Microstructure measurements of temperature and current shear are made using an autonomous underwater glider. The glider is equipped with fast-response thermistors and airfoil shear probes, providing measurements of dissipation rate of temperature variance, χ, and of turbulent kinetic energy, ε, respectively. Furthermore, by fitting the temperature gradient variance spectra to a theoretical model, an independent measurement of ε is obtained. Both Batchelor (εB) and Kraichnan (εK) theoretical forms are used. Shear probe measurements are reported elsewhere; here, the thermistor-derived εB and εK are compared to the shear probe results, demonstrating the possibility of dissipation measurements using gliders equipped with thermistors only. A total of 152 dive and climb profiles are used, collected during a one-week mission in the Faroe Bank Channel, sampling the turbulent dense overflow plume and the ambient water above. Measurement of ε with thermistors using a glider requires careful consideration of data quality. Data are screened for glider flight properties, measurement noise, and the quality of fits to the theoretical models. Resulting dissipation rates from the two independent methods compare well for dissipation rates below 2×10−7 W kg−1. For more energetic turbulence, thermistors underestimate dissipation rates significantly, caused primarily by increased uncertainty in the time response correction. Batchelor and Kraichnan spectral models give very similar results. Concurrent measurements of ε and χ are used to compute the dissipation flux coefficient Γ (or so-called apparent mixing efficiency). A wide range of values is found, with a mode value of Γ≈0.14, in agreement with previous studies. Gliders prove to be suitable platforms for ocean microstructure measurements, complementary to existing methods.