GLM Model Research Articles

Habitat Suitability Modeling of Rare Turkeybeard (Xerophyllum asphodeloides) Species in the Talladega National Forest, Alabama, USA

This study focused on the rare and threatened plant species eastern turkeybeard (Xerophyllum asphodeloides (L.) Nutt.) and its presence or absence in the Talladega National Forest in Alabama, USA. An ensemble suitable habitat map was developed using four different modeling methods (MaxEnt, Generalized Linear Model, Generalized Additive Model, and Random Forest). AUC evaluation scores for each model were 0.99, 0.96, 0.98, and 0.99, respectively. Biserial correlation scores for models ranged from 0.71 (GLM) to 0.94 (RF). The four different models agreed suitable habitat was found to cover 159.57 ha of the land. The ground slope variable was the most contributive variable in the MaxEnt and RF models and was also significant in the GLM and GAM models. The knowledge gained from this research can be used to establish and implement habitat suitability strategies across the Talladega National Forest and similar ecosystems in the southern United States.

Long-Term Monitoring of Diel and Seasonal Rhythm of Dentex dentex at an Artificial Reef

Behavioral rhythms are a key aspect of species fitness, since optimize ecological activities of animals in response to a constantly changing environment. Cabled observatories enable researchers to collect long-term biological and environmental data in real-time, providing relevant information on coastal fishes’ ecological niches and their temporal regulation (i.e., phenology). In this framework, the platform OBSEA (an EMSO Testing-Site in the NW coastal Mediterranean) was used to monitor the 24-h and seasonal occurrence of an ecologically iconic (i.e., top-predator) coastal fish species, the common dentex (Dentex dentex). By coupling image acquisition with oceanographic and meteorological data collection at a high-frequency (30 min), we compiled 8-years’ time-series of fish counts, showing daytime peaks by waveform analysis. Peaks of occurrence followed the photophase limits as an indication of photoperiodic regulation of behavior. At the same time, we evidenced a seasonal trend of counts variations under the form of significant major and minor increases in August and May, respectively. A progressive multiannual trend of counts increase was also evidenced in agreement with the NW Mediterranean expansion of the species. In GLM and GAM modeling, counts not only showed significant correlation with solar irradiance but also with water temperature and wind speed, providing hints on the species reaction to projected climate change scenarios. Grouping behavior was reported mostly at daytime. Results were discussed assuming a possible link between count patterns and behavioral activity, which may influence video observations at different temporal scales.

The impact of occlusion therapy and predictors on amblyopia dose–response relationship

ABSTRACT This study aimed to calculate the dose–response relationship and predictors of visual acuity (VA) improvement following occlusion therapy at the IWK Health Center Eye Clinic and to add to amblyopia therapy dose–response relationship literature. A retrospective chart review was performed, considering patients who reached an occlusion therapy outcome at the IWK Eye Clinic between 2012 and 2019. The treatment outcome was defined as equal VA or stable VA for three consecutive clinical visits despite reported compliance. Subjective patching hours from parental reports, not prescribed hours, were used for statistical analyses. One hundred and thirty-four patients (66 females and 68 males) ages 2–11 years were included. Results showed a dose–response relationship of 224 hours/0.1logMAR increase in VA and total dose of 1344 hours for full-time occlusion and 504 hours for part-time occlusion was required to reach outcome VA. The fastest VA improvement occurred with younger age at treatment initiation, during the first 4 weeks of treatment, and in patients with strabismic and/or severe amblyopia. Classification of amblyopia, age, VA chart, initial distance VA (amblyopic eye), and treatment dose predicted the hour dose–response relationship. Dose–response relationship was faster in younger participants, in participants with strabismic and severe amblyopia, and during the first month of occlusion. Additionally, by creating a GLM model of dose–response relationship, relationship calculations can be performed. Therefore, an estimated timeline can be developed to allow allocation of clinical resources and to prepare patients for the treatment duration required and possibly increase treatment compliance.

