Rapeseed Germination Research Articles

Soil Moisture Detection and Linear Deceleration Control Strategy Enhancing Trenching Depth Precision and Stability for Rapeseed Sowing

Sowing depth significantly affects the germination of rapeseed, and different soil moisture conditions require corresponding sowing depths. However, most current trenching devices do not account for soil moisture content, and commonly used hydraulic or constant-force trenching equipment also exhibits deficiencies in stability and consistency. To address these challenges, this study developed an automatic depth adjustment control system based on soil moisture content. A soil moisture detection device and an innovative sliding mechanism that maintained the soil moisture sensor in a relatively stationary position relative to the soil during seeder movement were introduced. An automatic sowing depth adjustment device was designed to modulate the sowing depth. A control strategy that incorporated the Kalman filtering algorithm and linear deceleration equations was conducted. At an observation noise covariance matrix (Q/R) of 0.001, a deceleration range of 40 mm and a minimum speed of 10, the control system exhibited minimal overshoot (approximately 4%) and steady-state error (approximately 3.2 mm). It effectively adjusted the trenching depth while operating at speeds ranging from 2 to 3.6 km/h, successfully adapting to variations in soil topography. The system performance tests revealed that the control system adjustment time (ts) was 534 ms and the steady-state error remained within 1 mm. Under three different soil moisture content conditions, the sowing depth qualification rate and stability coefficients consistently surpassed 90% and 80%, respectively. This research offers a sowing depth adjustment control system based on soil moisture content, contributing to more precise depth regulation for rapeseed sowing.

Conversion of lipids into carbohydrates rescues energy insufficiency in rapeseed germination under waterlogging stress.

Waterlogging stress, particularly during seed germination, significantly affects plant growth and development. However, the physiological and molecular mechanisms underlying waterlogging stress responses during rapeseed germination remain unclear. In this study, two rapeseed cultivars, Xiangzayou518 (waterlogging-sensitive) and Dadi199 (waterlogging-tolerant), were used to explore the physiological mechanisms underlying rapeseed response to waterlogging stress during germination. Our results showed that waterlogging significantly decreased the emergence percentage and seedling growth rate. During the radicle elongation period (from 48 to 96 h post-germination), the most sensitive period to waterlogging during germination, sugar content, and glycolysis efficiency were significantly decreased, but anaerobic fermentation was enhanced. In tolerant cultivars, when the energy supply was insufficient, the conversion efficiency of lipids into sugar increased, and the activities of isocitrate lyase, malate synthase, and fructose-1, 6-diphosphatase were enhanced by 11.63, 19.06, and 20.37%, respectively, at 72 h post-germination under waterlogging stress. Transcriptome data showed that the differentially expressed genes were significantly enriched in glucose and lipid metabolism pathways when comparing waterlogged stress and normal conditions. These results indicate that waterlogging affects seed germination in rapeseed by inhibiting carbohydrate metabolism, and the conversion capacity of lipids into sugar under waterlogging stress was stronger in tolerant cultivars than in sensitive cultivars, thus rescuing the insufficient energy supply in seed germination and seedling growth. This study reveals the physiological mechanism of rapeseed response to waterlogging stress during seed germination and provides a valuable reference for improving waterlogging tolerance.

Engineering Rapeseed Germination and Root Growth with Mechanical Strength of Polysaccharide Hydrogel.

Plant roots are highly sensitive to physical stress in the soil, with appropriate mechanical impedance promoting root elongation and lateral root growth. However, few studies have quantitatively explored the relationship between the mechanical impedance of the growth medium and the phenotypes of plant roots. In this study, we used a tensile machine equipped with a self-made steel needle mimicking the root tip to measure the force needed to penetrate the hydrogel medium (agar, low acyl gellan gum, and κ-carrageenan), providing insights into the force required for the rapeseed root tip to enter the medium following germination. These findings indicate that root penetration length is inversely associated with the mechanical strength of the growth medium, with variations observed in the root system adaptability across different substrates. Specifically, when the gel puncture resistance of the culture medium without adding MS reached approximately 18.4 mN, root penetration and growth were significantly hindered. With the addition of 1/2 MS medium, the polysaccharide concentration is 1.0 wt %, which is more suitable for cultivating rapeseed. This research not only offers a method for quantifying root phenotypes and medium mechanical impedance but also presents an approach for plant growth regulation and crop breeding.

