Germination Speed Research Articles

Comparative study of salt tolerance between two soybean varieties (Glycine max. L) at germination stage

In December 2019, a research work on seed germination was carried out in Plant Physiology and Biochemistry Laboratory, Jahangirnagar University, to determine the salt tolerance level and compare the salt stress response between two soybean varieties at early seedling stage. Seeds of soybean varieties viz Shohag and BARI Soybean-5 were grown in Petri dishes and treated with 0, 50, 100, 150, 200, 250, and 300 mM NaCl for 14 days. Salinity had adverse effects on germination percentage, germination index, germination speed and growth parameters viz shoot length, root length, fresh weight of shoots, fresh weight of roots and dry matter production in shoot and root for early seedling growth. The results illustrate that germination percentage and germination index of Shohag were more than 80% at up to 200 mM NaCl concentration, while both of these variables for BARI Soybean-5 were more than 80% at up to 100 mM NaCl treatments. Although the germination speed of BARI Soybean-5 was faster than Shohag at moderate salinities, it was much slower for BARI Soybean-5 than Shohag at high salinities. Results showed that the growth of both varieties slowed significantly with increased salinity. However, Shohag outperforms BARI Soybean-5 in terms of growth at high salt concentration. As a result, it can be stated that BARI Soybean-5 can tolerate moderate salt. It can be hypothesized that Shohag could be used for field experiments due to its superior germination performance. Jahangirnagar University J. Biol. Sci. 12(1 & 2): 35-44, 2023 (June & December)

DEVELOPMENTAL CONTROL OF SOME PHYSIOLOGICAL FACTORS ON REPRODUCTIVE BIOLOGY AND RUDIMENTARY EMBRYOS PHENOMENON IN CARROT SEEDS

This study was aimed to investigate delayed germination and immature embryos of carrot seeds; This research was implemented at vegetable field of College of Agricultural Engineering Sciences - University of Baghdad during two sequent seasons (fall and spring). The experiment was conducted using factorial within Randomized Complete Block Design arrangement with three factors and replicates (3X3X2). The aqueous barley sprouts extract (B0, B1) (0, 100 g.L-1) represented the first factor. Trehalose (T0, T1, T2) (0, 50, 75 mmole.L-1) represented the second factor. Calcium (C0, C1, C2) (0, 1, 2 ml.L-1) represented the third factor. Results showed the effectiveness of third order interaction treatment B1T2C1 in increasing germination speed and percent for the seeds of royal umbel (92, 97%); secondary umbel (90.67, 94.67%); and tertiary umbel (75.68, 86.33%). The mentioned treatment increased embryo length for the seeds of primary (royal) umbel (1628.3 µm), secondary umbel (1620.3 µm); and tertiary umbel (874.7 µm ). in compare with the lowest percents that found in B0T0C2 treatment.

PHYSIOLOGICAL PERFORMANCE OF SEEDS OF Phaseolus vulgaris L. AS A FUNCTION OF ZINC AND PHYSIOLOGICAL MATURITY

The objective of this study was to study the physiological quality of bean treated with levels of zinc during the physiological maturation stage. The work was carried out at Fazenda Ponte da Braúna and at the Seed Analysis Laboratory of the Center for Agricultural Sciences and Engineering at the Federal University of Espírito Santo. Bean seeds of the cultivar Peanut were treated with Zn at concentrations of 0; 2.5; 5.0; 7.5 and 10 g kg-1 of seeds and sown in the field. The variables analyzed were seed moisture, 1000-seed weight, length, width and thickness of pods and seeds, germination, germination speed index, shoot length, root length, shoot and root dry matter. The experimental design used was completely randomized, in the factorial 5 x 7 (zinc doses x maturation periods), with four replications of 25 seeds. The seeds of the bean cultivar Amendoim treated with zinc at 10 g kg-1 seeds showed the highest values of pod length, thickness, and width. The seeds of the bean cultivar Amendoim showed the highest germination means and GSI after 35 days from anthesis. The treatment of seeds of the bean cultivar Amendoim with zinc does not influence the seedling characteristics. Seed treatment with zinc does not interfere with the seed maturation time of the bean cultivar Amendoim. The physiological maturation of seeds of the bean cultivar Amendoim occurs after 42 days from anthesis. The seed water content of the bean cultivar Amendoim at the stage of physiological maturation is 19%

