Pantala Flavescens Research Articles

Dragonfly Community in Flowing and Stagnating Water in the Cibodas Botanical Garden Area

There are about 5000-6000 types of dragonflies which will continue to grow when new types are found. The diversity of dragonflies in Indonesia is quite high, namely about 750 species or 12.5% of the total in the world. This study aims to determine the dragonfly community in flowing and stagnant water in the Cibodas Botanical Garden Area. The research method used was a descriptive exploratory method, while the data collection technique used the Catch and Release technique (TLK). Data analysis in this study used descriptive analysis. Data collection was carried out in May-July 2018. The locations included in the flowing water group were Sakura Park and Ciismun Waterfall, while Air Mancur and Guest House were included in the stagnant water group. The results showed that there were 8 types of dragonflies identified, namely Orthetrum Pruinosum, Orthetrum sabina, Orthetrum glaucum, Pantala flavescens, Neurothermis fluctuans, Neurothemis terminata, Ischnura senegalensis, Coeliccia membranipes. The most common species found was Pantala flavescens with 533 individuals, while the least species found was Neurothemis terminata with 4 individuals. Sub-order zygoptera was found only in 2 types in this study, namely the Coeliccia membranipes with the number of 101 individuals and Ischnura senegalensis with the number of 19 individuals. The dominant types of dragonflies in flowing and stagnant water are Pantala flavescens and Orthetrum pruinosum. The Coeliccia membranipes dragonfly was only found in running water, namely at the location of the Ciismun waterfall and Ischnura senegalensis only found in stagnant water, namely at the location of the fountain.

Corrigendum to: Long-Distance Migration of the Globe Skimmer Dragonfly to Japan Revealed Using Stable Hydrogen (δ 2H) Isotopes.

Corrigendum to: Long-Distance Migration of the Globe Skimmer Dragonfly to Japan Revealed Using Stable Hydrogen (δ 2H) Isotopes.

The first complete mitochondrial genome of the migratory dragonfly Pantala flavescens Fabricius, 1798 (Libellulidae: Odonata)

Pantala flavescens is the world’s most abundant and widely distributed dragonfly and with its outstanding migratory capacity an important model system to study insect migration at the evolutionary base of winged insects. We here report on the first complete mitochondrial genome (mitogenome) of P. flavescens sampled from a population in Rufiji River, Tanzania. The mitogenome is 14,853 bp long with an AT-biased base composition (72.7% A + T) and encodes a typical set of 13 protein-coding genes (PCGs), 22 tRNAs, and two rRNAs. The control region (CR) (171 bp) is the shortest reported in any anisopteran odonate, so far. Phylogenetic analyses support the placement of P. flavescens within the Libellulidae.

Seasonal Migrations of Pantala flavescens (Odonata: Libellulidae) in Middle Asia and Understanding of the Migration Model in the Afro-Asian Region Using Stable Isotopes of Hydrogen.

Simple SummaryLarge-distance migrations of insects have been recognized for many years, but many details of this behaviour remain unknown. The globe skimmer dragonfly has the most extensive cosmopolitan range among all dragonfly species. Migrations of these dragonflies are noted on all continents (except Antarctica), over both land and the oceans, but the patterns of their seasonal movements are still poorly understood. We aimed to confirm seasonal latitudinal migrations of the globe skimmer in Middle Asia and to clarify its migration pattern in extended areas. We used stable isotope composition of hydrogen in wings of dragonflies as an intrinsic marker of their places of origin. Combining phenological data and a comparison with published isotopic data on migratory insects, our results suggest that in spring, the already-mature dragonflies arrive in Middle Asia for reproduction from tropical parts of East Africa and/or the Arabian Peninsula, and, in autumn, summer-generation dragonflies migrate to the south. We conclude that in the Afro-Asian region there is an extensive migration circle of the globe skimmer covering East Africa, Central Asia and the Indian subcontinent with a total length of more than 14,000 km.In Middle Asia, the dragonfly Pantala flavescens makes regular seasonal migrations. In spring, sexually mature dragonflies (immigrants) arrive in this region for reproduction. Dragonflies of the aboriginal generation (residents) develop in about two months, and migrate south in autumn. Residents of Middle Asia have significantly lower δ2H values (−123.5 (SD 17.2)‰, n = 53) than immigrants (−64.4 (9.7)‰, n = 12), as well as aboriginal dragonfly species from Ethiopia (−47.9 (10.8)‰, n = 4) and the Sahel zone (−50.1 (15.5)‰, n = 11). Phenological data on P. flavescens in the Afro-Asian region and a comparison with published isotopic data on migratory insects from this region suggest that (i) the probable area of origin of P. flavescens immigrants is located in tropical parts of East Africa and/or the Arabian Peninsula and (ii) the autumn migration of Middle Asian residents to the south may also pass through the Indian Ocean. We assume that in the Afro-Asian region, there is an extensive migration circle of P. flavescens covering East Africa, Central Asia and the Indian subcontinent with a total length of more than 14,000 km.

