Glass Carbon Electrode Research Articles

Electrochemical Preparation of Gold Nanoparticles-Polypyrrole Co-Decorated 2D MoS2 Nanocomposite Sensor for Sensitive Detection of Glucose

A sensitive electrochemical sensor was fabricated based on two dimensional (2D) MoS2 nanosheets (MoS2), gold nanoparticles (AuNPs) and polypyrrole (PPY) nanocomposite modified glass carbon electrode (GCE) for glucose detection. Liquid phase exfoliation (LPE) method was applied for the synthesis of 2D MoS2 nanosheets using N, N-dimethylformamide (DMF). The fabrication of sensor involved the electrochemical deposition of AuNPs on GCE using cyclic voltammetry (CV) followed by drop-casting of 2D MoS2 nanosheets to prepare MoS2-AuNPs/GCE electrode. Pyrrole was then electropolymerized on MoS2-AuNPs/GCE surface using amperometry. The structural properties of prepared nancomposites were characterized by field emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD) and fourier transform infra red (FTIR) spectroscopic techniques. The prepared MoS2-PPY-AuNPs/GCE electrode was applied for electrochemical sensing of glucose by differential pulse voltammetry (DPV) in NaOH solution. Cu(II)/Cu(III) redox couple was utilized as catalytic center which converted glucose into gluconolactone at an oxidation potential of +0.45 V. The fabricated electrode showed a detection limit (LOD) of 0.08 nM and a quantification limit (LOQ) of 0.26 nM. Furthermore, it exhibited high reproducibility (2.48%), long term stability and high selectivity toward glucose detection. The electrode was found suitable for glucose detection in human serum samples with recoveries ranging from 97%–102.1%.

Tyrosinase Incorporated with Au-Pt@SiO2 Nanospheres for Electrochemical Detection of Bisphenol A

Based on tyrosinase modified Au-Pt@SiO2/Au-graphene (APS/Au-GN) electrode, a highly sensitive biosensor was developed for the direct electrochemical detection of bisphenol A (BPA) in food matrices. In this work, Au-graphene (Au-GN), Au-Pt@SiO2 nanocomposites (APS NPs) and tyrosinase (Tyr) were subsequently cast on glass carbon electrode (GCE) via the electrostatic interaction and Van der Waals force. The self-assembled APS NPs and Tyr were decorated on Au-GN forming a new hierarchical three dimensional nanostructure. Due to the dense Au/Pt dots distributed on silica nanoparticles, APS NPs could behave excellent catalytic activity and stability. In this regard, BPA could be sensitively detected by square wave voltammetry (SWV) and differential pulse voltammetry (DPV) with well-defined oxidation peaks at 480 mV and 660 mV, respectively. Under the optimal conditions, SWV detection behaved good analytical performance toward BPA with a wide linear response ranging from 0.01 mg L−1 to10 mg L−1. The detection limit of APS/Au-GN/GCE for BPA was 1.80 μg L−1 (S/N = 3). Moreover, the method showed good reproducibility in practical samples with recoveries from 87.4% to 110.7%, suggesting its potential application in food analysis and safety verification.

Fluoride-capped nanoceria as a highly efficient oxidase-mimicking nanozyme: inhibiting product adsorption and increasing oxygen vacancies.

Nanozymes aim to mimic enzyme activities using nanomaterials. Nanoceria (CeO2 nanoparticles) is an important model nanozyme for its rich redox chemistry. In particular, its oxidase-like activity allows oxidation reactions without the need of unstable and toxic H2O2. Fluoride can significantly improve its oxidase-like activity, and this work aims to understand the mechanism of fluoride-promoted catalysis. First, fluoride can adsorb on CeO2 tighter than other halides, but not as strong as phosphate as characterized by isothermal titration calorimetry (ITC). FT-IR spectroscopy indicates adsorption of fluoride likely via exchange with surface hydroxide groups. Fluoride capping inverses the surface charge of CeO2, facilitating desorption of the ABTS oxidation product, significantly increasing the turnover number. The Raman, EPR and XPS spectroscopy results demonstrate that the concentration of Ce3+ and the accompanying oxygen vacancy significantly increased upon adding F-, which can explain the enhanced catalytic activity. Finally, the electron transfer properties of fluoride-capped CeO2 were more efficient than that of the bare CeO2 as determined by a direct electrochemical measurement on a glass carbon electrode. This study has provided new insight into nanoceria, and can also further confirm the role of nanoceria as a model for engineering the surface of nanozymes.

