The present study aims to estimate Above-Ground Biomass (AGB) in the moist deciduous forests of Mundgod Taluk, Uttara Kannada district, Karnataka, India using a combination of field sampling and remote sensing techniques. AGB was assessed using Sentinel-2A satellite imagery with a spatial resolution of 10 meters. Field data were collected using the Point-Centered Quarter (PCQ) method, it includes measuring tree girth at breast height (GBH), tree height, and canopy density. AGB was calculated using artificial form factor and specific gravity of tree species and further modelled using the Normalized Difference Vegetation Index (NDVI) derived from satellite data. The results showed a significant correlation between canopy density and biomass, with very dense forests exhibiting the highest AGB values. The field-measured AGB was 436.96 t/ha for very dense forests, 259.89 t/ha for moderately dense forests, and 161.67 t/ha for open forests. A linear regression model developed between AGB and NDVI showed a high R² value of 0.91 for open forests, 0.87 for moderately dense forests, and 0.85 for very dense forests. The total area-weighted biomass for the study area was estimated at 8.87 million tons, with 4.79 million tons in very dense forests, 3.44 million tons in moderately dense forests, and 0.76 million tons in open forests. The regression model validation indicated low Root Mean Square Error (RMSE) values, with the moderately dense forests 8.81 t/ha and the in very dense forests 7.54 t/ha. This study highlights the effectiveness of integrating field measurements with remote sensing for rapid, reliable AGB estimation. The approach offers valuable insights into biomass distribution and carbon sequestration potential, providing a scalable method for carbon inventories at state and national levels, contributing to forest management and climate change mitigation efforts.