Diversity and Abundance of Insect in Conventional Apple (Malus sylvestris (L.) Mill) Plantation at Kota Batu, East Java

Apple is a plant that susceptible toward pests and diseases. Application of pesticide to suppress insect pest population gave negative impact toward natural enemies and insect pollinators. The purpose of this research was to determine the diversity and dominance of insect pests, pollinators, and natural enemies of each phase of apple plant development in conventional plantations in Kota Batu, East Java. This research was conducted in February to May 2016 in each phase of apple growth namely, after defoliation, early flower, late flower, early fruit, and late fruit. Plot size was 10x10 m2 with total 5 plots and total plants in each plot were 60 trees. Collection methods were active collection (hand picking, insect net, and beating tray) and passive collection (yellow trap, pitfall trap, light trap, and stainer trap). Preservation methods used in this research were dry preservatoin and wet preservation. Identification was conducted in Laboratorium of Entomology, Faculty of Biology UGM and Laboratorium Entomology, Zoology Division, Research Centre for Biology Indonesian Institute of Sciences, Cibinong. Data analyzed by using Shannon-Wiener Diversity Index, Simpson Dominance Index and Abundance Formulation. The results showed that total insects that was caught consist of 38 species belong to 9 orders. Diversity index in each phase of apple growth were: after defoliation (0.69), early flower (1.39), late flower (1.86), early fruit (0.66), and late fruit (1.24). Domination index each phase of apple growth were after defoliation (0.50), early flower (0.34), late flower (0.21), early fruit (0.75), and late fruit (0.40). Diversity index of potential insect as pest (1.46), as pollinator (1.29), and as natural enemies (1.18). The highest abundance of insect as pollinator was Apis cerana, as natural enemy was Pantala flavescens, and as pest was Aphis gossypii.

Long-Distance Migration of the Globe Skimmer Dragonfly to Japan Revealed Using Stable Hydrogen (δ 2H) Isotopes.

The globe skimmer dragonfly, Pantala flavescens Fabricius (Odonata: Libellulidae), is a long-distance migrant, well adapted to exploiting ephemeral waterbodies. This species occurs in Japan every summer, but overwintering has only been recorded on subtropical Ishigaki Island. It is not known from where the summer immigrants originate, nor what proportion of the globe skimmers seen in Japan are of local origin. We analyzed stable hydrogen isotope (δ 2H) composition of wings of 189 P. flavescens captured at six sites in Japan from August to September in 2016 (n = 57) and from April to November in 2017 (n = 132). We determined that the majority of individuals were immigrants. Individuals of probable Japanese origin occurred only later in the year and were of lower mass on average than immigrants. Immigrants potentially originated from a broad area as far west as northern India and the Tibetan Plateau and, especially late in the season, as near as northcentral China and the Korean peninsula. However, for April samples, the most parsimonious interpretation suggested southern origins, in northern Myanmar to southern China, or possibly Borneo-Sulawesi. Our investigation underlines the power of combining stable isotope data with other information such as wind speed and direction, arrival dates, and body mass to estimate origins and to understand the life history of this and other insects.

Dragonfly-Associated Trichoderma harzianum QTYC77 Is Not Only a Potential Biological Control Agent of Fusarium oxysporum f. sp. cucumerinum But Also a Source of New Antibacterial Agents.