Nanopriming with selenium doped carbon dots improved rapeseed germination and seedling salt tolerance

Soil salinity is a big environmental issue affecting crop production. Although seed nanopriming has been widely used to improve seed germination and seedling growth under salinity, our knowledge about the underlying mechanisms is still insufficient. Herein, we newly synthesized selenium-doped carbon dots nanoparticles coated with poly acrylic acid (poly acrylic acid coated selenium doped carbon dots, PAA@Se-CDs) and used it to prime seeds of rapeseeds. The TEM (transmission electron microscope) size and zeta potential of PAA@Se-CDs are 3.8 ± 0.2 nm and −30 mV, respectively. After 8 h priming, the PAA@Se-CDs nanoparticles were detected in the seed compartments (seed coat, cotyledon, and radicle), while no such signals were detected in the NNP (no nanoparticle control) group (SeO2 was used as the NNP). Nanopriming with PAA@Se-CDs nanoparticles increased rapeseeds germination (20%) and seedling fresh weight (161%) under saline conditions compared to NNP control. PAA@Se-CDs nanopriming significantly enhanced endo-β-mannanase activities (255% increase, 21.55 µmol h−1 g−1 vs. 6.06 µmol h−1 g−1, at DAS 1 (DAS, days after sowing)), total soluble sugar (33.63 mg g−1 FW (fresh weight) vs. 20.23 mg g−1 FW) and protein contents (1.96 µg g−1 FW vs. 1.0 µg g−1 FW) to support the growth of germinating seedlings of rapeseeds under salt stress, in comparison with NNP control. The respiration rate and ATP content were increased by 76% and 607%, respectively. The oxidative damage of salinity due to the over-accumulation of reactive oxygen species (ROS) was alleviated by PAA@Se-CDs nanopriming by increasing the antioxidant enzyme activities (SOD (superoxide dismutase), POD (peroxidase), and CAT (catalase)). Another mechanism behind PAA@Se-CDs nanopriming improving rapeseeds salt tolerance at seedling stage was reducing sodium (Na+) accumulation and improving potassium (K+) retention, hence increasing the K+/Na+ ratio under saline conditions. Overall, our results not only showed that seed nanopriming with PAA@Se-CDs could be a good approach to improve salt tolerance, but also add more knowledge to the mechanism behind nanopriming-improved plant salt tolerance at germination and early seedling growth stage.

Morpho-Physiochemical Indices and Transcriptome Analysis Reveal the Role of Glucosinolate and Erucic Acid in Response to Drought Stress during Seed Germination of Rapeseed.

The global expansion of rapeseed seed quality has been focused on maintaining glucosinolate (GSL) and erucic acid (EA) contents. However, the influence of seed GSL and EA contents on the germination process under drought stress remains poorly understood. Herein, 114 rapeseed accessions were divided into four groups based on GSL and EA contents to investigate their performance during seed imbibition under drought stress. Our results revealed significant variations in seed germination-related traits, particularly with higher GSL and EA, which exhibited higher germination % (G%) and lower mean germination time (MGT) under drought stress conditions. Moreover, osmoregulation, enzymatic system and hormonal regulation were improved in high GSL and high EA (HGHE) versus low GSL and low EA (LGLE) seeds, indicating the essential protective role of GSL and EA during the germination process in response to drought stress. The transcriptional regulation mechanism for coordinating GSL-EA-related pathways in response to drought stress during seed imbibition was found to involve the differential expression of sugar metabolism-, antioxidant-, and hormone-related genes with higher enrichment in HGHE compared to LGLE seeds. GO enrichment analysis showed higher variations in transcription regulator activity and DNA-binding transcription factors, as well as ATP and microtubule motor activity in GSL-EA-related pathways. Furthermore, KEGG analysis identified cellular processes, environmental information processing, and metabolism categories, with varied gene participation between GSL, EA and GSL-EA-related pathways. For further clarification, QY7 (LGLE) seeds were primed with different concentrations of GSL and EA under drought stress conditions. The results showed that 200 μmol/L of GSL and 400 μmol/L of EA significantly improved G%, MGT, and seedling fresh weight, besides regulating stress and fatty acid responsive genes during the seed germination process under drought stress conditions. Conclusively, exogenous application of GSL and EA is considered a promising method for enhancing the drought tolerance of LGLE seeds. Furthermore, the current investigation could provide a theoretical basis of GSL and EA roles and their underlying mechanisms in stress tolerance during the germination process.

