Vespa Mandarinia Research Articles

Detection and description of four Vespa mandarinia (Hymenoptera, Vespidae) nests in western North America

Vespa mandarinia Smith 1852 is a semi-specialized predator of other social Hymenoptera and one of the two largest species of Vespa. Several individuals of this predatory wasp were detected in Canada and the United States in 2019, including an entire nest that was located and destroyed on Vancouver Island, British Columbia. The Washington State Department of Agriculture and the United States Department of Agriculture’s Animal and Plant Health Inspection Service have collaborated to survey Washington State for V. mandarinia since 2020, using traps staffed by agency personnel, collaborators from local governments and nongovernmental organizations, and the general public. Trap data and public reports were used to select sites for live-trapping or net surveys, and live hornets captured in these efforts were subsequently collected and fitted with radio tags to locate nests. The survey ultimately led to the discovery of a V. mandarinia nest in October 2020, and three nests in August and September 2021. All of the nests were located within in red alder trees (Alnus rubra), with one just above the ground in a standing dead tree, and the other three in cavities ~2 to 5 meters above the ground in living trees. The number of combs in each nest varied between four and ten, cells between 418 and 1,329, and total hornets per nest between 449 and 1,474 (including immature and mature stages). Together, the four nests indicate an incipient population of V. mandarinia in the Cascadia region, and ongoing action by local, state, provincial, and federal governments, and residents of both countries is required to avoid the establishment of this exotic species in the region.

Insights into the prey of Vespa mandarinia (Hymenoptera: Vespidae) in Washington state, obtained from metabarcoding of larval feces.

The northern giant hornet, Vespa mandarinia (Hymenoptera: Vespidae), was detected for the first time in North America in 2019. Four nests have since been located and removed in northwestern Washington State as part of an extensive survey and eradication program. This recent introduction into North America has prompted new research on the biology and ecology of V. mandarinia to help inform management strategies. In its native range, V. mandarinia is known to prey on a variety of insects including the economically important honey bee species Apis cerana and Apis mellifera. Although A. cerana has developed defense mechanisms against attack by V. mandarinia, A. mellifera have no such defenses and an entire hive can be quickly destroyed by only a few hornets. In North America the hornet has been observed foraging on paper wasps (Polistes dominula) and honey bees, but little else is known about prey use in its novel range. To address this knowledge gap, we employed a DNA metabarcoding approach to characterize species detected in larval feces collected from 3 of the 4 Washington V. mandarinia nests found to date. Sequences were recovered for 56 species across fourteen orders, of which 36 species were likely prey items and 20 were suspected inquilines. The most frequently detected species were other social Hymenoptera, with Dolichovespula maculata, P. dominula, and A. mellifera present in most samples. All of the species detected, except for A. mellifera, represent new prey records for V. mandarinia, with eight families of insects newly associated with giant hornets. These results suggest that V. mandarinia in Washington preys on an assortment of insects similar to those documented in its native range, and that this new invader has readily incorporated novel species into its foraging and diet.

Temporal niche shifts driven by interference competition: Giant hornets exclude rhinoceros beetles at sap sites at dawn.

Temporal niche shifts driven by interference competition: Giant hornets exclude rhinoceros beetles at sap sites at dawn.

Recognition of Bumblebee Behavior Based on AHP Model

We propose a vespa mandarinia recognition model based on analytic hierarchy process to assess and classify reports of sightings. we construct the analytic hierarchy process (AHP) model to evaluate and classify reports. The four main indicators in the AHP model extracted from the data are image, position, note and detection time, and the corresponding weights of the four indicators are calculated. Then, we establish models based on the four indicators to determine the scoring criteria. For the notes part, we use Term Frequency-Inverse Document Frequency arithmetic to extract keywords and Long Short-Term Memory (LSTM) model to classify and score the notes. Based on the AHP model, scores of the report can be calculated. Next, we use the scores of the given reports to calculate the classification threshold and divide all the report into three status based on two thresholds: positive, negative and unverified. According to the corresponding formula, the two thresholds are calculated as 0.6276 and 0.3937 respectively.