Management of calves in commercial dairy farms in Mecklenburg-Western Pomerania, Germany and its impact on calf mortality and prevalence of rotavirus and Cryptosporidium parvum infections in pre-weaned calves

In a cross-sectional study, impact of management in dairy farms on calf mortality rates and prevalence of rotavirus and Cryptosporidium parvum in feces of calves was investigated. Sixty-two commercial dairy herds in Mecklenburg-Western Pomerania, Germany, were stratified selected in 2019. We performed in-person interviews and fecal specimens in samples of all-female calves of age 7 up to 21 days. Management data were documented on farm level. A Multiscreen Ag-ELISA was performed to determine rotavirus and Cryptosporidium parvum. Associations between two calf mortality rates, detection of C. parvum and rotavirus, and predictors were examined with GLM models. In farms with routine vaccination against respiratory diseases, 31-days mortality rate was 4.2% +/-1.26 compared to 7.6% +/-0.97 (p = 0.040) on non-vaccinating farms. Six-months mortality was lower in farms that continued feeding milk to calves during periods of diarrhea compared to farms that did not (6.9% +/-0.8 vs. 12.4% +/-2.3). In case of a routine shifting of calves from the calving box into calf boxes less C. parvum was detected compared to an individual moving of calves (33.3% +/-2.6 vs. 19.6% +/-5.3; p = 0.024). Our model confirms a positive association between occurrence of aqueous feces and frequency of detection of C. parvum (45.4% +/-23.6 vs. 21.4% +/-18.7; p < 0.001). Frequency of detection of rotavirus was lower in farms that reported a defined amount of applicated colostrum per calf than in farms that presented a range of colostrum instead of a defined amount. This study indicates the potential for mitigation of risk factors for mortality in calves.

Considering urban uses at a fine spatial resolution to understand the distribution of invasive plant species in cities

Cities are heterogeneous landscapes, composed of different urban uses with diverse histories. A thorough description of these urban landscapes is required to understand ecological patterns, particularly concerning high-stakes species such as invasive alien plants (IAP) which are abundant in cities. We assessed the effects of urban uses and linear structural elements on the distributions of seven IAP in a medium-sized French city (Blois). We examined how the relative contributions of these variables vary between three spatial scales of analysis (100 m, 200 m and 400 m) and three urban classifications. We characterized the use and age of urban neighborhoods through three classifications with different levels of details and described road and rail networks of Blois. We analyzed their effects on the distribution of seven invasive plants recorded throughout the whole city using GLM models. Urban use was the most significant variable in explaining IAP distribution. This variable was especially important at the finest spatial scale which allowed a detailed urban classification. Individual residential neighborhoods of different ages explained the distribution of Buddleja davidii, Berberis aquifolium and Acer negundo, whereas industrial areas explained Ailanthus altissima distribution. The effects of linear structural elements were lower and differed between species. We show that combining fine-scale spatial analyses with detailed characterization of urban use diversity is critical to understanding ecological patterns at the city scale. Investigating planting choices and dispersal process of IAP could complete our results. Urban descriptions based on explicit urban forms could also contribute to understanding species distribution in cities.

Abstract 101: Predicting Hematoma Expansion Using An Artificial Neural Network: An Exploratory Analysis Of The Atach 2 Trial

Introduction: Hematoma expansion (HE) in patients with intracerebral hemorrhage (ICH) is a key predictor of poor prognosis and potentially amenable to treatment. Current clinical scoring models, radiological markers, and labs have limited ability to predict HE. This study aimed to create a more robust classification model to predict HE in patients with ICH using a deep learning algorithm, an artificial neural network (ANN). Methods: Data from the ATACH 2 trial (Antihypertensive Treatment of Acute Cerebral Hemorrhage) was utilized. Variables included in the models were chosen as per literature consensus on salient factors associated with HE and clinical expertise. HE was defined as increase in either &gt;33% or 6mL in hematoma volume in the first 24 hours. A multi-layer feedforward ANN was trained using the back-propagation method to minimize the loss function with 5-fold cross validation. 80% of patients were used for training and 20% for testing. The ANN was compared to a logistic regression model (GLM). Given the imbalance in patients with HE, AUPRC (area under the precision-recall curve), recall and precision were calculated for the respective models. Results: Of the 963 patients in the study (mean age 62±13.0, 38.5% female), 31% had hematoma expansion. The median [interquartile range (IQR)] initial hematoma volume was 10.5mm 3 [5.16 - 20.25], median admission SBP [IQR] was 200 [184-217], median platelet count [IQR] was 213 [178-256], median INR [IQR] was 1.0 [0.9-1.0], and median admission GCS [IQR] was 15 [13-15]. The GLM model had AUPRC of 0.38 with recall of 0.60 and precision of 0.38 in the testing cohort. The ANN training model demonstrated AUPRC of 0.92 with recall of 0.72 with precision of 0.39 during testing. Initial ICH volume, platelets, INR, and GCS had the highest feature importance. Conclusion: We developed an ANN to predict HE in ICH patients performing with improved sensitivity and similar positive predictive value when compared to GLM. We support that ANN can capture complex associations between variables not attainable in classic regression models. This model may help identify patients at risk for HE who warrant careful monitoring and aggressive treatment upfront including those suitable for clinical trials for treatments.