Проращивание семян рапса при непрерывном светодиодном освещении в режимах низкоэнергетических потоков фотонов монохроматического излучения

In order to study the effect of low-energy monochrome radiation on the germination of rapeseed of the new variety Antares, the authors used light-emitting diodes of blue (440 nm), green (525 nm), red (660 nm) and far red (730 nm) light, as well as those of UV-A radiation (380 nm) with an intensity of 0.367–6.904 µmol/m2* s on different spectra. Different types of monochrome radiation affected the germination of seeds and the growth of sprouts in different ways. According to the indicators of germination energy, seed germination, weight of 100 sprouts, and yield of green mass, the best results were obtained when germinating using the green light. Based on experimental data, the patent was issued for the invention “The activation method of rapeseed germination under monospectral illumination”. Irradiation of seeds with the monochrome far-red light significantly reduced the germination ability and yield of sprouts.

Integrated analysis of transcriptome and metabolome reveals insights for low-temperature germination in hybrid rapeseeds (Brassica napus L.)

Rapeseed (Brassica napus L.) is an important oil-producing crop in China. However, cold stress in winter can adversely affect rapeseed germination and subsequently result in poor seed yield at the mature stage. Studies of differences in the transcriptional and metabolic levels of rapeseed under cold stress can improve our understanding of low-temperature germination (LTG). The current study aimed to identify the cold stress-responsive genes, metabolites, and metabolic pathways based on a combined transcriptome and metabolome analysis to understand the difference of LTG and tolerance mechanisms in the cold-tolerant (Yueyou1301, YY1301) and cold-normal (Fengyou737, FY737) rapeseed varieties. Compared to FY737, YY1301 had a higher germination rate, indole acetic acid (IAA) and gibberellic acid (GA)/(abscisic acid) ABA levels at 7.5 °C. A total of 951 differentially expressed genes (DEGs) and 86 differentially accumulated metabolites (DAMs) were identified in two rapeseed varieties. Conjoint analysis revealed 12 DAMs and 5 DEGs that were strongly correlated in inducing rapeseed LTG, which were mainly related to carbohydrate and amino acid metabolism, specifically the pathway of glutathione metabolism and starch and sucrose metabolism. These results suggest that the DAMs and DEGs involved in crucial biological pathways may regulate the LTG of rapeseed. It increases the understanding of the molecular mechanisms underlying the adaptation of rapeseed to LTG.

Application of microcosms models for ecologically based selection of biofuel crops

One of the possible ways to solve the problem of shortage and rise in price of energy resources can be the development of the biofuel industry in Ukraine, the basis of which is the scientifically reasoned selection of plants (resistant to regional pollutants), taking into account the specifics of the regions. The article summarizes scientific data on the peculiarities of germination of some energy crops, considers the possibility of using microcosms for the reasoned selection of biofuel crops. The study was carried out in microcosmic models, seed germination and biometric parameters of seedlings Brassica napus L. and Zea mays L. were studied. Based on the application of the method of microcosmic models, the resistance of B. napus to lead acetate and sensitivity to the fungicide «Raxil ultra» were proven. To protect rapeseed from pests, systemic preparations with the active ingredients dimethoate, gamma-cyhalothrin, alphacypermethrin, thiamethoxam, fludioxanil, metalaxyl, imidocloprid, etc., are used. The insecticide is used for seed treatment before sowing (in combination with a fungicide), as well as for the treatment of crops when the economic threshold of harmfulness is reached. It was found that pre-sowing treatment of rapeseed should be carried out with lower concentrations of fungicide compared to Z. mays seeds. It was found that the Galitsky variety is characterized by the highest laboratory germination of seeds and the best morpho-physiological parameters of seedlings. It was found that the fungicide «Raksil ultra» in high concentration inhibits seed germination (at the late stages of germination), stem growth, cheese and dry biomass of corn seedlings, at a low concentration an increase in the dry weight of the aerial part was detected. Laboratory germination of rapeseed was inhibited at all stages of germination. None of the studied concentrations of the fungicide affect the raw and dry biomass of rapeseed seedlings, all the studied concentrations of the fungicide inhibit the root growth of rapeseed seedlings, and the largest inhibit the growth of the stem.