A strain isolated from the gut of Pantala flavescens was characterized as Trichoderma harzianum QTYC77. The strain was assessed as a potential biocontrol agent against Fusarium oxysporum f. sp. cucumerinum (FOC). Mycoparasitism and competing abilities of T. harzianum QTYC77 lead to inhibition of the mycelial growth of FOC, with the inhibition rate of 70.99%, in dual culture assays. Activities of chitinase and β-1,3-glucanase, responsible for fungal cell-wall degradation, were gradually increased and their activities were the maximum on the fifth day of fermentation with 23.20 and 1.84 U/mL, respectively. T. harzianum QTYC77 was discovered to have potent biocontrol potential with the control efficiency of 67.43% against the FOC in vivo pot experiment. Furthermore, two novel compounds azaphilone D (1) and E (2) along with three known metabolites 3-hydroxymethyl-6, 8-dimethoxycoumarin (3), harzianone (4), and pachybasin (5) were isolated and identified from T. harzianum QTYC77. Unfortunately, these metabolites did not show antifungal activities against FOC. However, both metabolites 1 and 3 displayed moderate activity against Staphylococcus aureus with disc diameters of zone of inhibition (ZOI) of 7.3 and 7.2 mm, respectively, compared with that of referenced gentamycin (ZOI = 14.5 mm). In addition, metabolite 1 possessed a moderate antibacterial activity against Bacillus subtilis with a ZOI value of 7.0 mm compared with that of positive gentamycin (ZOI = 15.2 mm). The present results suggested that T. harzianum QTYC77 was not only a potential biofungicide against FOC but also the source of new antibacterial agents.

Dragonflies and damselflies (Odonata) from Flores Island, Lesser Sunda Archipelago: New occurrences in extreme environments and an island-level checklist of this group

Although the Odonata are common inhabitants of various extreme environments such as geothermal springs, brackish wetlands, mangroves, and volcanic lakes, the assemblages of this group associated with extreme habitats in Australasia are rather poorly known. Here, we combine museum collection data and published reports on Odonata from extreme habitats on Flores Island, Lesser Sunda Archipelago. The highly acidic Sano Nggoang Crater Lake (mean pH = 3.17) on Flores houses seven species as follows: Agriocnemis pygmaea, Xiphiagrion cyanomelas (Coenagrionidae), Neurothemis ramburii, Orthetrum pruinosum pruinosum, O. sabina, O. testaceum soembanum (Libellulidae), and Anax gibbosulus (Aeshnidae). A coastal marsh site with slightly brackish water on Flores harbors at least five dragonfly species as follows: Diplacodes trivialis, Neurothemis intermedia excelsa, N. terminata, Pantala flavescens, and Rhyothemis phyllis ixias (Libellulidae). The migratory dragonfly Pantala flavescens was a single species recorded on the waterless Kanawa Island near the western edge of Flores. Our findings suggest that extreme habitats in eastern Indonesia primarily colonized by widespread generalist Odonata species. Finally, an updated checklist of Odonata species recorded from Flores Island was compiled. Our survey of museum specimens recovered two species not found on existing species lists for Flores: Neurothemis intermedia excelsa and Pantala flavescens.

Dragonfly Diversity (Insect: Odonata) in Asem Binatur River, Pekalongan, Indonesia

Dragonflies have an essential role in the aquatic ecosystem. The nymph phase lives in water and is sensitive to environmental changes. The reduced number of dragonflies in an area can be an indication of changes in the quality of water and environmental health. This research was conducted in the Asem Binatur River, Pekalongan, Indonesia, from November 2018 to April 2019. The purpose of this study was to determine species diversity, the index value of the Pekalongan River dragonfly diversity by using the point count method. Long hand high net, wrapping paper/papilot and camera were used to catch and observe the dragonflies. The Shannon-Wiener species diversity index was calculated. Seven species of dragonflies were recorded from Asem River. There were Orthetrum sabina, Diplacodes trivalis, Neurothemis ramburii, Crocothemis servilia, Pantala flavescens, Neurothemis stigmatizans and Orthetrum chrysis. The Shannon-Wiener diversity index analysis was ranged from 0 to 0.31, indicating low diversity. Abiotic parameter measurements showed the humidity was 67.17%, the soil pH was 7.11, the light intensity was 46310 lux and the temperature was 30 °C. The analysis of the dragonfly diversity index was correlated with the poor water quality status in Asem Binatur River, Pekalongan.

