Penaeus Monodon Research Articles

Elucidation of synbiotic diet comprising of Lactobacillus plantarum L20 and Sargassum polycystum on gastrointestinal microbiota, tissue structures and AHPND associated dysbiosis susceptibility in black tiger shrimp (Penaeus monodon)

This study investigates the effect of a synbiotic combination comprising of Lactobacillus plantarum L20 and Sargassum polycystum on gastrointestinal microbiota, tissue structures and AHPND infection in black tiger shrimp, P. monodon. The shrimp were randomly distributed into synbiotic (feed that was incorporated with 108 CFU/g of L. plantarum L20 and 2% S. polycystum) and control group (feed that was incorporated with PBS). After 35 days of feeding trial, all groups were subjected to an AHPND-causing V. parahaemolyticus S2–4 immersion challenge at 6 × 105 CFU/mL. The shrimp gastrointestinal tract from both groups were collected from the feeding trial and challenge test and subjected to high throughput 16S rRNA amplicon sequencing and histopathological analysis. Following 35th day of feeding trial, there were growth enhancement in shrimp, but no significant differences (p > 0.05) in alpha and beta diversities in gastrointestinal microbiota for both groups. However, synbiotic group exhibited a differential abundance of beneficial bacteria, including Lactobacillaceae and Lactiplantibacillus, which were not observed in the control group. Following 60 h post-challenge, there was improvement in shrimp survival in synbiotic group, with slightly significant differences (p < 0.05) were observed in alpha diversity and but no significant differences (p > 0.05) were observed in beta diversity. Synbiotic group demonstrated negative correlation between the gastrointestinal microbiota with Vibrionaceae, enriched potential metabolic pathway of energy generation and microbiota proliferation, and protected gastrointestinal tract structure during the study. The findings suggest that synbiotic diet comprising of L. plantarum L20 and S. polycystum improve growth performance, disease resistance and gastrointestinal structure protection through enhancement of few beneficial bacteria group abundance, despite insignificantly changes of gastrointestinal microbiota with control diet in the shrimp.

Metabolic activity of gut microbial enrichment cultures from different marine species and their transformation abilities to plastic additives

The role of the gut microbiota in host physiology has been previously elucidated for some marine organisms, but little information is available on their metabolic activity involved in transformation of environmental pollutants. This study assessed the metabolic profiles of the gut microbial cultures from grouper (Epinephelus coioides), green mussel (Perna viridis) and giant tiger prawn (Penaeus monodon) and investigated their transformation mechanisms to typical plastic additives. Community-level physiological profiling analysis confirmed the utilization profiles of the microbial cultures including carbon sources of carbohydrates, amines, carboxylic acids, phenolic compounds, polymers and amino acids, and the plastic additives of organophosphate flame retardants, tetrabromobisphenol A derivates and bisphenols. Using in vitro incubation, triphenyl phosphate (TPHP) was found to be rapidly metabolized into diphenyl phosphate by the gut microbiota as a representative ester-containing plastic additive, whereas the transformation of BPA (a representative phenol) was relatively slower. Interestingly, all three kinds of microbial cultures efficiently transformed the hepatic metabolite of BPA (BPA-G) back to BPA, thereby increasing its bioavailability in the body. The specific enzyme analysis confirmed the ability of the gut microbiota to perform the metabolic reactions. The results of 16S rRNA sequencing and network analysis revealed that the genera Escherichia-Shigella, Citrobacter, and Anaerospora were functional microbes, and their collaboration with fermentative microbes played pivotal roles in the transformation of the plastic additives. The structure-specific transformations by the gut microbiota and their distinct bioavailability deserve more attention in the future.

Growth, Enzyme Activity, Score, and Essential Amino Acid Index of the Asian Tiger Shrimp (Penaeus monodon Fab. 1798) Supplemented with Multi Enzyme

Growth, Enzyme Activity, Score, and Essential Amino Acid Index of the Asian Tiger Shrimp (Penaeus monodon Fab. 1798) Supplemented with Multi Enzyme

Heavy Metal Residues and Bacterial Pathogens of Food Safety Significance in Edible Crustacean from Crude-Oil-Impacted Niger Delta, Nigeria

ABSTRACT The study investigated heavy metal residues and bacterial pathogens in blue crab (Callinectes amnicola), crayfish (Nematopaelemon hastatus), giant tiger prawn (Penaeus monodon), and Indian prawn (Fenneropenaeus indicus) from crude oil impacted Niger Delta waters. Standard methods of analysis were applied in all determinations. Results indicate that chromium, lead, and mercury were within regulatory acceptable levels. Total viable count (TVC) was below threshold, while Vibrio cholerae, Vibrio parahaemolyticus, and Salmonella typhimurium were isolated from all crustacean species. The distribution pattern of pathogens showed blue crab accumulated the highest population of pathogens. Consumption of crustaceans from the location exemplifies conflict of nutrient benefits and risks.

