Globule Diameter Research Articles

Embryonic and larval development of black jewfish, Protonibea diacanthus: A new candidate for aquaculture in northern Australia

Black jewfish Protonibea diacanthus is a large, commercially important fish distributed across the tropical Indo-Pacific. In Australia, there is an established commercial wild fishery for the species but it’s aquaculture potential has not been investigated. This research presents hatchery production of native P. diacanthus in Darwin, Australia and documents ontogeny of early life stages of this species over three trial runs. Wild P. diacanthus were induced to spawn with luteinizing hormone-releasing hormone analog (LHRHa) and eggs were released approximately 25–28 h after injection (0.88 ± 0.01 mm in diameter with oil globule 0.24 ± 0.01 mm). Larvae hatched 12 h after spawning. At one day post hatch (dph) larvae were 2.68 ± 0.01 mm standard length with 0.15 ± 0.01 mm oil globule diameter. Larvae mouths opened at 31 h post hatch (hph) and first feeding was seen at 57 hph. Swim bladder inflation (92 %) occurred between 40 and 62 hph. Larvae were progressively fed rotifers, Artemia and Otohime microdiet, and metamorphosis occurred between 20 and 25 dph, completing as they reached over 13 mm standard length. Tail biting cannibalism began with the onset of metamorphosis and caused significant mortality until approximately 24 dph when metamorphosed juveniles could be handled and moved to low stocking density systems. This study demonstrates that northern Australian P. diacanthus is amenable to hatchery production and provides important foundational knowledge for this emerging aquaculture species.

Study on Single Nucleotide Polymorphism of LAP3 Gene and Its Correlation with Dairy Quality Traits of Gannan Yak.

This study explored the polymorphism of the leucine aminopeptidase (LAP3) gene and its relationship with milk quality characteristics in Gannan yak. A cohort of 162 Gannan yak was genotyped utilizing the Illumina Yak cGPS 7K BeadChip, and the identified single nucleotide polymorphisms (SNPs) were evaluated for their association with milk protein, casein, lactose, and fat concentrations. The results showed that four SNPs (g.4494G > A, g.5919A > G, g.8033G > C, and g.15,615A > G) in the LAP3 gene exhibited polymorphism with information content values of 0.267, 0.267, 0.293, and 0.114, respectively. All four SNPs were in Hardy-Weinberg equilibrium (p > 0.05). The g.4494G > A and g.5919A > G SNPs were significantly associated with protein content (p < 0.05), with homozygous genotypes showing significantly higher protein content than heterozygous genotypes (p < 0.05). The g.8033G > C SNP was significantly associated with casein content, protein content, non-fat solids, and acidity (p < 0.05), with the CC genotype having significantly higher casein, protein, and non-fat solids content than the GG and GC genotypes (p < 0.05). The g.15,615A > G SNP was significantly associated with average fat globule diameter (p < 0.05). In general, the mutations within the LAP3 gene demonstrated a positive impact on milk quality traits in Gannan yak, with mutated genotypes correlating with enhanced milk quality. These results indicate that the LAP3 gene could be a significant or candidate gene affecting milk quality traits in Gannan yak and offer potential genetic markers for molecular breeding programs in this species.

Impact of milking interval and time on milk spontaneous lipolysis and composition in dairy cows

