the standardized Xenopus Metamorphosis Assay protocol was used to assess the potential thyroid-modulatory properties of the Glufosinate ammonium Basta formulation, at relevant environmental concentrations (0.05mg/L, 0.15mg/L, and 0.25mg/L) for 21days. The results showed that this formulation only reduced the hind-limb length among the morphological endpoints. Histological evaluation showed that the mean thyroid gland area and the mean thyroidal follicle epithelium height were significantly increased following 0.15 and 0.25mg/L exposures. The present study confirmed that this Basta formulation interacts with the thyroid axis and therefore potentially pose health hazard to amphibian in particular and potentially metamorphic aquatic vertebrates. Furthermore, the result is a signal of inherent potential thyroid disrupting activities that must be further investigated and characterised in some of the aquatic herbicide formulations to safeguard the aquatic biodiversity.

Food/Feed and Environmental Risk Assessment of Genetically Modified Glufosinate-tolerant Oilseed Rape MS8, RF3 and MS8 x RF3 for Import, Processing and Feed Uses under Directive 2001/18/EC (Notification C/BE/96/01)

Food/Feed and Environmental Risk Assessment of Genetically Modified Glufosinate-tolerant Oilseed Rape MS8, RF3 and MS8 x RF3 for Import, Processing and Feed Uses under Directive 2001/18/EC (Notification C/BE/96/01)

Environmental Risk Assessment of Insect-resistant and Herbicide-tolerant Genetically Modified Maize 1507 x 59122 for Food and Feed Uses, Import and Processing under Regulation (EC) No 1829/2003 (EFSA/GMO/NL/2005/15)

Environmental Risk Assessment of Insect-resistant and Herbicide-tolerant Genetically Modified Maize 1507 x 59122 for Food and Feed Uses, Import and Processing under Regulation (EC) No 1829/2003 (EFSA/GMO/NL/2005/15)

Food/Feed and Environmental Risk Assessment of Insect-resistant and Herbicide-tolerant Genetically Modified Maize 1507 x 59122 for Food and Feed Uses, Import and Processing under Regulation (EC) No 1829/2003 (EFSA/GMO/NL/2005/15)

Food/Feed and Environmental Risk Assessment of Insect-resistant and Herbicide-tolerant Genetically Modified Maize 1507 x 59122 for Food and Feed Uses, Import and Processing under Regulation (EC) No 1829/2003 (EFSA/GMO/NL/2005/15)

Environmental Risk Assessment of Glufosinate-Tolerant Genetically Modified Oilseed Rape MS8, RF3 and MS8 x RF3 for Import, Processing and Feed Uses under Directive 2001/18/EC (Notification C/BE/96/01)

Environmental Risk Assessment of Glufosinate-Tolerant Genetically Modified Oilseed Rape MS8, RF3 and MS8 x RF3 for Import, Processing and Feed Uses under Directive 2001/18/EC (Notification C/BE/96/01)