Gestation Sows Research Articles

Conjugated linoleic acid supplementation during late gestation and lactation of sows affects myofiber type in their litters

The purpose of this study is to determine the effect of dietary conjugated linoleic acid (CLA) supplementation of pregnant and lactating sows on muscle growth and myofiber type of their piglets. There are thirty healthy, pregnant Rongchang sows with similar body condition and closed expected day of parturition for the experiment in vivo. Three experiment groups carry out with/without CLA (1%, 2% and 0%). The experiment began on 85th day of gestation and ended at 28th day after piglet birth. The results show that (1) piglets from CLA-fed sows had heavier longissimus dorsi and higher percentages of slow myofibers than control piglets (P<0.05); (2) moreover, real-time PCR showed that CLA supplementation induce increasing expression of MyHC 1, MyOG, and MyOD genes while decreasing expression of MyHC 2a, MyHC 2x, and MSTN genes in piglets. We also check the expression of genes which appear in 2 in vitro experiment. c9, t11-CLA acted to induce the differentiation of cultured pig skeletal muscle cells and up-regulates the expression levels of MyHC 1 and MyOG, but t10, c12-CLA strongly inhibited cellular differentiation and down-regulates expression of MyOG. These findings suggest that dietary CLA during early life may affect muscle development and myofiber type in pigs.

The role of the veterinary profession in promoting animal welfare.

JAVMA, Vol 246, No. 5, March 1, 2015 T beginning of the modern era of concern for animal welfare is often linked to the 1965 release of the Brambell report in the United Kingdom, which suggested that to ensure good welfare, an animal should have “sufficient freedom of movement to be able without difficulty to turn around, groom itself, get up, lie down and stretch its limbs.” Subsequently, the idea of the five freedoms (freedom from hunger and thirst; freedom from discomfort; freedom from pain, injury, and disease; freedom to express normal behavior; and freedom from fear and distress) emerged as a cornerstone of farm animal welfare policy in Great Britain. The five freedoms primarily focus on minimizing suffering and providing essential needs. A more comprehensive view of animal welfare that considers the perspective of the animal, rather than just the animal’s value to humans, has since been developed. This more contemporary interpretation takes into consideration not only the physical health (ie, whether the animals are free from pain, hunger, thirst, injury, and disease) of the animals under our supervision but also their mental health (ie, whether the animals suffer from the negative effects of stress and fear and whether they have opportunities for positive experiences such as play, touch, choice, or freedom) and ability to satisfy behavioral needs (ie, specific behavior patterns that when prevented, result in frustration or a negative psychological state). Veterinarians are in a unique position to influence animal welfare standards by virtue of our scientific training and the trust animal caretakers place in us. Concern for animal welfare prompted a revision of the veterinarian’s oath in 2010 to add the words and welfare: “I solemnly swear to use my scientific knowledge and skills for the benefit of society through the protection of animal health and welfare....” This acknowledged a change in the purview of the profession beyond that of physical health alone. Too often, however, the animal welfare policies adopted by the AVMA emphasize physical health to a greater extent than animals’ mental states or ability to express natural behaviors, particularly when it comes to farm animal welfare. I contend that these views on farm animal welfare lag behind those of society as a whole and that by continuing to espouse these positions, the AVMA risks abandoning its role as an advocate for the welfare of farm animals. An examination of just two issues—battery cage confinement of laying hens and confinement of breeding sows in gestation crates—illustrates my concerns. The role of the veterinary profession in promoting animal welfare

The effect of feeding a diet formulated for developing gilts between 70 kg and ~140 kg on lameness indicators and carcass traits