A novel type of sprout food development: Effects of germination on phytic acid, glucosinolates, and lipid profiles in rapeseed

Germination, a new processing technology, is widely used to improve the nutritional value of plant seeds. Cereals and legumes sprouts are widely popular around the world. In this study, a novel form of sprouts-based rapeseed was produced. Germination promoted the degradation of phytic acid and glucosinolates (GLs) in rapeseed. On the fourth day of germination, the degradation rate of phytic acid in rapeseed was nearly 80%, and the degradation rate of GLs reached more than 90%. The changes in lipid species and content by sprouting were assessed. The crude fat content was significantly reduced during rapeseed germination. Using non-targeted lipidomics, three hundred eighty-nine lipid molecules in 4 categories and 17 subclasses were identified in rapeseed/rapeseed sprouts. Glycerides were most abundant, followed by fatty acids, glycerophospholipids, and sphingolipids. Germination affected not only the lipid content but also its composition. Some functional lipids (such as FAHFAs) were generated and enriched during germination. The content of monounsaturated fatty acids first raised and then decreased during germination. The finding will serve as a theoretical reference for developing novel sprouts based on rapeseed, furnish primary data concerning the nutritional content of rapeseed sprouts, and provide fresh ideas for rapeseed resource exploitation.

An improved multi-objective brainstorming algorithm with the application of rapeseed germination characteristics optimization

As one of the most important raw materials for daily edible oil, the annual production and quality of oilseed rape have a great impact on the daily lives of people and the development of the country. The germination quality of rapeseed directly affects the future yield and quality of oilseed rape, and seed germination vigor (SGV) and chlorophyll content (CC) are important factors affecting the quality of rapeseed germination. It is very necessary to make the rapeseeds have optimal germination characteristics and seed germination vigor at the early stage of germination by controlling the combination of each decision variable at the segmented harvest. The two objectives are coupled with each other to form a complex multi-objective optimization problem, which is important in the current research field of the rapeseed problem. To solve this problem, a modified brainstorming optimization algorithm based on OPTICS clustering, named MOPCBSO, is proposed in this study. The algorithm uses optics clustering as a new clustering method, chaotic mutation as a new updating method for individuals, and introduces an elite strategy and a tracking strategy. 19 benchmark test functions and nonparametric tests are adopted to examine the performance of the MOPCBSO compared with other optimization algorithms. The results show that the proposed algorithm has significant advantages over other algorithms in terms of convergence and comprehensiveness, demonstrating the effectiveness of the algorithm improvement. To optimize rapeseed germination characteristics, mathematical models are built, in which, cut-down time, after-ripening time and stubble height are selected as decision variables, while chlorophyll concentration and seed germination vigor in seeds as the target characteristics. The data of oliseed rape field trials were collected from 2020 to 2021 in Huanggang City, Hubei Province. Finally, the optimal solution for the germination characteristics of rapeseeds was obtained by the MOPCBSO, which provided an effective basis for the subsequent rapeseed production experiments.

Changes in Oil Quality and Peroxidase Activity during Germination of Rape Seeds and Mustard Seeds

Vegetable oils are selected by consumers based on the presence of multiple bioactive substances, including polyunsaturated fatty acids, sterols, and tocopherols. Another important factor in oils is their quality. This research involved analyzing the oils quality and quantity of bioactive substances obtained from rape seeds and white mustard seeds that underwent germination. The quality of the oils was compared by determining the acid and peroxide values. Germination lowered the peroxide value by 86.3% and 71.4% for rapeseed oil and mustard oil, respectively. This was due to the germination step of the seed use oxygen, which was the substrate for lipid peroxidation. The activity of peroxidase increased by 95% for rapeseed oil and 94% for mustard oil during germination. An increase in the amount of polyunsaturated fatty acids in mustard oil also was noted during germination.

