This study inspects the concentrations of fine particulate matter (PM2.5) mass and carbonaceous species, including organic carbon (OC) and elemental carbon (EC), as well as their thermal fractions in the Indian Himalayan glacier region at the western Himalayan region (WHR; Thajiwas glacier, 2799 m asl), central Himalayan region (CHR; Gomukh glacier, 3415 m asl), and eastern Himalayan region (EHR; Zemu glacier, 2700 m asl) sites, throughout the summer and winter periods of 2019–2020. Ambient PM2.5 samples were collected on quartz fiber filters using a low-volume sampler, followed by carbon (OC and EC) quantification using the IMPROVE_A thermal/optical reflectance methodology. Different seasonal variations in PM2.5 and carbonaceous species levels were found at all three sites investigated. Averaged PM2.5 mass ranged 55–87 μg m–3 with a mean of 55.45 ± 16.30 μg m–3 at WHR, 86.80 ± 35.73 μg m–3 at CHR, and 72.61 ± 24.45 μg m–3 at EHR. Among the eight carbon fractions, high-temperature OC4 (evolved at 580 °C in the helium atmosphere) was the most prevalent carbon fraction, followed by low-temperature OC2 (280 °C) and EC1 (580 °C at 2% oxygen and 98% helium). Char-EC representing incomplete combustion contributed to 56, 67, and 53% of total EC, whereas soot-EC contributed to 38, 26, and 43% of total EC in WHR, CHR, and EHR, respectively. The measured OC/EC ratios imply the presence of secondary organic carbon, whereas char-EC/soot-EC ratios suggested that biomass burning could be the predominant source of carbon at CHR, whereas coal combustion and vehicular emission might be dominant sources at WHR and EHR sites.