Fusarium Lateritium Research Articles

Fungi Detected in the Previous Year’s Leaf Petioles of Fraxinus excelsior and Their Antagonistic Potential against Hymenoscyphus fraxineus

Studies on fungal communities in the previous year’s leaf petioles of Fraxinus excelsior found in litter in five ash stands in southern Poland were made in 2017. Fungi were identified on the basis of isolation from 300 surface sterilized leaf petioles and by in situ inventory of fruit bodies (on 600 petioles, in spring and autumn). Identification was based on morphology of colonies and fruit bodies, and sequencing of ITS region of the rRNA gene cluster. In total, 2832 isolates from 117 taxa (Ascomycota—100; Basidiomycota—15; Mucoromycota—2 taxa) were obtained with the isolation method. The most frequent taxa (with frequency >10%) were: Nemania serpens, Hymenoscyphus fraxineus, Alternaria sp. 1, Boeremia sp., Helotiales sp. 1, Epicoccum nigrum, Venturia fraxini, Fusarium sp., Fusarium lateritium, Nemania diffusa, Typhula sp. 2 (in descending order). In total, 45 taxa were detected with the in situ inventory method. Eleven taxa were classified as dominant: Hymenoscyphus fraxineus, Venturia fraxini, Leptosphaeria sp. 2, Cyathicula fraxinophila, Typhula sp. 2, Hypoderma rubi, Pyrenopeziza petiolaris, Cyathicula coronata, Hymenoscyphus scutula, Leptosphaeria sclerotioides and Hymenoscyphus caudatus. Among 202 leaf petioles colonized by H. fraxineus, 177 petioles also showed fructification of 26 other fungi. All the isolated saprotrophs were tested in dual-culture assay for antagonism to two strains of H. fraxineus. Three interaction types were observed: type A, mutual direct contact, when the two fungi meet along the contact line (occurred with 43.3% of test fungi); type B, with inhibition zone between colonies (with 46.9% of test fungi); type C, when the test fungus overgrows the colony of H. fraxineus (with 9.8% of test fungi). The possible contribution of the fungal saprotrophs in limiting of the expansion of H. fraxineus in ash leaf petioles, which may result in reduction in the inoculum of ash dieback causal agent, is discussed.

4,6'-Anhydrooxysporidinone from Fusarium lateritium SSF2 Induces Autophagic and Apoptosis Cell Death in MCF-7 Breast Cancer Cells.

Previous studies have reported that 4,6′-Anhydrooxysporidinone (SSF2-2), isolated from Fusarium lateritium SSF2, has neuroprotective effects on the HT-22 hippocampal neuronal cell line. However, the anti-cancer effect of SSF2-2 remains unclear. Here, we examined the viability of MCF-7 human breast cancer cells treated with SSF2-2 or left untreated using a cell viability assay kit. The underlying molecular mechanism was further investigated by Western blotting and immunocytochemistry studies. The results demonstrated that SSF2-2 inhibited the viability of MCF-7 cells. Treatment with SSF2-2 increased the levels of cleaved caspase-9, cleaved caspase-7, poly (ADP-ribose) polymerase (PARP), and LC3B. Additionally, SSF2-2 significantly increased the conversion of LC3-I to LC3II and LC3-positive puncta in MCF-7 cells.

Fusarium Species in Mangrove Soil in Northern Peninsular Malaysia and the Soil Physico-Chemical Properties.

Fusarium genus comprises important saprophytic and phytopathogenic fungi and is widespread in nature. The present study reports the occurrence of Fusarium spp. in soils from two mangrove forests in northern Peninsular Malaysia and analyzed physico-chemical properties of the mangrove soil. Based on TEF-1α sequences, nine Fusarium species were identified: Fusarium solani species complex (FSSC) (n = 77), Fusarium verticillioides (n = 20), Fusarium incarnatum (n = 10), Fusarium proliferatum (n = 7), Fusarium lateritium (n = 4), Fusarium oxysporum (n = 3), Fusarium rigidiuscula (n = 2), Fusarium chlamydosporum (n = 1), and Fusarium camptoceras (n = 1); FSSC isolates were the most prevalent. Phylogenetic analysis of the combined TEF-1α and ITS sequences revealed diverse phylogenetic affinities among the FSSC isolates and potentially new phylogenetic clades of FSSC. Soil analysis showed varied carbon content, pH, soil moisture, and salinity, but not nitrogen content, between sampling locations. Regardless of the physico-chemical properties, various Fusarium species were recovered from the mangrove soils. These were likely saprophytes; however, some were well-known plant pathogens and opportunistic human pathogens. Thus, mangrove soils might serve as inoculum sources for plant and human pathogenic Fusarium species. The present study demonstrates the occurrence of various Fusarium species in the extreme environment of mangrove soil, thereby contributing to the knowledge on species diversity in Fusarium.