The Effects of Temperature and Water on the Seed Germination and Seedling Development of Rapeseed (Brassica napus L.).

The seed germination and seedling growth of rapeseed are crucial stages in plant life, especially when facing abiotic stresses. In the present work, the effects of water and temperature on seed germination and seedling growth were investigated in a rapeseed crop (Brassica napus L.). The plants were examined under different temperature levels (5 °C, 10 °C, 15 °C, 20 °C, 25 °C, 30 °C, and 35 °C) and water levels (twenty-nine levels based on either one-milliliter intervals or as a percentage of the thousand-kernel weight (TKW)). Moreover, planting densities and antifungal application techniques were investigated in the study. The findings demonstrated substantial variations between all the growth parameters investigated at all the tested temperatures, and 20 °C was considered the optimum within a broad range of 15-25 °C. Water availability plays a significant role in germination, which can be initiated at 0.65 mL, corresponding to 500% of the TKW. The method of TKW is a more accurate aspect of water application because of the consideration of the seed weight and size. The optimal water range for the accumulation of dry weight, 3.85-5.9 mL (2900-4400% of TKW), was greater than that required for seedling growth, 1.45-3.05 mL (1100-2300% of TKW). Twenty to twenty-five seeds per 9 cm Petri dish exhibited the most outstanding values compared to the others, which provides an advantage in breeding programs, especially when there are seed limitations. Seed priming is a more effective antifungal application strategy. These data can be incorporated into future rapeseed germination in vitro studies, breeding programs, and sowing date predictions.

Mitigation of the salinity stress in rapeseed (Brassica napus L.) productivity by exogenous applications of bio-selenium nanoparticles during the early seedling stage

In recent years, much attention has been directed toward using nanoparticles (NPs) as one of the most effective strategies to improve plant growth, especially under salt stress conditions. Further research has been conducted to develop NPs using various chemical ways; accordingly, knowledge about the beneficial effect of bioSeNPs in rapeseed is obscure. Selenium (Se) is a vital micronutrient with a series of physiological and antioxidative properties. Seed priming is emerging as a low-cost, efficient, and environment-friendly seed treatment in nanotechnology. The current study was carried out to examine the promising effects of nanopriming via bioSeNPs on the expression level of aquaporin genes, seed microstructure, seed germination, growth traits, physiochemical attributes, and minerals uptake of two rapeseed cultivars under salinity stress conditions. Our investigation monitored the positive effects of bioSeNPs on the expression level of aquaporin genes (BnPIP1-1 and BnPIP2-1) and water uptake during the seed imbibition (4 and 8 h of priming), which indicated higher imbibition potential and germination promotion with bioSeNPs application (most effective at 150 μmol/L). The total performance index was significantly enhanced with nano-treatments in rapeseed seedlings. Collectively, nano-application improved seed microstructure, seed germination, and photosynthetic efficiency directly correlated with higher seedlings biomass, especially with a higher concentration of bioSeNPs. The enhancement in α-amylase and free amino acid contents in nanoprimed seeds resulted in rapid seed germination. Moreover, bioSeNPs increased the osmotic adjustment and enhanced the efficiency of the plant's defense system by improving the activity of enzymatic and non-enzymatic antioxidants, thus enhancing ROS scavenging under salt stress. The obtained results may indicate the strengthening of seed vigor, improving seedling growth and physiochemical attributes via bioSeNPs. Our findings displayed that bioSeNPs modulated the Na+ and K+ uptake, which improved the rapeseed growth and showed a close relationship with the low contents of toxic Na+ ion; thus, it prevented oxidative damage due to salt stress. This comprehensive data can add more knowledge to understand the mechanisms behind plant-bioSeNPs interaction and provide physiological evidence for the beneficial roles of nanopriming using bioSeNPs on rapeseed germination and seedling development under salinity stress conditions. Such studies can be used to develop simple prepackaged nano primer products, which can be used before sowing to boost seed germination and crop productivity under stress conditions.

Single and joint toxicity of polymethyl methacrylate microplastics and As (V) on rapeseed (Brassia campestris L.)