This study investigated the effect of three dietary regimes for replacement gilts on lameness and carcass traits. Diets were: a diet specifically formulated for replacement gilts (diet 1, 14.0MJ of DE/kg, 0.75% lysine), a finisher diet (diet 2, 13.5MJ of DE/kg, 1.02% lysine) and a gestation sow diet (diet 3, 13.0MJ of DE/kg, 0.69% lysine): the latter two diets are traditionally fed to replacement gilts. Thirty-six gilts were selected at day (d)0 (70.8kg±0.78 SE, aged ~130d), housed individually and allocated at random to 1) DEV (restricted access diet 1, n=12), 2) FIN (ad-libitum access diet 2, n=12) or 3) GES (initially ad-libitum access diet 2, then restricted access to diet 3 from d30, n=12). All gilts were fed ad-libitum from d71–83. Locomotory ability (0=normal to 5=severely impaired) and limb lesions (0=normal to 3=severe) were scored weekly until d82. Hind-claw lesions and toe uneveness were scored (0=normal to 3=severe) at d0, 40 and 82. Gilts were weighed at d0, 28, 70 and 82. Carcass traits were recorded at slaughter (d83) and one front leg was removed for dual-energy X-ray absorptiometry (DXA) to establish areal bone mineral density (aBMD). Joint surface lesions of the humeral condyle (HC; 1=normal to 4=severe, 5=osteochondrosis dissecans) and anconeal process (AP; 1=absent 2=present) were scored. The percentage of lame animals (locomotion score ≥2) on ≥1 occasion were DEV=0% (0/11), FIN=73% (8/11) and GES=75% (9/12) (P<0.01). Fewer DEV gilts than FIN and GES gilts had humeral condyle lesions: DEV=64% (7/11), FIN=100% (11/11) and GES=100% (12/12) (P<0.01). DEV gilts had lower scores for humeral condyle lesions (median; IQR: 2; 2) than GES (4; 1) and FIN gilts (4; 3) (P=0.05). DEV gilts weighed less (132.5kg±2.8) than FIN (142.9±2.0) gilts at d70 (P<0.05). DEV gilts had a lower average daily gain (ADG) than FIN (P<0.05) and GES (P<0.001) gilts at d0–28, lower energy intake than FIN and GES gilts from d0 to 28 (P<0.001) and higher energy intake than FIN gilts at d71–82 (P<0.05). The DEV diet formulated for replacement gilts and fed restrictively from 70kg to until ~130kg, then fed ad-libitum until ~140kg improved locomotory ability, toe eveness and humeral condyle joint lesions compared with traditional diets. It is likely these improvements were related to slower growth rates.

Environmental temperature effect on the gestation sows and related economic impacts

Este trabalho foi realizado junto a uma granja suinícola do Vale do Taquari, Rio Grande do Sul, com os objetivos de comparar a quantidade de leitões nascidos vivos por estação do ano, impactos econômicos relacionados e verificar a relação de perdas como retorno ao cio, abortos, teste de prenhez negativo (TPN) e mortes com a estação do ano (inverno e verão) durante a gestação fêmea suína. As matrizes suínas reprodutoras avaliadas pertencentes à genética Camborough, de ordem de parto variada, desde o primeiro até oito partos. Para verificação das amostras valeu-se da análise estatística descritiva e inferencial, apresentados em forma de médias (desvios padrão) e teste t para comparação dos grupos. Para correlacionar a média de temperatura do inverno e do verão com as perdas de leitões foi utilizada a correlação de Pearson. Observou-se, a partir dos dados analisados, que há diferença estatística significativa nas perdas (t = 4,3803; p = 0,0119), sendo a média de perdas maior no verão [188,4 (53,02)] do que no inverno [114,2 (17,01)]. Não há diferença estatística significativa na média de leitões mortos (mumificados ou natimortos) (t = -2.4572; p = 0,0698), no entanto, há diferença estatística significativa na média de leitões nascidos no verão [19.615,6 (995,16)] e no inverno [21.122,8 (1.421,18)] (t = -6.5361; p = 0,0028). Também se verificou que há correlação forte, positiva e significativa entre a média de perdas com a temperatura ambiente (r = 0,7575; p = 0,0111).

Effects of Dietary Supplementation of Oregano Essential Oil to Sows on Oxidative Stress Status, Lactation Feed Intake of Sows, and Piglet Performance.

Fifty-four multiparous large white sows were used to determine the effects of supplementing oregano essential oil (OEO) to the gestation and lactation diets on oxidative stress status, lactation feed intake, and their piglet performance. Two groups were fed diets with (OEO; n = 28) or without (Control; n = 26) supplemental 15 mg/kg OEO during gestation and lactation. The serum levels of reactive oxygen species (ROS) (P < 0.05), 8-hydroxy-deoxyguanosine (8-OHdG) (P < 0.05), and thiobarbituric acid reactive substances (TBARS) (P < 0.05) were higher during gestation (days 90 and 109) and lactation (days 1 and 3) than in early gestation (day 10). Compared with the control group, the OEO diet significantly reduced sows' serum concentrations of 8-OHdG (P < 0.05) and TBARS (P < 0.01) on day 1 of lactation. The OEO diet increased the sows' counts of faecal lactobacillus (P < 0.001) while reducing Escherichia coli (P < 0.001) and Enterococcus (P < 0.001). In the third week of lactation the treatment tended to increase sow's feed intake (P = 0.07), which resulted in higher average daily gain (P < 0.01) of piglets. Our results demonstrated that there is an increased systemic oxidative stress during late gestation and early lactation of sows. The OEO supplementation to sows' diet improved performance of their piglets, which may be attributed to the reduced oxidative stress.