Influence of seed priming in the germination and yield performances of common buckwheat

The study, conducted from November 2019 to March 2020 at the Institute of Agriculture and Animal Science in Rupandehi, Nepal, aimed to evaluate the effects of seed priming techniques on common buckwheat. The experiment comprised eleven treatments with three replications each. Germination parameters were analyzed in the laboratory using a complete block design, while growth and yield parameters were assessed in the field using a randomized complete block design. The results indicated significant improvements in all germination and yield parameters of buckwheat due to seed priming. Notably, the 48-hour hydropriming treatment exhibited the highest performance, yielding a germination percentage of 87.167%, a speed of germination of 66.62, a vigor index of 52.78, a grain yield of 1518.988 kgha-1, a test weight of 19.533 g, and a harvest index of 0.405. These findings suggest that priming seeds with water for 48 hours can effectively enhance the germination, growth, and yield attributes of common buckwheat.

Hot and fast: seed ecology for restoration relevant species in the Argyle region of the east Kimberley, Australia

This study investigates the germination requirements of 12 plant species native to the Argyle region of the east Kimberley, a biodiverse monsoonal tropical region characterized by high temperatures, high evaporation, and episodic seasonal rainfall. The research involved quality assessment of mature seeds, followed by dormancy alleviation and laboratory‐based germination to determine the responses of seeds to a range of temperatures (5–40°C) in terms of germination speed (T10), mean germination time, and maximum germination proportion. Data were then modeled to calculate the optimal temperature to support germination for each species. The results showed that germination rapidly commences in response to a wide range of temperatures typical of the wet season (November–February) in the east Kimberley, though germination for most species was still high (>50%) after exposure to temperatures as low as 15°C. Mean optimal temperature for germination across all species was 25.8 ± 1.5°C, with minimal variation between most species, the exception being Dodonaea physocarpa, which preferred cooler temperatures (Topt = 14.0°C). The speed of germination was also rapid (T10 = 1–3 days) across all species at the optimal germination temperature. The findings suggest that temperature is not a limiting factor for germination in this region and that the onset and intensity of the wet season are the most significant factors determining successful germination, emergence, and seedling establishment. The study underscores the importance of species‐specific understanding of environmental temperatures required for seed germination in seed‐based restoration efforts and informs the planning of direct seeding works, thus enhancing restoration outcomes.

Chemical and Mechanical Scarification as Techniques for Overcoming Dormance in Red Tent Seeds (Adenanthera pavonina L.)

Purpose: Evaluate chemical and mechanical scarification techniques, as methods for overcoming dormancy in red vine seeds. Theoretical Framework: Based on scientific articles found on search engines and books in the field of seeds, types of forest seeds, types of dormancy in forest seeds and techniques for overcoming dormancy. In order to support the explanation of how techniques for overcoming dormancy are related to the rate and time of germination of red seed seeds, and how germination time can subsequently affect the quantity of seedlings produced, as well as their initial growth. Method: The experiment was installed to test the following treatments: T0 – without scarification; T1 – mechanical scarification; T2 chemical scarification with 80% H2SO4 for half an hour; T3 – chemical scarification with 80% H2SO4 for one hour; T4 – chemical scarification with 0.4% KNO3 for half an hour; T5 – chemical scarification with 0.4% KNO3 for one hour. The design used was completely randomized, with six treatments, five replications and 20 seeds in each replication. At the end of 30 days, counting from the beginning of germination, the following variables were evaluated: germination speed index (IVG), average germination time (TMG), germination percentage (G) and germination speed index (IVG). Results and Conclusion: The most efficient methods for germinating the red tent seeds were mechanical scarification with sandpaper and immersion in 0.4% KNO3 for half an hour. However, mechanical scarification would be the most easily used and least expensive method for producing seedlings in nurseries. Research Implications: These results indicate that the mechanical scarification technique is an efficient method for overcoming the dormancy of the red tent, which is integumentary. Understanding the most efficient and effective techniques for overcoming dormancy in seeds of species that present this characteristic is important to allow, within the nursery, germination to occur quickly and uniformly. Consequently, making it possible to obtain seedlings with more uniform sizes, even though these are produced from seeds. Originality/Value: A high germination rate and uniform initial growth are important factors within the nursery, especially if it is of a commercial nature, but also for the restoration of areas, because it allows seedlings to be obtained in less time.