Full nutritional spectrum and hazard risk assessment of high commodity value aquaculture species in Penang, Malaysia

Aquaculture is a rapidly growing food production sector that aims to alleviate global hunger, malnutrition, food security and nutrient deprivation. This study aimed to update the full spectrum of nutritional composition using aquaculture species. High commodity value aquaculture species in Penang, Malaysia have been shortlisted, followed by nutritional quantification and health risk estimation. Nutritional compositional analysis involved proximate analysis, and amino acid, fatty acid, vitamin, mineral and heavy metal quantification. Health risk assessment was conducted to determine whether the benefits were greater than the risks in the long term. Ten (10) aquaculture species, namely golden pomfret, golden snapper, seabass, bigeye trevally, red snapper, grouper, African catfish, blood cockles, white-leg shrimp and tiger prawn, have been studied extensively. The results showed that all species contained adequate protein and lipid contents, ranging from 13.6 % to 26.9 % and 0.4–15.8 %, respectively. The essential amino acid (EAA) to total amino acid (TAA) ratio of the culture species was comparable to the FAO reference values (50 %), with leucine and lysine demonstrating the most important EAAs. Fatty acid profiling revealed that both seabass and grouper were rich in polyunsaturated fatty acids, particularly docosahexaenoic acid. Golden pomfret and red snapper contained the greatest amounts of riboflavin and pyridoxine. The atherogenic and thrombogenic indices were within the permissible ratio (<1), except for those of African catfish. Notably, the arsenic (As) content in blood cockles exceeded the permissible criterion values. Hazard index (HI) assessment indicated that prolonged consumption of blood cockles, white-leg shrimp, tiger prawn, bigeye trevally and golden snapper demonstrated potent non-carcinogenic risk for both adults and children. This study provides an initial platform for informing consumers, stakeholders and policy makers about aquaculture nutritional benefits, consumption safety concern and health indicators.

Detection of pathogenic bacteria in retailed shrimp from Bangladesh

AbstractThe presence of pathogenic bacteria is a problem that might be present in farmed shrimp due to exposure in the environment or post‐harvest handling. Retail farmed shrimp in Bangladesh (Penaeus monodon and Macrobrachium rosenbergii) were tested for common pathogenic bacteria namely Salmonella, L. monocytogenes, Vibrio spp., and E. coli. None of these bacteria were found and instead Enterobacter cloacae, Escherichia fergusonii, Proteus penneri, Klebsiella aerogenes, Enterococcus faecalis, Serratia marcescens, Citrobacter freundii, and Aeromonas dhakensis were detected. Pathogenic bacteria found in Bangladeshi shrimp may be due to the farm environment, poor handling during harvest or post‐harvest, or unhygienic market conditions. The results indicate that retail shrimp from Bangladesh have food safety concerns. Proper laws and policies need to be enforced and implemented to ensure food safety related to fish and shrimp.

Ago4-piRNA complex is a key component of genomic immune system against transposon expression in Penaeus monodon

Argonaute proteins are key constituents of small RNA-guided regulatory pathways. In crustaceans, members of the AGO subfamily of Argonaute proteins that play vital roles in immune defense are well studied, while proteins of the PIWI subfamily are less established. PmAgo4 of the black tiger shrimp, Penaeus monodon, though phylogenetically clustered with the AGO subfamily, has distinctive roles of the PIWI subfamily in safeguarding the genome from transposon invasion and controlling germ cell development. This study explored a molecular mechanism by which PmAgo4 regulates transposon expression in the shrimp germline. PmAgo4-associated small RNAs were co-immunoprecipitated from shrimp testis lysate using a PmAgo4-specific polyclonal antibody. RNA-seq revealed a majority of 26–27 nt long small RNAs in the PmAgo4-IP fraction suggesting that PmAgo4 is predominantly associated with piRNAs. Mapping of these piRNAs on nucleotide sequences of two gypsy and a mariner-like transposons of P. monodon suggested that most piRNAs were originated from the antisense strand of transposons. Suppression of PmAgo4 expression by a specific dsRNA elevated the expression levels of the three transposons while decreasing the levels of transposon-related piRNAs. Taken together, these results imply that PmAgo4 exerts its suppressive function on transposons by controlling the biogenesis of transposon-related piRNAs and thus, provides a defense mechanism against transposon invasion in shrimp germline cells.

