Ginger Flower Research Articles

Activity of mosquito coils from the solid waste of patchouli (pogostemon cablin benth) with addition of torch ginger (etlingera elatior) flower

Activity of mosquito coils from the solid waste of patchouli (pogostemon cablin benth) with addition of torch ginger (etlingera elatior) flower

Investigation of physical properties, antioxidant activity, and sensory attributes of cocoa drink with the addition of torch ginger flower (Etlingera elatior) and xanthan gum

Indonesia is one of top cacao beans producer globally. Other than chocolate, cocoa drink is one of the popular cocoa derivates. The cocoa beverage is usually produced by mixing ingredients such as cocoa powder, sugar, creamer, and stabilizer. Cocoa powder is a great source of antioxidant as it contains polyphenolic compounds. However, the bioactive compounds like polyphenols might decrease during the processing of cocoa drinks. This study aimed to investigate the effect of torch ginger or kecombrang (Etlingera elatior) and xanthan gum addition on the physical properties, antioxidant activity, and sensory attributes of cocoa drink. The addition of torch ginger flower powder increased the antioxidant activity of the cocoa drink, but decrease the pH of the cocoa drink and consumers’ acceptance of aroma and taste of the cocoa drink. The addition of xanthan gum at 0.09 g resulted in the increased total solubility and reduced the sedimentation of cocoa drink.

Supplementation of Torch Ginger (Etlingera elatior) Flowers Improves the Quality and Safety of Traditional Fermented Shrimp Paste

Terasi, a traditional fermented shrimp paste from Indonesia, is commonly made from salted planktonic shrimp (Acetes japonicus) undergoing spontaneous lactic acid fermentation. In spite of its broad use and appreciated distinctive flavor, consuming terasi could be injurious for human consumption due to the presence of toxic compounds that are related to its deterioration and quality decrease, such as the allergenic histamine and probably carcinogenic acrylamide. These compounds are formed during fermentation of terasi due to a plethora of chemical reactions and microbial activities. This study aimed to improve the quality and safety of shrimp paste by incorporating during its fermentation torch ginger (Etlingera elatior) flowers, locally known as bunga kecombrang, that are rich in antioxidants and antimicrobial compounds. These flowers are widely utilized in Indonesian cuisine. The supplementation of torch ginger flowers (5%(w/w) and 10% (w/w)) significantly reduced microbial growth, lipid peroxidation, as well as the formation of histamine and acrylamide in terasi fermented over a period of 30 days. In addition, the supplementation of 10% torch ginger flowers improved the sensory acceptance of terasi. Therefore, this study suggests the potential of torch ginger flowers as an additional ingredient to improve the safety and quality of terasi.

Tea Fungus Beverages from Torch Ginger (Etlingera elatior): Total microbial, Physicochemical, and Antioxidant Activity

Functional beverages are needed to maintain health and fitness as a part of the instant lifestyle and degenerative diseases. The scientific findings on torch ginger (Etlingera elatior) flower (TGF) as a tea fungus beverage (Kombucha) are still limited. This research evaluated total microbes, physicochemical properties, and antioxidant activity 7 and 14 days after fermentation (daf) of TGF kombuchas. In addition, TGF in variant 0% (TGF0), 5% (TGF5), 10% (TGF10), and 15% (TGF15) are fermented with 3% of SCOBY, 20% culture, 1% green tea, and 10% sucrose. The antioxidant activities of TGF kombuchas were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azino-bis 3-Ethylbenzothiazoline-6-Sulfonic Acid (ABTS) assay—the phenolic and flavonoid content using Folin-Ciocalteu method-gallic acid equivalent, and quercetin acid equivalent, respectively. The result showed that the fermentation time (p0.05) was affected by the properties of TGF kombuchas. Total microbes, physicochemical properties (pH, acidity, cellulose pellicle weight, phenolic, flavonoid content), and antioxidant activity were higher in 14 than in 7 days. Total soluble solid and total yeast count showed lower values in 14 days. The increase in total bacteria count, TGF15%, led to the highest increasing density from 8.08 ± 0.02Log CFU/mL on seven daf to 13.34 ± 0.04Log CFU/mL on 14 daf. The TGF 10% and 15% kombuchas in 14 dafs showed abundance in phenolic and flavonoid content, 121.45±1.07mg GAE/mL and 1.70±0.04 mg QAE/mL, respectively. During 14 daf and TGF 10%, the Kombucha of torch ginger flower demonstrated high antioxidant activity at 85.92±0.07% DDPH and 63.05±0.97ABTS. It is expected to aid future research into developing functional kombucha beverages.