Quality assessment of soybean seeds submitted to industrial seed treatment and stored in a natural and controlled environment

Seed quality is a limiting factor in the search for high soybean yields. However, fungi and insect pests can negatively interfere with their performance, and industrial seed treatment (IST) is a tool used to minimize the attack of pathogens and pests. The treated seeds are often stored until sowing, which can lead to a reduction in their quality. This work aimed to evaluate the physical and physiological quality of soybean seeds submitted to IST, with subsequent storage in a natural and controlled environment. The experiment was conducted in a split-split-plot design in a completely randomized scheme (time x storage systems x IST) with four replications. Soybean cultivar 55I57RSF IPRO was subjected to IST with fungicide (metalaxyl-M, thiabendazole, and fludioxonil) and insecticide (thiamethoxam and cyantraniliprole), combined or not with polymer and dry powder, with subsequent storage in a controlled and natural environment. The moisture content, thousand-seed weights, germination, first germination count, germination speed index, accelerated aging, seedling length, dry matter transfer, and emergence sand test were evaluated. IST did not affect the moisture content and weight of a thousand seeds. However, storage in a controlled environment provided a greater reduction in moisture content and a smaller reduction in the weight of a thousand seeds. IST negatively affected germination, first germination count, germination speed index, and accelerated aging test. IST negatively affected plant length, except in the treatment where only insecticide was applied. The dry mass transfer was negatively affected but with less intensity in seeds subjected only to the application of insecticide or fungicide and the combination of insecticide, fungicide, and polymer. Seed emergence in the sand was negatively affected by all treatments. Regardless of the treatments, all responses evaluated showed a reduction throughout storage, with greater intensity in seeds stored in the natural environment.

The Impact of ZnO and Fe2O3 Nanoparticles on Sunflower Seed Germination, Phenolic Content and Antiglycation Potential.

The enhancement of seed germination by using nanoparticles (NPs) holds the potential to elicit the synthesis of more desired compounds with important biomedical applications, such as preventing protein glycation, which occurs in diabetes. Here, we used 7 nm and 100 nm ZnO and 4.5 nm and 16.7 nm Fe2O3 NPs to treat sunflower seeds. We evaluated the effects on germination, total phenolic content, and the anti-glycation potential of extracted polyphenols. Sunflower seeds were allowed to germinate in vitro after soaking in NP solutions of different concentrations. Polyphenols were extracted, dosed, and used in serum albumin glycation experiments. The germination speed of seeds was significantly increased by the 100 nm ZnO NPs and significantly decreased by the 4.5 nm Fe2O3 NPs. The total phenolic content (TPC) of seeds was influenced by the type of NP, as ZnO NPs enhanced TPC, and the size of the NPs, as smaller NPs led to improved parameters. The polyphenols extracted from seeds inhibited protein glycation, especially those extracted from seeds treated with 7 nm ZnO. The usage of NPs impacted the germination speed and total polyphenol content of sunflower seeds, highlighting the importance of NP type and size in the germination process.

Effects of a S-metolachlor based herbicide on two plant models: Zea mays L. and Lactuca sativa L

ABSTRACT Corn is the second most cultivated crop in Brazil, the number-one country in pesticide consumption. Chemical control of weeds is performed using herbicides such as S-metolachlor with pre- and post-emergence action and thus the toxicity of herbicides constitutes a matter of great concern. The present investigation aimed to examine the effects of an S-metolachlor-based herbicide on Lactuca sativa L. (lettuce) and Zea mays L. (maize) utilizing various bioassays. The test solutions were prepared from commercial products containing the active ingredient. Seeds from the plant models were exposed in petri dishes and maintained under biochemical oxygen demand (BOD) at 24°C. Distilled water was negative and aluminium positive control. Macroscopic analyses (germination and growth) were conducted for both plant species, and microscopic analysis (cell cycle and chromosomal alterations) were performed for L. sativa root tip cells. Detrimental interference of S-metolachlor-based herbicide was noted with lettuce for all parameters tested reducing plant germination by over 50% and the germination speed by over 45% and showing a significant decrease in mitotic index, from 16.25% to 9,28% even on the lowest concentration tested. In maize, there was no significant interference in plant germination; however, speed of germination was significantly hampered, reaching a 51.22% reduction for the highest concentration tested. Data demonstrated that the herbicide was toxic as evidenced by its phyto- and cytotoxicity in L. sativa L. and Z. mays L.