Transcriptomic Insights and the Development of Microsatellite Markers to Assess Genetic Diversity in the Broodstock Management of Litopenaeus stylirostris.

The Pacific blue shrimp (Litopenaeus stylirostris) is a premium product in the international seafood market. However, intensified farming has increased disease incidence and reduced genetic diversity. In this study, we developed a transcriptome database for L. stylirostris and mined microsatellite markers to analyze their genetic diversity. Using the Illumina HiSeq 4000 platform, we identified 53,263 unigenes from muscle, hepatopancreas, the intestine, and lymphoid tissues. Microsatellite analysis identified 36,415 markers from 18,657 unigenes, predominantly dinucleotide repeats. Functional annotation highlighted key disease resistance pathways and enriched categories. The screening and PCR testing of 42 transcriptome-based and 58 literature-based markers identified 40 with successful amplification. The genotyping of 200 broodstock samples revealed that Na, Ho, He, PIC, and FIS values were 3, 0.54 ± 0.05, 0.43 ± 0.09, 0.41 ± 0.22, and 0.17 ± 0.27, respectively, indicating moderate genetic variability and significant inbreeding. Four universal microsatellite markers (CL1472.Contig13, CL517.Contig2, Unigene5692, and Unigene7147) were identified for precise diversity analysis in Pacific blue, Pacific white (Litopenaeus vannamei), and black tiger shrimps (Penaeus monodon). The transcriptome database supports the development of markers and functional gene analysis for selective breeding programs. Our findings underscore the need for an appropriate genetic management system to mitigate inbreeding depression, reduce disease susceptibility, and preserve genetic diversity in farmed shrimp populations.

Effects of stocking density and salinity on the flavour performance and muscle microstructure of black tiger shrimp (Penaeus monodon) post larvae

Effects of stocking density and salinity on the flavour performance and muscle microstructure of black tiger shrimp (Penaeus monodon) post larvae

Study on the mechanism of miR-7562 regulating ATG5 and ATG12 genes in Penaeus monodon under Vibrio harveyi infection

MicroRNAs (miRNAs) play a fundamental role in the post-transcriptional regulation of genes and are pivotal in modulating immune responses in marine species, particularly during pathogen assaults. This study focused on the function of miR-7562 and its regulatory effects on autophagy against Vibrio harveyi infection in the black tiger shrimp (Penaeus monodon), an economically important aquatic species. We successfully cloned and characterized two essential autophagy-related genes (ATGs) from P. monodon, PmATG5 and PmATG12, and then identified the miRNAs potentially involved in co-regulating these genes, which were notably miR-7562, miR-8485, and miR-278. Subsequent bacterial challenge experiments and dual-luciferase reporter assays identified miR-7562 as the principal regulator of both genes, particularly by targeting the 3′UTR of each gene. By manipulating the in vivo levels of miR-7562 using mimics and antagomirs, we found significant differences in the expression of PmATG5 and PmATG12, which corresponded to alterations in autophagic activity. Notably, miR-7562 overexpression resulted in the downregulation of PmATG5 and PmATG12, leading to a subdued autophagic response. Conversely, miR-7562 knockdown elevated the expression levels of these genes, thereby enhancing autophagic activity. Our findings further revealed that during V. harveyi infection, miR-7562 continued to influence the autophagic pathway by specifically targeting the ATG5-ATG12 complex. This research not only sheds light on the miRNA-dependent mechanisms governing autophagic immunity in shrimp but also proposes miR-7562 as a promising target for therapeutic strategies intended to strengthen disease resistance within the crustacean aquaculture industry.

