Glucose Extract Research Articles

Extraction of Glucose by Using Alkaline Hydrolysis from Musa Sapientum Peels, Ananas Comosus and Mangifera Indica Linn

Agricultural country in East Asia has generated abundant plantations wastes that contain cellulose. It is then hydrolysed to glucose catalytically or through enzymatic approach. Glucose product is widely used in food industries. Alternatively, it can be converted into biofuel resources that reduce fossil fuel dependency. In this study, glucose was extracted from three different types of fruits peel wastes which are Musa Sapientum (banana), Ananas Comosus (pineapple) and Mangifera Indica Linn (mango); by using alkaline hydrolysis method and the concentration obtained were compared accordingly. The alkali is used to remove the lignin content in fruits peel. The process started with samples and solvent preparation, followed by samples pre-treatment. Samples were mixed with NaOH (1%, 2% and 3%) at 80 °C and agitated at approximately 700 rpm. The process was continued with neutralization process, filtration process and ended with analysis using UV-Vis Spectroscopy at 265 nm wavelengths. The results showed that the highest glucose concentration extracted was from mango peel waste (4.752 mg/mL) with 2% of NaOH in 3 grams mass loading, followed by banana peel waste (3.824 mg/mL) and pineapple peel waste (2.179 mg/mL) with 3% of NaOH in 5 grams and 6 grams mass loading respectively. Meanwhile, 4 grams of pineapple peel waste in 1% of NaOH resulted to the lowest glucose concentration (0.992 mg/mL). It is shown that the concentration of glucose extracted from the fruit peel increases with increasing mass loading and alkaline concentration increase

Extraction of Glucose by Supported Liquid Membrane using Polyethersulfone Flat Sheet Membrane Support

Sugar is released during biomass hydrolysis together with unwanted inhibitory compounds such as acetic acid, furfural and hydromethylfurfural. The extraction of particular sugar prior to fermentation process is needed in order to increase the yield of biofuel generated from biomass resources. In the current study, glucose was extracted from the aqueous solution using supported liquid membrane (SLM) system. Polyethersulfone (PES) flat sheet membrane support was fabricated using 15 wt.% PES, 42.5 wt.% DMAc and 42.5 wt.% PEG 200. Liquid membrane was formulated using 2-ethyl hexanol and methyl cholate as solvent and carrier, respectively. The effect of several parameters involved in SLM system such as type of diluent and flowrate of feed and stripping phase on the extraction performance of glucose were studied. About 52.77% of glucose was successfully extracted from aqueous solution using SLM system with 0.01M of methyl cholate in 2-ethyl hexanol. Using simulated biomass hydrolysate solution, almost 54.55% of glucose and 51.08% of xylose were successfully extracted using the SLM system.

Fast probing of glucose and fructose in plant tissues via plasmonic affinity sandwich assay with molecularly-imprinted extraction microprobes

Determination of specific target compounds in agriculture food and natural plant products is essential for many purposes; however, it is often challenging due to the complexity of the sample matrices. Herein we present a new approach called plasmonic affinity sandwich assay for the facile and rapid probing of glucose and fructose in plant tissues. The approach mainly relies on molecularly imprinted plasmonic extraction microprobes, which were prepared on gold-coated acupuncture needles via boronate affinity controllable oriented surface imprinting with the target monosaccharide as the template molecules. An extraction microprobe was inserted into plant tissues under investigation, which allowed for the specific extraction of glucose or fructose from the tissues. The glucose or fructose molecules extracted on the microprobe were labeled with boronic acid-functionalized Raman-active silver nanoparticles, and thus affinity sandwich complexes were formed on the microprobes. After excess Raman nanotags were washed away, the microprobe was subjected to Raman detection. Upon being irradiated with a laser beam, surface plasmon on the gold-coated microprobes was generated, which further produced plasmon-enhanced Raman scattering of the silver-based nanotags and thereby provided sensitive detection. Apple fruits, which contain abundant glucose and fructose, were used as a model of plant tissues. The approach exhibited high specificity, good sensitivity (limit of detection, 1 μg mL−1), and fast speed (the whole procedure required only 20 min). The spatial distribution profiles of glucose and fructose within an apple were investigated by the developed approach.