Seed functional traits as predictors of seedling establishment success in Brazilian tropical forest restoration

AbstractSeveral ecological filters in deforested and degraded areas reduce seedling emergence and establishment and hinder ecological restoration by direct seeding. Understanding whether functional traits are related to a species' capacity to overcome these filters and predict their field performance might improve the success of direct seeding techniques for ecological restoration. We assessed eight seed functional traits of tropical tree species, seeking those that best explained their establishment success in direct seeding restoration projects. We analyzed a dataset from 52 studies that tested direct seeding techniques with tree species in Brazil. From each study, we collected the mean establishment percentage for all tree species. Seed mass, cotyledon function, and germination speed were the only functional traits that significantly affected the species establishment percentage in direct seeding restoration projects. Species with larger seeds, storage cotyledons, and faster germination had higher establishment percentages. Choosing species with these functional traits for seed mixes will provide higher establishment percentages and, consequently, improve restoration success by direct seeding techniques.

Is pearl millet (Pennisetum glaucum) a good plant species for ecotoxicological tests?

Various plant species can be selected for environmental testing, including pearl millet (Pennisetum glaucum (L.) R. Br), a globally significant cereal crop. This study aims to assess millet's suitability as a species for ecotoxicological tests, examining (1) germination and initial development dynamics, (2) the minimum seed quantity for reliable sampling, (3) optimal experimental design with replication numbers, (4) suitability of positive control, and (5) the effectiveness of the protocol in evaluating toxic effects of environmental pollutants. Millet exhibited rapid and uniform germination as well as consistent initial seedling development. To establish the minimum number of seeds required for reliable experimentation, germination, and seedling growth were compared across plots containing 10, 25, and 50 seeds. Consequently, 10 seeds per plot were chosen for subsequent experiments to reduce labor and costs while maintaining reliability. To validate the selected experimental design, and to establish a positive control for assays, aluminum was used as a toxic element at concentrations ranging from 10-2 to 10-6 M. While aluminum did not affect the final percentage of germinated seeds, it did exhibit an impact on the Germination Speed Index (GSI). Significant differences in root and aerial growth, and with fresh weight, were observed. The 10-3M concentration was chosen as the positive control as the 10-2 concentration showed extreme toxicity. To assess the applicability of the established protocol in determining the toxic effects of environmental pollutants, millet roots were exposed to the toxic agents atrazine, cadmium, methyl methane sulfonate (MMS), and Spent pot liner (SPL). Millet demonstrated sensitivity and efficiency in response to these tests. In conclusion, millet proves to be an effective species for the toxicological risk assessment of environmental pollutants.

Dry matter distribution, yield and seed quality of soybean (Glycine max) genotypes as affected by water stress

Water stress is a major constraint for crop productivity and culturing right cultivars may produce a considerable yield under such stressful condition. An experiment was conducted inside a vinyl house to evaluate the effect of water stress on dry matter distribution, yield, and seed quality of eight soybean genotypes, viz. G00006, BD2336, AGS383, PK472, BCS-1, NCS-1, BU Soybean-1 and BARI Soybean-6. They were grown in pots and subjected to water stress (20% of field capacity, FC) and control (80% of FC). The water stress reduced plant height, leaf number, leaf, stem, and root dry matter by 23, 45, 46, 45 and 19%, respectively, across the genotypes. Under water stress, the soybean genotypes G00006, BCS-1, NCS-1 and BARI Soybean-6 beard only 6 to 30% pod and 5 to 34% seed compared to the control condition. The results further indicated that yield of BD2336 and AGS383 were less affected by the stress than those of other genotypes. Interestingly, water stress exerted positive effect on seed germination, viability, speed of germination and vigor index in BD2336 and AGS383, respectively. Nitrogen and seed protein content were found the highest in BCS-1 under control (9.82 and 58.42% respectively) followed by AGS383. Phosphorus content in seed also reduced by the stress in the tested genotypes, except BD2336 (0.29% in control and 0.96% in water stress) and BARI Soybean-6. Potassium content in seed was reduced by the stress in the tested genotypes, except G00006 and BARI Soybean-6. Based on the findings related to water stress effects on yield and seed quality, particularly seed protein of the tested eight soybean genotypes, it was concluded that genotypes AGS383 and BD2336 might be considered for field trial under water deficit condition.