The Activities of Torch Ginger Flower (Etlingera elatior) Ethanol Extract on Degradation of Porphyromonas gingivalis Biofilm as Periodontal Pathogen


 Introduction: Porphyromonas gingivalis is bacteria that can form biofilms as the main cause of periodontitis. Mouthwash therapy in long term can cause mucositis and even oral cancer. Antibacterial potential of torch ginger flower (Etlingera elatior) can be developed as an alternative adjuvant therapy for periodontitis. Objective: Aims of this research was to determine the effect of torch ginger flower ethanol extract against degradation of P. gingivalis biofilm. Methods: This research used ethanolic extract of torch ginger flower with concentrations 1.56 mg/mL, 3.125 mg/mL, 6.25 mg/mL, 12.5 mg/mL, 25 mg/mL, and 50 mg/mL. Chlorhexidine gluconate 0.2% was used as positive control and DMSO 1% was used as negative control. Measurement of P. gingivalis biofilm degradation used microtiter plate assay with crystal violet 1% staining which reads its optical density at wavelength of 450 nm. Data were analyzed by one way ANOVA and Post hoc LSD. Results: The percentage of P. gingivalis biofilm degradation with torch ginger flower ethanol extract sequentially were 12.47%, 30.56%, 57.12%, 71.36%, and 74.83%. The analysis showed that there was a significant difference (p<0,05) between treatment groups torch ginger flower ethanol extract, as well as between torch ginger flower ethanol extract with DMSO 1% and chlorhexidine gluconate 0.2%. Optimum concentration of ethanol extract of torch ginger flower on P. gingivalis biofilm degradation was 25 mg/mL and showed no significant difference with chlorhexidine gluconate 0,2% (p>0,05). Conclusion: Conclusion of this research is torch ginger flower (Etlingera elatior) ethanol extract has P. gingivalis biofilm degradation activity.biofilm

Exploring the impact of dry conditioning on the postharvest quality and longevity of torch ginger flower stems

Exploring the impact of dry conditioning on the postharvest quality and longevity of torch ginger flower stems

Uji Efektivitas Antibakteri Ekstrak Etanol Bunga Kecombrang (Etlingera elatior) Terhadap Pertumbuhan Klebsiella pneumoniae

Ginger flower (Etlingera elatior) is an herbal plant that has secondary metabolite compounds that function as antibacterial. Klebsiella pneumoniae bacteria is the cause of penumoniae, urinary tract infections and nosocomial infections. The purpose of this study was to determine the effect of the ethanolic exstract of Etlingera elatior in hibiting the growth of Klebsiella pneumoniae. Work procedure was started extracting ginger flower with 96% ethanol solvent, phythochemical screening test and effectiviness test with Kirby bauwer diffusion method and exstract concentration groups of 10%, 20%, 30%, 40% and 50% as well as positive control of meropenem antibiotics and negative control of distilled water. Data analysis used SPSS 25 with one way ANOVA test and Duncan’s follow-up test. The results showed that the ethanolic exstract of ginger flower was effective in inhibiting the growth of Klebsiella pneumoniae bacteria at concentrations of 30%, 40% and 50%, respectively, with an averange inhibition zone diameter of 14 mm, 15,2 mm and 15,6 mm.

PENINGKATAN KUALITAS COOKIES DENGAN PENAMBAHAN MINYAK ATSIRI BUNGA KECOMBRANG (Etlingera elatior)

Antioxidants are compounds that can inhibit or slow down oxidative damage due to free radicals. Torch ginger flower (also known as kecombrang flower in Indonesia) essential oil has antioxidant activity values ranging from 61.61-83.17%. This study aim to determine the best concentration of torch ginger flower essential oil (Etlingera elatior) in cookies and to improve the chemical and microbiological qualities of cookies. Torch ginger flowers are cut into pieces and then distilled. The stage of adding torch ginger flowers essential oil was carried out according to the experimental design namely P (100: 0), Q (100: 0.1), R (100: 0.2), S (100: 0.3). The observation parameters in this study were proximate, antioxidant activity, and microbiological (total plate count) parameters. Data processing was carried out in a completely randomized design (CRD) based on data from observations of the test parameters with three repetitions. The result of study showed that the cookies with the addition of torch ginger flower essential oil had moisture content of 4.50-5.11%, ash content of 0.80-1.05%, fat content of 20.68-22.45%, protein content of 6.88-7.95%, carbohydrate content of 63.85-65.30%, antioxidant activity of 55.45-70.78%, the total plate count (bacteria, mold, and yeast) about <10 colonies/g.
 Keywords: antioxidants, cookies, torch ginger flower