Behavioral responses of tadpoles of Duttaphrynus melanostictus (Anura: Bufonidae) to cues of starved and fed dragonfly larvae

Behavioral responses of tadpoles of Duttaphrynus melanostictus (Anura: Bufonidae) to cues of starved and fed dragonfly larvae. Tadpoles of Duttaphrynus melanostictus use chemoreception to detect kairomonal cues and excretory metabolites from predatory anuran tadpoles (Hoplobatrachus tigerinus) that consume them. We describe here the behavioral responses of tadpoles of D. melanostictus to predatory dragonfly larvae (Pantala flavescens). The predator’s kairomones (water conditioned by the starved predator) or its diet-derived metabolites released in excreta of predator after consumption of conspecific prey tadpoles were used to simulate predation risk. The tadpoles of D. melanostictus had no behavioral response to predator kairomones. However, the larvae reduced swimming movements and overall time spent in swimming, and had a higher burst speed/swimming velocity in response to water borne cues released from the excreta of predators fed conspecific prey. Thus, just the presence of dragonfly larvae does not elicit defense behaviors in tadpoles of D. melanostictus, but when predation risk is recognized as real (i.e., when tadpoles are exposed to excretory metabolites of predators fed conspecific tadpoles), defense behaviors are activated.

Aerodynamic and Experimental Analysis of Bio-mimic corrugated dragonfly aerofoil

In this work, experimental and computational approach is used to understand the corrugation attitude of a bio-inspired dragonfly mimicked corrugated airfoil at low Reynolds number varying from 15000 to 75000 to understand the advantages of pleated corrugated airfoil. The CFD analysis is carried out on the 2-dimensional bio-mimetic corrugated ‘Pantala flavescens’ dragonfly forewing to predict the aerodynamic characteristics of the corrugated dragonfly aerofoil with varying angle of attack from 0° to 8°. The computational analysis of the wing profile is done using the ANSYS-19 ICEM CFD and FLUENT software. For the experimental test, the model is printed in 3-D printer machine and tested in subsonic Wind Tunnel at different speeds and different angle of attacks using a wind tunnel 6-component balance. The computational simulation reveals the exemplary results of the pleated airfoil (corrugated aerofoil) with new design constraints. Finally, the computational result is validated with experimental results.

Survival strategy of Pseudomonas aeruginosa on the nanopillar topography of dragonfly (Pantala flavescens) wing

Discovery of nanopillars on the surface of the insect wings had led to the understanding of its bactericidal property. Nanopillar topography is deterrent to only those bacteria that are attached, or in close contact with the nanopillars. The present study investigated the variation in the viability of Pseudomonas aeruginosa strains PAO1 (virulent) and ATCC 9027 (avirulent) on the wing surface of dragonfly (Pantala flavescens). Viability study indicated that only 0.2% ATCC 9027 survived when incubated with wing for 48 h in Phosphate buffered saline, while under the same conditions 43.47% PAO1 survived. Enumeration of Pseudomonas attached to wing surface suggested that, the number of PAO1 attached on the wing surface was three times lesser than ATCC 9027. Propensity of attachment of P. aeruginosa strains PAO1 and ATCC 9027 on the wing surface investigated using scanning probe microscope indicated that P. aeruginosa ATCC 9027 showed adhesion to 88% of regions and, PAO1 showed adhesion to only 48% regions tested on wing surface. PAO1 survived the bactericidal effect of wing surface by evading attachment. Three clinical isolates tested which showed viability similar to PAO1 strain, also showed lower propensity to attach to wing surface. Transcriptional level analyses using RT-PCR suggested that flagellar genes (fliE and fleS) were downregulated and genes responsible for reversible to irreversible attachment (gcbA and rsmZ) were upregulated in ATCC 9027 than PAO1 on wing surface, indicating relatively higher attachment of ATCC 9027 on wing surface. The study suggests that virulent strains of P. aeruginosa may evade attachment on wing surface. The results gain significance as bioinspired surfaces are being created towards developing antibacterial medical implants and other antibacterial surface applications.

Modal and Harmonic Analysis of Bio-Mimic Corrugated Dragonfly Aerofoil

In this present work, free vibration analysis is carried out on the 2-dimensional bio-mimetic corrugated ‘Pantala flavescens’ dragonfly forewing. The numerical modeling based on Finite Element Method (FEM) is developed to predict structural limitation of the dragonfly wings Dragonfly wings bend and twist over the course of flight stroke, causing deformation by a combination of elastic and inertial forces. We found that the wing deformation is smaller during the downstroke and it is revealed that the structural maximum beat frequency of a corrugated dragonfly wing is 38.34 Hz during hovering flight. The modal and harmonic analysis is performed using ANSYS 15 Mechanical APDL to extract the wing’s natural frequency and mode shape of corrugated wing which is significant in design, analysis and manufacturing of analogous MAVs structure.