Milk lipolysis is defined as the hydrolysis of triglycerides, the major component of milk fat, resulting in the release of short-chain fatty acids (FA) responsible for rancid flavor and partial glycerides that impair functional properties such as foaming and creaming abilities. Milk lipolysis is a complex phenomenon that depends on both animal parameters and breeding factors. Milk spontaneous lipolysis is known to be higher in milk from evening milkings than from morning milkings. This may be related to the longer length of overnight milking intervals or to the nycthemeral cycle. In this experiment, our objective was thus to study the impact of both milking intervals and time of day on milk spontaneous lipolysis in twice-daily-milking systems with one of 3 milking intervals: Short Day - Long Night (SD-LN, 6.30 a.m. and 4.30 p.m.,); Long Day - Short Night (LD-SN, 6:30 a.m. and 8:30 p.m.,); and Balanced Day and Night (BDN, 6:30 a.m. and 6:30 p.m.,). To achieve this goal, 21 multiparous dairy cows in mid-lactation were used in a 3 × 3 Latin square design over 3 periods. The experiment lasted 5 weeks, corresponding to 3 experimental periods of 6 d alternating with 8 d of milking with conventional hours (morning-evening gap of 10 h). We confirmed that milk spontaneous lipolysis was influenced by milking interval, but not the milking time. Indeed, we observed more lipolysis in SD-LN evening milk (+0.20 mEq/100 g fat) and LD-SN morning milk (+0.22 mEq/100 g fat), both of which corresponded to a 10 h interval between successive milkings. High lipolysis milk came from cows that produced less milk with a higher milk fat content. No significant difference between milkings was observed for BDN. Milk protein, total P and citrate contents increased according to the duration of mammary gland storage of milk (from 10 to 14 h). There was no effect of milking intervals on milk fat globule diameter. The milk Na+/K+ ratio, indicating an opening of tight junctions in the mammary gland, increased only in evening milkings with BDN and LD-SN. In conclusion, we found that the effect of milking intervals on lipolysis is stronger than that of the nycthemeral cycle.

Study on storage technology of corn wax based ice cream

Abstract Ice cream is a complex food colloidal system; saturated fat is an essential element given the taste and texture of ice cream (viscosity, hardness). In recent years, research on fat ice cream has focused on adding fat substitutes. Looking for a suitable source of lipid-based fat substitute instead of fat ice cream, developed more conducive to human health, low in saturated / zero trans fatty ice cream products. The study will use linoleic acid-rich soybean and corn oil gel waxes as a fat source for ice cream, make gel-oil-based ice cream, and study changes in its quality during storage. With an incremental storage time index, we can inspect the effects of different storage times on melting rate, hardness, and fat globule diameter changes. The hardness and melt rates show an increasing trend while the fat globule diameter decreases. We found that the quality of ice cream has reduced over time, but it does not affect the sensory evaluation. By analyzing the acidity, peroxidation value, and F.A. composition changes, we found that the acidity and peroxidation values are low, indicating that the shelf-life of ice cream gel is higher due to using better quality fresh oil. The fatty acid composition did not change during storage, and no trans fatty acids were produced.

Fat globule diameter in infant formulas

ObjectivesThe lipid fraction, fatty acid profile, and diameter of fat globules of infant formulas show great differences from human milk. These characteristics influence fat digestion and, consequently, the development and health of infants. The aim of this study was to evaluate the diameter of fat globules in infant formulas and compare them with those in human milk. MethodsThe diameter of fat globules of 10 infant formulas and human milk samples was determined using scanning electron microscopy. ResultsThe starter infant formula was the only one that showed a mean diameter value (3.52 ± 2.17 µm) similar to that obtained for human milk (3.44 ± 1.68 µm). The starter infant formula showed the highest values of volume-surface D3,2 (6.13 µm) and volume-weighted D4,3, (7.05 µm) mean diameters among the infant formulas analyzed, and close to those obtained for the human milk sample (5.16 and 5.98 µm, respectively). The infant formulas whey protein partially hydrolyzed, soy protein isolate-based, whey protein extensively hydrolyzed, and thickened with pregelatinized starch had the lowest mean diameters of fat globules 0.64 ± 0.22, 0.70 ± 0.19, 1.06 ± 0.34, and 1.22 ± 0.48 µm, respectively. ConclusionThe analysis of principal components showed that none of the analyzed infant formulas had similarity with the diameter of fat globules and the fatty acid profile of human milk.