GC-MS Based Metabolite Profiling and Antibacterial Activity of Torch Ginger (<i>Etlingera elatior</i>) Flowers Extract

Torch ginger (Etlingera elatior) flowers are well known for their antibacterial effects against Staphylococcus aureus, however, the active compounds are still unknown. The purpose of this study was to conduct GC-MS-based metabolite profiling of torch ginger flower and identify compounds correlated with its S. aureus antibacterial activity using Orthogonal Projection to Latent Structure (OPLS). Using the well diffusion method, the antibacterial activity of ethanol extract, hexane, chloroform, and ethyl acetate fractions with a concentration of 80 mg/mL were investigated. The ethyl acetate fraction inhibited S. aureus growth the most (diameter of inhibition zone, DIZ 13.00–13.20 mm), while the hexane (DIZ 9.55–10.05 mm) and chloroform (DIZ 10.00–11.00 mm) fractions had moderate inhibitory activity, but the ethanol extract had no antibacterial effect. Using OPLS analysis, the GC-MS metabolite profile of all extracts and fractions was linked with the profile of antibacterial activity. This analysis revealed that Dodecanoic acid, 5-Tetradecene, and n-Hexadecanoic acid were identified as the compounds that were significantly connected with antibacterial activity.

First report on pollinating behavior of the small carpenter bee Ceratina ridleyi Cockerell (Hymenoptera, Apidae) in Globba leucantha var. bicolor Holttum (Zingiberaceae)

Small carpenter bees of the genus Ceratina are widespread, but little-studied as pollinators despite their presence in various pollinator assemblages. This study documents the importance of Ceratina ridleyi as a pollinator of the small-flowered Malaysian ginger Globba leucantha var. bicolor. Species of Zingiberaceae are defined by peculiar floral morphologies (e.g staminodes and a single functional anther) but are rarely studied for their insect pollinators. Surprisingly, C. ridleyi was revealed as the single pollinator of Globba leucantha’s showy flowers even though the presence of Apis sp., Trigona sp., and Amegilla sp. were observed in the study site. This small carpenter bee collects both pollen and nectar from the ginger flower. Pollen grains were observed attached to its scopal hairs and hairs on the ventral thorax and ventral abdomen. The bee contacts the flower’s sole stigma only during foraging for pollen, where the stigma contacts pollen accumulated on hairs of the bee’s ventral thorax and ventral abdomen. This study represents the first pollination report of a Ceratina pollinating a species of Zingiberaceae, and only the second report of any Ceratina pollinating a flower with complex morphology.

Inheritance form of ginger flowers in sunflower (Heliantus annuus L.)

Using hybridological and isogenic analyzes, the allelicity of genes controlling the shape of reed flowers in four nearly isogenic lines of sunflower was studied. It was found out that the recessive alleles fs and fn, which control short and narrow ligulate flowers, respectively, belong to different loci, and the fs and fn alleles interact with each other in a complementary manner. The recessive alleles fn and ftw, which control narrow and curled reed flowers, respectively, belong to different loci, and the fs and ftw alleles interact with each other in a complementary manner. The recessive alleles fn and ft controlling, respectively, narrow and tubular reed flowers belong to different loci, and the fn and ft alleles interact with each other in a complementary manner. These recessive genes can be used to breed new varieties and hybrids of ornamental sunflower, as well as to mark varieties and hybrids of oilseed, roasted, confectionery and silage sunflowers.