Dietary betaine supplementation to gestational sows enhances hippocampal IGF2 expression in newborn piglets with modified DNA methylation of the differentially methylated regions.

The adequate supply of methyl donors is critical for the normal development of brain. The purpose of the present study was to investigate the effects of maternal betaine supplementation on hippocampal gene expression in neonatal piglets and to explore the possible mechanisms. Gestational sows were fed control or betaine-supplemented (3 g/kg) diets throughout the pregnancy. Immediately after birth, male piglets were killed, and the hippocampus was dissected for analyses. The mRNA abundance was determined by reverse transcription real-time polymerase chain reaction. Protein content was measured by Western blot, and DNA methylation was detected by methylated DNA immunoprecipitation assay. Prenatal betaine supplementation did not alter the body weight or the hippocampus weight, but increased the hippocampal DNA content as well as the mRNA expression of proliferation-related genes. Prenatal betaine supplementation increased serum level of methionine (P < 0.05) and up-regulated (P < 0.05) the mRNA and protein expression of betaine-homocysteine methyltransferase, glycine N-methyltransferase and DNA methyltransferase 1 in the neonatal hippocampus. Hippocampal expression of insulin growth factor II (IGF2) and its receptors IGF1R and IGF2R were all significantly up-regulated (P < 0.05) in betaine-treated group, together with a significant activation (P < 0.01) of the downstream extracellular signal-regulated kinase 1/2. Moreover, the differentially methylated region (DMR) 1 and 2 on IGF2 locus was found to be hypermethylated (P < 0.05) in the hippocampus of betaine-treated piglets. These results indicate that maternal betaine supplementation enhances betaine/methionine metabolism and DNA methyltransferase expression, causes hypermethylation of DMR on IGF2 gene, which was associated with augmented expression of IGF2 and cell proliferation/anti-apoptotic markers in the hippocampus of neonatal piglets.

A study on the Selection of Specific Behaviours of Pigs as a Measure of Animal Welfare Assessment

The purpose of this study was to selection of specific behaviours of pigs as a useful parameters for animal welfare assessment. Each specific behavioural patterns were divided the nomal and abnormal and observed in each step of pig(gestation, farrowing, nursery and grower-finisher) at conventional and welfare enriched farm. Conventional farm using the gestation stall, farrowing crate and nursery, grower-finisher pigs were accommodated small pen, while the welfare enriched farm using the group feeding gestation sows, farrowing pen without crate and nursery, grower-finisher pigs were accommodated large pen. Total 92 frequency of pig's behavioural patterns including 57 nomal and 35 abnomal behaviours were observed. The normal behavours of gestation sow was 14 patterns and abnormal behaviour was 8 patterns. Notable abnormal behaviours of gestation sow were vacuum chewing, bar biting and attack in the stall. Farrowing sow's abnormal behaviours selected the ear biting, cramp and attack. Abnormal behaviours selected in the nursery, grower-finisher pig was bothering, pushing, sucking, fighting and mounting.

Heat and Moisture Production Rates of a Modern U.S. Swine Breeding, Gestation, and Farrowing Facility