Most microplastics and arsenic (As) have been released into farmland via industrial and agricultural activities, posing a potential threat to crop growth and food safety. Thus far, few studies have focused on the phytoxicity of microplastics and As to leafy vegetable. In this study, we evaluated the single and combined toxicological effects of polymethyl methacrylate (PMMA) and As(V) on rapeseed (Brassia campestris L.). Single treatments of two sizes of PMMA particles, namely PMMA nano-plastics (PMMANPs) and PMMA micro-plastics (PMMAMPs) and As(V) significantly (P < 0.05) inhibited the germination index (GI) of rapeseed. The IC50 indicates that PMMANPs were more toxic than PMMAMPs. Combine-pollution experiments demonstrated that the GI, biomass, root length, and sprout length of the rapeseed under the combined treatment were lower than those subjected to As(V) or PMMANPs single treatment. Analysis of variance showed that the interaction effects of PMMANPs and As(V) for GI and root length were significant, and there was synergistic interaction between PMMANPs and As(V) on rapeseed germination. PMMANPs promoted the accumulation of As in sprouts under high As(V) concentrations (40 and 60 mg/L). The activities of lipase in rapeseed generally increased under single and combined treatments of As(V) and PMMANPs, and while α-amylase activities first increased and then decreased with the increase of PMMANPs. It appears that the combined stress of microplastics and As(V) exhibited synergistic interaction on the growth of rapeseed.

RNA-seq analysis revealed key genes associated with salt tolerance in rapeseed germination through carbohydrate metabolism, hormone, and MAPK signaling pathways

Seedling survival, growth, and yield are highly associated with seed germination performance. Germination of rapeseed differs under NaCl stress among cultivars, but the molecular mechanism of salinity tolerance in rapeseed is poorly understood. In the current study, in contrast to the trend of the hydrogen peroxide level, the activities of superoxide dismutase, catalase, and ascorbate peroxidase were increased in the salt-tolerant (Yangyou9; YY9) cultivar as compared with the salt-sensitive one (Zhongshuang11; ZS11). Integrative transcriptome analysis of four seed germination phases was carried out on the two rapeseed cultivars, which showed a substantial transcriptional change at 24 and 48 h. Different pairwise comparisons were performed to provide a comprehensive view of the biochemical processes that underpin salt tolerance and seed germination. Plant hormone signal transduction, MAPK signaling, glycolysis/gluconeogenesis, fermentation, and tricarboxylic cycle pathways were significantly enriched. Genes related to auxin (Aux) signaling, i.e., BnaAUX1, BnaTIR1, BnaGH3.5/17, and BnaSAUR were associated with salt tolerance through activating the germination process. Genes related to cytokinin (Ck; BnaAHP1/3/4 and BnaA-ARR3/4/8/9), gibberellin (GA; BnaPIF3/4/5), brassinosteroids (BRs; BnaBAK1, BnaBSK, BnaBZR1/2, BnaTCH4, and BnaCYCD3), and salicylic acid (SA; BnaNPR1/3/5, BnaPR1, and BnaTGA2/4) signaling transductions were linked to salt tolerance at the germination stage. While genes related to abscisic acid (ABA; BnaPP2C and BnaABF) and ethylene (Eth; BnaEIN3 and BnaERF1/2) were relatively upregulated in the dry seeds and salt-stressed sensitive cultivar, therefore, these genes may participate in salt sensitivity and seed dormancy. Moreover, the enzymatic activities (fructose-bisphosphate aldolase and isocitrate dehydrogenase) and content of phytohormones (indole-3-acetic acid (IAA), Ck, and GA) were significantly (p < 0.05) higher in YY9 than in ZS11, while ABA content showed a reverse trend, indicating the reliability of the RNA-seq data. Overall, this is the first study that provides new insights about transcripts involved in the salt tolerance during seed germination of rapeseed.