The prophylactic potential of Zingiber officinale flowers and leaves extract to mitigate hyperglycemia in Sprague Dawley rats

PurposeInnovative health-promoting approaches of the era have verified phytoceutics as one of the prime therapeutic tools to alleviate numerous health-related ailments. The purpose of this paper is to probe the nutraceutic potential of ginger flowers and leaves against hyperglycemia.Design/methodology/approachThe aqueous extracts of ginger flowers and leaves were observed on Sprague Dawley rats for 8 weeks. Two parallel studies were carried out based on dietary regimes: control and hyperglycemic diets. At the end of the experimental modus, the overnight fed rats were killed to determine the concentration of glucose and insulin in serum. The insulin resistance and insulin secretions were also calculated by formulae by considering fasting glucose and fasting insulin concentrations. Furthermore, the feed and drink intakes, body weight gain and hematological analysis were also carried out.FindingsIn streptozotocin-induced hyperglycemic rats, the ginger flowers extract depicted 5.62% reduction; however, ginger leaves extract reduced the glucose concentration up to 7.11% (p = 0.001). Similarly, ginger flowers extract uplifted the insulin concentration up to 3.07%, while, by ginger leaves extract, the insulin value increased to 4.11% (p = 0.002). For the insulin resistance, the ginger flower showed 5.32% decrease; however, the insulin resistance was reduced to 6.48% by ginger leaves (p = 0.014). Moreover, the insulin secretion increased to 18.9% by flower extract and 21.8% by ginger leave extract (p = 0.001). The feed intake and body weight gain increased momentously by the addition of ginger flowers and leaves; however, the drink intake and hematological analysis remained non-significant by the addition of ginger parts.Originality/valueConclusively, it was revealed that leaves have more hypoglycemic potential as compared to flowers.

Production performance and meat quality of local ducks fed rations containing extract of torch ginger (Etlingera elatior) flowers and betel (Piper Betle linn) leaves

Compared to chickens, ducks still have low productivity and other limitations. One of the causes of these problems might be attributed to the off-odor found in duck meat. Therefore, production performance and meat quality of ducks need to be improved. The inclusion of extracts of betel (Piper betle Linn) leaves and torch ginger (Etlingera elatior) flowers wereas done to improve production performance and meat quality of local ducks. This study was aimed at assessing the effects of the inclusion of betel leaves and torch ginger flowers in the basal rations on production performance and meat quality of local ducks in the growing phase. A completely randomized factorial design in 4 x 4 with three replicates was used. The first factor consisted of four levels of torch ginger flower solution, namely 0, 2.5, 5, and 7.5%. The second factor consisted of four levels of betel leaf solution, namely 0, 2.5, 5, and 7.5%. Results showed that no significant differences (P>0.05) were found in body weight gain, feed intake, feed conversion, mortality rate and meat quality (pH, water holding capacity, tenderness and cooking loss). It was concluded that the inclusion of extract of betel leaves and torch ginger flowers did not improve production performance and meat quality of male local ducks.

Aspects of the reproductive biology of Zingiber spectabile (Zingiberaceae)

Ginger (Zingiber spectabile) is cultivated worldwide as a floriculture and landscaping species. Studies on the biology and floral morphology of this species are important for its improvement; however, they are scarce. We investigated the reproductive biology and floral morphology of gingers grown in a germplasm bank. Floral development was monitored during the pre-anthesis and anthesis periods. Floral morphometry, pollen viability, stigmatic receptivity, volume and concentration of nectar sugar, and performance of possible pollinators were evaluated. Ginger flowers had a 24-h durability. Ginger torch is an allogamous species that exhibits floral hercogamy like other Zingiberaceae. Ginger flowers had a 12-h anthesis. During anthesis, floral structures related to reproduction such as the style, stigma, and anther crest continued to develop, increasing floral herkogamy. Pollen viability was above 70% and the stigma remained receptive throughout the anthesis period. Hummingbirds were the only pollinators recorded in the study area that were able to move the anther crest. Fruiting was determined in a natural pollination system. The occurrence of nocturnal anthesis characterized by an increase in the development of reproductive structures and nectar production suggests that the species is adapted to nocturnal visitors.

