Latin America has the planet’s largest land reserves for agriculture and had the most rapid agricultural expansion during the twenty-first century. A large portion of the expansion replaced forests, as shown by many local and regional studies. However, expansion varied regionally and also replaced other land covers. Further, it is important to distinguish between changes in cropland and pastureland as they produce food at different levels of efficiency and intensity. We used thirteen years (2001–2013) of MODerate Resolution Imaging Spectroradiometer satellite imagery to characterize cropland and pastureland expansion at multiple scales across Latin America. From 2001 to 2013, 17% of new cropland and 57% of new pastureland replaced forests throughout Latin America. Cropland expansion from 2001 to 2013 was less (44.27 Mha) than pastureland (96.9 Mha), but 44% of the 2013 cropland total was new cropland, versus 27% of the 2013 pastureland total, revealing higher regional expansion rates of row crop agriculture. The majority of cropland expansion was into pastureland within core agricultural regions of Argentina, Brazil, Bolivia, Paraguay, and Uruguay. On the contrary, pastureland largely expanded at frontiers, such as central Brazil, western Paraguay, and northern Guatemala. As others have suggested, regional agriculture is strongly influenced by globalization. Indeed, we find an overall decrease in agricultural expansion after 2007, coinciding with the global economic slowdown. The results illustrate agricultural cropland and pastureland expansion across Latin America is largely segregated, and emphasize the importance of distinguishing between the two agricultural systems, as they vary in land use intensity and efficiency.