Breaking the Dormancy of Some Varieties of Papaya (Carica Papaya L.) Soaking KNO3 Solution

Dormancy is a state of stopping growth experienced by plants. This research aimed to determine the concentration of KNO3 solution in breaking dormancy in several papaya varieties. The research method used in this research was a completely randomized design (RAL) Factorial with 2 treatment factors. The first factor is variety (V), consisting of V1 = Callina, V2 = California, and V3 = Local. The second factor is the concentration of the KNO3 (K) solution consisting of: K0 = Control, K1 = 10%, K2 = 20% and K3 = 30%. The variables observed were growth synchrony and germination speed index. Data analysis uses analysis of variance. The variables observed were growth synchrony and germination speed index. An analysis of variance was used to test the effect of treatment. To test mean differences between treatments, honest significant difference tests, regression, and correlation were carried out at a test level of 5%. The research results showed that seed variety had a significant effect on growth synchrony and germination speed index. The concentration of KNO3 solution has a significant effect on growth synchrony and germination speed index. The interaction between the variety and concentration of KNO3 solution had no significant effect on all observed parameters.

Marigold Seed Pelleting with Plant Nutrients on Germination, Growth, Storage and Flower Yields

Background: Seed pelleting, a method involving seed coatings to enlarge seed size, is advantageous for marigold seeds because it improves water utilization efficiency during germination. Furthermore, the incorporation of vital plant nutrients into seeds fosters germination and growth, facilitating accelerated growth and increased yield in marigold plants. Methods: Marigold seeds were pelleted with NH4NO3 at rates of 0.048, 0.096 and 0.192 g, NaH2PO4.H2O at rates of 0.064, 0.128 and 0.256 g and KCl at rates of 0.013, 0.026 and 0.052 g. Growth was determined after incubation in 4 × 6 inch bags for 56 days. Storage longevity was determined after incubation in aluminum foil bags under controlled (15°C, 50% RH) and ambient conditions (27±2°C, 70% RH) for 6 months. Result: Seeds pelleted with NH4NO3, NaH2PO4.H2O and KCl demonstrated superior germination rates and germination speed. NaH2PO4.H2O pelleting led to increased shoot dry weight. The use of 0.256 g NaH2PO4.H2O resulted in the highest germination rate over 6 months. Pelleting with all three nutrients resulted in higher plants, particularly 0.256 g NaH2PO4.H2O, which led to more flowers per plant. Therefore, pelleting seeds with 0.256 g NaH2PO4.H2O is recommended to optimize the quality of marigold seeds.

Seed physiological traits and environmental factors influence seedling establishment of vegetable soybean (Glycine max L.).

Edamame (Glycine max (L.) Merr.), a specialty soybean prized for its nutritional value and taste, has witnessed a surge in demand within the U.S. However, subpar seedling stands have hindered its production potential, necessitating increased inputs for farmers. This study aims to uncover potential physiological factors contributing to low seedling emergence in edamame. We conducted comprehensive assessments on thirteen prominent edamame genotypes alongside two food-grade and two grain-type soybean genotypes, focusing on germination and emergence speed in both laboratory and field settings. Additionally, we employed single electrical conductivity tests and identified and quantified seed leachate components to distinguish among soybean types. Furthermore, using a LabField™ simulation table, we examined seed emergence across a wide soil temperature range (5°C to 45°C) for edamame and other soybean types. All seeds were produced under the same environmental conditions, harvested in Fall 2020, and stored under uniform conditions to minimize quality variations. Our findings revealed minimal divergence in emergence percentages among the seventeen genotypes, with over 95% germination and emergence in laboratory conditions and over 70% emergence in the field. Nonetheless, edamame genotypes typically exhibited slower germination speeds and higher leachate exudates containing higher soluble sugars and amino acids. Seed size did not significantly impact total emergence but was negatively correlated with germination and emergence speed, although this effect could be mitigated under complex field conditions. Furthermore, this study proposed differences that distinguish edamame from other soybean types regarding ideal and base temperatures, as well as thermal time. The finds offer valuable insights into edamame establishment, potentially paving the way for supporting local edamame production in the U.S.