Towards Non-Invasive Extraction and Determination of Blood Glucose Levels.

Diabetes is a condition where the body is incapable of proper utilization of glucose and one that, if not properly managed, can lead to critical illness. Glucose monitoring and decision support is vital in avoiding potential adverse health effects. Current methods mainly involve invasive blood extraction for the purposes of blood glucose level notification, yet such methods rely on active user participation and subjective interpretation of the result. This paper reviews existing research in methods of extraction and monitoring of glucose levels. The purpose of this paper is to examine blood glucose extraction methods in addition to indicators of blood glucose level, toward development of an innovative, non-invasive extraction technology. Decision support methods are also analyzed toward customized, automated, and intelligent diabetic management.

The role of glucose, insulin and NEFA in regulating tissue triglyceride accumulation: Substrate cooperation in adipose tissue versus substrate competition in skeletal muscle

Background and aimsMetabolic factors initiating adipose tissue expansion and ectopic triglyceride accumulation are not completely understood. We aimed to investigate the independent role of circulating glucose, NEFA and insulin on glucose and NEFA uptake, and lipogenesis in skeletal muscle and subcutaneous adipose tissue (SCAT). Methods and resultsTwenty-two pigs were stratified according to four protocols: 1) and 2) low NEFA + high insulin ± high glucose (hyperinsulinaemia–hyperglycaemia or hyperinsulinaemia–euglycaemia), 3) high NEFA + low insulin (fasting), 4) low NEFA + low insulin (nicotinic acid). Positron emission tomography with [18F]fluoro-2-deoxyglucose and [11C]acetate, was combined with [14C]acetate and [U-13C]palmitate enrichment techniques to assess glucose and lipid metabolism.Hyperinsulinaemia increased glucose extraction, whilst hyperglycaemia enhanced glucose uptake in skeletal muscle and SCAT. In SCAT, during hyperglycaemia, elevated glucose uptake was accompanied by greater [U-13C]palmitate-TG enrichment compared to the other groups, and by a 39% increase in de novo lipogenesis (DNL) compared to baseline, consistent with a 70% increment in plasma lipogenic index. Conversely, in skeletal muscle, [U-13C]palmitate-TG enrichment was higher after prolonged fasting. ConclusionsOur data show the necessary role of hyperglycaemia–hyperinsulinaemia vs euglycaemia–hyperinsulinaemia in promoting expansion of TG stores in SCAT, by the consensual elevation in plasma NEFA and glucose uptake and DNL. In contrast, skeletal muscle NEFA uptake for TG synthesis is primarily driven by circulating NEFA levels. These results suggest that a) prolonged fasting or dietary regimens enhancing lipolysis might promote muscle steatosis, and b) the control of glucose levels, in association with adequate energy balance, might contribute to weight loss.

A selective glucose sensor based on direct oxidation on a bimetal catalyst with a molecular imprinted polymer

A selective nonenzymatic glucose sensor was developed based on the direct oxidation of glucose on hierarchical CuCo bimetal-coated with a glucose-imprinted polymer (GIP). Glucose was introduced into the GIP composed of Nafion and polyurethane along with aminophenyl boronic acid (APBA), which was formed on the bimetal electrode formed on a screen-printed electrode. The extraction of glucose from the GIP allowed for the selective permeation of glucose into the bimetal electrode surface for oxidation. The GIP-coated bimetal sensor probe was characterized using electrochemical and surface analytical methods. The GIP layer coated on the NaOH pre-treated bimetal electrode exhibited a dynamic range between 1.0µM and 25.0mM with a detection limit of 0.65±0.10µM in phosphate buffer solution (pH 7.4). The anodic responses of uric acid, acetaminophen, dopamine, ascorbic acid, L-cysteine, and other saccharides (monosaccharides: galactose, mannose, fructose, and xylose; disaccharides: sucrose, lactose, and maltose) were not detected using the GIP-coated bimetal sensor. The reliability of the sensor was evaluated by the determination of glucose in artificial and whole blood samples.