An electrochemical sensor based on poly(procaterol hydrochloride)/carboxyl multi-walled carbon nanotube for the determination of bromhexine hydrochloride.

Poly(procaterol hydrochloride) (p-ProH) polymeric film was successfully deposited onto the carboxyl multi-walled carbon nanotube (CMWCNT) modified glass carbon electrode (GCE) to construct a p-ProH/CMWCNT composite modified GCE. Due to the synergistic effect of p-ProH and CMWCNT in the composite, the developed sensor can enormously enhance the oxidation peak current of bromhexine hydrochloride (BrH) at ca. + 0.90 V. Based on this appearance, an electrochemical method was established for the sensitive and selective determination of BrH with differential pulse voltammetry (DPV). Various conditions affecting the peak current response of BrH were studied and optimized. Under the best conditions, the oxidation peak current of BrH is linear to its concentration in two linear dynamic ranges of 0.2–1.0 μmol L−1 (R = 0.9948) and 1.0–8.0 μmol L−1 (R = 0.9956), with a detection limit of 0.1 μmol L−1 (S/N = 3). Interference experiment indicated that the as-prepared electrochemical sensor showed wonderful selectivity to the recognition of BrH and was free from disturbance of many other electro-active substances such as dopamine, ascorbic and uric acid. Finally, the practicability of the BrH sensor was verified by the satisfactory results acquired from the BrH determination in pharmaceutical preparation and human serum.

Fabrication of Electro-Active Pt/IMo6O24/Graphene Oxide Nanohybrid Modified Electrode for the Simultaneous Determination of Ascorbic Acid, Dopamine and Uric Acid

A novel electrochemical active electrode based on isopolyacid ion IMo6O245- (IMo6), Pt and graphene oxide (GO) had been fabricated, which was used as a biosensor in double signal amplification platform for simultaneous detection of ascorbic acid (AA), dopamine (DA) and uric acid (UA). Cyclic voltammetry, amperometry and differential pulse voltammetry indicated that a glass carbon electrode modified with Pt/IMo6/GO (Pt/IMo6/GO-GCE) presented higher electrochemical activity toward the oxidation of AA, DA and UA and faster response than those of a bare GCE, GO-GCE, Pt/GO-GCE and IMo6/GO-GCE. As an electrochemical sensor, the Pt/IMo6/GO-GCE showed a wide linear range of 50–4000 μM, 4–750 μM, and 75–300 μM with the limits of detection (S/N = 3) as 6.4 μM, 0.22 μM and 0.72 μM, corresponding to AA, DA and UA, respectively. In addition, the Pt/IMo6/GO-GCE provided a new option in the simultaneous determination of AA, DA and UA in human blood serum with reliable recovery.

Bimetallic MOF-Based Enzyme-Free Sensor for Highly Sensitive and Selective Detection of Dopamine

A bimetallic metal organic frameworks termed as Fe2Ni MIL-88B was fabricated as an enzyme-free dopamine (DA) sensor, which was designed with crystalline porous structure and synthesized by a facile hydrothermal technique. To gain insight into the structure and morphology of Fe2Ni MIL-88B, a series of characterizations such as fourier transform infrared (FTIR), X-ray diffraction (XRD), transmission electron microscopic (TEM) and field emission scanning electron microscopic (FE-SEM) had been performed, proving the as-prepared composite owned hexagonal rod-like structure with the size 633 nm–967 nm in length and 100 nm–167 nm in width. The Fe2Ni MIL-88B modified glass carbon electrode (Fe2Ni MIL-88B/GCE) possessed an excellent electrochemical catalytic performance to DA which are derived from electrochemical test, having been reported to own wider linear range from 1.2 μM–1.8 mM, lower detection limit of 0.40 μM (S/N = 3) and higher sensitivity of 124.7 μA mM−1 cm−2. The proposed sensor exhibited acceptable stability, repeatability and achievability for analyzing real samples. All the evidence suggested a potential application of Fe2Ni MIL-88B in DA detection.

Electrochemiluminescence response of a benzouril-constructed electrode to bipyridyl herbicides

An electrochemiluminescence sensor with modification of macrocyclic benzo[6]uril on the surface of a glass carbon electrode was achieved, which has been applied for the quantitative analysis of paraquat and diquat.