Utilization of Milkfish Bones and Heads from CV. Maharani Dwi Bayu Kalanganyar Sidoarjo for Making Spicy Fish Sauce

Kalanganyar is a village whose 2/3 area consists of ponds and producers of milkfish, tiger prawns, shrimp paste, processed milkfish and crackers. Most of the milkfish from Kalanganyar are sold fresh, but some are processed into various processed products, including smoked milkfish, shredded milkfish, crackers, soft spiny milkfish and crispy milkfish. One of the producers of crispy milkfish is CV. Maharani Dwi Bayu. Milkfish that is processed into crispy milkfish will leave behind waste in the form of heads, tails and milkfish bones/spines. So far, this waste has not been utilized optimally. To overcome this problem, the heads and bones of milkfish are used as raw materials for making spicy fish sauce. The resulting soy sauce can be used as a complement to smoked milkfish or presto milkfish as a substitute for chili sauce. Training has been carried out on processing fish sauce at CV. MAHARANI DWI BAYU, Jalan Raya Kalanganyar Wetan, Sedati District, Sidoarjo Regency. Apart from holding training on processing fish sauce from milkfish bones and heads, supporting equipment for soy sauce processing was also handed over, namely pressure cookers, vacuum filters, steamers and hot guns. The people of Kalanganyar are very happy and enthusiastic about taking part in training to gain new knowledge and new innovative products.

Cytokine-like-Vago-mediated antiviral response in Penaeus monodon via IKK-NF-κB signaling pathway

Interferon (IFN) system is the primary mechanism of innate antiviral defense in immune response. To date, limited studies of IFN system were conducted in crustaceans. Previous report in Penaeus monodon demonstrated the interconnection of cytokine-like molecule Vago and inhibitor of kappa B kinase-nuclear factor κB (IKK-NF-κB) cascade against white spot syndrome virus (WSSV). This study further identified five different PmVago isoforms. Upon immune stimulation, PmVagos expressed against shrimp pathogens. PmVago1, PmVago4, and PmVago5 highly responded to WSSV, whereas, PmVago1 and PmVago4 RNAi exhibited a rapid mortality with elevated WSSV replication. Suppression of PmVago1 and PmVago4 negatively affected proPO system, genes in signal transduction, and AMPs. WSSV infection additionally induced PmVaog4 granule accumulation and cellular translocation to the area of cell membrane. More importantly, PmVago1 and PmVago4 promoters were stimulated by PmIKK overexpression; meanwhile, they further activated Dorsal and Relish promoter activities. These results suggested the possible roles of the cytokine-like PmVago via IKK-NF-κB cascade against WSSV infection.

Development of an artificial propagation and breeding method for the beehorn snail (Clithon diadema)

Beehorn snail (Clithon diadema), known for its vibrant colors, is threatened with extinction due to excessive field collection. Therefore, this study investigated the effects of different diets and salinity (0‰–35‰) on the gonadal development, capsule productivity, embryonic development, and larval survival and growth of C. diadema. Adult snails (shell length: 9.09 ± 0.87 mm) were fed diatoms, including pennate marine diatom (Nitzschia grossestriata), boat-shaped diatom algae (Navicula sp.) and Cylindrotheca sp., along with giant tiger prawn (Penaeus monodon) commercial feed, sea lettuce (Ulva lactuca), or Cladophora fascicularis, while snail larvae (shell length: 154.85 ± 6.03 μm) were fed Isochrysis galbana or Chaetoceros muelleri. The diatom group outperformed commercial feed and U. lactuca groups. The highest capsule productivity, yielding 79–82 capsules in 4 weeks, occurred at 15‰-20‰ salinity for diatom-fed adults. Embryos thrived at 0‰ but didn't hatch; hatching required salinities above 0‰, highlighting saline necessity. In addition, acute salinity changes (0‰ to 30‰) can deform embryo. Embryonic development at 15‰–20‰ salinity lasted 13.56–14.33 days with 100% larval hatching. However, larvae hatched at >20‰ salinity exhibit improved the percentage of survival without feeding (longer median lethal times). I. galbana-fed larvae survived 68 days with a 75% rate, outlasting C. muelleri-fed larvae which perished within 28 days. I. galbana-fed larvae, raised for 68 days at 25‰ salinity, showed 100% survival, 44.44% metamorphosis, and a 3.1 times growth increase. Metamorphosis rates were highest at 25‰ salinity with I. galbana, exceeding the 20‰ group by 3.2 times. Mucus of C. diadema boosts larval metamorphosis to 50.00%, 9 times higher than without inducers. Based on previous results, we developed an artificial propagation and breeding method for C. diadema. Artificially propagating C. diadema in large quantities could reduce the need for wild harvesting, thereby mitigating negative ecological impacts from excessive collection.