Abstract. Current recommendations for swine building ventilation system design to maintain an environment conducive to animal productivity and well-being are based on heat and moisture production rates measured in the 1950s and 1970s. Advancements in animal genetics, nutrition, and management practices to increase productivity and pork quality since then have led to considerable changes in heat and moisture production rates of modern swine and their housing systems. This study quantifies the total heat production rate (THP) of the animals, which is partitioned into house-level latent heat or moisture production rate (LHP, MP) and house-level sensible heat production rate (SHP), of a 4300-sow breeding, gestation, and farrowing facility in Iowa for 16 consecutive months. The THP was determined using indirect animal calorimetry, LHP or MP was determined from mass balance, and SHP was calculated as the difference between THP and LHP. A mobile air emission monitoring unit equipped with state-of-the-art gas analyzers and a data acquisition system was used to monitor the deep-pit breeding and early gestation barn [1800 head, 204 ±3.2 kg head -1 (mean ±SE)], the deep-pit late gestation barn (1800 head, 219 ±3.0 kg head -1 ), and two shallow-pit (pull-plug) farrowing rooms (40 sows with litters per room, 223 ±0.4 kg head -1 ). Results from the study show that THP at 20°C averages 1.89 W kg -1 for sows in the breeding and early gestation stage, 1.57 W kg -1 for sows in the late gestation stage, and 3.35 W kg -1 for sows and litters in week 0 of the lactation stage. The corresponding house-level LHP for the three stages averages 0.74 W kg -1 (early gestation), 0.57 W kg -1 (late gestation), and 1.98 W kg -1 (lactation, week 0). Finally, the corresponding house-level SHP for the three stages averages 1.15 W kg -1 (early gestation), 1.00 W kg -1 (late gestation), and 1.37 W kg -1 (lactation, week 0). Compared with the ASABE Standards, the values from the current study for gestation sows in their early and late pregnancy stages showed increases of 35% and 12% in THP, 72% and 34% in LHP, and 19% and 3% in SHP, respectively. Values for lactating sows and litters during the first week after parturition showed changes of 29% in THP, 52% in LHP, and 6% in SHP relative to the ASABE Standards. The reductions of THP from day to night for the three stages were 30% (early gestation), 27% (late gestation), and 6% (lactation). These data will help with updating the standards for ventilation design and operation of modern swine housing.

Maternal dietary betaine supplementation modifies hepatic expression of cholesterol metabolic genes via epigenetic mechanisms in newborn piglets.

To elucidate the effects of maternal dietary betaine supplementation on hepatic expression of cholesterol metabolic genes in newborn piglets and the involved epigenetic mechanisms, we fed gestational sows with control or betaine-supplemented diets (3g/kg) throughout pregnancy. Neonatal piglets born to betaine-supplemented sows had higher serum methionine concentration and hepatic content of betaine, which was associated with significantly up-regulated hepatic expression of glycine N-methyltransferase. Prenatal betaine exposure increased hepatic cholesterol content and modified the hepatic expression of cholesterol metabolic genes in neonatal piglets. Sterol regulatory element-binding protein 2 was down-regulated at both mRNA and protein levels, while 3-hydroxy-3-methylglutaryl CoA reductase (HMGCR) was down-regulated at the mRNA level, but up-regulated at the protein level, in betaine-exposed piglets. The transcriptional repression of HMGCR was associated with CpG island hypermethylation and higher repressive histone mark H3K27me3 (histone H3 lysine 27 trimethylation) on the promoter, whereas increased HMGCR protein content was associated with significantly decreased expression of miR-497. Furthermore, LDL receptor was significantly down-regulated at both mRNA and protein levels in the liver of betaine-exposed piglets, which was associated with promoter CpG hypermethylation. In addition, the expression of cholesterol-27α-hydroxylase (CYP27α1) was up-regulated at both mRNA and protein levels, while the expression of cholesterol-7α-hydroxylase (CYP7α1) was increased at the mRNA level, but unchanged at the protein level associated with increased expression of miR-181. These results indicate that maternal betaine supplementation increases hepatic cholesterol content in neonatal piglets through epigenetic regulations of cholesterol metabolic genes, which involve alterations in DNA and histone methylation and in the expression of microRNA targeting these genes.

Betaine supplementation in maternal diet modulates the epigenetic regulation of hepatic gluconeogenic genes in neonatal piglets.

In this study, gestational sows were fed control or betaine-supplemented diets (3 g/kg) throughout the pregnancy, and the newborn piglets were used to elucidate whether maternal dietary betaine affected offspring hepatic gluconeogenic genes through epigenetic mechanisms. Neonatal piglets born to betaine-supplemented sows had significantly higher serum and hepatic betaine contents, together with significantly greater expression of methionine metabolic enzymes in the liver. Interestingly, significantly higher serum concentrations of lactic acid and glucogenic amino acids, including serine, glutamate, methionine and histidine, were detected in the piglets born to betaine-supplemented sows, which were coincident with higher hepatic glycogen content and PEPCK1 enzyme activity, as well as greater protein expression of gluconeogenic enzymes, pyruvate carboxylase (PC), cytoplasmic phosphoenolpyruvate carboxykinase (PEPCK1), mitochondrional phosphoenolpyruvate carboxykinase (PEPCK2) and fructose-1, 6-bisphosphatase (FBP1). Moreover, maternal betaine significantly changed the methylation status of both CpGs and histones on the promoter of gluconeogenic genes. The lower PEPCK1 mRNA was associated with DNA hypermethylation and more enriched repression histone mark H3K27me3, while the up-regulated PEPCK2 and FBP1 mRNA was associated with DNA hypomethylation and more enriched activation histone mark H3K4me3. Furthermore, the expression of two miRNAs predicted to target PC and 6 miRNAs predicted to target PEPCK1 was dramatically suppressed in the liver of piglets born to betaine-supplemented sows. Our results provide the first evidence that maternal betaine supplementation affects hepatic gluconeogenic genes expression in newborn piglets through enhanced hepatic methionine metabolism and epigenetic regulations, which involve DNA and histone methylations, and possibly miRNAs-mediated post-transcriptional mechanism.