A Highly Sensitive and Stable Dopamine Sensor Using Shuttle-Like α-Fe2O3 Nanoparticles/Electro-Reduced Graphene Oxide Composites

The development of sensitive and stable electrochemical sensors toward dopamine is highly desirable. Herein, a novel voltammetric sensor was developed for the detection of dopamine using shuttle-like α-Fe2O3 nanoparticles/electro-reduced graphene oxide nanocomposites (S-Fe2O3/ErGO). The surface morphology, microstructure, and chemical composition of S-Fe2O3/ErGO nanocomposites were investigated by SEM, XRD and Raman spectra, respectively. The electrochemical behavior of Fe2O3/ErGO nanocomposite modified glass carbon electrode (S-Fe2O3/ErGO/GCE) was studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), and the results show that S-Fe2O3/ErGO nanocomposites have large electrochemical active area and low charge transfer resistance. The anodic peak current of S-Fe2O3/ErGO/GCE increases 1-fold and 37-fold compared to that of ErGO/GCE and S-Fe2O3/GCE, respectively, suggesting the remarkably synergistic enhancement effect on the electrooxidation of dopamine. The anodic peak current is proportional to the concentration of dopamine over the range of 0.01 μM–2 μM. However, the logarithm of anodic peak current versus logarithm of dopamine concentration is linearly related in the range of 2 μM–80 μM. The detection limit (3σ/s) and sensitivity are estimated to be 1.15 nM and 95.57 μA μM−1 cm−1, respectively. Moreover, S-Fe2O3/ErGO/GCE also showed good anti-interference, reproducibility and stability. Finally, the proposed S-Fe2O3/ErGO/GCE was successfully employed to determinate dopamine in the human serum samples with satisfactory results.

Gold-nanoparticles-graphene modified glassy carbon electrode for trace detection of lead ions

In order to fulfill rapid and trace detection of heavy metals, in the study, gold-nanoparticlesgraphene modified glass carbon electrodes were used to detect lead(Pb(II)) ions. The lead ion (Pb(II)) standard sample in acetic acid-sodium acetate buffer solution was detected by the modified electrode, and the detection conditions were optimized. Under the optimized conditions, in the buffer solution with pH=4.5, the lead ions peak current showed excellent linear relationship with the lead ion concentration in the range of 0.2~50μg/L (R2=0.995), and the minimum detection limit was 0.34μg/L. At the same time, the actual water samples were determined by nano-gold-graphene/glass carbon electrodes for lead ions, and the recovery ranges was 92.94%~101.47%, which showed that the gold-nanoparticles-graphene/glass carbon electrodes possessed significant detection performance for the actual water samples.

Electrochemically Recognizing Tryptophan Enantiomers based on Carbon Black/Poly-l-Cysteine Modified Electrode

Construction of efficient and convenient sensors for recognition of chiral enantiomers is of much significance in the field of electrochemistry and life sciences. Herein, we designed an effective chiral interface for the successful identification of tryptophan (Trp) enantiomers by electrodepositing l-cysteine on the surface of glass carbon electrode modified with carbon black. The structure and morphology of the prepared sensor was characterized by scanning electron microscopy (SEM) and electrochemical methods. Compared with l-tryptophan (L-Trp), the sensor exhibits favorable chiral recognition towards D-tryptophan (D-Trp) with a separation coefficient of 2.50. Compared with existing studies on electrochemical chiral recognition of Trp enantiomers, our designed chiral platform boasts of a wider linear range (1.0–1400.0 μM) and lower limits of detection thus, 0.33 μM and 0.45 μM for L-Trp and D-Trp respectively. In addition, the proposed sensor has the capacity to recognize chiral molecules in Trp isomers mixture and the potential for the identification of other amino acids.