Identification of microRNAs in black tiger shrimp (Penaeus monodon) under acute low-salinity stress

Salinity is a common abiotic stress in the culture of penaeid shrimp. Through post-transcriptional regulation of gene transcripts, microRNAs (miRNAs) play an important role in the adaptation to a stressful environment. However, the involvement of miRNAs in the salinity stress response of shrimp remains unclear. In the present study, the sequence and expression profile of miRNAs in the hepatopancreas of low-salinity-treated Penaeus monodon were obtained by the high-throughput sequencing technique. A total of 679 miRNAs were identified, including 167 miRNAs that were significantly differentially expressed after low-salinity exposure (p &amp;lt; 0.05). Remarkably, most of these miRNAs were downregulated, suggesting that a series of genes were activated to participate in stress response. In addition, 43 miRNAs differentially expressed at all treatment were selected as putative key modulators. Enrichment analysis of genes targeted by these miRNAs indicated that a network that consists of the nervous system, the immune system, and the endocrine system played a crucial role in maintaining the homeostasis of P. monodon under low-salinity stress. These findings may help contribute to a better understanding of the mechanism that regulates salinity tolerance in shrimp and provide valuable genetic information for subsequent studies.

Solid-state fermentation converts rice bran into a high-protein feed ingredient for Penaeus monodon

Fermented rice bran (FRB) was evaluated as an alternative protein source to soybean meal (SM) in practical diets for juvenile black tiger shrimp, Penaeus monodon. This feed ingredient was tested in a feeding trial to replace soybean meal in P. monodon diets at 0% (T0), 12.5% (T12.5), 25% (T25), 37.5% (T37.5), and 50% (T50). Five iso-nitrogenous and iso-caloric experimental diets containing 44% crude protein were fed to groups of juvenile shrimp (0.47 ± 0.002 g) randomly assigned to twenty 60-l rectangular tanks equipped with a recirculating seawater system. Each dietary treatment was run in 4 replicates, and the feeding trial lasted 50 days. Results show significant improvement in weight gain, specific growth rate, and protein efficiency ratio in treatment T12.5 and T25. Treatments with higher levels of SM replacement with FRB exhibited similar growth indices to those of the control group. Polynomial regression analysis indicates that the optimum replacement of soybean meal with FRB for optimum growth is 21.08%. The apparent dry matter and protein digestibility coefficients of FRB are 83.05 ± 0.02% and 87.20 ± 0.30%, respectively. There were no significant differences in the whole-body composition (dry matter, protein, lipid, ash) among treatments of shrimp fed with FRB replacement. The data suggest that FRB replacement of dietary soybean meal is feasible at 50% without affecting the growth performance but may promote growth at 21.08% replacement of P. monodon.

Analisis Usaha dan Pemasaran Pembenihan Udang Windu (Penaeus monodon) di HSRT Windu Tirta Samudra Desa Tanjung Limau Kecamatan Muara Badak

Tiger shrimp farming is an aquaculture business associated with the rearing of shrimp from egg hatching until they are ready to be harvested for consumption. The amount of tiger shrimp production is highly dependent on the tiger shrimp hatchery process. This study was conducted with the aim to 1) Calculate the income of tiger shrimp hatchery business, 2) Analyze the feasibility of tiger shrimp hatchery business based on the criteria of Revenue Cost Ratio (RCR), Break Even Point (BEP), Payback Period, Return On Investman (ROI), and 3) Describe marketing channels, marketing margins, and farmer share in shrimp hatchery business. The sample method used was purposive sampling and snowball sampling. The results showed that tiger shrimp hatcheries in HSRT Windu Tirta Samudra earned an income of Rp.43,313,992/month. Based on the criteria of RCR, BEP, ROI and Payback Period (PP) tiger shrimp hatchery business is feasible to continue and run. Marketing of tiger shrimp seed forms level 0 and level 1 marketing channels, with a margin of Rp.2/head on level 1 marketing channels, and the farmer's share obtained is efficient because it is above 60%.

Antimicrobial mechanism of linalool against Vibrio parahaemolyticus and its application in black tiger shrimp (Penaeus monodon)

Vibrio parahaemolyticus (V. parahaemolyticus) is a seafood-derived pathogen that poses a serious threat to seafood and human health. Biofilm production is a necessary process for V. parahaemolyticus to colonize the host and release virulence factors. Therefore, preventing biofilm formation or eliminating biofilm cells is an effective strategy to combat V. parahaemolyticus infection. Linalool is a food additive of natural origin with excellent antibacterial activity. In this study, we investigated the antimicrobial and anti-biofilm effects of linalool against V. parahaemolyticus and elucidated its mechanism through metabolomics analysis. The results showed that the minimum inhibitory concentration (MIC) of linalool against V. parahaemolyticus is 1.7 mg/mL. The treatment with linalool disrupted the integrity of the cell membrane, resulting in the leakage of nucleic acids and proteins, impaired motility, and reduced release of extracellular polysaccharide (EPS). The metabolomics results revealed that linalool perturbed the metabolic pathways of amino acids and nucleic acids in V. parahaemolyticus, thereby impeding biofilm production. Moreover, linalool significantly inactivated V. parahaemolyticus in black tiger shrimp. These findings have practical implications for the utilization of linalool in controlling bacterial contamination in seafood and enhancing food safety.