Benchmarking of early ontogeny parameters as predictors of the first-feeding larvae vitality in spotted rose snapper Lutjanus guttatus

A common problem in marine fish hatcheries is the lack of functional embryonic parameters reliably predicting favorable larval outcomes. Embryos characterized as high-quality by typical assessment methods frequently result in weak larvae with high mortality during the endo-exotrophic transition. Thus, because there is no universal consensus on what parameters are most associated with successful larval survivability, this study aims to determine the most reliable, practical, and real-time predictor of vitality at the earliest developmental stage for first-feeding larvae of L. guttatus. From 18 mass volitional spawnings, the reproductive performance and quality of embryos and larvae were assessed by following quality parameters: total fecundity per spawning, percentage of floating eggs, fertilization rate, blastomere morphology score (at 8–32 cell stages), egg and oil globule volumes, coefficient of variation of eggs and oil globule diameters, percentage of poly-globular eggs, oil globule-to-egg volume percentage, morphological appearance and survival rates at 24 (hatching), 48 and 72 h post-spawning, larvae transition rate, and survival activity index (SAI). SAI was used as a larval vitality benchmark to determine the predictive capacity of the abovementioned parameters. Our results show that blastomere morphology score was the only parameter positively correlated with SAI. However, when examining each component of blastomere morphology to SAI prediction, only the presence of cell inclusions, but not size, symmetry, margins, or adhesions of blastomeres, predict the SAI. This reveals that embryos with defective blastomeres can develop into normal-appearing larvae but are weak to starvation. Therefore, cell inclusions are the best real-time, early, reliable, and functional predictor of first-feeding larvae vitality in L. guttatus.

Corticosteroid plasma kinetics and gonadal receptor gene expression during the reproductive cycle in female Eurasian Perch: Investigation of the roles of corticosteroids in vitellogenesis.

To improve the quality of reproduction in Eurasian perch, Perca fluviatilis L., which is a promising candidate for Eurasian freshwater aquaculture that is currently cultivated in recirculating aquaculture systems (RAS), investigating the hormones that mediate and affect reproduction in this species is indispensable. The literature defines a group of four major corticosteroids (11-deoxycorticosterone, 11-deoxycortisol, corticosterone and cortisol) that might mediate critical stages of reproduction in female perch. Unfortunately, neither the basic roles nor the kinetics of these four corticosteroids throughout the reproductive cycle of female perch have been well defined to date. In this study, we therefore elucidated the plasma kinetics of these four corticosteroids during the reproductive cycle of domesticated female perch while monitoring the expression of the different receptors and enzymes that mediate their production and possible functions. Additionally, we performed an invitro experiment during late vitellogenesis to investigate the possible direct roles of these steroids during that stage. Our results revealed that these four corticosteroids were detectable throughout the reproductive cycle, and the levels of most of them (11-deoxycorticosterone, 11-deoxycortisol, and cortisol) fluctuated significantly depending on the stage of reproduction. 11-Deoxycorticosterone and 11-deoxycortisol exhibited their highest levels, 1.8ng/ml and 58ng/ml, respectively, at the beginning of the reproductive cycle. By the end of the reproductive cycle, 11-deoxycortisol and cortisol plasma levels exhibited a surge, reaching 58ng/ml and 150ng/ml, respectively. During the perch reproductive cycle, the corticosteroid receptor complex is not regulated only at the hormone level, as the expression levels of all corticosteroid receptor genes showed a progressive and similar decline. In vitro exposure of vitellogenic oocytes to some of these corticosteroids (11-deoxycorticosterone and 11-deoxycortisol) induced an increase in yolk globule diameter and a decrease in the density of yolk globules, which indicates the involvement of both of these hormones in yolk globule coalescence. Taken together, these results implicate corticosteroids in the reproductive cycle, although the related cellular mechanisms remain to be investigated.