Wildlife is imperiled in peri-urban landscapes: threats to arboreal mammals

Urbanization and deforestation impose severe challenges to wildlife, particularly for forest-living vertebrates. Understanding how the peri-urban matrix impacts their survival is critical for designing strategies to promote their conservation. We investigated the threats faced by brown howler monkeys (Alouatta guariba clamitans) in peri-urban regions of Rio Grande do Sul (RS) and Santa Catarina (SC) states, southern Brazil, by compiling negative interaction events (hereafter NIE) reported over more than two decades. We assessed the major NIEs, their distribution among age-sex classes, and the predictors of NIE-related mortality. After 20+ years of monitoring, we compiled 540 NIEs (RS = 248 and SC = 292). Electrocution by power lines was the most frequent cause of death or injury (37%), followed by dog attack (34%), vehicle collision (17%), and human mistreatment (12%). The occurrence of lethal injuries ranged from 5% to 69% depending on the type of NIE and on which state it occurred in. The overall post-NIE mortality was 56%. Adults of both sexes were the most affected individuals in both study regions. The minimal adequate GLM model explained 83% of the variation in NIE-related mortality. State, NIE type, and age-sex class were the main predictors of mortality. Overall, mortality was lower in SC and higher among adult females than in the other classes. We found that the survival of brown howler monkeys in the forest-urban interface is constrained by both the urban infrastructure and the growing interactions with humans and domestic and stray dogs (Canis familiaris). We propose the placement of aerial bridges, road signs and speed bumps in areas of frequent animal crossing, the sterilization of stray dogs, and the sensitization of local inhabitants on the importance of respecting and protecting wildlife to reduce their NIEs with humans and domestic animals in the forest-urban interface.

Sensory Characteristics, Nutritional Composition, and Quality of Eggs from Different Chickens

The present study evaluated the sensory, composition, and quality of eggs from Cosmopolitan (C), Improved Horro (H), ♂ Improved Horro * Cosmopolitan ♀ (HC), ♂ Cosmopolitan * Improved Horro ♀ (CH), Indigenous(L), and Koekoek (KK) genotypes. A completely randomized design was used in the study. A total of 108 (18/genotype), 180 (30/genotype), and 90 (15/genotype) eggs were used for the sensory, composition, and egg quality tests, respectively. All data were analyzed following the GLM model using SAS software. The boiled and scrambled eggs from L, H, and C were (P ≤ 0.001) preferred followed by CH, HC, and KK. Results showed that KK, HC, CH, and C eggs were higher in moisture, crude fat, crude ash, and crude fiber, but these traits were found lower in H and L genotypes. A significantly highest crude protein was observed in L (21.19 ± 0.19) genotype, with higher in H (20.62 ± 0.26), intermediate in HC (19.96 ± 0.29), C (19.85 ± 0.10), and CH (19.40 ± 0.37), whereas the eggs from KK genotype had the lowest crude protein content (18.69 ± 0.20). The egg quality was (P = 0.001 - 0.01) affected across traits except for eggshell indices (P > 0.05). The genotypes with a negative significant correlation with crude protein had a positive significant correlation with almost all composition and egg external quality traits. The genotypes with positive significant correlations of egg weight had positive significant correlations with most internal egg quality traits except that of yolk weight ratio, yolk albumen ratio, and yolk color. Conclusively: the eggs of L, H, and C genotypes were best favored followed by CH and C, but the KK genotype was the least favored, and these differences were deemed due to genetic variations, and interventions. Furthermore, the eggs laid from genotypes with deep yellow yolk color might be the most nutritious. It could also necessitate future breeding and dietary studies.