Effects of magnesium on the performance of sows and their piglets.

The objective of this study was to evaluate the effects of supplemental magnesium (Mg) on the performance of gilts and parity 3 sows and their piglets. Fifty-six gilts (Trial 1) and 56 sows (Trial 2) were assigned to one of 4 treatments according to their mating weight, respectively. The treatments comprised corn-soybean meal based gestation and lactation diets (0.21% magnesium) supplemented with 0, 0.015, 0.03, or 0.045% Mg from mating until weaning. The results showed that magnesium supplementation significantly (P < 0.05) reduced the weaning to estrus interval in both gilts and sows. There were significant effects (P < 0.05) of supplemental magnesium on the total number of piglets born, born alive and weaned in sows. In late gestation and lactation, the digestibility of crude fiber (quadratic effects, P < 0.05), and crude protein (P < 0.05), were significantly influenced by magnesium in gilts and sows, respectively. There were differences among the 4 groups in terms of the apparent digestibility of dry matter and crude fiber in sows (P < 0.05) during both early and late gestation. The apparent digestibility of gross energy was increased for sows in late gestation (P < 0.05), and lactation (quadratic effects, P < 0.05). At farrowing and weaning, serum prolactin levels and alkaline phosphate activities linearly increased in sows as the Mg supplementation increased (P < 0.05). Serum Mg of sows at farrowing and serum urea nitrogen of sows at weaning was significantly influenced by Mg supplementation (P < 0.05). The Mg concentration in sow colostrum and the serum of their piglets were increased by supplemental magnesium (P < 0.05). In addition, growth hormone levels were linearly elevated (P < 0.05) in the serum of piglets suckling sows. Our data demonstrated that supplemental magnesium has the potential to improve the reproduction performance of sows, and the suitable supplemental dose ranged from 0.015% to 0.03%.

TRIENNIAL GROWTH SYMPOSIUM— Effects of dietary 25-hydroxycholecalciferol and cholecalciferol on blood vitamin D and mineral status, bone turnover, milk composition, and reproductive performance of sows12

To evaluate the role of vitamin D3 during gestation and lactation of sows, 2 independent experiments were performed with the aim of investigating sow reproductive performance, milk composition (study 1 only), and changes in blood status of 25-hydroxycholecalciferol (25-OH-D3), 1,25-dihydroxycholecalciferol (1,25-(OH)2-D3; study 2 only), minerals, and bone markers of sows during gestation and lactation. Study 1 comprised 39 primi- and multiparous crossbred sows fed 1 of 3 barley meal-based diets fortified with 200 IU/kg vitamin D3 (NRC, 1998; treatment DL), 2,000 IU/kg vitamin D3 (cholecalciferol; treatment DN), or 50 μg 25-OH-D3 (calcidiol; treatment HD)/kg feed. This study was conducted over a 4-parity period under controlled conditions. Study 2, running over 1 parity only, was performed in a commercial farm with 227 primi- and multiparous sows allocated to 2 dietary treatments: control (CON), receiving 2,000 IU vitamin D3/kg (equivalent to 50 μg/kg) feed (114 sows), and test (HYD), supplemented with 50 μg 25-OH-D3/kg feed (113 sows). Blood samples of sows were collected at 84 and 110d postcoitum and 1, 5, and 33 d postpartum (study 1) and at insemination and 28 and 80 d postinsemination as well as d 5 and 28 postpartum (study 2). Colostrum and milk samples in study 1 were obtained at 1, 9, and 33 d of lactation after oxytocin administration. Plasma 25-OH-D3 concentrations were increased (P < 0.05) in sows receiving 25-OH-D3 (HD and HYD) at any time of sampling whereas circulating plasma concentrations of 1,25-(OH)2-D3, Ca, and P were not affected by treatment. Milk concentrations of Ca and P were similar, but 25-OH-D3 content (except in colostrum) was clearly increased (P< 0.05) when 25-OH-D3 was fed. Most characteristics of sow reproductive performance responded similarly to the 2 sources and levels of vitamin D3, but weight gain of piglets between birth and weaning was decreased (P< 0.05) in offspring of DL and HD sows compared with animals of treatment DN (study 1). In study 2 total litter weight and birth weight per piglet were increased (P< 0.05) with 25-OH-D3 supplementation in comparison with the control (CON). Overall, feeding sows with 25-OH-D3 was considered to improve maternal supply with vitamin D3 and thereby maintain Ca homeostasis during gestation and lactation.