Preparation and adsorption properties of glucose molecularly imprinted polymers in hydrous solution for effective determination of glucose in fruits by MISPE–HPLC

In this paper, a kind of surface molecular imprinting polymers in hydrous solution, with glucose selectively recognition, was successfully synthesized by surface molecular imprinting method, using glucose (Glu) as template molecule, acrylamide as functional monomers, N,N′-methylenebisacrylamide as the cross-linking agent, ammonium peroxydisulfate as the initiator, activated silica gel (SiO2@NH2) as support particles. The influences of cross-linker, initiator as well as support particles amount on the adsorption capacity of Glu-MIPs were performed by single-factor experiments. The optimum conditions were 100 mg of cross-linker, 25 mg of initiator and 1 g of SiO2@NH2. The adsorption and thermodynamics research revealed that the adsorption of MIPs was fitted to Langmuir, maximum imprinting factor of 2.49 and maximum absorption capacity of 50.06 mg/g. Furthermore, a procedure of extraction of glucose from real fruits samples using the Glu-MIPs as solid-phase extraction adsorbent was developed to apply in analytical techniques.

Does cord glucose extraction ratio discriminate the risk of early neonatal hypoglycaemia?

Does cord glucose extraction ratio discriminate the risk of early neonatal hypoglycaemia?

Relationship between masticatory performance using a gummy jelly and masticatory movement

PurposeThe purpose of this study was to clarify the relationship between masticatory performance using a gummy jelly and masticatory movement. MethodsThirty healthy males were asked to chew a gummy jelly on their habitual chewing side for 20s, and the parameters of masticatory performance and masticatory movement were calculated as follows. For evaluating the masticatory performance, the amount of glucose extraction during chewing of a gummy jelly was measured. For evaluating the masticatory movement, the movement of the mandibular incisal point was recorded using the MKG K6-I, and ten parameters of the movement path (opening distance and masticatory width), movement rhythm (opening time, closing time, occluding time, and cycle time), stability of movement (stability of path and stability of rhythm), and movement velocity (opening maximum velocity and closing maximum velocity) were calculated from 10 cycles of chewing beginning with the fifth cycle. The relationship between the amount of glucose extraction and parameters representing masticatory movement was investigated and then stepwise multiple linear regression analysis was performed. ResultsThe amount of glucose extraction was associated with 7 parameters representing the masticatory movement. Stepwise multiple linear regression analysis showed that the opening distance, closing time, stability of rhythm, and closing maximum velocity were the most important factors affecting the glucose extraction. ConclusionFrom these results it was suggested that there was a close relation between masticatory performance and masticatory movement, and that the masticatory performance could be increased by rhythmic, rapid and stable mastication with a large opening distance.

A potentiometric non-enzymatic glucose sensor using a molecularly imprinted layer bonded on a conducting polymer

A non-enzymatic potentiometric glucose sensor for the determination of glucose in the micomolar level in saliva was developed based on a molecularly imprinted polymer (MIP) binding on a conducting polymer layer. A MIP containing acrylamide, and aminophenyl boronic acid, as a host molecule to glucose, was immobilized on benzoic acid-functionalized poly(terthiophene) (pTBA) by the amide bond formation onto a gold nanoparticles deposited-screen printed carbon electrode (pTBA/AuNPs/SPCE). Aromatic boronic acid was incorporated into the MIP layer to stably capture glucose and create a potentiometric signal through the changed pKa value of polymer film by the formation of boronate anion-glucose complex with generation of H+ ions by the cis-diol reaction. Reversible binding and extraction of glucose on the sensor surface was observed using a quartz crystal microbalance. Each layer of the sensor probe was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, X-ray photoelectron spectroscopy, and atomic force microscopy. The potentiometric response at the optimized conditions exhibited a wide linear dynamic range of 3.2×10−7 to 1.0×10−3M, with a detection limit of 1.9 (±0.15)×10−7M. The sensor probe revealed an excellent selectivity and sensitivity for glucose compared to other saccharides. In addition, the reliability of the proposed glucose sensor was evaluated in physiological fluid samples of saliva and finger prick blood.