新疆典型蝗虫适生区分布预测

PDF HTML阅读 XML下载 导出引用 引用提醒 新疆典型蝗虫适生区分布预测 DOI: 10.5846/stxb202010192661 作者: 作者单位: 作者简介: 通讯作者: 中图分类号: 基金项目: 天山雪松项目（2020XS04）；新疆草原生物灾害遥感监测项目（2021） Prediction of potential living area of typical locusts in Xinjiang based on species distribution model Author: Affiliation: Fund Project: Tianshan Cedar Project 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:新疆草原面积广阔，农牧业地位突出，蝗灾对当地经济、生态威胁很大，近年新疆极端天气日渐频发，蝗灾监测与防治任务艰巨。以意大利蝗、西伯利亚蝗和亚洲飞蝗为代表的蝗虫数据为基础，综合考虑对蝗虫各生命周期有重要影响的环境因素，运用BIOCLIM模型、领域模型（DOMAIN）、马氏距离模型（MAHAL）、广义线性模型（GLM）、随机森林模型（RF）、提升回归树模型（BRT）、支持向量机模型（SVM）、最大熵模型（MaxEnt）等八种经典物种分布模型及集成模型对新疆典型蝗虫适生区展开了预测。结果表明：（1）不同模型对新疆典型蝗虫适生区预测存在差异，其中DOMAIN最差（曲线下面积（AUC）=0.688，真实技巧统计（TSS）=0.301），而提升回归树（BRT）最佳（AUC=0.920，TSS=0.910），基于BRT、SVM和MaxEnt 3个集成模型预测的新疆蝗虫适生区可靠性更高；（2）新疆典型蝗虫不同等级适生区面积约56.844万km2，占新疆总面积的36%，其中高适生区面积16.568万km2；（3）新疆典型蝗虫适生区主要集中于北疆阿勒泰、塔城地区，此外东部哈密地区及南疆绿洲边缘地带亦有分布。研究可为新疆草原工作部门推进蝗虫监测防治工作提供支持。 Abstract:Xinjiang grassland covers a vast area and occupies a prominent position in agriculture and animal husbandry. Locust plague is a great threat to the local economy and ecology. Furthermore, extreme weather in Xinjiang has become more frequent, the task of monitoring and preventing locust plagues is difficult. Based on the data of locusts represented by Italian locust, Siberian locust and Asian migratory locust, we comprehensively considered the environmental factors that had important influence on the different life cycles of locusts, and used eight typical species distribution models of BIOCLIM, DOMAIN, MAHAL, GLM, RF, BRT, SVM, MAXENT, and ensembled models to predict typical locust suitable areas in Xinjiang. The results showed that:(1) different models had differences in the prediction of typical locust suitable areas in Xinjiang, in which DOMAIN was the worst (AUC=0.688, TSS=0.301), and the BRT was the best (AUC=0.920, TSS=0.910). The prediction of the suitable area based on the three ensembled models of the BRT, SVM and MaxEnt was more reliable. (2) The suitable area of typical locusts of different grades in Xinjiang was about 568440 km2, accounting for 36% of the total area of Xinjiang, among which the high adaptable area was 165680 km2. (3) The typical locust breeding areas were mainly concentrated in the Altay and Tacheng areas of northern Xinjian. In addition, they were also distributed in Hami and the edge of oasis in southern Xinjiang. This study may provide support to the Xinjiang grassland authorities in their efforts to monitor and control locusts. 参考文献 相似文献 引证文献

Patterns of diversity, species richness and community structure in West African savannah small mammals (rodents and shrews)