In-situ Polymerization of N-(3-aminobenzyl)-N, N-dimethyl-N-dodecyl Ammonium Bromide on Glassy Carbon Electrode for Estradiol Detection

Estradiol is a common endocrine disrupting chemicals. Therefore, it is necessary to develop simple, sensitive and rapid methods for testosterone bioanalysis. In this work, poly (N-(3-aminobenzyl)-N,N-dimethyl-N-dodecyl ammonium bromide) (PAS), a novel cathodic organic electrochemiluminescence (ECL) emitter, was synthesized by in-situ polymerization of the corresponding monomer on glass carbon electrode. The PAS ECL system was constructed with the PAS modified electrode, and potassium persulfate (KPS) as oxidizing agent and co-reactant. It was applied to the ECL detection of estradiol. According to the results of fluorescence and dynamic light scattering (DLS), mixed micelle of PAS and estradiol was formed. The combination of PAS with estradiol intensified the fluorescence of the ECL system and enlarged the colloidal size. Hydrogen bonds and electrostatic interaction may play a role in the combination. The linearity for estradiol quantification is 6 pM to 1.5 nM with a LOD of 0. 1 pg/mL. The modified electrode can be easily renewed by washing with ethanol and water and the relative error in estradiol measurement does not exceed ± 7% in 7 time recycling. The detection of estradiol in urine sample qualified the ECL system with a recovery of 94.5 to 105% and the calculated coefficient of variation of 3.8%. This indicates that the PAS ECL system has the potential to detect estradiol in urine.

Construction of Electrochemical Immunosensor Based on Gold-Nanoparticles/Carbon Nanotubes/Chitosan for Sensitive Determination of T-2 Toxin in Feed and Swine Meat.

T-2 toxin (T-2) is one of major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products. Thus, a facile and sensitive method is essential for accurate T-2 toxin detection. In our work, a specific electrochemical immunosensor based on gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite was established. The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode. Afterwards, the alkaline phosphatase labeled anti-mouse secondary antibody was bound to the electrode surface by reacting with the primary antibody. Lastly, alkaline phosphatase catalyzed the hydrolysis of the substrate α-naphthyl phosphate, which produced an electrochemical signal. Compared with conventional methods, the established immunosensor was more sensitive and simpler. Under optimal conditions, this method could quantitatively detect T-2 from 0.01 to 100 μg·L−1 with a detection limit of 0.13 μg·L−1 and favorable recovery 91.42–102.49%. Moreover, the immunosensor was successfully applied to assay T-2 in feed and swine meat, which showed good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Electrochemical Sensing of Heavy Metal Ions based on Monodisperse Single-crystal Fe3O4 Microspheres

Single-crystal Fe3O4 with monodisperse microspheres structure has been used for individual electrochemical detection of heavy metal ions. Morphology and structure of the as-prepared Fe3O4 microspheres were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD). Meanwhile the electrochemical properties of the Fe3O4 microspheres modified glass carbon electrodes (GCE) were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), and the enhanced electrochemical response in stripping voltammetry for individual detection of Pb(II), Hg(II), Cu(II), and Cd(II) was evaluated using square wave anodic stripping voltammetry (SWASV). With high specific surface area and excellent catalytic activity toward heavy metal ions, the as-prepared monodisperse and single-crystal Fe3O4 microspheres show a preferable sensing sensitivity (22.2 μA/μM) and limit of detection (0.0699 μM) toward Pb(II). Furthermore, the electrochemical sensor of Fe3O4 microspheres exhibits excellent stability and it also offers potential practical applicability for the determination of heavy metal ions in real water samples. This study provides a potential simple and low cost iron oxide for the construction of sensitive electrochemical sensors applied to monitor and control the pollution of toxic metal ions.

Aptamer-Based Electrochemical Biosensor for Mercury Ions Detection Using AuNPs-Modified Glass Carbon Electrode

A mercury ion aptamer electrochemical biosensor based on a Thymine-Hg2+-Thymine (T-Hg2+-T) structure has been constructed and successfully used to detect mercury ions in tap water samples. The aptamer electrochemical biosensor was assembled using a mercury ion aptamer-functionalized AuNPs-modified glass carbon electrode (aptamer/(AuNPs/CS)₂/GCE) capable of specifically detecting mercury ions through a T-Hg2+-T structure. The experimental results indicated the optimum electrochemical performance of the prepared aptamer biosensor when the (AuNPs/CS)₂/GCE surface was modified with 1.0 μM aptamer and incubated with Hg2+ for 60 min. Moreover, the aptamer biosensor exhibits a good linear relationship between the logarithm of the Hg2+ concentration and the DPV peak current in the range from 0.01 to 500 nM following the linearization equation Ip (μA) = 2.59902 + 0.2097logC (R² = 0.9994) with a limit of detection as low as 0.005 nM. Therefore, the constructed aptamer biosensor provides a simple and sensitive approach for Hg2+ detection in aqueous solution with promising application for trace Hg2+ detection in real samples.