Development of Etlingera elatior pearl for drinks and cocktail

Herbs such as Etlingera elatior (torch ginger flower) is well known in Malaysia and one of the main sources of phytochemicals with strong antioxidant activity. A recent study is done to develop herbs into new confectionery products, pearls in drinks and cocktails. In this research, the screening process is done to select the best pearl formulation, which described the desired viscosity and pH. The mixture variables are different types of starch (xanthan gum, modified cassava flour, hanjeli flour, and modified suweg flour). Five out of 20 pearl formulations are chosen for texture profile, antioxidant analysis, and sensory analysis. The result shows pearl formulation 2 (467.62 g force) has the highest hardness of the pearl, while the stickiest is pearl formulation 4 (-9.24 g force). The pearl formulation 2 has the highest antioxidant capacity when tested with TPC (6.61 μgGAE/ml), TFC (177.42 μgRE/ml) and ABTS (56.74 %). Contradict to this result, the highest DPPH antioxidant properties are pearl formulation 1 (69.98 %). The sensory analysis by 9-point hedonic scale result shows that the average mean of overall acceptance is formulation 2.

ANTI-MICROBIAL ACTIVITY OF GINGER FLOWER AGAINST SOME CAUSATIVE AGENT OF ACUTE RESPIRATORY INFECTION

Various microorganisms such as Streptococcus pneumonia, Haemophilus influenza, Chlamydia spp, and Mycoplasma pneumonia can cause ARI. The ginger flower which contains various phytochemicals that have potential anti-microbial. Recent study was aimed to explore the anti-microbial properties of ginger flower extract. This study was experimental in vitro study with the disc diffusion method. The ethanol extract of Ginger flowers was obtained by maceration method with a ratio of Simplicia to solvent 1:10. Then it concentrated with a rotary evaporator. The ethanol extract of the ginger flower was diluted by DMSO into four concentrations viz. 100%, 75%, 50%, and 25%. The anti-microbial activity assay used three different types of microbes (Streptococcus pneumonia, Haemophilus influenza, and Candida albicans) different in 3 repetitions. There were significant differences each concentration on the width of clear zone among Streptococcus pneumonia (P-value = 0.010), Haemophilus influenza (P-value = 0.011), and Candida albicans (P-value = 0.005). Also, all concentrations of ginger flower extract showed no significant difference between positive controls; however, significant differences were found for negative controls. Hence, It can be concluded that 25% of ginger flower extract has been sufficient to inhibit the growth of various tested microorganisms.

FORMULASI SEDIAAN MASKER WAJAH MENGGUNAKAN EKSTRAK ETANOL BUNGA KECOMBRANG (ETLINGERA ELATIOR) TERHADAP KELEMBABAN KULIT WAJAH

ABSTRACT
 
 
 Skin is an essential and vital organ and a reflection of health. Natural antioxidants such as flavonoids, polyphenols and Sapponin are believed to increase moisture on the skin. This ability is the main reason for using Torch Ginger flower extract which is formulated into a form of facial mask in the form of clay facial mask. Objective: To formulate facial moisturizing mask preparations using the extract of Torch Ginger(Etlingera elatior). This study used the Torch Ginger Flower extract facial moisturizing mask formulation method with a concentration of 0 (Blank), 5% and 10%. Evaluation of mask preparations included homogeneity, stability of dosage at temperatures of 80C and 250C, Organoleptic test and pH of preparations. Measurements of skin moisture activity were measured using alar Skin Analyzer which was tested on the face surface 3cm wide. The results showed that the preparation was stable in terms of odor, color and was at a pH of 4.2-6.3 using observations of litmus discoloration. Effectiveness of facial skin moisture with 10% Torch Ginger extract concentration is more effective to produce results at a concentration of 5% and blank. Conclusion: Torch Ginger flower extract can be formulated into preparations of face masks with various concentrations and has the effect of moisturizing the skin and brightening the complexion.
 Keywords: Torch Ginger Flower Extract, Facial Mask, Clay Facial Mask, Facial Skin Moisture

Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts

BackgroundIn the present era, the attention of nutritionist diverted towards the bioactive entities present in natural sources owing to the presence of health boosting perspectives against lifestyle related disarrays.MethodsIn this context, different parts of ginger crop i.e. rhizome, leaves and flower of variety Suravi (ID no. 008) were used for the preparation of ginger extracts with 50% methanol, 50% ethanol and water via rotatory shaker for 45 min. After that, different phytochemical analysis and in vitro analyses were carried out to determine the antioxidant potential of these extracts. Lastly, the best selected extracts from each part was quantified through HPLC.ResultsThe results of current investigated indicated that ethanol extract proved to have maximum quantity of phytoceutics as compared to methanol and water. The maximum TPC, flavonoids, flavonols, DPPH assay, antioxidant activity, FRAP assay, ABTS assay and metal chelating potential was observed in ginger leaves as 780.56 ± 32.78 GAE/100 g, 253.56 ± 10.65 mg/100 g, 49.54 ± 1.74 mg/100 g, 75.54 ± 3.17%, 77.88 ± 3.27%, 105.72 ± 4.44 μmole TE/g, 118.43 ± 4.97 μmole TE/g and 35.16 ± 1.48%, respectively followed by ginger flowers and ginger rhizome. The lowest antioxidant activity was estimated in ginger rhizome. On the basis of phytochemical profiling and in vitro analyses, ethanol extracts of ginger flowers, leaves and rhizome were selected for the quantification through HPLC.ConclusionThe findings proved that maximum 6-gingerol was present in ginger leaves (4.9 mg/g) tackled by ginger flowers (2.87 mg/g) and ginger rhizome (1.03 mg/g).