Performance analysis of bioethanol (Water Hyacinth) on diesel engine

ABSTRACTThe increasing consumption and excessive extraction of conventional fuels is the matter of serious concern. Nowadays, world is looking for alternative sources of fuel which can partially replace conventional fuel dependence. The current investigation intends to provide evaluation of bio-ethanol preparation from Water Hyacinth (WH) and its influence on diesel engine performance under various operating conditions. This study explores the extraction of glucose from WH (Eichhornia crassipes) pretreated with sulfuric acid (H2SO4) for production of bio-ethanol. For the production of bio-ethanol different concentrations of H2SO4 acid hydrolysate (1%, 2%, 4%, 6%, 8%, and 10%) were prepared which was then followed by fermentation with cellulose fermenting yeasts. From results, it was observed that 4% H2SO4 acid hydrolysis produces higher concentrations of ethanol than other concentrations. Bio-ethanol extracted from WH was blended with diesel in different proportions (5%, 10%, 15%, 20%, and 25%) v/v and performance and emissions were experimentally investigated on single cylinder diesel engine under various load conditions. Experimental results show that 5 BED [5% bio-ethanol (WH + 95%diesel v/v) and 10BED (10% bio-ethanol (WH + 90%diesel v/v)] produces higher brake power, brake thermal efficiency and brake mean effective pressure with improved exhaust emission profiles than any other blend.

The Effects of Capillary Transit Time Heterogeneity (CTH) on the Cerebral Uptake of Glucose and Glucose Analogs: Application to FDG and Comparison to Oxygen Uptake.

Glucose is the brain's principal source of ATP, but the extent to which cerebral glucose consumption (CMRglc) is coupled with its oxygen consumption (CMRO2) remains unclear. Measurements of the brain's oxygen-glucose index OGI = CMRO2/CMRglc suggest that its oxygen uptake largely suffices for oxidative phosphorylation. Nevertheless, during functional activation and in some disease states, brain tissue seemingly produces lactate although cerebral blood flow (CBF) delivers sufficient oxygen, so-called aerobic glycolysis. OGI measurements, in turn, are method-dependent in that estimates based on glucose analog uptake depend on the so-called lumped constant (LC) to arrive at CMRglc. Capillary transit time heterogeneity (CTH), which is believed to change during functional activation and in some disease states, affects the extraction efficacy of oxygen from blood. We developed a three-compartment model of glucose extraction to examine whether CTH also affects glucose extraction into brain tissue. We then combined this model with our previous model of oxygen extraction to examine whether differential glucose and oxygen extraction might favor non-oxidative glucose metabolism under certain conditions. Our model predicts that glucose uptake is largely unaffected by changes in its plasma concentration, while changes in CBF and CTH affect glucose and oxygen uptake to different extents. Accordingly, functional hyperemia facilitates glucose uptake more than oxygen uptake, favoring aerobic glycolysis during enhanced energy demands. Applying our model to glucose analogs, we observe that LC depends on physiological state, with a risk of overestimating relative increases in CMRglc during functional activation by as much as 50%.

Comparison of techniques for culture of dialysis water and fluid.