Mother-reliant or self-reliant: the germination strategy of seeds in a species-rich alpine meadow is associated with the existence of pericarps.

Some plants germinate their seeds enclosed by a pericarp, whereas others lack the outer packaging. As a maternal tissue, the pericarp might impart seeds with different germination strategies. Plants in a community with different flowering times might separately disperse and germinate their seeds; therefore, flowering time can be considered as one manifestation of maternal effects on the offspring. The mass of the seed is another important factor influencing germination and represents the intrinsic resource of the seed that supports germination. Using seeds from a species-rich alpine meadow located in the Hengduan Mountains of China, a global biodiversity hotspot, we aimed to illustrate whether and how the type of seed (with or without a pericarp) modulates the interaction of flowering time and seed mass with germination. Seeds were germinated in generally favourable conditions, and the speed of germination [estimated by mean germination time (MGT)] was calculated. We quantified the maternal conditions by separation of flowering time for 67 species in the meadow, of which 31 produced seeds with pericarps and 36 yielded seeds without pericarps. We also weighed 100 seeds of each species to assess their mass. The MGT varied between the two types of seeds. For seeds with pericarps, MGT was associated with flowering time but not with seed mass. Plants with earlier flowering times in the meadow exhibited more rapid seed germination. For seeds without a pericarp, the MGT depended on seed mass, with smaller seeds germinating more rapidly than larger seeds. The distinct responses of germination to flowering time and seed mass observed in seeds with and without a pericarp suggest that germination strategies might be mother-reliant for seeds protected by pericarps but self-reliant for those without such protection. This new finding improves our understanding of seed germination by integrating ecologically mediated maternal conditions and inherent genetic properties.

BIOESTIMULANTE Y TIEMPOS DE IMBIBICIÓN SOBRE LA GERMINACIÓN DE SEMILLAS DE MARACUYÁ (Passiflora edulis S., Passifloraceae

<p><strong>Background: </strong>Passion fruit (<em>Passiflora edulis</em> S.) is a tropical fruit of socio-economic interest in Peru, used as an ingredient in the food industry, esthetics and medicine. However, this crop has a low germination power, so it is necessary to carry out pre-germination treatments to increase the germination percentage and the initial development of the seedlings. <strong>Objective:</strong> to evaluate the effect of different biostimulants at 12 h and 24 h of hydration on passion fruit seed germination. <strong>Methodology: </strong>passion fruit seeds of large and round fruits, free of pathogens, were collected. The aryl-free seeds were treated: T0 (distilled water), T1 (sulfur 25 g/10 L), T2 (sulfur 50 g/10 L), T3 (biol 3%), T4 (biol 5%), T5 (cinnamon 25%), T6 (cinnamon 50%), T7 (buttermilk 25%) and T8 (buttermilk 50%) for 12 h and 24 h imbibition. The variables evaluated were: water absorbed, imbibition rate (IR), germination percentage (GP), germination speed (GS), germination index (GI), first day of germination (FDG). <strong>Results:</strong> The highest water absorption occurred in the first 9 hours. Treatments T4, T5 and T6 significantly improved the GP, GS, GI, FDG at 12 h imbibition. However, at 24 h imbibition these values decreased. GP presented a high positive correlation with GS and GI. Likewise, GS presented a high negative correlation with FDG. <strong>Implications: </strong>The use of biostimulants increases germination. <strong>Conclusions:</strong> Cinnamon extract at 25% and 50%, and biol 5% at 12 h of imbibition have significantly improved the variables evaluated compared to imbibition at 24 h. The positive correlation between the variables indicates a direct relationship between them.</p>

Application of chitosan nanopriming on plant growth and secondary metabolites of Pancratium maritimum L

