Gloma Research Articles

Expression and clinical significance of MGMT, Ki-67 and P53 in human gliomas

Objective To detect the expression of DNA repair enzyme O6-methylguanine DNA methyltransferase (MGMT), cell proliferation-related nuclear protein (Ki-67) and P53 protein in gliomas, and investigate the relationship and clinical significance among them and gloma grade. Methods 61 cases of brain glioma specimes and 16 cases of internal decompression of brain trauma were used to detect the expression of MGMT, Ki-67 and P53 by using immunohistochemical SP method. Results MGMT protein expression (19.67%∶0, χ2=3.729, P=0.062), Ki-67 protein expression (39.34%∶0, χ2=5.722, P=0.016) and P53 protein expression (27.87%∶0, χ2=9.146, P=0.002) showed significant differences in gliomas compared to normal brain tissues, the expression of Ki-67 was significantly higher in high-grade gliomas(Ⅲ-Ⅳ) than that in low-grade gliomas (Ⅰ-Ⅱ) (14∶10, χ2=11.718, P=0.001). No significant difference was found between the MGMT and P53. Conclusion MGMT protein can be used as a biomarker in gliomas detection; Ki-67 has a positive correlation with tumor grade, and can be used as a reference indicator of pathological grade; P53 protein expression may be used as a potential target for the treatment of gliomas. Key words: Glioma; O6-methylguanine DNA methyltransferase; Cell proliferation-related nuclear protein Ki-67; Tumor suppressor protein p53

Redioresistence of glioma stem cell in U251 cell line

Objective To explore the radioresistence of glioma stem cell iso-lated from U251 cell line.Methods U251 cells were cultured in serum-free DMEM/F12 medium,There were forming U251 brain tumor spheres,In order to observe the characteristic of the cell of tumor spheres,the cell of tumor spheres were identifyed by immunohistochemistry and were irradiated by X-rays with different doses,The activ-ity of cell were measured by methyl thiazol tetrazolium (MTT),the marker CD133 and ABCG2 were measured by Flow cytometer.Results Some U251 glioma cells have the capacity to self-renew and generate free-floating brain tumor spheres; CD133 + cells were 3.17％ and ABCG2 ± cells were 1.17％ in tumur sphere,After 9Gy irradiated,CD133 + cells increased to13.24％ and ABC-G2 + cells were 8.76％ ; After ir-radiated,the activity of cells decreased obviously.Conclusion Glioma stem cell can achieve bv cultured in serum-free medium and Glioma stem cell show more radioresistance than glioma cell. Key words: U251 glima cell line; Gloma stem cell; Irradiation

Radiologic features of the chordoid glioma: a report of three cases

Objective To study the radiographic features of the chordoid glioma and its differential diagnosis,and increase neuroradiologists'awareness of this newly described tumor,facilitating prospective diagnosis.Methods Three patients with chordoid glioma were reviewed retrospectively to determine whether any characteristic radiographic findings and clinical-pathologic findings would emerge,and the relevant literatures were reviewed.Routine CT(2 cases)and MR(3 cases),contrast-enhanced MR(3 cases)were performed.Results The masses were well circumscribed and located in the region of the hypothalamus/third ventricle-suprasellar region(2 cases)and intrasuprasellar region(1 case).Tumors were isointense to gray matter on CT scans and were isointense or hypointense on T1WI and hyperintense on T2WI.In two patients,vasogenic edema extended into the optic tracts and cystic or necrotic area was seen.All cases were remarkably enhanced following contrast administration.Conclusions Radiologic findings of the chordoid glioma has some unique features.Tumor,in the region of the hypothalamus-third ventricle-suprasellar region,if it is well circumscribed and remarkably enhancing following contrast administration,should be included in the differential diagnosis. Key words: Gloma; Magnetic resonance imaging; Tomography,X-ray computed