Antifungal sesquiterpenes with post-harvest anthracnose control effect on bananas from the fungus Fusarium lateritium

To search for antifungal leads, the metabolites of an insect-derived fungus Fusarium lateritium ZMT01 were investigated, providing five sesquiterpenes (1–5), including new molecules microsphaeropsisins D and E (1 and 2). The evaluated antifungal activities in vitro which are higher than the positive control triadimefon include: 1 and 2 towards Fusarium oxysporum (MICs 50, 25 mg L−1; triadimefon 100 mg L−1); 1, 2, 4 and 5 towards Penicillium italicum (MICs 25, 12.5, 25, 25; triadimefon 50 mg L−1), 1, 2 and 4 towards Colletotrichum musae (MICs 25, 12.5, 25; triadimefon 80 mg L−1), 2 and 4 towards Fusarium graminearum (MICs 100, 100; triadimefon 150 mg L−1). The bioassay in vivo displayed that the banana anthracnose control effect of 2 (100 mg L−1) was also higher than that of triadimefon (Inhibition ratios 27.5 ± 2.5%, 55.3 ± 1.4%, 52.1 ± 1.3% for 2, 22.5 ± 2.1%, 47.2 ± 2.0%, 36.6 ± 2.2% for triadimefon at 4 d, 8 d and 12 d, respectively).

Neuroprotective Effect of Tricyclic Pyridine Alkaloids from Fusarium lateritium SSF2, against Glutamate-Induced Oxidative Stress and Apoptosis in the HT22 Hippocampal Neuronal Cell Line.

Excessive glutamate damages neuronal cells via the accumulation of intracellular reactive oxygen species (ROS), calcium ion (Ca2+) influx, depolarization of mitochondrial membrane potential, and apoptosis, which may result in the development of chronic neurodegenerative diseases. In this study, we evaluated the effects of 4,6′-anhydrooxysporidinone isolated from endophytic fungus Fusarium lateritium SSF2 on glutamate-induced cytotoxicity, accumulation of intracellular ROS, increases in superoxide anion production, Ca2+, depolarization of mitochondrial membrane potential, and apoptotic cell death in hippocampal HT22 cells. 2′,7′-Dichlorofluorescin diacetate (H2DCFDA) staining was used to determine the intracellular reactive oxygen species concentration and dihydroethidine (DHE) staining was used to determine the superoxide radical. Expression of the nuclear factor-erythroid-2–related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was analyzed by Western blot. Fluo-4 staining was used to determine the intracellular Ca2+ levels. In order to explore mitochondrial membrane potential, tetramethylrhodamine methyl ester (TMRM) staining was used. Apoptotic cell death was evaluated using Annexin-V/propidium iodide (PI) staining and TUNEL staining. Expression of the cytochrome c release and cleaved caspase-9, -3 was analyzed by Western blot. Here, we were able to isolate 4,6′-anhydrooxysporidinone from endophytic fungus, Fusarium lateritium SSF2, which was shown to protect HT22 cells from glutamate-induced cytotoxicity, accumulation of intracellular ROS, increases in superoxide anion production, Ca2+, and depolarization of mitochondrial membrane potential. In addition, 4,6′-anhydrooxysporidinone enhanced the expressions of Nrf2 and HO-1. It also inhibited the apoptotic cell death through the inhibition of cytochrome c release and cleaved caspase-9, -3 in glutamate-treated HT22 cells. Therefore, our results provide ample evidence of the neuroprotective properties of 4,6′-anhydrooxysporidinone.