Microbiological culture of dialysis water and fluid is a routine safety measure. In the United States (U.S.), laboratories perform these cultures on trypticase soy agar at 35-37°C for 48 h (TSA-48h), not on the tryptone glucose extract agar or Reasoner's 2A agar at 17-23°C for 7 days (TGEA-7d and R2A-7d, respectively) recommended by international standards. We compared culture methods to identify samples exceeding the accepted action level of 50 CFU/mL. Dialysis water and fluid samples collected from 41 U.S. dialysis programs between 2011 and 2014 were cultured at two U.S. laboratories. Each sample was cultured using (1) either TGEA-7d or R2A-7d and (2) TSA-48h. We compared proportions exceeding the action level by different methods and test characteristics of TSA-48h to those of TGEA-7d and R2A-7d. The proportion of water samples yielding colony counts ≥50 CFU/mL by TGEA-7d was significantly different from the proportion by TSA-48h (P = 0.001; difference in proportion 4.3% [95%CI 1.3-7.3%]). The proportions of dialysis fluid samples ≥50 CFU/mL by TGEA-7d and TSA-48h were not significantly different; there were no significant differences for comparisons of R2A-7d to TSA-48h. In dialysis fluid, TSA-48h was comparable to TGEA-7d and R2A-7d in identifying samples as having bacterial counts ≥50 CFU/mL. In dialysis water, TSA-48h was comparable to R2A-7d in identifying samples ≥50 CFU/mL, but TGEA-7d did yield significantly more results above 50 CFU/mL. Nonetheless, the negative predictive value of a TSA-48h result of <50 CFU/mL in dialysis water exceeded 95%.

Low serum miR-320b expression as a novel indicator of carotid atherosclerosis

We aimed to investigate correlation of carotid plaque with serum microRNA level and provide a potential mechanism for ischemic stroke prevention. Fasting serum was obtained from 177 patients with carotid plaques diagnosed using B ultrasound and 155 healthy subjects for RNA extraction and measurement of glucose, lipid and related biochemical indices. microRNAs level was assayed using microarray, validated using real-time PCR and statistically analyzed for their correlation with relevant clinical information. Statistical analyses showed significant differences between control and plaque groups in age, smoking history, and histories of cerebrovascular diseases, blood pressure, diabetes and coronary diseases. Patients in plaque group had significantly higher LDL-C level but significantly lower miR-320b level than healthy subjects. The P50 (P25–P75) of miR-320b was 2.38 (1.46–3.80) in control group and 1.20 (0.80–2.01) in plaque group (P=0.000), as well as 1.39 (0.83–2.08) in stable plaque group and 0.77 (0.590.99) in vulnerable plaque group (P=0.000). The area under the ROC curve of traditional factors, miR320b with traditional factors, traditional factors with plaque diameter, and traditional factors with IMT for patients with carotid plaques was 0.710, 0.834, 0.826 and 0.808, respectively. This study suggests that miR-320b is a specific serum marker of carotid atherosclerosis and vulnerable plaque, and its combination with traditional factors is the best means for diagnosis of cerebrovascular diseases. The carotid atherosclerosis complicated with vulnerable plaque is a high risk factor for cerebral infarction. B-ultrasound examination combined with serum miR-320b expression and traditional indices could be used to predict subjects with high-risk for ischemic stroke.

Exobiopolymer production of Phytocordyceps sp. BCC 2744 and Akanthomyces pistillariiformis BCC 2694; optimization and scale-up