Tropical savannah ecosystems are characterized by extensive grasslands with more or less sparse trees and thickets, and are threatened globally by anthropogenic forces. These grassland habitats house a rich and diversified fauna assemblage, with some of its conspicuous elements (for instance, small mammals) that have not been sufficiently investigated so far. In this paper, we meta-analyze the literature data available on the community structure and diversity patterns of shrews and rodents in West African savannahs. Overall, 10,197 small mammal individuals belonging to 111 species of Rodentia and 55 species of Soricomorpha were found in the various studies carried out in the countries covered by the present study. Studies using a combination of methods (e.g., live trapping, pitfalls, cover boards, visual encounter) detected more species in both Soricomorpha and Rodentia, and there was a positive survey (=trap ⁄ night) effort effect on the species richness in rodents. GLM models showed (i) that there was also no effect of trapping design (transect versus grid) on species richness per site, (ii) in both rodents and soricomorphs, the number of savannah species by country depended on the total species richness of that given country, but there was no effect of the relative surface covered by savannahs in that country. The number of sympatric species per site was 2.73± 1.7 (range = 1-7) in Soricomorpha and 6.33 ± 3.8 (range 1-15) in Rodentia. Dominance index was significantly different among countries, with Nigeria having lower values than all other countries and Ghana, Benin and Sierra Leone had significantly highest values. The conservation implications of the observed patterns are discussed.

​Genetic Polymorphism within Exon 3 of HSP90AA1 Gene and its Association Studies in Sahiwal and Crossbred Cows

Background: Heat shock proteins (HSPs) are molecular chaperones that play a critical role in recovering cells from stress and form a primary system for intra cellular self defense. They are highly conserved and play a crucial role in cellular thermo tolerance and heat stress response. Though there are many HSP genes, thermo tolerance is mainly correlated with HSP70 and HSP90 genes in Livestock species. Polymorphisms in these genes have shown an association with heat tolerance, milk production, fertility and disease susceptibility in livestock. They can be used as genetic markers for the selection of animals with better climate resilience, immune response and superior performance. Methods: The present study was carried out in Sahiwal (n=50) and Crossbred cows (n=50) with the objective to identify polymorphisms in HSP90AA1 gene. A 450 bp fragment of bovine HSP90AA1 gene covering exon3 was subjected to Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique to identify the polymorphism. PCR-SSCP patterns were correlated with the physiological, productive and reproductive traits in Sahiwal and crossbred cows using the univariate GLM model of SPSS 25. Result: The PCR-SSCP of exon 3 of HSP90AA1 gene yielded two conformational patterns AA and AB corresponding to two allelic variants A and B in both Sahiwal and crossbred cows. The allele frequencies of A and B were 0.78 and 0.22 and 0.84 and 0.16 in Sahiwal and crossbred cows, respectively. The association analysis of SSCP patterns revealed that genotype AA had higher lactation length in Sahiwal cows and higher total lactation milk yield and peak yield in crossbred cows.

Do greater levels of in-cage waking inactivity in laboratory mice reflect a spontaneous depression-like symptom? A pharmacological investigation

We previously identified in laboratory mice an inactive state [being awake with eyes open motionless within the home cage; inactive but awake, ‘IBA’] sharing etiological factors and symptoms with human clinical depression. We further test the hypothesis that greater time spent displaying IBA indicates a depression-like state in mice by investigating whether the antidepressant Venlafaxine, environmental enrichment, and their combination, alleviate IBA. Seventy-two C57BL/6J and 72 DBA/2J female mice were pseudo-randomly housed post-weaning in mixed strain-pairs in non-enriched (NE; 48 pairs) or in environmentally enriched (EE; 24 pairs) cages. After 34 days, half of the mice housed in NE cages were either relocated to EE cages or left in NE cages. For each of these conditions, half of the mice drank either a placebo or the antidepressant Venlafaxine (10 mg/kg). The 48 mice housed in EE cages were all relocated to NE cages and allocated to either the placebo (n = 24) or Venlafaxine (n = 24). IBA data were collected prior to and after environmental adjustment by trained observers blind to the pharmacological and environmental adjustment treatments. Data were analyzed using GLM models. NE cages triggered more IBA than EE cages (Likelihood-Ratio-Test Chi23 = 53.501, p < 0.0001). Venlafaxine and environmental enrichment appeared equally effective at reducing IBA (LRT Chi23 = 18.262, p < 0.001), and combining these approaches did not magnify their effects. Enrichment removal triggered IBA increase (LRT Chi21 = 23.050, p < 0.001), but Venlafaxine did not overcome the increase in IBA resulting from enrichment loss (LTR Chi21 = 0.081, p = 0.775). Theoretical implications for putative depression-like states in mice, and further research directions, are discussed.

