Mosques have always been sacred places with distinctive sustainable environments. Fenestration in the prayers’ zone whether clerestories, screened windows, dome lighting and other light features have managed to produce significant spiritual human comfort areas. This paper focuses on fenestration of divine mosques and relates them to promoting daylight performance. The research process emphasizes the importance of daylight performance by promoting simulation tools on historical mosques of Alexandria since the 19th century that has witnessed change over time. The paper is a step toward sustainable lighting schemes in prayers’ zones that help to achieve human comfort as well as minimize use of energy. This study aimed at investigating the daylight performance by the use of climate based daylighting metrics which is “Daylight Autonomy” (DA). Daylight Autonomy is evaluated in the year round for the day lighted prayer periods to evaluate the behavior of fenestration of the different selected sample of mosques since the 19th century in Alexandria on a simulation tool in order to check whether it complies with the required illuminate and glare levels. The research findings are an attempt to lead to performative design guidelines introducing a contemporary interpretation for use in enhancing new designs of these holistic buildings.