Production of exobiopolymers by Phytocordyceps sp. BCC 2744 and Akanthomyces pistillariiformis BCC 2694, interleukin-8 (IL-8) inducers, were optimized using experimental design. Biological and physiological properties of these exobiopolymers are attractive to use as wound-dressing material, so that it is very interesting to increase its production. Phytocordyceps sp. BCC 2744 and Akanthomyces pistillariiformis BCC 2694 were cultivated on different carbon and nitrogen sources and the best carbon and nitrogen sources for exobiopolymer production of Phytocordyceps sp. BCC 2744 were glucose and peptone, respectively, and 0.88 g/L exobiopolymer was obtained. Higher exobiopolymer (1.82 g/L) was obtained on glucose and meat extract by Akanthomyces pistillariiformis BCC 2694. After the effects of 4 variables were studied using a two-level fractional design, glucose and peptone concentration were the most influential parameters on exobiopolymer production of Phytocordyceps sp. BCC 2744. Lower exobiopolymer production was obtained in the medium supplemented with 5-Fluorouracil and vitamin solution at high level (10 mM and 3mL/L, respectively). The highest exobiopolymer production was obtained on 60 g/L glucose and 20 g/L peptone and 2.32 g/L exobiopolymer was produced. About 4.0 g/L exobiopolymer production in a 20 L bioreactor was obtained. At least 4 variables; medium type, nitrogen sources, glucose, and nitrogen concentration were applied on a two-level factorial design of biomass and exobiopolymer production of Akanthomyces pistillariiformis BCC 2694. Models obtained from the multiple regression are significant. At high level of glucose (60 g/L), phosphate medium, and 20 g/L meat extract, higher exobiopolymer production and about 2.54 g/L was obtained. About 4.5 g/L exobiopolymer production in 20 L bioreactor was obtained.

Selection and development of efficient consortia for decolorization of metal complex dyes

ABSTRACTDecolorization of 18 different metal complex acid dyes was studied with 11 bacterial consortia. Five consortia and five dyes were selected on the basis of maximum decolorization. Among the parameters for optimization, nutrient broth was preferred by all bacterial consortia but one of them showed significant decolorization with Bushnell and Haas medium with or without glucose and yeast extract; hence, this consortium was selected for further studies. Decolorization efficiency was adversely affected beyond 200 mg/L dye and 2% NaCl concentration. Treatment efficiency was increased 2-fold using a developed consortium as compared to original consortium. Changes in the UV-Vis spectra and considerable reduction in chemical oxygen demand of selected metal complex dyes after decolorization prove the efficiency of the consortium for its use. Decolorization of a broad range of metal complex dyes at pH 7.5 ± 0.5, 35 ± 2 °C, and static conditions promises the use of the developed consortium for industrial wastewater treatment.

Data Collection Network --Defining a Pattern of Acute Biomarkers in Human Cord Blood for HIE Risk at Birth

We have developed a program seeking an expanded human cord blood data set by employing online registration through a website with funding via Kickstarter and seeking Ad Hoc grants for clinical centers registering cases. At PAS 2013 Abstract# (755783) we reported the initial use of biomarkers in a fully Translational two species analysis which superimposes values from human cord blood onto the precisely defined pattern of changes in Fetal Glucose Extraction and Arterial pH generated in fetal sheep with known durations of umbilical occlusion and subsequent absolute risk of neuronal loss with Hypoxic Ischemic Encephalopathy (HIE). Through personal contacts recruitment will occur for self-selected clinical centers; …

Detection and Molecular Identification of Persistent Water Vessel Colonizing Bacteria in a Table Water Factory in Nigeria

Aims: To establish organisms persistent in a table water production facility and to determine the points they entered in order to eliminate any risk of having pathogens in a water production system. Study Design: Analytic observational studies Place and Duration of Study: Bottling facility in Nigeria and University of Nottingham, United Kingdom. Study was between August 2011 and Sept 2013. Methodology: Sample control points were subjected to counts of bacteria, yeasts, and mold using membrane filtration to trace the source of increased bacteria counts in a table water production factory. Organisms were identified by 16S rRNA sequencing and biofilm formation was assessed Original Research Article Nwaiwu and Nwachukwu; BMRJ, 13(5): 1-12, 2016; Article no.BMRJ.24378 2 with micro titer dish biofilm formation analysis. Results: Total bacteria and Pseudomonas Spp. count were highest in the carbon filter and 5% (v/v) chlorine used for disinfection was found to be effective against planktonic cells of 18 hour cultures of Pseudomonas Spp. isolated from the carbon filter tank. No yeasts and mold were detected and after a sand blasting exercise to clean affected tanks, total bacteria counts on tryptone glucose extract medium decreased to less than factory allowable limits of 25 cfu per 100 ml of water with no further growth of Pseudomonas Spp. on centrimide medium. Other bacteria that emerged after the sand blasting exercise were regarded as persistent but had less biofilm forming ability (p=.02) when compared with Pseudomonas aeruginosa PAO1. The persistent bacteria identified after sequencing were Aeromonas hydrophila and Serratia proteamaculans. Conclusion: Apart from Pseudomonas Spp., other bacteria can persist in the water tanks of a water bottling facility and routine checks may fail to detect an underlying problem until it becomes obvious. Prompt corrective action ensures that public safety is not compromised. This was the first time Aeromonas hydrophila and Serratia proteamaculans were identified from the water tanks in the bottling facility.