Fusarium massalimae sp. nov. (F. lateritium species complex) occurs endophytically in leaves of Handroanthus chrysotrichus

Fusarium isolates obtained as endophytes from healthy leaves of Handroanthus chrysotrichus (Bignoniaceae) in the Atlantic Forest of north-eastern Brazil were characterised morphologically and phylogenetically on the basis of partial rDNA sequences encompassing the second largest subunit of RNA polymerase II, translation elongation factor EF-1 alpha, and β-tubulin genes. A novel species within the Fusarium lateritium species complex (FLSC) is described as Fusarium massalimae. Isolates of F. pseudocircinatum of the Fusarium fujikuroi species complex (FFSC) were also isolated as endophytes from leaves of H. chrysotrichus. Mating type gene analyses suggested that obtained isolates of F. massalimae and F. pseudocircinatum are heterothallic.

Состав энтомопатогенных видов гифальных грибов с пораженных гусениц хлопковой совки

В статье обсуждаются вопрос поиска энтомопатогенных грибов, выявленных с пораженных гусениц хлопковой совки на посевах хлопчатника и овощных культур. В результате выделено 20 видов энтомопатогенных гифальных микромицетов из 12 родов, 3 семейств грибов. Была проверена вирулентность выявленной микобиоты. Из представленных данных следует, что процент поражения гусениц восковой моли в опыте при искусственном заражении колеблется в пределах от 2 до 25%. Отмечено, что гибель гусениц в течение 20 дней отмечалась лишь в случае со штаммами видов Aspergillus fumigates (4% гусениц погибли), Aspergillus flavus, Trichotecium roseum, Spicaria heliothis и Fusarium lateritium вызвали гибель 2% гусениц. На основании полученных данных был сделан вывод, что выявленные виды в той или иной степени обладают определенными энтомопатогенными свойствами, но данные штаммы ввиду слабой вирулентности для насекомых не могут быть примененными в практических целях.

Synergistic effect of Fusarium lateritium LP7 and Trichoderma viride LP5 promotes ethoxylated oleyl-cetyl alcohol biodegradation

This study evaluated the growth and metabolic activity of consortium and pure cultures Fusarium lateritium LP7 and Trichoderma viride LP5 in response to the presence of 0.5% ethoxylated oleyl-cetyl alcohol (EOCA) in the liquid Czapek-Dox medium. The effectiveness of mentioned cultures was monitored according to the following parameters: biomass dry weight (BDW), pH, quantity of free and total organic acids, proteolytic activity and the qualitative composition of carbohydrates, during 19 days. The biodegrading efficiency was determined spectrophotometrically. The BDW of consortium was significantly stimulated by EOCA (16.59%) whereas biomass of LP7 was significantly inhibited (30.61%). The EOCA had influence on decrease in pH value of the media of LP5 and consortium, and pH changes were correlated with the amount of excreted organic acids. The alkaline protease activities of consortium, LP7 and LP5 retained 73%, 62.2% and 49.5% activity respectively in the presence of EOCA. Consortium has shown the best biodegradation capacity up to 82% of EOCA. The pure cultures were less effective in biodegradation and removed approximately 65% (LP7) and 60% (LP5) of EOCA after 19 days. In brief, the synergistic interaction between pure cultures enhances capacity to reduce EOCA in environment and influences production of some biotechnological useful metabolites.

Endophytic fungi from the medicinal herb Euphorbia geniculata as a potential source for bioactive metabolites.

Many researchers proved that plant endophytes manage successful issues to synthesize active chemicals within plant cells. These bioactive compounds might support a range of plant defense mechanism against many pathogenic microorganisms. In this study, a total of 22 isolates representing 21 fungal species belonging to 15 fungal genera in addition to one variety were isolated and identified for the first time from Euphorbia geniculate plants. The genus Aspergillus was the most common fungus isolated from the studied plant. The fungus Isaria feline was recorded in both leaves and stem, while Aspergillus flavus, A. ochraceus, A. terreus var. terreus, Emercilla nidulans var. acristata, Macrophomina phaseolina colonized both stem and root. The isolated fungi showed antagonistic activities against six strains of plant pathogenic fungi viz., Eupenicillium brefeldianum, Penicillium echinulatum, Alternaria phragmospora, Fusarium oxysporum, Fusarium verticilloid, and Alternaria alternata in dual culture assay. The highest antagonistic activity fungal species (Aspergillus flavus, A. fumigatus, and Fusarium lateritium) and the lowest (Cladosprium herbarum, F. culomrum, and Sporotrichum thermophile) showed twining in their secondary metabolites especially terpens and alkaloids with that of their host E. geniculata. Three concentrations of (0.5, 1.0, and 2.0mg/ml) of these secondary metabolites extracted by ethyl acetate and n-butanol from the above six endophytic fungal species were tested against three pathogenic fungi isolated from infected tomato plant (E. brefeldianum-EBT-1, P. echinulatum-PET-2, and A. phragmospora-APT-3), whereas these pathogens showed promising sensitivity to these fungal secondary metabolites. In conclusion, this is the first report on the isolation of endophytic fungi from E. geniculata and evaluation of their antifungal activity.