Herd- and sow-related risk factors for lameness in organic and conventional sow herds

Lameness in sows is an animal welfare problem which also presents an economic challenge to pig producers. Information about the prevalence of herd lameness in organic sows is relatively scarce. The first objective of this study was to establish the prevalence of lameness and to identify risk factors associated with sow lameness in Danish outdoor organic sow herds by analysing the association between risk factors at both sow and herd level using clinical records of lameness. A total of 1850 sows from nine organic herds were included in the study. Second, the study examined differences in the prevalence of sow lameness between outdoor organic and indoor conventional herds. An additional aim here was to identify risk factors associated with clinical records of sow lameness in Danish sow herds by analysing the association between risk factors with lameness at sow and herd level. One thousand and fifty four gestation sows from 44 indoor conventional and nine organic sow herds were included in this study. The nine organic herds were visited twice: once in summer/autumn 2011, and once in winter/spring 2012. In winter/spring 2011, a total of 44 indoor conventional herds were visited. Risk factors included in the study were clinical parameters and factors related to the production system. Sows were examined visually by one of four trained observers. The organic sows were assigned scores for lameness, body condition, hoof length, bursitis, abscesses and leg wounds, while the conventional sows were assigned scores for lameness, body condition and bursitis. A multivariable analysis was carried out by logistic regression with the herd and observer as random effects. The average herd lameness prevalence in gestation and lactation sows in organic herds was 11% in summer/autumn and 4.6% in winter/spring. ‘Wounds, bursitis and abscess’ on legs (OR=4.7, P<0.001) and body condition score >3 (OR=1.79, P=0.008) were associated with increased risk of lameness in Danish organic sow herds. Season (winter/spring v. summer/autumn) lowered the risk of lameness (OR=0.37, P<0.001). Average prevalence of lameness in gestation sow herds in winter/spring in conventional herds was 24.4%, and in organic herds it was 5.4%. An organic sow had a decreased risk of lameness (OR=0.28, P<0.001) as compared with a conventional sow. Bursitis was associated with increased risk of lameness (OR=2.08, P=0.002) regardless of the production system (i.e. whether the herd was organic or conventional).

임신돈 군사장치의 개발

The study was conducted to develop an ESF (electronic sow feeder) for improving animal welfare of group housed gestation sows. Feed intake of both individual and group housed gestation sows was restricted 2 kg a day. ESF system was very effective in reducing feed loss. The amount of feed loss in treatment group (group housed gestation sows with ESF) was only about 55 g, while the amount of feed loss in control group (individually housed gestation sows) was about 200 g. In spend time to feed intake, there was no difference in the result between the control (about 30 min) and the treatment (about 22 min) group. The ESF system ran well without any mechanical problems throughout the entire experiment. These results suggest that the EST is expected to improve the performance feeding of sows.

Impact of diet deprivation and subsequent overallowance during gestation on mammary gland development and lactation performance1