Evaluation of the Antimicrobial Properties of a Natural Peptide from Vespa mandarinia Venom and Its Synthetic Analogues as a Possible Route to Defeat Drug-Resistant Microbes.

Simple SummaryWith the prevalence of antimicrobial resistance caused by drug abuse, severe infection currently remains an urgent global challenge. The antimicrobial peptides rich in wasp venom advance the development of new drugs to addressing this issue. A natural peptide from wasp venom was discovered with a great antimicrobial activity against Gram-positive bacteria both in vitro and in vivo. Meantime, by adapting for specific substitution of amino acid, the engineered peptide exhibited more potency and broader spectrum of antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and yeast. Antimicrobial peptides (AMPs) from wasp venom have a good track record and potential for drug development as tools against development of antimicrobial resistance. Herein, the biological function and activity profile of peptide VM, which was discovered in the venom of the wasp, Vespa mandarinia, and several of its third-position substituted analogues, were investigated. VM had potent antimicrobial activity against Gram-positive bacteria and biofilm, and all modified peptides achieved the significant enhancement of these capacities. The various physicochemical properties of amino acids substituted in analogues, generated the different mechanisms of action of bacterial membrane disruption. VM-3K showed a maximum 8-fold enhancement of antibacterial activity against Gram-positive bacteria and also presented microbicidal properties against Gram-negative bacteria and fungi. This peptide also exhibited a high killing efficiency at low concentration and had a comparable selectivity index to VM. Furthermore, VM-3K produced a 90% survival of S. aureus-infected waxworms at a concentration of 5.656 mg/kg, at which concentration the natural template peptide only achieved 50% survival. This peptide also lacked short-term resistance generation. Thus, peptide VM-3K could be a promising broad-spectrum antimicrobial candidate for addressing the current antibiotic-resistant infection crisis. It is worth mentioning that this investigation on the relationship between peptide structure and mechanism of action could become an important aspect of drug research on short peptides.

First records of adventive populations of the parasitoids Ganaspis brasiliensis and Leptopilina japonica in the United States

We report the first known incidence of two parasitoid species of the invasive pest, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), in the United States (US). The discovery of Ganaspis brasiliensis (Ihering) (Hymenoptera: Figitidae) and Leptopilina japonica (Novković & Kimura) (Hymenoptera: Figitidae) in northwestern Washington State (US) was made shortly after their discovery in nearby southwestern British Columbia (Canada), indicating that contiguous populations of these species are established in both countries. The first specimen of L. japonica from Washington was collected in the fall of 2020, when it was found in a rice wine/orange juice trap deployed to survey for Vespa mandarinia Smith (Hymenoptera: Vespidae). Subsequent examination of trap contents from the 2020–2021 seasons indicated the presence of both L. japonica and G. brasiliensis. In September of 2021, live collections of both G. brasiliensis and L. japonica were made, reared from D. suzukii-infested Himalayan blackberry in Whatcom County, WA. Adult parasitoid identifications were based on morphology and COI DNA barcodes. All sequenced specimens to date from Washington and British Columbia belong to the G1 group of G. brasiliensis, the only group approved for release as a classical biological control agent in the US. This study provides an example of how even small changes in the geographic range of a natural enemy, now extending across an international border, may have significant consequences for the future of a biological control program.

First record of Vespa crabro Linnaeus (Hymenoptera: Vespidae) in western North America with a review of recorded species of Vespa Linnaeus in Canada.

Vespa crabro Linnaeus is newly reported as an adventive species in British Columbia, Canada which is the first record of this invasive species in western North America. The specimen of V. crabro was identified using morphological diagnostic keys and by comparison to authoritatively identified specimens. DNA barcoding provided support that the British Columbia specimen is conspecific with sequenced specimens of V. crabro. It is not possible to be certain of the origin of the specimen, but the DNA barcode was identical to sequence from specimens of V. crabro from South Korea. DNA barcoding was also performed on morphologically identified specimens of Vespa simillima and Vespa soror collected previously in British Columbia and the sequences were closest to V. simillima and V. soror Genbank sequences, respectively. There is no evidence that any of these species have established populations in the province. We provide diagnostic morphological characters to distinguish Canadian Vespa species from each other including Vespa mandarinia which has recently established populations in British Columbia and Washington State, USA. The potential detrimental impacts of each species are discussed.