Short Communication: Community structure and diversity of Odonata in Suranadi Natural Park, West Lombok Indonesia

Abstract. Ilhamdi ML, Al Idrus A, Santoso D, Hadiprayitno G. 2020. Short Communication: Community structure and diversity of Odonata in Suranadi Natural Park, West Lombok Indonesia. Biodiversitas 21: 718-723. Odonata is an order of insects. The life cycle of this order is dependent on freshwater habitat. Sensitivity to environmental changes causes odonates to be important bioindicators of ecosystem change. The importance of the ecological role of odonates is not followed by adequate scientific information on Odonata communities, especially in the Suranadi Natural Park (SNP). The study aimed to monitor the existence and community structure of Odonata in the SNP area every six months in period. The data collection technique was using a survey method by following the transect line, namely the waterway transect line (WTL), plantation border transect (PT) and settlement border transect (ST). Data collection was done 4 times from April to May 2019, once every 2 weeks. The sampling was done twice in the morning from 08.00 to 11.00 AM and in the afternoon from 03.00 to 05.00 PM. Data were analyzed using the relative frequency equation and Diversity Index. The results showed that there were 16 species of odonates consisting of the family Libellulidae (11 species), Platycnemididae (1 species), Chlorocyphidae (1 species), and Coegrinidae (3 species). Orthetrum sabina, Neurothemis ramburii, Diplacodes trivialis, Gynacantha subinterrupta, Copera marginipes, and Pantala flavescens, are species that are present in all of each observation sites. The waterway transect has the highest diversity index (2.027), followed by settlement transect (1.367), and the lowest is plantation transect (1.131).

Long-term shifts in abundance of (migratory) crop-feeding and beneficial insect species in northeastern Asia

Vast numbers of insects annually engage in trans-latitudinal migration and thereby impact structure and functioning of natural and man-made ecosystems. In eastern Asia, long-distance migration has historically been studied for single insect species rather than diverse species complexes. Here, we assessed migration dynamics of multiple economically important migratory species on an island in the Bohai Strait, China. Drawing upon 15-year trapping records of > 2.5 million specimens, we unveil inter- and intra-annual shifts in the species composition and abundance of migrant individuals. Migrants belonged to 9 orders and 36 families, primarily consisting of Lepidoptera (79% individuals), Odonata (8%), and Coleoptera (4%). Seven crop-feeding noctuids, e.g., Helicoverpa armigera (Hübner), Mythimna separata (Walker), represented 54% of the total trapping records. Trap catches exhibited marked seasonal variation, with the highest capture rate during early fall. Yearly abundance of migratory noctuids was coupled with that of their associated natural enemies. Although overall trap catches did not decrease over the monitoring period, the entire order of Odonata experienced a 14.1% annual rate of decline. Furthermore, 19 out of 108 species exhibited a progressively declining abundance over time, including the cosmopolitan cutworm Agrotis ipsilon (Hufnagel) and the insectivorous dragonfly Pantala flavescens Fabricius. Our work provides unprecedented insights into insect migration dynamics in eastern Asia, helps fine-tune forecasting and early-warning systems of crop pests, and thereby guides integrated pest management within local agro-landscapes. Also, a long-term tracking of migrant insect populations can shine light on the fate of (insect-mediated) ecosystem services and trophic dynamic processes at a macroscale.

Richness and diversity of odonates of the agricultural college and research institute, Vazhavachanur, Tamilnadu, India