Allergenicity assessment of fungal species using immunoclinical and proteomic techniques: a study on Fusarium lateritium

ABSTRACT Airborne fungal spores are extensively reported as the elicitors of respiratory allergies in human. Fusarium lateritium is one such fungal species reported for eliciting significant skin prick results from India. The present study aims to analyze the allergenic potential of F. lateritium followed by the identification of allergens. The total protein of F. lateritium was subjected to 1dimensional (1D) and 2D gel electrophoresis followed by corresponding IgE-specific immunoblots. We found 8 immunoreactive bands/zones in (1D) immunoblot using 11 F. lateritium-sensitised patient sera. In 1D immunoblot, a 34 kDa band was detected in >80% of the patients and hence considered as a potential allergen of F. lateritium. Corresponding 34 kDa spot in 2D-immunoblot was analyzed by mass spectrometric analysis and identified as Glyceraldehyde 3-phosphate dehydrogenase. The identified F. lateritium allergen holds the potential to instigate vaccine development for immunotherapy of F. lateritium sensitized patients.

First report of Fusarium lateritium causing fruit rot of yellow peach (Amygdalus persica) in China

Yellow peach (Amygdalus persica) is an economically important fruit crop in China, and is a specialty of Fengxian district in Shanghai. During July to September 2017, losses of at least 10% associated with fruit rot of yellow peaches (cv. …

Diversity and Characterization of Endophytic Fungi Isolated From the Tropical Mangrove Species, Rhizophora mucronata, and Identification of Potential Antagonists Against the Soil-Borne Fungus, Fusarium solani.

Rhizophora mucronata is an important ecosystem entity of the Malaysian mangrove forest. Since the species grows in a harsh environment, any organism that is isolated from this species would be of huge interest due to its potential in having novel bioactive compounds. In the present work, we isolated, identified and characterized, a total of 78 fungal isolates harboring inside the leaf tissues of R. mucronata. Molecular identification using the nuclear ribosomal DNA internal transcribe spacer (ITS) sequences returned with high similarity matches to known sequences in the GenBank. Maximum likelihood analysis revealed the phylogenetic relationship of all isolates from this study. Most of the dominating fungal endophytes were from the genera Pestalotiopsis, followed by Alternaria and Cladosporium. Six isolates representing the genera Alternaria, Fusarium, Nigrospora, Pestalotiopsis, Phoma, and Xylaria, were further screened for their antagonism activities. Dual culture test assay revealed their inhibition percentages against the phytopathogenic fungus Fusarium solani between 45–66%, and 0.8–23% when using non-volatile test assay. Of the six isolates, only Fusarium lateritium and Xylaria sp. showed antibacterial activities against the pathogenic bacteria, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus, with the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) ranging from 0.5 to 2 mg/mL. The DPPH radical scavenging assay recorded a high level of antioxidant activity in Xylaria sp., 3-fold above that of F. lateritium. We demonstrate for the first time, two members belonging to the endophytic fungal community in the tropical mangrove species that have potential use as antagonists and antibacterial agents for future biotechnological applications.

First report of canker disease in Dalbergia tonkinensis caused by Fusarium lateritium and Fusarium decemcellulare

Dalbergia tonkinensis is a precious tree species of high economic value. Due to an increase in planting activities in the past few years in Vietnam, the provision of healthy planting stock is in high demand. Canker disease, which can affect the growth and quality of the plant, has been found to occur on scattered old trees and in D. tonkinensis plantations. This study aimed to identify symptoms, and to characterize the morphology, taxonomy and pathogenicity of Fusarium pathogens causing canker disease in D. tonkinensis in North Vietnam. Typical symptoms of disease caused by Fusarium spp. in D. tonkinensis included stem and branch canker, and darkening of the wood, leading to the canopy on diseased stems becoming yellow, followed by wilting, drying and premature leaf fall. From ITS1 and ITS4 sequence analysis, the Fusarium isolates causing canker disease in D. tonkinensis in Vietnam belong to Fusarium decemcellulare and Fusarium lateritium. These species were pathogenic in inoculation trials using detached branches and seedlings. The pathogenicity of isolates ranged from moderate to highly virulent. In conclusion, canker disease caused by F. lateritium and F. decemcellulare is quite common in North Vietnam, and options for disease management need to be developed in further domestication of this threatened species.