Murder Hornet Mayhem: The Impact of the 2020 Giant Hornet Panic and COVID-19 Pandemic on Arthropod Identification Laboratories

Murder Hornet Mayhem: The Impact of the 2020 Giant Hornet Panic and COVID-19 Pandemic on Arthropod Identification Laboratories

N-Type Inactivation Variances in Honeybee and Asian Giant Hornet Kv Channels.

With the emergence of the Asian giant hornet as a threat to honeybee survival, knowledge of potential ion channel targets expressed in the nervous system can propel the development of new insecticides that are safe for pollinators. We therefore examined the biophysical properties of the Shaker-like voltage-gated potassium (Kv) channel of Apis mellifera (AmKv1; Western honeybee) and Vespa mandarinia (VmKv1; Asian giant hornet) and compared these data with isoforms that differ in N-terminal amino acid sequence. We expressed AmKv1 and VmKv1 in Xenopus laevis oocytes and determined their gating characteristics using electrophysiological measurements. Resulting features were compared with those gleaned from N-terminal isoforms. AmKv1 generates large potassium currents, but lacks an extended N-terminal region and therefore rapid N-type inactivation, as originally described in Shaker channels. Of its seven isoforms, two have a long N-tail and subsequently display inactivation. Notably, the isoform with the lengthiest N-terminal region only partially inactivates. VmKv1 potassium currents display N-type inactivation, as expected with an extended N-tail. One isoform shows an enhanced inactivation rate, whereas currents from another isoform with a substantially different N-terminal sequence could not be measured. AmKv1 and VmKv1 are functional Kv channels with strikingly different gating properties. Due to the presence of an extended N-terminal region, VmKv1 inactivates rapidly, whereas AmKv1 does not possess these residues and N-type inactivation is absent. Remarkably, virtually all isoforms of AmKv1 lack fast inactivation, whereas all studied VmKv1 isoforms inactivate, thereby suggesting a functional divergence that may be exploited for insecticide design.

A rat model of acute kidney injury caused by multiple subcutaneous injections of Asian giant hornet (Vespa mandarina Smith) venom

A model of acute kidney injury (AKI) caused by multiple subcutaneous injections of Asian giant hornet (Vespa mandarina Smith) venom was developed in male Wistar rats. The rats were injected subcutaneously at multiple sites in the dorsal region with a total venom dose of 5 mg/kg. Blood samples were obtained 8, 24 and 48 h after venom injection for the quantification of various biochemical parameters and assessment of renal function. Wasp venom caused significant increases in all biochemical parameters (aspartate aminotransferase, creatine kinase, free hemoglobin, lactate dehydrogenase, myoglobin, and plasma creatinine) within 8 h of venom administration, as well as marked alterations in renal function (a decrease in glomerular filtration rate leading to oliguria). The biochemical parameters showed varying degrees of recovery but remained elevated to varying degrees after 48 h, whereas the glomerular filtration rate showed no recovery during this period. The histopathological alterations consisted primarily of acute tubular necrosis, with tubular epithelial cell sloughing and loss of tubule brush border. This profile of biochemical and functional alterations was similar to that seen in human envenomations by this species, suggesting that this animal model may be useful for understanding the pathophysiology of AKI caused by this wasp venom.

Mapping a potential threat to the Mexican agricultural sector: The Asian Giant Hornet Vespa mandarinia (Smith 1852) (Hymenoptera: Vespidae)

The Asian giant hornet represents a threat to the global agricultural sector, as it is a species with predatory and invasive behavior that directly affects honey bees (Apis melifera), the main pollination agents of fruit trees, vegetables and seed production. The objective of the research was to develop a spatial diagnosis of the health risk associated with Vespa mandarinia Smith due to its possible impact on the Mexican agrifood sector. For this purpose, economically important crops were identified, grouped by pollination percentage and the potential distribution of the Asian giant hornet was identified using the maximum entropy model. The resulting health risk cartographic model allowed the identification of areas with different health risk indices (low, medium, high and very high), as well as the definition of priority areas spatially represented in quadrants and agri-food clusters at risk (clusters). These results are presented as a geotechnological tool that can strengthen the planning of epidemiological surveillance in Mexico, which has the potential to contribute to the establishment of preventive strategies to address the health risk posed by V. mandarinia to the Mexican agricultural sector.