Feed restriction as a tool for further studies describing the mechanisms underlying lipolysis in milk in dairy cows

Milk lipolysis is defined as the hydrolysis of triglycerides, which are the main component of milk fat. Short-chain fatty acids (FAs) released in milk are responsible for rancid flavour. In addition, the presence of partial glycerides impairs the functional properties of milk, such as foaming and creaming abilities. Milk lipolysis, a key criterion used to assess milk quality, depends on animal parameters and breeding factors. Low-energy diets are associated with higher levels of spontaneous lipolysis, particularly in late lactation. In this study, dairy cows were fed a restricted diet (i.e. 65% of their ad libitum DM intake (DMI)) to induce spontaneous lipolysis in milk and to study milk composition associated with lipolysis. Two groups of 22 cows each received a control diet (100% of ad libitum DMI) or the restricted diet according to a 2 × 2 crossover design. The restricted diet was fed for five days. As expected, feed restriction increased milk spontaneous lipolysis which was associated with an increase in lipoprotein lipase activity. At the same time, milk yield and protein content decreased and no effect was observed on milk fat content. The increase in spontaneous lipolysis was associated with an increase in milk fat globules diameter, without influencing casein micelles diameter. Feed restriction altered the parameters of dairy cow metabolism, with increases in plasma non-esterified FAs, triglycerides and urea, indicating body fat mobilisation and protein catabolism associated with feed restriction. Feed restriction also altered hormonal parameters, with decreases in plasma insulin, insulin-like growth factor 1 and prolactin. As expected, lipolysis was higher in evening milk and was associated with a larger diameter of milk fat globules. This zootechnical approach will be completed with proteomic, lipidomic and transcriptomic studies of milk and/or mammary gland of animals selected for their extreme lipolysis.

Physicochemical and Rheological Properties of Stirred Yoghurt during Storage Induced from High-Intensity Thermosonicated Goat and Cow Milk

The effect of high-intensity thermosonication (HIT) pretreatment (20 kHz frequency, output power 4000 W and 25% amplitude for 5, 10 and 15 min) on the physicochemical and rheological properties of stirred yoghurt made from goat milk was studied. Various parameters of the milk were evaluated, such as the particle size, pH and soluble calcium and phosphorus, while other parameters of the stirred yoghurt were evaluated during storage (up to 18 days), such as the rheological measurements, syneresis, pH values, titratable acidity, color, and sensory properties. The microstructure had more interconnected chains than the stirred yoghurt made from homogenized milk on the first day of the storage period. Moreover, the HIT process reduced the diameter of the fat globules in the goat milk, making them smaller than those of homogenized milk. This pretreatment could be used successfully in the production of stirred yoghurt to improve major quality parameters such as delayed syneresis, increased viscosity and enhanced sensory properties during storage.

Pengaruh Fraksi Minyak dan Emulsifier serta Kecepatan Pengadukan terhadap Karakteristik Emulsi Minyak Biji Bunga Matahari (Helianthus annuus L.) dalam Air (M/A)

The O/W emulsion of sunflower seed oil base cream has the advantage of maintaining skin moisture, anti-inflammatory, easy to apply, not sticky, and easily washed off with water. A Formula has an important role in a base cream manufacturing due to improper formula causing drastic changes in the properties and characteristics. These experiments were studied to investigate the effects of fraction of natural emulsifier, fraction of sunflower seed oil, and impeller speed on the base cream characteristics. The characteristics observed were spreadability, globule diameter, and stability of the base cream. The base cream was prepared in some formulas and various of impeller speeds when the oil phase was poured into the water phase gradually. The working temperature was kept between 60-70&lt;sup&gt;o&lt;/sup&gt;C for 30 minutes of mixing. Observations of the spreadability and the globule diameter were conducted every 5 minutes, while the stability just observed at the end of process. The treatment of 2000 rpm of impeller speed on 4% of emulsifier and 10% of sunflower seed oil resulted in the base cream with spreadability of 7.1 &lt;span style="text-decoration: underline;"&gt;+&lt;/span&gt; 0.68 cm and globule diameter of 12.7 &lt;span style="text-decoration: underline;"&gt;+&lt;/span&gt; 1.62 µm, while all of the formulas and impeller speeds resulted in O/W base cream having good stability.