RATIONAL DRYING MODES FOR RAPE SEEDS

Studies on finding rational regimes for drying rapeseed based on drying kinetics and qualitative characteristics are presented. The value of the acid number of oil and laboratory germination of rapeseed on the 7th day of germination were taken as a qualitative characteristic. Experimental studies of the kinetics of the rapeseed drying process were performed on a convective drying stand using an automatic program for collecting and processing information, which makes it possible to obtain more accurate experimental data. According to the obtained experimental data with the help of an automatic program for collecting and processing information, graphs of the process kinetics from the influence of the coolant temperature are constructed. The presented graphs show the drying curves and drying speed curves that characterize the drying process that takes place during the period of falling drying speed. The study of the kinetics of the drying process of rapeseed in a single-stage mode at coolant temperatures of 50 - 80°C showed that for better quality it is necessary to use low-temperature modes. The acid number of oil in this temperature range does not change significantly and is 1.03 - 1.8 mg / g of oil. These values of the acid number of the oil correspond to the normative value. The greatest effect on the germination of rapeseed is observed from the temperature of the coolant. Increasing the temperature by 10 ° C in the temperature range from 50 to 80 ° C reduces the germination of the material. According to laboratory studies, we determined the drying regime of 50 ° С with a germination of 97% to be the most expedient and rational. All other drying regimes significantly reduce the germination of rapeseed and can not be recommended, as the germination value is less than the state standard of Ukraine 2240-93.

Effects of Selenium on Rape Seed Germination and Its Physiological and Biochemical Indexes

Effects of Selenium on Rape Seed Germination and Its Physiological and Biochemical Indexes

Germinating seed can sense low temperature for the floral transition and vernalization of winter rapeseed (Brassica rapa)

The hybrid production of winter rapeseed is limited by the difficult vernalization processes. Thus, floral regulation of winter rapeseed parental lines cannot be executed through selection of sowing time during hybrid production. Therefore, in this study, strong winter rapeseed was used as the material to analyse the floral transition mechanism of germinating seed vernalization. Results demonstrated that germinating seeds could sense low temperatures and complete vernalization following a low temperature treatment for 56.5 d with a 100 % vernalization rate. The regression equation between vernalization rate (y) and vernalization treatment days (x) was determined as y = 0.019x − 0.0765 (R² = 0.8529). When the vernalization treatment time was prolonged, the vernalization rate and fruiting ability increased rapidly, and variations were observed in the membrane lipid oxidation and physiological characteristics. Furthermore, at the prolonged treatment time of 10–50 d, the salicylic acid (SA) content continued to decrease, with values significantly lower than those of the control. SA content is significantly positively correlated with the level of BrFLC transcription and a significantly negatively correlated with the vernalization rate of germinating seeds. Moreover, the expressions of genes associated with SA biosynthesis, SA signal transduction, the flowering key negative regulators were suppressed and that of positive regulators were promoted during vernalization. These results suggest that SA as a floral repressor is involved in the regulation of the vernalization process of winter rapeseed germination seeds. In addition, SA may be related to the counting dosage of vernalization.

Influence of drying modes using microwave heating on enzymatic activity, germination energy and germination of rape seeds

Influence of drying modes using microwave heating on enzymatic activity, germination energy and germination of rape seeds

Magnetic Ti3C2 MXene functionalized with β-cyclodextrin as magnetic solid-phase extraction and in situ derivatization for determining 12 phytohormones in oilseeds by ultra-performance liquid chromatography-tandem mass spectrometry

Magnetic solid phase extraction integrated with in situ derivations for the profiling of 12 phytohormones in a single rapeseed seed was developed by using ultra-high performance liquid chromatography-tandem mass spectrometry. The Fe3O4@Ti3C2@β-cyclodextrin nanoparticles were firstly synthesized and used as an adsorbent for the solid-phase extraction of phytohormones. The magnetic dispersive solid-phase extraction and in situ derivation by the addition of N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide were ingeniously combined. This efficient pre-treatment method integrated the extraction, purification, and derivatization processes into one single step. Satisfactory methodological performance was achieved by optimization of the parameters. Linearities (R2 > 0.9928) and recoveries (80.4 %–115.1%) at three spiked levels, as well as the low matrix effect (from −16.63% to 17.06%) and limits of detection (0.89–13.62 pg/mL) were obtained. The spatio-temporal profiling of target phytohormones in different tissues of rapeseed germination was investigated. This method was successfully employed for analyzing target phytohormones in different oilseeds samples.