Genes associated with hot defensive bee ball in the Japanese honeybee, Apis cerana japonica

BackgroundThe Japanese honeybee, Apis cerana japonica, shows a specific defensive behavior, known as a “hot defensive bee ball,” used against the giant hornet, Vespa mandarinia. Hundreds of honeybee workers surround a hornet and make a “bee ball” during this behavior. They maintain the ball for around 30 min, and its core temperature can reach 46. Although various studies have been conducted on the characteristics of this behavior, its molecular mechanism has yet to be elucidated. Here, we performed a comprehensive transcriptomic analysis to detect candidate genes related to balling behavior.ResultsThe expression levels of differentially expressed genes (DEGs) in the brain, flight muscle, and fat body were evaluated during ball formation and incubation at 46 °C. The DEGs detected during ball formation, but not in response to heat, were considered important for ball formation. The expression of genes related to rhodopsin signaling were increased in all tissues during ball formation. DEGs detected in one or two tissues during ball formation were also identified.ConclusionsGiven that rhodopsin is involved in temperature sensing in Drosophila, the rhodopsin-related DEGs in A. cerana japonica may be involved in temperature sensing specifically during ball formation.

Identification of giant hornet Vespa mandarinia queen sex pheromone components

The Vespidae is a diverse family of wasps and hornets that are formidable predators of insects, including social bees1, and includes a number of invasive species2. Recently, the world's largest hornet, Vespa mandarinia Smith (Hymenoptera: Vespidae), which occurs naturally in the Indomalayan region, has been found in Canada and the United States2. Some simulations indicate that it could rapidly spread throughout Washington and Oregon in the western US, as well as some eastern parts of the country2,3, threaten native bees and honeybees, and harm bee-pollinated crop production worth over $100 million annually3. There is consequently an urgent need to learn more about V. mandarinia's reproductive biology and to develop trapping methods to locate its nests and to control its reproduction. We identified V. mandarinia queen-produced sex pheromone from the 5th and 6th intersegmental sternal glands of virgin queens. The major active compounds were hexanoic acid, octanoic acid, and decanoic acid. When placed in field traps, the synthetic compounds and a queen-equivalent mixture rapidly attracted hundreds of males but no females or other species.

Assessment of an inexpensive trap design and survey method for vespine wasps (Hymenoptera, Vespidae, Vespinae)

The introduction of the predatory Giant Asian Hornet, Vespa mandarinia Smith, to North America in 2019 has motivated efforts to create early detection systems for this and other non-native social wasp species (Hymenoptera, Vespidae). Various trap and bait combinations have been used for this purpose, most of which require assembly and materials that are costly, reducing their usefulness in large-scale survey systems. This study tests an inexpensive and efficient trapping technique for detecting or surveying vespine wasps. Traps were made from reused plastic bottles containing a brown sugar and water bait. They were deployed at heights ranging from 0–6 m above ground in several configurations. Captures for traps suspended 1 m or greater above ground were, on average, nine times higher than the catch of ground-level traps. A rapid trap deployment method for large geographic areas was created, which captured seven different vespine wasp species along a 395 km east-west road transect from mountains to coastal plain in the Mid-Atlantic region of the United States. The trapping design and survey methodology described below is inexpensive and fast and could be used by land managers or citizen scientists to detect V. mandarinia, other exotic vespine, or conducted on a large-scale vespine diversity survey.