Microbial Degradation of Reactive Orange M2R Dye by Bacterial Consortium ETL-A

Textile and Textile dyestuff industries discharge effluent having high Chemical Oxygen Demand (COD) and colour, making it difficult to treat the effluent completely. Several Physical, Chemical and Biological treatment methods are available to treat such an effluent. However, in recent year’s biological treatment with focus on bacteria have been drawing a tremendous attention due to their ability to degrade complex structured dyes and hence treat waste water. In the present study, Soil and Water samples collected from textile dye effluent contaminated sites of Ankleshwar, Gujarat was studied for screening and isolation of bacteria capable of decolorizing and degrading textile dyes. A bacterial consortium ETL-A was selected on the basis of rapid dye decolorization. Bacterial consortium exhibited 93% decolorization ability within 30 h under static conditions at 35oC in presence of Glucose and Yeast Extract as cosubstrates. 16S rRNA gene amplification was carried out for the sequencing and identification of these strains. The degradation of dye was confirmed by HPTLC and FTIR analysis. Considerable decrease in COD of the dye (above 85%) was indicative of conversion of complex dye into simple oxidizable products.

Microbiological Analysis of Hemodialysis Water at the University Teaching Hospital of Yaounde, Cameroon

Rigorous control of the microbiological quality of water in hemodialysis services is important because the immune system of patients with chronic renal failure is weakened. The objective of this study was to determine the microbiological quality of water for hemodialysis in the hemodialysis department of the University Teaching Hospital of Yaounde in order to improve the disinfection strategy. Twelve water samples were collected each month at different sites of the hemodialysis circuits A (inlet of filters), B (Outlet of filters / inlet of Reverse Osmosis (RO) device) and C (outlet of the RO device / close to the generator) between July and October 2015 to be analyzed. The bacteria were isolated after filtration of 100 ml of water at each site through nitrocellulose membrane with 0.45 µm microporosity deposited on the surface of the Tryptone Glucose Extract Agar (TGEA) and then incubated at room temperature (20 to 22°C) for 7 days. After transplanting to different environments, pure bacterial isolates were identified by their cultural characters and marketed biochemical galleries. The colony count was well above the required international standards (>100 CFU / ml), for the hemodialysis water with a percentage of 83.3% (10/12) of non-compliance. Among the bacteria identified, nine (09) were Gram-negative bacilli including Pasteurella haemolytica, Pseudomonas fluorescens, Pseudomonas paucimobilis, Aeromonas salmonicida and Klebsiella pneumoniae subsp ozaenae, three (03) Gram-positive bacilli all Bacillus sp and six (06) Gram-positive cocci all of coagulase-negative staphylococci. The most frequently isolated bacterial genera were Pseudomonas (30.4%), Staphylococcus (26.1%), Aeromonas (13%), Bacillus (13%), Klebsiella (13%) and Pasteurella (4.3%). In this study, the high bacteriological contamination of the hemodialysis water with the detection of a variety of bacteria shows that the disinfection procedure of the distribution loop is not efficient and cannot prevent the development of a biofilm. A higher frequency of disinfection (almost every week), an increase of the concentration and time of contact of the chlorine disinfection product or the use of peracetic acid and a regular monitoring can contribute to improve the quality of the hemodialysis water at the CHUY to ensure a better quality of life for patients undergoing this treatment.