Influence of coordination environment and dielectric contrast on the spectral-luminescent properties of europium ions in the pores of film opals

The photoluminescence spectra of the inorganic salt of europium C6H9EuO6 × H2O in the pores of film opals, with globule diameter D ≈ 300 nm on a glass substrate, were measured. In the measured spectra the radiative transitions in the energy spectrum of Eu3+ 5D0→7F0, 5D0→7F1, and 5D0→7F2 were observed at wavelength 580 nm, 592 nm and 617 nm, respectively. The presence in the luminescence spectra of the maximum possible number of components of the Stark structure of the 5D0→7FJ (J=1–2) transitions, equal to 2J+1, and the high intensity of the 5D0 → 7F0 transition indicate that the symmetry of the luminescence centers is low, not higher than C3. The dependence of the luminescence spectra on the additional impregnation of the samples with glycerol was established: smoothing of the Stark structure and broadening of the 5D0→7F2 band were observed, as well as a decrease in the ratio of integrated intensities I(5D0→ 7F2 ) / I(5D0 →7F1) from 3.6 to 1.9.

Spray atomisation in multiphase flows with reference to tank mixes of agricultural products

First-principle models describing the breakup of liquid sheets into droplets are used to screen spray adjuvants and agricultural tank mix partners to eliminate combinations that might negatively impact droplet size, reducing the need for extensive experimental testing. An agent-based model is developed to predict droplet spectra following spray atomisation from a flat-fan nozzle for a continuous oil-in-water liquid sheet that comprises the mechanisms of hole nucleation, hole growth, hole coalescence, ligament formation and rupture into atomised drops. Hole nucleation is driven by Marangoni spreading of oil globules at the interface that initiates sheet breakup, followed ultimately by fluid ligament rupture into droplets of sizes conforming to a gamma distribution. This mechanistic model is used to predict the effect of various relevant parameters on the volume fraction of driftable fines (DFV, fine spray that can easily become entrained in wind, typically <150 μm diameter) resulting from spray atomisation. An increase in the concentration of the emulsion immiscible phase increases the rate of hole nucleation in the spray sheet and decreases the DFV. In addition, increasing the surface tension of the emulsion aqueous phase is also found to substantially decrease the DFV. A small decrease in DFV is observed with increase in the mean oil globule diameter, and negligible change is observed when varying the viscosity by 10× over water. Experimental droplet spectra from commercially available formulations mixed together are used to validate this numerical approach for mixture performance with excellent agreement.

Water Dynamics in Aqueous Poly-N-Isopropylacrylamide Below and Through the Lower Critical Solution Temperature.

Poly-N-isopropylacrylamide (PNIPAM) is a thermo-responsive polymer that exhibits a reversible structural change from extended chains to aggregates in aqueous solution above its lower critical solution temperature (LCST). Using polarization-selective IR pump-probe spectroscopy, the water orientational dynamics in PNIPAM from below to above the LCST were examined and compared to those of its monomer solution, N-isopropylacrylamide (NIPAM), polyacrylamide, and an acrylamide monomer solution, which are not thermo-responsive. The OD stretch of dilute HOD in H2O is used as a vibrational probe of water orientational dynamics. Below the LCST of the polymer, NIPAM and PNIPAM solutions exhibited identical water dynamics that were significantly different from those of bulk water, containing both faster and slower components due to solute-water interactions. Therefore, there is no difference in the nature of water interactions with a single NIPAM moiety and a long polymer chain. For all systems, including PNIPAM below and above the LCST, the orientational dynamics were modeled with a bulk water component and a polymer/monomer-associated component based on previous experimental and computational findings. Above the LCST, PNIPAM showed fast water orientational relaxation but much slower long-time dynamics compared to those of NIPAM. The slow component in PNIPAM, which was too slow to be accurately measured due to the limited OD vibrational lifetime, is ascribed to water confined in small voids (<2 nm in diameter) of PNIPAM globules. These results highlight important details about thermo-responsive polymers and the dynamics of their solvation water as they undergo a significant structural change.