Utility of DNA barcoding for identification of common Vespa species (Hymenoptera: Vespidae) from Yunnan, China

AbstractAs traditional Chinese medicine and edible insects, Vespa hornets have high economic value and huge market demand. Hornet breeding has become an important way to increase the income of mountain farmers in China, but the limitation of traditional identification methods has become the bottleneck of the modern development of hornet resources. In this study, a simple and reliable method for the molecular identification of typical hornets from Yunnan was successfully established. In the NCBI database, DNA sequences of hornet samples were aligned as Vespa analis, Vespa basalis, Vespa velutina, Vespa mandarinia, Vespa magnifica, Vespa binghami, Vespa soror by BLAST search. The efficiency of the PCR amplification of mitochondrial cytochrome C oxidase I (COI) gene sequences in all samples was 100% by using the optimized program. Moreover, a reference database for DNA barcodes of Vespa materials in Yunnan was constructed. This study provided a successful example for the identification of local insects of Yunnan and will be helpful to the development of wasp resources.

Comparison of the External Morphology of the Sternal Glands for Hornets in the Genus Vespa.

Simple SummaryHornets are a group of 22 species of highly social wasps in the genus Vespa. These conspicuous insects construct large nests that are maintained by one or more queens and their offspring. Unlike other insects, many species of social wasps have glands on the underside of their abdomen called the van der Vecht and Richards glands. Documented uses for these glands by workers of some hornet species include producing pheromones for nestmate recruitment during group attacks on prey and chemical defense of nests against ants. We confirmed that these glands were present in nine hornet species. To examine differences among species in gland morphology, we used scanning electron microscopy to image the external surface of worker bodies around the glands. Accounting for differences in body size, we found that giant hornets and their close relatives had comparatively larger glands than the other species, some of which had glands that were approximately half as large. Differences among species are best explained by how hornets hunt and not by the likelihood that their nesting habitat increases exposure to ant predation. However, more information about how hornet workers use their sternal glands is necessary to permit further interpretation of their comparative gland morphology.Many social wasps in the speciose subfamilies Polistinae and Vespinae have two sternal glands—the van der Vecht gland and the Richards gland—that are not found in other insects. The presence of these glands has been confirmed in only 6 of 22 hornet species (genus Vespa) and images of their fine structure have not been produced. Here; we characterize the external morphology associated with both glands for workers of nine Vespa species using scanning electron microscopy. All hornets had similar gland configurations; although gland-associated external features differed among species. Scaled for size, glands were equivalently sized for the giant hornets (V. mandarinia and V. soror) and their closest phylogenetic relatives (V. tropica and V. ducalis). Relative size of gland-associated structures was reduced by half for V. simillima; V. velutina; and V. affinis workers. The remaining species (V. crabro and V. analis) had intermediately sized features. Differences among species in external gland structure were best explained by selective pressures related to predatory behavior, rather than defense of nests against ants. However, a lack of information about how Vespa workers use their van der Vecht and Richards glands limits a comparative interpretation of the function of their external gland morphology.

Hornet pests of honey bees in the Indian Himalayas and a low cost trapping device for their eco-friendly management

Honey bees suffer from a cosmic array of biotic and abiotic stress during their life cycle. The insect pests like wax moths and Dermestid beetles contribute immensely to the damage of bee colonies and reduction in honey yield in tropical regions of India. While, the bee colonies in temperate regions of Indian Himalayas, face an entirely different set of hitches that include predation of forager bees by three hornet species (Vespa mandarinia, Vespa velutina nigrithorax and Vespa tropica). The attacks on bee colonies by these hornet species compel the bees to desert the colony and swarm away to a new habitat thus, causing severe economic losses to bee keepers. Considering the severe setbacks enforced by hornets, a low-cost hornet trap was designed. The bait ingredients and their trapping efficiency against targeted hornet species are presented herewith. It was observed that green colored bottles attracted 34 hornets of three species in 10 days, while transparent bottles attracted only seven hornets at bait proportions of 1:2:2 (Apple cedar Vinegar: orange juice: water). Moreover, to reduce the attraction of honey bees into the trap, supplementary ingredients like rotten fruits and market honey were used, which enhanced the trapping efficiency of hornets. The benefit-cost ratio of 12.30:1 was recorded after the installation of the trap, which was significantly superior to traditional method (1.64:1) of manual swatting of hornets in the apiary. The farmers are currently trained to design and use these traps for the management of hornet pests in the Indian Himalayas.