Evolution of the IBEX-35 vs other international indices: determinants of market value according to XGBOOST and GLM models

PropósitoEl propósito de este artículo es estudiar la influencia de diferentes variables cuantitativas (tradicionalmente usadas) y cualitativas, como la posible influencia negativa en determinados períodos de ciertos factores sociopolíticos, sobre la formación del precio de.Diseño/metodología/enfoquePrimero analizamos de forma descriptiva la evolución del Ibex-35 en los últimos años y la comparamos con la de otros índices internacionales de referencia. A continuación, se han contrastado dos técnicas: un modelo estadístico clásico de regresión lineal (GLM) y un método basado en el aprendizaje automático denominado Extreme Gradient Boosting (XGBoost).ResultadosXGBoost nos permite obtener un modelo de predicción del valor de mercado muy preciso y claramente superior al otro, con un coeficiente de determinación cercano al 90%, calculado sobre las muestras de validación.Implicaciones prácticasDe acuerdo con nuestro análisis, la información contable individual es igual o más importante que la consolidada para predecir el comportamiento del precio de las acciones. Esto justificaría que España mantenga la obligación de presentar estados financieros intermedios individuales, lo que no ocurre en otros países de la Unión Europea porque la NIC 34 solo obliga a realizar estados financieros intermedios consolidados.Implicaciones socialesEl análisis descriptivo permite ver cómo el Ibex-35 se ha alejado de las tendencias internacionales, especialmente en periodos en los que se produjo algún hecho sociopolítico relevante, como el referéndum de autodeterminación de Cataluña, el doble proceso electoral de 2019 o la gestión inicial de la pandemia generada por el Covid-19.Originalidad/valorEn comparación con otras variables, el modelo XGBoost asigna poca importancia a los factores sociopolíticos cuando se trata de la formación del precio de las acciones; sin embargo, este modelo explica el 89.33% de su varianza.

Stress response markers in the blood of São Tomé green sea turtles (Chelonia mydas) and their relation with accumulated metal levels

Metals are persistent worldwide being harmful for diverse organisms and having complex and combined effects with other contaminants in the environment. Sea turtles accumulate these contaminants being considered good bioindicator species for marine pollution. However, very little is known on how this is affecting these charismatic animals. São Tomé and Príncipe archipelago harbours important green sea turtle (Chelonia mydas) nesting and feeding grounds. The main goal of this study was to determine metal and metalloid accumulation in the blood of females C. mydas nesting in São Tomé Island, and evaluate the possible impacts of this contamination by addressing molecular stress responses. Gene expression analysis was performed in blood targeting genes involved in detoxification/sequestration and metal transport (mt, mtf and fer), and in antioxidant and oxidative stress responses (cat, sod, gr, tdx, txrd, selp and gclc). Micronuclei analysis in blood was also addressed as a biomarker of genotoxicity. Present results showed significant correlations between different gene expressions with the metals evaluated. The best GLM models and significant relationships were found for mt expression, for which 78% of the variability was attributed to metal levels (Al, Cu, Fe, Hg, Pb and Zn), followed by micronuclei count (65% - Cr, Cu, Fe, Hg, Mn and Zn), tdx expression (52% - Cd, Fe, Mn, Pb and Se), and cat expression (52% - As, Fe, Se and Cd x Hg). Overall, this study demonstrates that these green sea turtles are trying to adapt to the oxidative stress and damage produced by metals through the increased expression of antioxidants and other protectors, which raises concerns about the impacts on these endangered organisms' fitness. Furthermore, promising biomarker candidates associated to metal stress were identified in this species that may be used in future biomonitoring studies using C. mydas’ blood, allowing for a temporal follow-up of the organisms.

Are Introduced Alien Species More Predisposed to Invasion in Recipient Environments If They Provide a Wider Range of Services to Humans?