The impacts of diet deprivation and subsequent overallowance during gestation on mammary gene expression and development and lactation performance were determined. Gilts were reared under a conventional (control, CTL; n = 59) or an experimental (treatment, TRT; n = 56) dietary regimen during gestation. The experimental regimen provided 70% (restriction diet, RES) and 115% (overallowance diet, OVER) of the protein and DE contents provided by the CTL diet. The RES diet was given during the first 10 wk of gestation followed by the OVER diet until farrowing. Some gilts (14 CTL and 14 TRT) were slaughtered on d 110 of gestation, and the others were allowed to farrow. Of these remaining sows, 28 (14 CTL and 14 TRT) were slaughtered on d 21 of lactation, and the rest underwent a second lactation. At each slaughter, mammary tissue was collected for compositional analyses and assessment of gene expression. Milk samples were collected on d 17 of the first lactation. Litter size was standardized to 11 ± 1, and piglets were weighed weekly until d 18 in both parities. The BW and back fat thickness of TRT first-parity sows were less than those of CTL sows in gestation (P < 0.05), and their BW was also less in lactation (P < 0.05). The BW of TRT second-parity sows was still less at mating (P < 0.05) and tended to be less on d 1 of lactation (P < 0.10) compared with CTL sows. There were no differences in piglet growth between CTL and TRT litters in either parity, yet mammary development and mammary gene expression were affected by treatment. There was less parenchymal tissue (P < 0.01) at the end of the first gestation in TRT than in CTL sows, but parenchymal tissue composition was not altered by treatment. Relative abundance of IGF-1 (P < 0.05), ornithine decarboxylase (P < 0.05), signal transducer and activator of transcription 5B (P < 0.05), and whey acidic protein (WAP, P < 0.01) genes in parenchyma at the end of the first gestation was lower in TRT than in CTL sows, and the effect on WAP genes was still present at the end of the first lactation (P < 0.01). Mammary composition at the end of the first lactation and milk composition were unaffected by treatment. In conclusion, feed deprivation and subsequent overallowance in gestation had unfavorable effects on sow BW, back fat, mammary development, and mammary gene expression at the end of gestation, but piglet growth rate over the 2 parities was not affected.

Attitudes of Canadian Pig Producers Toward Animal Welfare

As part of a larger study eliciting Canadian producer and non-producer views about animal welfare, open-ended, semi-structured interviews were used to explore opinions about animal welfare of 20 Canadian pig producers, most of whom were involved in confinement-based systems. With the exception of the one organic producer, who emphasized the importance of a “natural” life, participants attached overriding importance to biological health and functioning. They saw their efforts as providing pigs with dry, thermally regulated, indoor environments where animals received abundant feed, careful monitoring and where prospective disease outbreaks could be minimized and controlled. Emphasis was also placed on low-stress handling and agreeable working conditions which were believed to promote good animal care. The fact that pigs tend to respond to such conditions with steady growth reinforced the belief that good welfare was provided. Participants supported the use of sow gestation stalls, but with some reservations, and expressed concern about welfare problems that could occur if sows were grouped. Invasive procedures (castration, tail-docking, teeth clipping) were recognized as painful but were accepted because they were seen as: (1) necessary for sales or management; (2) satisfactory trade-offs to prevent worse welfare problems such as injury or infection; or (3) sufficiently short-term to be relatively unimportant. Participants were adamantly opposed to animal neglect and some welcomed actions of animal protectionists that expose poor care. Producers also welcomed natural-science-based approaches to improving animal welfare. The findings contribute to a broader effort to identify overlapping values among different stakeholder groups as a basis for formulating mutually agreeable, farm animal care and handling polices.

Improving efficiency of sow productivity: nutrition and health

This reviews research focused to understand the nutrient requirement and balance to meet the needs of fetal growth, mammary growth, and milk production. This summary will handle how feeding strategies can be adjusted according to the nutrient needs for a sow to enhance productivity and health. Most research data used in this summary are based on the studies conducted by the authors between 1996 and 2013. Nutrient requirements of sows are affected by stage of gestation and parity of sows. Dietary antioxidant concentrations need to be re-evaluated for its sufficiency in sow diets especially to prevent excessive oxidative stress during late gestation and lactation. When feeding sows, consideration of phase feeding of gestating sows and parity feeding of lactating sows could enhances production longevity and health of sows. Use of selected nutrients and additives seems to help productivity and health of sows.

Effect of increasing lysine supply during last third of gestation on reproductive performance of Iberian sows

Ninety purebred Iberian (IB) sows in second or third parity were used to determine the effects of dietary lysine (Lys) concentration during last third of pregnancy on sow and litter performance. The sows were randomly assigned to one of three dietary treatments: 5.5 (LLys, Control), 7.4 (MLys) and 8.7 (HLys) g Lys kg-1 diet. Feed allowance was 2.30-2.33 kg d-1. Close to farrowing a conventional lactation diet was used. Dietary Lys did not affect body-weight (BW) gain in late gestation of second-parity sows. However, in third-parity sows, a strong tendency was observed for BW gain to increase during late pregnancy with dietary Lys levels higher than 5.5 g kg-1 (p=0.061). Body-weight losses during lactation were never influenced by Lys supply. A strong tendency (p=0.064) for a lower ratio between piglets born alive and total piglets born was observed in second-parity sows fed the Control gestation diet. Litters and piglets from sows on this diet had the lowest weight at birth (p0.05). In conclusion, under moderate energy supply, adequate reserves for subsequent lactation can be achieved in second- and third-parity IB sows with a daily provision of at least 17.2 g lysine over the last third of pregnancy, what implies a substantial increase in protein supply respect to traditional practices.