Identifikasi Mikroorganisme Tanah dan Serangga yang Berasosiasi dengan Pohon Gaharu

<p>Papua is considered as one of the centre of gaharu productions in Indonesia, because 58% of its natural diversification exist in this region. �The soil microorganism and insect have been known as vector for the penetration of �Fusarium lateritium which cause the gaharu effect.� This research was aimed to identify �both the soil microorganism and insect that associated with gaharu plant.�The result of the study revealed that (1) The soil microorganism that live in gaharu habitate were Trichoderma viridae, Fusarium spp, Corynebacterium sp., Criconemoides sp., and �Pratylenchus sp., and (2) The insect that live in gaharu habitate were Anomala pallida, Hypomyces inflatus, Arachnoidea, Dolichoderes bituberculatus, Oecophylla smaragdina, Dysdercus cingulatus, Teleogryllus mitratus, and dragon Flies.</p>

Isolation, Identification and Biomanagement of Root Rot of Black Cumin (Nigella sativa) Using Selected Bacterial Antagonists

The study deal with potentiality of some bioagents for controlling the root rot of black cumin under greenhouse conditions, caused by Fusarium spp. Eight fungal isolates were obtained from diseased of back cumin plants collected from Assiut Governorate. These isolates were belonged to the genus Fusarium spp. They were identified as, four isolates of F. comptoceras, three isolates of F. solani and one isolate Fusarium lateritium. Pathogenicity tests indicated that all tested fungal isolates were able to infect black cumin plants causing symptoms of root rot resulted in dwarfism and death before the capsules mature. They varied in their pathogenicity, Fusarium comptoceras No.1 gave the highest percentage of disease severity and percentage of infection on black cumin plants (53 and 50% respectively), while isolates F. comptoceras Nos. 3 and F. solani No. 6 gave the lowest percentage of infection (15 and 17% respectively) the rest of isolates showed moderate of percentage of infection. Antagonistic capability of 15 isolates (PGPR) was tested in vitro against growth of three isolates of Fusarium spp. the causal pathogen of root rot of black cumin. Seeds black cumin plant treated with all bioagents as a suspension significantly increased the root dry weigh and foliar dry weigh compared to infected control. In conclusion, our study confirmed that used of bioagents may be applied as future ecofriendly alternatives to synthetic fungicides for controlling the disease of black cumin.

Two New Pyripyropenes from the Marine Fungus Fusarium lateritium 2016F18-1.

Two new pyripyropenes, 13-dehydroxy-1,11-deacetylpyripyropene A (1) and 1-deacetylpyripyropene A (2), together with six known compounds, were isolated from a marine fungus Fusarium lateritium 2016F18-1 which was associated with the sponge Phyllospongia foliascens. Their structures were established mainly based on NMR and MS data. Their cytotoxic activities against human cancer cells CNE1, CNE2, HONE1, SUNE1, GLC82, and HL7702 were evaluated.

Metabolic activity of Aspergillus niger and Fusarium lateritium induced by ethoxylated oleyl cetyl alcohol and their bioremediation and biotechnological potential

The effect of ethoxylated oleyl cetyl alcohol (Henkel, Serbia) on the growth and metabolic activity of Aspergillus niger and Fusarium lateritium was in the focus of this paper. The fungi were isolated from wastewater of Lepenica River (Kragujevac, Serbia) at a place where municipal wastewater discharged into the river. The fungi were grown in Czapek-Dox liquid nutrient medium without and with addition of 0.5% pollutant. The physico-chemical and biochemical changes of pH, total biomass dry weight, quantity of free and total organic acids, proteolytic activity and quality of carbohydrates were evaluated from 4-th to 19-th day of fungal growth. The capacity of fungi to decrease concentration of pollutant in medium was determined by cobalt thiocyanate method. The pollutant caused an inhibitory effect on biomass dry weight of A. niger and F. lateritium for 8.50 and 30.61%, respectively. Among tested fungi, A. niger had the better biodegradation capacity (83%) than F. lateritium (65%). Alkaline protease activity of A. niger enhanced in the presence of pollutant for 7.6% whereas the enzyme of F. lateritium retained about 62.2% activity. Overall, the obtained results indicate the potential application of tested fungi in wastewater treatment, detergent industry and biotechnology.

