Event Abstract Back to Event Low-cost fabrication of a glucose microbiosensor Jehú López-Aparicio1, 2, Aarón Cruz-Ramírez1, 2, Mariana Centeno-Sierra1, 2, Mathieu Hautefeuille1, 2 and Catalina Stern1, 2 1 Universidad Nacional Autonoma de Mexico, Facultad de Ciencias, Mexico 2 Universidad Nacional Autonoma de Mexico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia, Facultad de Ciencias, Mexico Introduction: Frequent glucose monitoring is a necessity in regions where type 1 and type 2 diabetes are amongst the most important non infectious diseases, but its cost may limit diagnostics, prevention and treatment in developing countries like Mexico. There is thus an increasing need for cost-effective, point-of-care platforms enabled by rapid prototyping methods to fabricate conducting microelectrodes on cheap substrates. This would enable massive screenings and the construction of stronger region-specific databases, Here, we present the development of a low-cost, simple technique using a laser-based CD-DVD platform to etch microchannels and fill them with different conductive pastes in both rigid polymethylmethacrylate (PMMA) and soft polydimethylsiloxane (PDMS) to demonstrate its potential in plastics and flexible electronics biosensors, similar to commercial systems. Materials: Cured but flexible polydimethylsiloxane (PDMS) and rigid polymethylmethacrylate (PMMA) have been used in this work to test for different substrates and resolutions. Channels were filled with carbon and silver conductive pastes and for this different materials were tested: either as purchased or prepared using commercial or fabricated powders with mineral oils. Methods: We obtained microchannels at the surface of the materials of interest using a very low-cost direct laser system capable of etching custom-made 3D designs on transparent polymers[1]. Filling of the channels was achieved using a doctor-blading technique and possible remainings are washed using isopropyl alcohol and water. Conductive pastes are cured using low temperature convective heating to promote adhesion to the substrates and washed lightly at the very end of the process. Glucose oxidase was then used to functionalize carbon electrodes. Specialized embedded electronics was designed and fabricated for low currents signal conditioning and converting to readable voltages. Results: All conductive pastes were tested with LCR-meter under dry and humid conditions before and after funcionalization. The electrochemical embedded electronics platform was tested against a commercial one and the complete biosensor system was successfully validated by comparison with commercial glucose sensors; it responded with similar performance in all important aspects: speed, accuracy and repeatability. A final stage for telemedicine was implemented for smartphone compatibility to reduce collection time and construct patients timelines and databases if necessary. Discussion: The use of low-cost equipment and materials is currently aimed at the simple development of do-it-yourself kits for massification of this technology. More efforts are being made on reducing the overall cost using different materials, avoiding the functionalization with glucose oxidase by using ionomers membranes and testing the biosensor ageing and reusability. Conclusions: We report a low-cost platform for glucose monitoring in diabetes, capable of competing with existing glucometers. It was successfully tested against commercial systems and offered similar performance. thanks to our developed electronics,it was possible to convert low electrochemical currents obtained at the microelectrodes into signals readable by a microcontroller for real-time telemedicine applications. It is a very promising platform for point-of-care glucose monitoring with interconnection capability for patients database construction. CONACyT grants #224450, #252043 and #246988; PAPIIT grant #TA100315; Google Research Award for Latinamerica