Effect of gestating sow body condition, feed refusals, and group housing on growth and feed intake in grower–finishing pigs1

The main focus of this study was to identify sow gestation features that affect growth rate (GR) and feed intake (FI) of their offspring during grower-finishing stage. Because the sow provides a specific environment to her offspring during gestation, certain features (e.g., BW of the sow), feed refusals or gestation group, may affect her ability to deliver and feed a healthy litter. Data on 17,743 grower-finishing pigs, coming from 604 sires and 681 crossbred sows, were obtained from the Institute for Pigs Genetics. Sow gestation features were collected during multiple gestations and divided into 3 clusters describing i) sow body condition (i.e., BW, backfat, and gestation length), ii) sow feed refusals (FR), the difference between offered and eaten feed during 3 periods of gestation: 1 to 28, 25 to 50, 45 to 80 d, and iii) sow group features (i.e., number of sows, and average parity). Sow gestation features were added to the base model 1 at a time to study their effect on GR and FI. Significant gestation features (P < 0.1) were fitted simultaneously in animal model to investigate whether they could explain common litter and permanent sow effects. Gestation length had effect on GR [1.4 (g/d)/d; P = 0.04] and FI [6.8 (g/d)/d; P = 0.007]. Body weights of the sow at insemination [0.07 (g/d)/kg; P = 0.08], at farrowing [0.14 (g/d)/kg; P < 0.0001], and after lactation [0.1 (g/d)/kg; P = 0.003] had effect on GR. Sow parturition-lactation loss in backfat thickness and weight were not significant for GR and FI. Days with FR during 25 to 50 and 45 to 80 d of gestation and average FR during 45 to 80 d of gestation had negative effect on GR and when substantially increased had also a positive effect on FI. Sow FR from 1 to 28 d of gestation were not significant. Number of sows in gestation group had effect on FI [-9 (g/d)/group member; P = 0.04] and day sow entered group had an effect on GR [-0.9 (g/d)/day; P = 0.04]. Sow gestation features explained 1 to 3% of the total variance in grower-finishing pigs. Gestation features did explain phenotypic variance due to permanent sow and part of phenotypic variance due to common litter effects for FI but not for GR.

The Effects of Dietary Supplementation with Chromium Picolinate throughout Gestation on Productive Performance, Cr Concentration, Serum Parameters, and Colostrum Composition in Sows

The objective of this study was to determine the effects of supplemental chromium as chromium picolinate (CrPic) on productive performance, chromium (Cr) concentration, serum parameters, and colostrum composition in sows. Thirty Yorkshire sows were bred with semen from a pool of Landrace boars. The sows were equally grouped and treated with either a diet containing 0 (control) or 400 ppb dietary Cr supplementation throughout gestation. The sows received the same basal diet based on corn-DDGS meal. Supplemental CrPic increased (P < 0.05) the sow body mass gain from the insemination to the day 110 of gestation in sows. No differences (P > 0.50) were observed in the gestation interval, sow mass, and backfat at insemination, after farrowing, at weaning and lactation loss. The number of piglets born alive, piglets per litter at weaning, and litter weaned mass were increased (P < 0.05) for those supplemented with CrPic compared with the control. However, the total number of piglets born, total born litter mass, average piglet birth body mass, born alive litter mass, and average born alive piglet mass did not differ among the treatments (P > 0.05). The placental masses of sows were similar among treatments (P > 0.05). Dietary supplementation with CrPic throughout gestation in sows showed increased (P < 0.01) concentration of Cr in the colostrum or serum at days 70 and 110. Compared with the control group, dietary supplementation with CrPic throughout gestation in sows decreased (P < 0.05) the serum insulin concentration, the glucose or serum urea nitrogen concentration at days 70 and 110. However, no differences (P > 0.05) were observed in total protein concentration among treatments. No differences (P > 0.05) were observed in total solids, protein, fat or lactose among sows fed the diets supplemented with CrPic compared with the control. This exciting finding provides evidence for an increase in mass gain and live-born piglets in sows supplemented with CrPic throughout gestation.