The effect of acclimation temperature and optimal temperature gradient for egg and larvae of silver moony (Monodactylus argenteus) during the early ontogenesis.

The early life history of a fish species is regulated by temperature, the most critical environmental cue. Thus, identifying the gradient of temperature that optimises the early development of a species is a prerequisite for standardising hatchery technology. Silver moony, Monodactylus argenteus is a tropical brackishwater ornamental fish that holds scope for the Indian and Global ornamental fish industry. This study unravels the effect of water temperature increments (26, 28, 30, 32, and 34°C) on embryonic development, hatching, and survival rate, as well as the growth profile and survival rate of larvae at 5days post-hatch (5 dph). Experiments were conducted to find out the optimal temperature gradient for egg incubation and early larval rearing. The experiment results revealed that the embryogenesis was accelerated at increasing temperatures, especially after the gastrula stage, and apparent differences were evident in each stage. However, the morphological development profile at each embryonic stage was similar throughout the temperature range. The incubation period differed significantly (P < 0.05) between the temperature gradients. The highest rates of hatching (90-100%) and survival after 12 hph were observed at 28 ºC and 30 ºC. Hatched-out larvae demonstrated the highest total length (1.92 ± 0.02µm) at 34°C, and the total length decreased at lower temperature levels. The yolk sac volume of larvae was shrunken with an increase in temperature, and a significant difference was observed between the studied temperatures. However, the oil globule diameter did not differ between the different temperatures. The total length and growth rate of 5 dph larvae were significantly different among the temperature treatments and increased with increasing temperature. In contrast, the survival rate of 5 dph larvae was highest at the range of 26 ºC and 30 ºC. The results indicated that the change in temperature from the spawning temperature (29 ± 1°C) negatively influenced embryogenesis and the early development of M. argenteus. Based on the experimental results, the growth and survival of embryo and larvae were found to be optimum at 28 to 30 ºC. This prediction is of great importance for the effective management in the hatchery production phase, and especially the temperature could be considered the critical environmental cue.

Glycerin diet affects the size of the fat globule and the fatty acid profile of goat's milk

The objective of this study was to evaluate the production, fat and fatty acids of milk as well as the diameter, classification and quantity of the milk fat globules (MFG) of goats consuming a diet with 15% of crude glycerin. Twelve multiparous Saanen goats weighing 40 ± 6 kg and 90 ± 5 days of lactation were used. The experimental design was completely randomized with two treatments (0 and 15% inclusion of glycerin). In this way, each milk sample is classified according to the percentage of milk fat globules that were included in these three size categories. For the variables milk production, fat, diameter, medium and large fat globules and amount of globules showed a significant effect of the inclusion of glycerin in the diet (p < 0.05). Fifteen fatty acids were found, mostly saturated. For caprylic, palmitic, and linoleic fatty acids, there was no significant influence of the inclusion of glycerin (p > 0.05). Can recommend the use of 15% double-distilled glycerin in the feeding of lactating goats increased milk production, the amount of fat, increased the size of the milk fat globule. Regarding the saturated and polyunsaturated fatty acids, they kept the level of 15% of glycerin.