The drivers of invasion success of alien species remain, to some extent, a matter of debate. Here, we suggest that the services (the benefits humans obtain from a species) provided by alien plants could predict their invasion status, such that alien species providing more services would be more likely to be invasive than not. The rationale for this expectation is that alien species providing multiple services stand a better chance of being introduced in various numbers and multiple times outside their native range (propagule pressure theory). We investigated this hypothesis on alien woody species in South Africa. First, we defined 12 services provided by all the 210 known naturalized alien woody plants in South Africa. Then, we tested for a phylogenetic signal in these services using a DNA barcode-based phylogeny. Finally, we tested for potential links between the services and invasion status by fitting GLM models with appropriate error families. We found a phylogenetic signal in most services, suggesting that closely related species tend to provide similar services. Counter-intuitively, we consistently found that alien non-invasive species tend to provide more services, or even unique services, in comparison to alien invasive species. Although alternative scenarios are plausible to explain this unexpected finding, we speculate that harvesting alien plants for human benefits may limit their invasion ability. This warrants further investigation.

Quantifying bycatch risk factors for the Chinese distant water fishery

AbstractMitigating bycatch of non-target fish species is a common objective in fisheries management that may be supported by the gathering of data from fishery observer programs and quantitative analysis of bycatch risk factors. We build three GLM models based on Chinese tuna longline fishery observer data in terms of analysis of total bycatch rate (TB rate), total bycatch ratio (TB ratio), and species-specific bycatch rate, respectively. The positive log-linear models assumed a Gaussian observation error model and a linear combination of categorical independent variables, including area, year, month, depth, and bycatch species. Results show that distributions of TB rate and TB ratio followed different trends and a latitudinal decrease was observed from both the northern and southern hemisphere of the equator. Comprehensively, the Pacific is a better place to fish compared to the Indian and Atlantic Oceans in terms of relatively lower TB rate and TB ratio. Fishing in open oceans can somehow avoid a high TB ratio than fishing in coastal waters. As a result, we recommend area 2SW, 2SE, 2 NW, 14SW, 14SE, and 14 NW as appropriate fishing ground for Albacore (Thunnus alalunga) while area 11N, 11S, 18SW, and 18SE to be appropriate fishing ground for fishing bigeye tuna (Thunnus obesus). Setting fishing gears deeper than 500 m would also help to get a low TB rate and TB ratio.

Association of SSCP Polymorphisms of HSP70 Gene with Physiological, Production and Reproduction Performance in Sahiwal and Crossbred Cows

Background: Cellular tolerance to heat stress is mediated by heat shock proteins (HSPs). The HSPs act as molecular chaperones and are transcribed in response to stress. Among different families of these proteins, HSP70 is considered to be related to the development of temperature tolerance. Unraveling polymorphism in heat shock protein genes could be a step towards the identification of genetic markers for selecting heat-tolerant cattle. Methods: The present study was carried out in Sahiwal (n=50) and Crossbred cows (n=50) with the objective to identify polymorphisms in HSP70 gene. Two fragments (295 and 220 bp) of HSP70 gene were subjected to Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique. Statistical analysis was performed to study the association of each SSCP genotype on physiological, production and reproduction traits in Sahiwal and crossbred cows using the univariate GLM model of SPSS 25. Result: The PCR-SSCP of 295 bp fragment of HSP70 gene revealed two genotypes AA and AB in Sahiwal cows and two genotypes AA and AC in crossbred cows. The association analysis revealed that genotype AA had higher peak milk yield in Sahiwal cows while the same genotype had higher total lactation milk yield, lower service period and calving interval in crossbred cows. The 220 bp fragment was found to be monomorphic in both Sahiwal and crossbred cows.

PSXII-18 Immunoglobulin G content and composition of colostrum in different cattle breeds