Purification, biochemical characterization, and antimicrobial activity of a new lipid transfer protein from Coffea canephora seeds.

Coffee, an agronomical crop of great economic importance, is also among the most commonly traded commodities in worldwide markets. Antimicrobial peptides, which play a role in plant defense, have been identified and isolated particularly from seeds. We isolated and immunolocalized Cc-LTP2, a new lipid transfer protein (LTP) from Coffea canephora seeds. We report its antimicrobial activity against various phytopathogenic fungi of economic importance, and against the bacterium Xanthomonas euvesicatoria. Peptides from C. canephora seeds were initially extracted using acid buffer and subjected to ion-exchange and reverse-phase chromatographies. A purified peptide of approximately 9 kDa, which we named Cc-LTP2, was then subjected to amino acid sequencing. The analyses showed that it was similar to LTPs isolated from various plants. The tissue and subcellular localization of C. canephora LTPs indicated that they were located in cell walls and intracellular palisade parenchyma, mainly in large vacuoles. The results of immunohistochemistry and histochemistry superposed from C. canephora seed tissues showed that LTPs and lipid bodies are present in organelles, supporting the hypothesis that LTPs from seeds are involved in lipid mobilization during germination. Cc-LTP2 did inhibit the development of the phytopathogenic fungi Colletotrichum lindemuthianum, Colletotrichum gloeosporioides, Fusarium solani, Fusarium lateritium, and Colletotrichum sp, but did inhibit X. euvesicatoria. Cc-LTP2 also increased membrane permeability and induced endogenous production of reactive oxygen species in all the fungi tested.

Biomass yield, nutritive value and accumulation of minerals in Trifolium subterraneum L. as affected by fungal endophytes

Trifolium subterraneum is a legume species which is valuable for feeding livestock and frequently used as a forage crop in countries with temperate or Mediterranean-like climates. The aim of the present work was to evaluate the effect of six leaf fungal endophytes on biomass production, nutritive value and mineral status of T. subterraneum forage. Plants were inoculated with each of seven treatments (six endophytes + control) at two different growth stages. After inoculation, two experiments (under greenhouse and field conditions) were established. Endophytes affected biomass yield, nutritive value and mineral status of T. subterraneum forage, but effects varied between experiments and depended on fungal species. E202 (Chaetosphaeronema sp.) increased forage productivity by around 80 % in the field. Fusarium lateritium and E244 (Pleosporales) reduced Al concentration, and Epicoccum nigrum reduced Pb of the forage in the greenhouse experiment. An increase in essential nutrients, such as Zn, was mainly produced by Stemphylium globuliferum. This study demonstrated that inoculation with endophytes can increase forage productivity and help reduce potential nutrient deficiencies and/or potential mineral toxicity in T. subterraneum.

English

Eretmochelys imbricata Linneus 1766 was the subject of trade due to egg collection and consumption of the flesh of the females, being the fishery one of the main impacts towards the coastal area. The pathogens are also worrying factors of mortality of sea turtles especially those caused by fungi; these can cause the death of embryos and cutaneous mycoses. This study aimed to investigate the mycoflora isolated from soil, eggshells and stillbirths from E. imbricata in three beaches of Ipojuca (Brazil). We recorded data on the reproductive biology of the species after incubation of nests. Soil samples and fragments of eggshells were collected at the end of the nesting season for fungi identification. A total of eight species of fungi were identified by their morphological characteristics: Aspergillus terreus, Aspergillus niger, Aspergillus flavus, Cladosporium cladosporioides, Nigrospora grisea, Fusarium solani, Fusarium lateritium and F. oxysporum in the soil samples, eggshells and stillbirths. Fusarium was recorded in other studies interfering with the development of turtles embryos. The data from this study will provide information to support the management and conservation of sea turtles. Key words: Eggshell, fungi, Fusarium, Hawksbill, testudines.