Aktivitas Antioksidan dan Evaluasi Fisik Sediaan Krim Tabir Surya dari Bubur Rumput Laut Turbinaria conoides dan Serbuk Daun Kelor (Moringa oleifera)

Moringa and brown seaweed (T. conoides) are known as natural ingredients that are good for the human body. One of them was a good bioactive component as a cosmetic. Research on the combination of these two ingredients has never been done. The aims of this study were to determine the best sunscreen cream from the combination of Moringa leaves and T conoides. In this study, several stages were carried out, namely the production of seaweed porridge and Moringa leaf powder, and formulation of cream preparations with the ratio treatment of T. conoides. and Moringa, namely 1:1 (S), 1:2 (T), 2:1 (U) and control/without treatment (R). The tests included antioxidant and evaluation of physical parameters of sunscreen cream preparations. Evaluation of physical parameters included centrifugal test, emulsion type, globule diameter, and organoleptic. Antioxidant activity test showed that cream S was significantly different (p&lt;0.005) from cream T and cream U, and it was strong antioxidant activity. The centrifugal test for all treatments showed that all cream preparations were stable because there was no phase separation. The type of emulsion in all treatments was O/W. The diameter of the globules in all treatments had sizes that were still within the allowable range. The organoleptic results on the color and aroma parameters of each cream were not significantly different (p&lt;0.005) in all treatments. These results are different from the appearance parameters because cream S and T are significantly different (p&lt;0.005). Cream S is the best cream in this study from the antioxidant activity and all evaluation parameters.

Influence of low molecular weight surfactants on the stability of model infant formula emulsions based on hydrolyzed rice protein

The emulsifying properties of rice protein ingredients, and their behaviour in the presence of other non-protein emulsifiers, are essential to support food applications. The objective of this study was to investigate the influence of different low molecular weight surfactants (LMWS) on the stability of model infant formula emulsions (10 g protein L−1, 35 g soybean oil L−1, 50 g carbohydrate L−1) based on a rice protein hydrolyzate (RPH). Inclusion of CITREM and DATEM, at concentrations greater than 1 g L−1, gave emulsions with mean fat globule diameters (D[4,3]) less than 1 μm with good creaming stability, while lecithin was less effective across the concentration range tested (i.e., 1–3 g L−1). Coalescence of the control emulsion was observed upon both storage (10 d at 4 °C) and heat treatment (95 °C × 5 min). Increasing CITREM concentration increased storage and heat stability of the emulsions, while addition of CITREM at 2 g L−1 facilitated the formation of a very stable product. This study provides a first insight into the potential of hydrolyzed rice protein to be used as a value-added ingredient in the production of nutritional beverages such as infant formula emulsions, and the influence of LMWS on the stability of such products.

The Effect of Cold Press Chia Seed Oil By-Products on the Rheological, Microstructural, Thermal, and Sensory Properties of Low-Fat Ice Cream.

This study aimed to investigate the utilization of cold-pressed chia-seed oil by-products (CSOB) in a low-fat ice cream formulation as a fat replacer and stabilizer. In the study, ice cream emulsion mixtures were formulated by using 0.2–0.4% xanthan gum (XG), 2.5–12.5% fat, and 1–3% CSOB. Optimization was performed using the response surface methodology (RSM) and full factorial central composite design (CCD) based on the flow behavior rheological properties of the emulsions obtained from 17 different experimental points. All of the emulsion samples showed non-Newtonian shear-thinning flow behavior. The consistency coefficient (Κ) values of the emulsion samples were found to be 4.01–26.05 Pasn and were significantly affected by optimization parameters (p < 0.05). The optimum formulation was determined as 0.29% XG, 2.5% CSOB, 2.5% fat. The low-fat (LF-IC) and full-fat control samples (FF-IC) were compared to samples produced with an optimum formulation (CBLF-IC) based on the steady shear, frequency sweep, and 3-ITT (three interval thixotropy test) rheological properties, thermal properties, emulsion stability, light microscope images, and sensory quality. CBLF-IC showed similar rheological behavior to FF-IC. The mix of CBLF-IC showed higher emulsion stability and lower poly-dispersity index (PDI) value and fat globule diameters than those of FF-IC and LF-IC. The thermal properties of the samples were significantly affected by the addition of CSOB in an ice cream mix. CBLF-IC exhibited a lower temperature range (ΔT), enthalpy of fusion (ΔHf), and freezing point temperature (Tf) than those of FF-IC and LF-IC. While CBLF-IC exhibited a higher overrun value than other samples, it showed similar sensory properties to the FF-IC sample. The results of this study suggested that CSOB could be used successfully in low-fat ice cream production. This study also has the potential to gain new perspectives for the evaluation of CSOB as a fat substitute in a low-fat ice cream.