Linking the Morphology of Sternal Glands to Rubbing Behavior by Vespa soror (Hymenoptera: Vespidae) Workers During Recruitment for Group Predation.

The activities of social insect colonies are supported by exocrine glands and the tremendous functional diversity of the compounds that they secrete. Many social wasps in the subfamilies Vespinae and Polistinae have two sternal glands—the van der Vecht and Richards’ glands—that vary in their features and function across the species in which they are found. Field observations suggest that giant hornets use secretions from the van der Vecht gland to chemically mark targeted nests when workers initiate group attacks on social insect prey. However, descriptions of giant hornets’ sternal glands and details about their recruitment behavior are lacking. We describe the morphology of the sternal glands of the giant hornet Vespa soror du Buysson and consider their potential to contribute to a marking pheromone. We also assess the gastral rubbing behavior of workers as they attacked Apis cerana F. (Hymenoptera: Apidae) colonies. V. soror workers have well-developed van der Vecht and Richards’ glands on their terminal gastral sternites, with morphologies that robustly support the synthesis, storage, and dissemination of their secretory products. Observations confirm that the van der Vecht gland is exposed during gastral rubbing, but that the Richards’ gland and glands associated with the sting apparatus may also contribute to a marking pheromone. Workers briefly but repeatedly rubbed their gasters around hive entrances and on overhead vegetation. Colonies were heavily marked over consecutive attacks. Our findings provide insight into the use of exocrine secretions by giant hornets as they recruit nestmates to prey colonies for group attacks.

Interspecific variation of antennal lobe composition among four hornet species

Olfaction is a crucial sensory modality underlying foraging, social and mating behaviors in many insects. Since the olfactory system is at the interface between the animal and its environment, it receives strong evolutionary pressures that promote neuronal adaptations and phenotypic variations across species. Hornets are large eusocial predatory wasps with a highly developed olfactory system, critical for foraging and intra-specific communication. In their natural range, hornet species display contrasting ecologies and olfactory-based behaviors, which might match to adaptive shifts in their olfactory system. The first olfactory processing center of the insect brain, the antennal lobe, is made of morphological and functional units called glomeruli. Using fluorescent staining, confocal microscopy and 3D reconstructions, we compared antennal lobe structure, glomerular numbers and volumes in four hornet species (Vespa crabro, Vespa velutina, Vespa mandarinia and Vespa orientalis) with marked differences in nesting site preferences and predatory behaviors. Despite a conserved organization of their antennal lobe compartments, glomeruli numbers varied strongly between species, including in a subsystem thought to process intraspecific cuticular signals. Moreover, specific adaptations involving enlarged glomeruli appeared in two species, V. crabro and V. mandarinia, but not in the others. We discuss the possible function of these adaptations based on species-specific behavioral differences.

Traditional rearing techniques of the edible Asian giant hornet (Vespa mandarinia Smith) and its socio-economic perspective in Nagaland, India

The larvae, pupae and adult of the world’s largest hornet Vespa mandarinia are a nutritious food popular among the different ethnic communities of Nagaland. The traditional method of collecting, rearing and consuming V. mandarinia still persists among the tribal people. Semi-domestication of V. mandarinia practiced in the region has been found to facilitate the production of hornets for socio-economic purposes; thereby contributing to conservation and a sustainable utilisation of the hornets. Eight to nine combs measuring 30-45 cm in diameter are usually harvested, each of which fetching an amount of Rs. 10,000-50,000. The present investigation provides data on the crude protein content of the larvae and pupae which amounts to 52.82 and 60.99%, respectively, suggesting that hornet grubs represent an important source of nutrition. While V. mandarinia are considered as pest in western countries and most of East Asia, the present study suggests hornets as a promising alternative food item rich in protein and that hornet rearing can improve the state of health and economic situation of Nagaland’s poorer section of the society.