A Label-Free Electrochemical Biosensor for Kanamycin Determination Based on Single-Stranded DNA Immobilized on Graphene-Cds-Chitosan Modified Electrode

Background: Overuse of antibiotics has become a serious ecological problem worldwide. It is necessary to monitor and control the pollution of antibiotics. Kanamycin is a representative antibiotic and has widely been used to treat a wide variety of infections. Although different methods have been established for kanamycin detection, it is still of great significance to develop new sensing approach to kanamycin monitoring. Methods: Graphene and Cadmium Sulphide (GR-CdS) nanocomposites were dispersed in 0.035% chitosan (CHIT) aqueous solution containing 0.52% acetic acid. Then, Glass Carbon Electrode (GCE) surface was coated with 5 µL of 1 mg·mL-1 GR-CdS suspension. The GR-CdS-CHIT modified electrode was immersed in 0.5 mg mL-1 single-stranded DNA (ssDNA) solution and 0.5 V potential was applied for 200 s to immobilize ssDNA on the electrode surface. Results: GR-CdS-CHIT dramatically promoted the electrochemical oxidation response of guanine residue in DNA. ssDNA exhibited higher oxidation peak current than double-stranded DNA (dsDNA). Under optimal conditions, ssDNA/GR-CdS-CHIT/GCE was prepared and used as the sensor to determine kanamycin, which showed a linear response to kanamycin in the concentration range of 0.10 to 1.0 mg mL-1. The proposed sensor was successfully applied to monitoring photodegradation of kanamycin. Conclusion: ssDNA was effectively immobilized on GR-CdS-CHIT modified electrode and exhibited high electrochemical oxidation response. When kanamycin was present in the solution, the electrochemical oxidation peak current of ssDNA on the modified electrode declined due to the interaction between kanamycin and DNA. Thus, a novel electrochemical DNA biosensor for kanamycin was successfully developed. Keywords: CdS, differential pulse voltammetry, graphene, kanamycin, photodegradation, single-stranded DNA.

Voltammetric Study of Sudan I on Glass Carbon Electrode and Its Interaction with DNA

Voltammetric Study of Sudan I on Glass Carbon Electrode and Its Interaction with DNA

Scalable Dealloying Route to Mesoporous Ternary CoNiFe Layered Double Hydroxides for Efficient Oxygen Evolution

The mass production of clean hydrogen fuels via (photo)electrochemical water splitting calls for highly efficient, cost-effective, and eco-friendly catalysts. Herein, a facile and scalable strategy, namely, dealloying, is advanced to fabricate mesoporous ternary layered double hydroxides (LDHs) containing Co, Ni, and Fe for highly efficient oxygen evolution and overall water splitting, based upon elaborate design of precursors and accurate control of the dealloying process. The Co1Ni2Fe1-LDH exhibits remarkable catalytic properties toward oxygen evolution reaction in 1 M KOH, for instance low overpotentials (only requires 240.4 mV on glass carbon electrode, and 228.5 mV on Ni foam to drive 10 mA cm–2), a small Tafel slope (38.6 mV dec–1), as well as excellent stability (lasts 45 h for 10 mA cm–2 without deactivation). Surprisingly, a symmetric alkaline electrolyzer constructed with Co1Ni2Fe1-LDH serving as the catalyst for both cathode and anode requires only 1.65 V to drive 10 mA cm–2. The distinguished ...

Promising copper oxide-histidine functionalized graphene quantum dots hybrid for electrochemical detection of hydroquinone

Hybrid of graphene with metal oxide has become one research hotspot in material science, because the hybrid may pave one new way to improve their electronic, chemical and electrochemical properties. The study reports one strategy for synthesis of copper oxide-histidine functionalized graphene quantum dots (CuO-His-GQD). First, Cu2+ ions react with His-GQDs to form Cu-His-GQD complex via the coordination between Cu2+ and His-GQD. The complex is oxidized in air to produce CuO-His-GQD hybrid. The as-prepared hybrid offers one three-dimensional architecture with the rich of open-porous structures. As the intimate chemical and electrical contact between the CuO and the His-GQDs was achieved, the hybrid of CuO with His-GQD creates a significant amplification of detection signal. The modified glass carbon electrode based on the CuO-His-GQD was used for differential pulse voltammetric detection of hydroquinone. Figures of merit include a wide linear response range (0.001–40 μM) and a 0.31 nM limit of detection at S/N ratio of 3. The sensor has been successfully applied in determination of hydroquinone in natural water samples. In addition, the study also provides an approach for fabrication of metal oxide-based nanocomposites with excellent electrochemical performance for sensing, catalysis, supercapacitors and lithium ion batteries.

