INTRODUCTION: Presence of ferrule during delivery of a fixed prosthesis onto an endodontically treated tooth is important for fracture resistance of the tooth in function. AIM: This study aims to investigate the location of ferrule on fracture resistance of endodontically treated extracted mandibular premolars. MATERIALS AND METHOD: Sixty extracted mandibular premolars were divided into 6 groups where each group consisted of 10 teeth. The groups were as follows: control group, GHT; endodontically treated teeth without endodontic posts and crowns, GCF; teeth with a 2 mm circumferential ferrule, GBF; teeth with a 2 mm buccal ferrule; GLF, teeth with a 2 mm lingual ferrule; GBLF, teeth with a 2 mm buccal and lingual ferrule; and teeth without ferrule, GWF. All the teeth were endodontically treated and glass fibre posts were cemented and crowns were luted. All the teeth were loaded in a universal testing machine until fractured. Fracture lines were also assessed according to their location onto the teeth. The results were recorded and were statistically analyzed. RESULTS: Mean ±SD loads for the groups ranged from 770.3 ±212.9 N to 1008.1 ±176.5 N. One way ANOVA revealed a statistically significant difference between the groups (P_.05). However, no statistically significant differences were observed among groups (P>.05), except between GHT (control group) and group GWF (without ferrule). CONCLUSION: The study reported that although the presence of ferrule leads to improved fracture resistance, specific location of the ferrule had no significant differences in the fracture strength of endodontically treated teeth restored with glass fiber posts.