Magnetic Fractions of PM2.5, PM2.5–10, and PM10 from Coal Fly Ash as Environmental Pollutants

Characterization of magnetic particulate matter (PM) in coal fly ashes is critical to assessing the health risks associated with industrial coal combustion and for future applications of fine fractions that will minimize solid waste pollution. In this study, magnetic narrow fractions of fine ferrospheres related to environmentally hazardous PM2.5, PM2.5–10, and PM10 were for the first time separated from fly ash produced during combustion of Ekibastuz coal. It was determined that the average diameter of globules in narrow fractions is 1, 2, 3, and 7 μm. The major components of chemical composition are Fe2O3 (57–60) wt %, SiO2 (25–28 wt %), and Al2O3 (10–12 wt %). The phase composition is represented by crystalline phases, including ferrospinel, α-Fe2O3, ε-Fe2O3, mullite, and quartz, as well as the amorphous glass phase. Mössbauer spectroscopy and magnetic measurements confirmed the formation of nanoscale particles of ε-Fe2O3. Stabilization of the ε-Fe2O3 metastable phase, with quite ideal distribution of iron cations, occurs in the glass matrix due to the rapid cooling of fine globules during their formation from mineral components of coal.

Impact of the encapsulated salt characteristics on its release from multiple W/O/W emulsions

AbstractW/O/W emulsions based on Miglyol oil and stabilized by polyglycerol polyricinoleate and sodium caseinate were prepared with similar characteristics in terms of internal droplet diameter, oil globule diameter, internal aqueous fraction and fat globules fraction. The emulsions differ only by the nature of the encapsulated salt in the inner aqueous droplets. The release of each encapsulated salt from the internal to the external aqueous phase was followed during 30 days at 25°C, showing for all cases that the double structure was preserved with time and the release increased during storage, mainly according to diffusion/permeation mechanism and resulting in a competition between the chelation and the hydration enthalpy of the salt. Double emulsions encapsulating different Magnesium salts have shown that the release depends strongly on the anion via its magnesium chelation and its hydration enthalpy. Indeed, as the complexation constant of Mg2+ with its counterion increases, the leakage decreases. On the other hand, as the hydration enthalpy of Mg2+ counterion decreases, the leakage decreases too. Likewise, double emulsions encapsulating different chloride salts have shown that the release depends also on the cation via its hydration enthalpy and its chelation with caseinate affecting accordingly the stiffening of the globule/water interface, more pronounced for bivalent than for monovalent cations. It was thus directly highlighted that the release from W/O/W emulsions was well controlled by the characteristics of the salt, and anion and cation diffuse accompanied at a rate ensuring the electroneutrality of the diffusive species. These results are very useful not only in term of fundamental understanding but also on application level, especially for food supplementation such as of dairy product.Practical applicationsThe search for an innovative functional effect can lead to food supplementation with bioactive substances which can be either molecules or living bioactive cells. However, the direct addition of these substances to food can induce chemical degradation, aggregation of proteins and generate an unpleasant taste. These drawbacks can be avoided or at least reduced by encapsulation. Despite their thermodynamic instability, W/O/W emulsions are promising systems for encapsulation of hydrophilic species in their internal aqueous phase and hence find applications among others in food for taste masking or supplementation. The release of the encapsulated species to the external aqueous phase should be controlled, depending on the desired application. The release depends also on the characteristics of the encapsulated salt. A better understanding of its impact on the stability of the double emulsion will allow targeting formulation of stable emulsions and hence contributing to their better industrial development.