Abnormal Anionic Porphyrin Sensing Effect for HER2 Gene Related DNA Detection via Impedance Difference between MWCNTs and Single-Stranded DNA or Double-Stranded DNA

Human epidermal growth factor receptor 2 (HER2) is a key tumor marker for several common and deadly cancers. It is of great importance to develop efficient detection methods for its over-expression. In this work, an electrochemical impedance spectroscopy (EIS) method adjustable by anionic porphyrin for HER2 gene detection has been proposed, based on the impedance difference between multi-walled carbon nanotubes (MWCNTs) and DNA. The interesting finding herein is that with the addition of anionic porphyrin, i.e., meso-tetra(4-sulfophenyl)-porphyrin (TSPP), the impedance value obtained at a glass carbon electrode (GCE) modified with MWCNTs and a single stranded DNA (ssDNA), the probe DNA that might be assembled tightly onto MWCNTs through π-π stacking interaction, gets a slight decrease; however, the impedance value from a GCE modified with MWCNTs and a double stranded DNA (dsDNA), the hybrid of the probe DNA with a target DNA, which might be assembled loosely onto MWCNTs for the screening effect of phosphate backbones in dsDNA, gets an obvious decrease. The reason may be that on the one hand, being rich in negative sulfonate groups, TSPP will try to push DNA far away from CNTs surface due to its strong electrostatic repulsion towards DNA; on the other hand, rich in planar phenyl or pyrrole rings, TSPP will compete with DNA for the surface of CNTs since it can also be assembled onto CNTs through conjugative interactions. In this way, the “loosely assembled” dsDNA will be repelled by this anionic porphyrin and released off CNTs surface much more than the “tightly assembled” ssDNA, leading to a bigger difference in the impedance value between dsDNA and ssDNA. Thus, through the amplification effect of TSPP on the impedance difference, the perfectly matched target DNA could be easily determined by EIS without any label. Under the optimized experimental conditions, this electrochemical sensor shows an excellent linear response to target DNA in a concentration range of 2.0 × 10−11–2.0 × 10−6 M with a limit of detection (LOD) of 6.34 × 10−11 M (S/N = 3). This abnormally sensitive electrochemical sensing performance resulting from anionic porphyrin for DNA sequences specific to HER2 gene will offer considerable promise for tumor diagnosis and treatment.

Ultrasensitive Electrochemical Sensor Based on Polyelectrolyte Composite Film Decorated Glassy Carbon Electrode for Detection of Nitrite in Curing Food at Sub-Micromolar Level.

To ensure food quality and safety, developing cost-effective, rapid and precision analytical techniques for quantitative detection of nitrite is highly desirable. Herein, a novel electrochemical sensor based on the sodium cellulose sulfate/poly (dimethyl diallyl ammonium chloride) (NaCS/PDMDAAC) composite film modified glass carbon electrode (NaCS/PDMDAAC/GCE) was proposed toward the detection of nitrite at sub-micromolar level, aiming to make full use of the inherent properties of individual component (biocompatible, low cost, good electrical conductivity for PDMDAAC; non-toxic, abundant raw materials, good film forming ability for NaCS) and synergistic enhancement effect. The NaCS/PDMDAAC/GCE was fabricated by a simple drop-casting method. Electrochemical behaviors of nitrite at NaCS/PDMDAAC/GCE were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Under optimum conditions, the NaCS/PDMDAAC/GCE exhibits a wide linear response region of 4.0 × 10−8 mol·L−1~1.5 × 10−4 mol·L−1 and a low detection 1imit of 43 nmol·L−1. The NaCS/PDMDAAC shows a synergetic enhancement effect toward the oxidation of nitrite, and the sensing performance is much better than the previous reports. Moreover, the NaCS/PDMDAAC also shows good stability and reproducibility. The NaCS/PDMDAAC/GCE was successfully applied to the determination of nitrite in ham sausage with satisfactory results.