The tracement of origins of aquatic products by characterization of their gut or gill microbiome via MALDI-TOF MS analysis

Tracement of the origin of seafoods and aquatic products is an important issue for avoiding the falsification of production regions of aquatic products. Herein, by analyzing black tiger shrimp (Penaeusmonodon) as a model of aquatic products, we demonstrated a method to trace the origin of aquatic products by analyzing the gut microbiome of black tiger shrimp (Penaeusmonodon) collected from three commercial farms in China, via matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. Multivariate analysis was executed on the obtained mass spectrometric datasets of bacteria extracted and collected from shrimp samples with different production regions. The gut microorganisms of black tiger shrimps can be identified by scoring the gut microbiota of black tiger shrimps collected from three producing areas. The black tiger shrimp from three production regions were found with distinguished characteristics of bacterial prevalence in their digestive gland microbiota. The dominant bacteria genus in digestive gland microbiota from black tiger shrimp samples from the three production regions are completely different. Single-blind shrimp samples with unknown production region was successfully screened by comparing the detected MS data in different ways. This strategy can trace the production region of the analyzed shrimp samples in a quick and efficient mode. The application of the present protocol was further demonstrated by analyzing the biometric species from gill tissue of Chinese mitten crab (Eriocheir sinensis) samples collected from crab-producing areas in Jiangsu Province (China), that is, Taihu Lake, Gaoyou Lake, and Hongze Lake for tracing their production area. It is expected to extend the presently developed method to provide clues for the origin traceability of other aquatic products.

Growth And Food Habits Of Red Snapper (Lutjanus Malabaricus) Catched In The Waters Of Central Mamuju, Makassar Strait

Red snapper is a basic fish that has economic value with intensive use and high market demand, so it is thought to have undergone biological changes. In this regard, the research aims to analyze the size distribution, growth and food habits of red snapper caught in Mamuju waters. This research is classified as a survey type with data sources from fishermen's catches. The results showed that the average size distribution of red snapper from Kambunong was ± 36.93 cm for males and ± 44.65 cm for females, Babana ± 29.81 cm for males and ± 41.14 cm for females, Pangale ± 31.45 cm for males and ± 33.75 cm female. The growth pattern of samples from Pangale and Kambunong was negative allometric, while Babana had a positive allometric pattern. The main types of food are white shrimp, tiger prawns, lencam fish, crab and lobster while cuttlefish is a complementary food. To catch red snapper, both males and females should use red snapper as their main food

Effectiveness And Composition Of Anco Net (Lift Net) In Bungko Lor Waters, Cirebon District, West Java

Cirebon Regency is topographically located at an altitude of 0-130 km above sea level and is located along the north coast of Java. Geographically and demographically, Cirebon Regency is very suitable for the development of capture fisheries potential, seen from the livelihoods of the population who work as fishermen. The potential of fishery resources in cirebon district revealed a situation is very possible to be developed in order to economic development in cirebon.The net anco ( elevator net ) is one of kinds of tools catch the fish environment friendly. very simpleOne of those potential fishing in bungko lor need to further review, relating to the operation of an instrument. The community fishermen bungko lor cirebon district is still hampered on increased productivity in an effort do police operations. The purpose of this study is to find the effectiveness and composition of the fish anco (elevator net) that is operated on day and night of the results of the water at around 6 catch bungko lor .A method of descriptive of the receipt of the data is survey and direct observation in the field as well as in data collection by staying tightly focused in a case .The sampling method of collected data based on fishermen time operating in waters bungko lor later in analysis using chi-square t test and test it. Catch the elevator anco ( net ) during research consisting of 4 types of shrimp jerbung ( penaeus merguiensis deman ), shrimp windu ( penaeus monodon ), fish mullet ( moolgarda seheli ), and bloso ( glossogobius giuris ).Shrimp jerbung catch is the most during the research 37,1 kg of ( 82 % ), shrimp windu 4,8 kg of ( 9 % ), fish mullet 3,9 kg of 7 % and fish bloso 0,9 kg of 2 %.