Event Abstract Back to Event Effect of the 3’Regulatory Region insulation on B cell development and DNA rearrangements at the Immunoglobulin heavy chain locus Fatima-Zohra Braikia1, Mohamed Moutahir1, Caroline Conte1* and Ahmed A. Khamlichi1 1 University of Toulouse, CNRS UMR5089/University of Toulouse, France The Immunoglobulin heavy chain locus (IgH) undergoes a series of developmentally-regulated DNA rearrangements, named V(D)J recombination. Regulation of these events is effected by various cis-acting elements among which the Eµ enhancer plays a major role. Another important cis-element is the 3’ Regulatory Region, located downstream of the IgH locus, and which was suggested to interact with Eµ enhancer. CTCF-binding elements have been described at the IgH locus and are thought to mediate the formation of large loops that may be important for various processes linked to V(D)J recombination. In this study, we have adopted an insulation approach by inserting the well-characterized cHS4 insulator of the chicken beta-globin locus, upstream of the IgH 3’RR. We generated two mouse lines in which either the wild-type, CTCF-binding, cHS4 insulator was used or a mutated version of it devoid of CTCF-binding site. We will present our recent results on the long-range effect of 3’RR insulation on B cell development, V(D)J recombination, allelic exclusion and IgH expression. Acknowledgements This work was funded by INCa (PLBIO11-181), ANR (ANR-07-BLAN-0080-03), ARC (Grant SFI20101201441), LNCC-Comité de Haute-Garonne, Cancéropôle GSO, and the University of Toulouse Keywords: B cell development, germline transcription, IgH enhancer, VDJ, CSR Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune receptors and signaling Citation: Braikia F, Moutahir M, Conte C and Khamlichi AA (2013). Effect of the 3’Regulatory Region insulation on B cell development and DNA rearrangements at the Immunoglobulin heavy chain locus. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00251 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Caroline Conte, University of Toulouse, CNRS UMR5089/University of Toulouse, Toulouse, France, caroline.conte@ipbs.fr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Fatima-Zohra Braikia Mohamed Moutahir Caroline Conte Ahmed A Khamlichi Google Fatima-Zohra Braikia Mohamed Moutahir Caroline Conte Ahmed A Khamlichi Google Scholar Fatima-Zohra Braikia Mohamed Moutahir Caroline Conte Ahmed A Khamlichi PubMed Fatima-Zohra Braikia Mohamed Moutahir Caroline Conte Ahmed A Khamlichi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.