BackgroundNanotechnology has demonstrated its vital significance in all aspects of daily life. Our research was conducted to estimate the potential of primed seed with chitosan nanoparticles in seed growth and yield by inducing plant secondary metabolism of Pancratium maritimum L. one of the important medicinal plants. Petri dish and pot experiments were carried out. Seeds of Pancratium maritimum L. were soaked in Nano solution (0.1, 0.5, 1 mg/ ml) for 4, 8, 12 h. Germination parameters (germination percentage, germination velocity, speed of germination, germination energy, germination index, mean germination time, seedling shoot and root length, shoot root ratio, seedling vigor index, plant biomass and water content), alkaloids and antioxidant activity of Pancratium maritimum L. were recorded and compared between coated and uncoated seeds.ResultsOur results exhibited that chitosan nanopriming had a positive effect on some growth parameters, while it fluctuated on others. However, the data showed that most germination parameters were significantly affected in coated seeds compared to uncoated seeds. GC-MS analysis of Pancratium maritimum L. with different nanopriming treatments showed that the quantity of alkaloids decreased, but the amount of pancratistatin, lycorine and antioxidant content increased compared with the control.ConclusionsApplying chitosan nanoparticles in priming seeds might be a simple and effective way to improve the quantity of secondary metabolites of Pancratium maritimum L. valuable medicinal plant.

Discovery of seed germinating fungi (Mycetinis scorodonius) from Gastrodia elata Bl. f. glauca S. chow in Changbai Mountain and examination of their germination ability

Multi-generational asexual reproduction of Gastrodia elata Bl. will cause seedling species degeneration. Sexual reproduction of Gastrodia elata Bl. seed is an effective method to solve the problem of degeneration. The development of Gastrodia elata Bl. seeds cannot be separated from the germination fungus. However, there are few strains of germination fungus in production, and there is also the problem of species degradation in application for many years. It is very important for the sexual reproduction of Gastrodia elata Bl. to isolate more new strains of excellent germination fungus from the origin. This study used the Gastrodia elata Bl. f. glauca S. chow seeds germination vegetative propagation corms capture method to isolate its symbiotic germination fungus, and comprehensively identified the species of germination fungus by colony morphology, ITS, sporocarps regeneration and germination function, and compared the growth characteristics and germination ability with other germination fungus (Mycena purpureofusca, Mycena dendrobii and Mycena osmundicola). The germination fungus was isolated from the vegetative propagation corms of Gastrodia elata Bl. f. glauca S. chow seeds and named GYGL-1. After comprehensive identification, GYGL-1 was Mycetinis scorodonius. Compared with other germination fungus, GYGL-1 has fast germination speed, vigorous growth, and high germination ability for Gastrodia elata Bl. f. glauca S. chow seeds. Innovated the isolation method of Gastrodia elata Bl. seeds germination fungus, obtained the regenerated sporocarps of the germination fungus, and discovered that Mycetinis scorodonius has a new function of germinating Gastrodia elata Bl. f. glauca S. chow seeds, enriching the resource library of Gastrodia elata Bl. germination fungus.

Influence of Gibberellic Acid Growth Regulator on Sunflower Seed Emergence Indices and Seedling Characters under Salinity Stress

Salinity stress is one of factors limiting seed germination and seedling development in sunflower at New halfa scheme in East of Sudan. The objective of this study was evaluation germination indices and seedling parameters of sunflower seed under salinity stress with varying concentrations of synthetic growth regulator namely GA3. From 16-30 November 2019 a laboratory experiment (in Petri dishes) of randomized complete blocks design (RCBD) with three replicates was carried out. Treatments were consisted of (four levels of GA3 were 0, 50,75 and 100 p.pm) designated as G0,G50,G75 and G100,respectively, under five levels of salinity (0, 0.5, 1.0, 1.5 and 2%) designated as Na0, Na1.0 ,Na1.5 and Na2.0. This study examined Emergence indices: Seed Emergence percentage EP%, Mean emergence time (MET), Mean daily germination (MDG), Energy of germination was( EE), Emergence index(EI), Daily germination speed (DGS), Emergence coefficient of uniformity (ECU), vigour index of seed(VIS) and seedling characters also were determined. The results indicated that application of GA3 at 50 p.pm resulted in high EP, MDG, MET and SVI as compared with other related treatments. Also application of (G75 and G100) resulted in higher values of DGS, ECU and EI. Application of high levels of salinity treatments resulted in decreasing of all germination indices in this study. The interaction treatment ( G50xNa0) on MDG had significant differences in this study. In condition, GA3 increasing up germination indices and promoting growth and compromised seedling vigor while salinity hindered seed emergence and seedling characters of sunflower.