Antibacterial Activity and Metabolomics Profiling of Torch Ginger (Etlingera elatior Jack) Flower Oil Extracted Using Subcritical Carbon Dioxide (CO2)

The aim of this study was to identify the bioactive compound and evaluate the antibacterial activity of torch ginger flower oil extracted using subcritical carbon dioxide. The antibacterial activity was evaluated in agar diffusion assay, while MIC and MBC were determined using the microdilution broth assay. The essential oil was subjected to metabolomics profiling using GC‐MS and 1H‐NMR techniques. The results demonstrated strong antibacterial activity towards Salmonella typhimurium, Staphylococcus aureus, and Escherichia coli. The MIC values were 0.0625, 0.25, and 0.25 mg/mL, and the MBC values were 0.25, 0.5, and 1 mg/mL towards S. typhimurium, S. aureus, and E. coli, respectively. A total of 33 compounds were identified using GC‐MS including 15 compounds (45%) known for their antimicrobial activity. In addition, sixteen metabolites were identified using NMR analysis and 8 out of the sixteen metabolites (50%) have antibacterial activity. The extracted oil demonstrated broad range for antibacterial activity and has high potential for applications in pharmaceutical and food industries. Practical Applications. The oil extracted from the torch ginger flower was found very stable and has promising applications as antibacterial agent for food and pharmaceutical industries.

Anatomical aspects of the ginger flower Etlingera elatior (Jack.) RM Smith, acclimatization with the usage of different nutritive solutions

The Ginger flower, Etlingera elatior (Jack.) RM Smith (also known as torch ginger, ginger flower, red ginger lily, torch lily, wild ginger), belonging to the Zingiberaceae family, stands out with one of the main ornamental tropical species. The objectives of the present study were to establish an acclimatization methodology for in vitro cultivated cane seedlings, and to compare the internal structure of leaves of seedlings submitted to different nutrient solutions. The seedlings from the in vitro culture were transplanted into trays with inert substrate (expanded vermiculite + 10% sand), where they remained in a growth room with controlled temperature of 25 ± 2 °C and photon irradiance of 67 μm m-2 s-1. Moisture control was carried out by using a transparent plastic bag, which was removed after 15 days and shortly thereafter, the experiments were started with the use of nutritive solutions MS (Murashige & Skoog), BJ (Bolle-Jones) and HO (Hoagland & Arnon) at 35% of their ionic strength. For the anatomical study, transverse and paradermal sections were realized in the foliar laminae of seedlings in vitro, in period of acclimatization and in plants already established. The in vitro seedlings showed survival at 100% when transferred to the ex vitro condition. In the cross sections and leaf parser, anatomical differences between the seedlings of the different environments were observed. The abaxial and adaxial epidermis presented significant differences in the environments in which the seedlings developed, showing themselves to be thicker as the acclimatization period increased. The thickness averages of the palisade parenchyma did not differ statistically in the acclimatization periods and in the different nutrient solutions tested. On the other hand, those ones of the spongy parenchyma´s presented significant differences in relation to the nutrient solutions tested, evidencing in all evaluations the MS solution, which contributed the most to the development of the internal structures of the leaf (spongy parenchyma and central vascular cylinder). The highest stomatal density was observed in seedlings developed in vitro (250 stomata per mm2) when compared to those ones already acclimatized or even to that one of field, 200 and 190 stomata per mm2 respectively. The stomata of the Ginger flower leaves developed in vitro presented with polar and equatorial diameters greater than the stomata of leaves already acclimatized and as well as of field.