Investigations on the diversity of Odonata in and around the Agricultural College and Research Institute, Vazhavachanur, Tamil Nadu, India were studied. Eight locations were selected, of which sixteen Odonata species were recorded. In total, eleven dragonfly and five damselfly species were identified from Thiruvannamalai district, Tamil Nadu, India. Pantala flavescens, Diplacodes trivialis, Brachythemis contaminata and Ischnura aurora were recorded from all eight locations. Trithemis pallidinervis and Agriocnemis pygmaea were recorded from seven locations except from the farm pond and the open stretch area. Rhyothemis variegata was recorded only at the open stretch area. The results clearly show that, Odonates have specific habitat preferences for their growth and development. Four families Libellulidae, Gomphidae, Aeshnidae and Coenagrionidae were observed and collected during the study. Libellulidae were the most abundant family (56.25%) and comprised of 9 species, followed by Coenagrionidae (31.25%) with 5 species. Low species abundance was recorded within the Gomphidae and Aeshnidae families with 6.25% each. Simpson and Shannon Index showed maximum diversity in the open stretch area (0.8743 and 2.186, respectively) and minimum in location 8 (crop production area) and location 4 (Sathakuppam), respectively. Open stretch area showed maximum Richness Index (Margalef) (2.275) followed by farm pond (2.196) where recorded minimum in sathakuppam (1.276) location. Equitability J index, maximum species evenness (0.788) was recorded Location 6 i.e. farm pond (0.6727) followed by open stretch area 0.686.

Distribution and migration strategy of Pantala flavescens (Fabricius, 1798) (Odonata, Libellulidae) near the northern limit of the range in Transbaikalia and in the Far East of Russia

Distribution and migration strategy of Pantala flavescens (Fabricius, 1798) (Odonata, Libellulidae) near the northern limit of the range in Transbaikalia and in the Far East of Russia

Odonata Diversity at Sumber Clangap and Sumber Mangli Puncu Village Sub District of Puncu District of Kediri

Sumber Clangap and Sumber Mangli are geographically located at the Mount Kelud steeps. Administratively located at Puncu Village, Puncu Sub-district and District of Kediri. They provided hab-itat for the flora than fauna especially Odonata that never been stud-ied before. We aimed to study Odonata diversity at Sumber Clangap and Sumber Mangli area. The method used in this study was natural snapshot experiment that conducted by Odonata monitoring. Micro-climate parameter including air temperature and humidity were not-ed. Odonata activity and behavior noted for analysis. Collected data were analyzed using Shannon-Wiener heterogeneity index. The results showed that there were 17 species from the whole location. There was Euphaea variegata, Vestalis luctuosa, Rhinocypha anisoptera, Peric-nemis stictica, Pseudagrion pruinosum, Coeliccia membranipes, Gy-nacantha subinterrupta, Idionyx montana, Paragomphus reinwardtii, Heliogomphus drescheri, Neurothemis fluctuans, Orthetrum glau-cum, Orthetrum pruinosum, Orthetrum sabina, Pantala flavescens, Trithemis festiva and Zygonyx ida. Based on the Shannon-Wiener heterogeneity index the value, Sumber Clangap had heterogeneity in-dex higher (H’=1,97) than Sumber Mangli (H’=1,39). Sumber Man-gli has a Java endemic species Paragomphus reinwardtii and Rhi-nocypha anisoptera that is spread only at Sumatera and East Java.

First record of the migrant dragonfly Pantala flavescens for mainland Italy (Insecta: Odonata)

In this contribution we report the observation of an individual of the migrant dragonfly Pantala flavescens (Fabricius, 1798) found at Montanaro (Piemonte, Italy) on 14 Aug 2019. This represents the first record of the species for mainland Italy and one of the very few available for Western and Central Europe before 2019. We discuss two hypotheses on the origin of this individual integrating available literature with very recent records retrieved from citizen science faunistic platforms.

JENIS-JENIS CAPUNG DI CAGAR ALAM TELUK KLOWE KECAMATAN ENGGANO KABUPATEN BENGKULU UTARA, PROVINSI BENGKULU

The purpose of this study is to find out the types of dragonflies (Odonata) found in the Klowe Bay Nature Reserve, Enggano sub-district, North Bengkulu Regency, Bengkulu Province. The study was conducted in the Klowe Bay Nature Reserve, Enggano sub-district, North Bengkulu Regency, Bengkulu Province in January 2018, which was identified in the Animal Physiology laboratory, Basic Science Biology, University of Bengkulu. by means of purposive sampling by surveying locations in accordance with dragonfly living conditions such as the presence of water and the presence of plants that are habitat for small insects that are food for dragonflies so that 2 points are determined. Dragonflies collected by exploring by swinging the net towards the dragonfly. The sample obtained is put into a killing bottle contains cotton in which the cotton has been given chloroform. The results obtained are 4 types of dragonflies with 15 individuals included in the Anisoptera sub-order. The types obtained include the Libellulidae Family, namely Orthetrum sabina, Pantala flavescens, Crocothemis Servilia, and Orthetrum glaucum.