Abstract Immunoglobulins (Ig) are essential bioactive components in colostrum that enable passive immunization of newborn calves. Compared to mature milk, concentrations of fat and protein are greater in colostrum, and represent a vital source of energy and nutrients. Colostral IgG was shown to vary between individual dairy cows, but comparative data on different breeds and performance levels are scarce. The objective of the present field study was to investigate the contents of total IgG, fat, protein, and lactose in colostrum in different Swiss and German dairy and dual-purpose breeds. We collected colostrum samples of 458 cows of 13 different breeds (dairy breeds: Brown Swiss, Swiss and German Holstein Friesian, and New Zealand Holstein; dual-purpose breeds: German Fleckvieh, Holstein Friesian × Montbéliarde, Montbéliarde, Murnau-Werdenfels, Original Braunvieh, Pinzgauer, Rhetic Gray, and Simmental; beef type crossbred Charolais × Holstein Friesian). Colostrum samples were obtained between 5 and 900 min after calving and analyzed for total IgG, fat protein, and lactose contents. Statistical analysis was carried out using a GLM model with breed, parity number, and time of colostrum collection as fixed effects. Immunoglobulin G concentrations varied between 12.7 and 204.0 mg/mL. No effect of breeding purpose (i.e., dairy or dual-purpose), nor of previous lactation yield on IgG content was observed. However, milking of cows for the first time later than 12 h after parturition resulted in lower colostrum IgG concentrations compared to colostrum harvest within 9 h after calving (P &amp;lt; 0.05). Multiparous cows had a higher colostral IgG concentration than primiparous cows (P &amp;lt; 0.0001). Overall, concentrations of IgG and other constituents in colostrum varied widely in the different cattle breeds. High-yielding dairy cows did not have poorer colostrum quality compared to lower yielding animals or beef and dual-purpose breeds, which suggests an individually different transfer of circulating IgG into colostrum.

PSIII-13 The Effect of the inclusion of liquid brewers yeast during gestation on colostrum quality and passive transfer of immunity in lambs

Abstract Fourteen Suffolk/Suffolk x Hampshire pregnant ewes were utilized to evaluate the effects of liquid brewers yeast on colostrum quality and the transfer of passive immunity in their offspring. Parainfluenza type 3 (PI3) vaccination was used elicit an immune response. Ewes were vaccinated with PI3 at 33 and 18 d prior to expected lambing day. Ewes were randomly assigned to one of two treatments: late gestation diet (CON) or late gestation diet supplemented with liquid brewers yeast at an average rate of 60 g DM/d (LBY). Ewes were fed treatment diets from 45 d prior to expected lambing to 3 d post lambing. Blood was collected from ewes’ via jugular venipuncture prior to vaccination and within 6 hours postpartum. Blood was collected also from the jugular vein of lambs (n = 22) at 3 d of age. Ewe and lamb blood serum was tested for Bovine Parainfluenza 3 Virus and data on anti-PI3 antibody titers was evaluated. Colostrum was collected within 4 hours postpartum and analyzed for colostral IgG concentrations using a refractometer in % Brix. A PROC GLM model was performed in SAS 9.4, where the model for ewe colostrum quality and lamb anti-PI3 titers analyses included the main effect of treatment diet. All vaccinated ewes were positive for PI3 antibodies (&amp;gt;64 titer) immediately prior to and post lambing. All lambs tested positive for antibody titers for PI3 with no differences between treatment (197.3 for CON and 160.8 for LBY; P = 0.43). The colostral IgG concentrations did not differ between CON (30.4% Brix) and LBY (24.6% Brix) ewes(P = 0.21). These results indicate that, although there were numerical differences, the addition of LBY to the ewes’ diet during late gestation did not significantly improve colostrum quality or passive transfer of immunity in lambs.

Age- and sex-specific strategies of spring migration in the masked shrike are reflected in a differential response to winter climate conditions

Adult males of many migratory species arrive at breeding grounds before females and young. In a 34-year study of the masked shrike, Lanius nubicus, an age- and sex-specific pattern of spring arrival was distinguished. Adult males arrived first followed by juvenile males and adult females, whereas juvenile females appeared last. We hypothesized that these differences in migratory strategies would be reflected in a differential response to climate conditions at the wintering grounds. Testing correlations between spring arrival time and winter climate conditions provided strong support to our hypothesis. Adult males’ arrival time exhibited high associations with climate conditions in early spring, upon migratory take-off, whereas juvenile males responded mostly to conditions in November, upon autumn arrival in Africa. Adult females responded to both parameters in autumn and early spring, whereas young females’ arrival correlated only with a few variables in autumn. GLM models of median spring arrival day for all categories but the young females were highly statistically significant with adjusted R-squared values of 0.81–0.93. The emerging pattern of different associations between timing of spring migration and climate conditions at the wintering grounds sheds new light on existing evolutionary theories regarding age- and sex-specific migratory strategies.