Germination Of Plants Research Articles

The Efficiency of Seed Priming with Dead Sea Water for Improving Germination and Early Seedling Growth of Wheat (Triticum aestivum L.) under Salinity

<p>Salinity is considered the most critical environmental factor which negatively affects the germination and growth of plants. In this study, the potential of using Dead Sea water (DS) as a seed priming agent for the mitigation of the adverse effects of salinity on seed germination and growth performance of wheat (<em>Triticum aestivum</em> L.) was investigated. Germination of wheat seeds primed with different doses of DS; 0%, 5%, 10%, 15%, and 20% were evaluated under different saline conditions (0, 100, 200, and 300 mM NaCl). High salinity (300 mM NaCl) remarkably inhibited germination attributes and reduced seedling length. However, seeds primed with DS exhibited improved germination parameters and seedling growth. Among the different DS concentrations used, the 10% DS priming achieved the highest increase in final germination percentage tolerance, germination index, relative germination salt tolerance, and seedling length. The increased tolerance to salinity was associated with improved water imbibition, α-amylase activity, antioxidant capacity and osmotic homeostasis correlated with high proline and soluble sugar levels. In addition, DS priming increased the membrane stability index, and reduced malondialdehyde content and K<sup>+</sup> leakage besides lowering Na<sup>+</sup>/K<sup>+</sup> ratio. Overall, priming with DS could be a promising strategy for minimizing the damaging effects of salinity in wheat.</p>

Dynamics of Scutellaria tuvensis (Lamiaceae) coenopopulations

The dynamics of density, abundance, ontogenetic structure and spatial organization of Scutellaria tuvensis coenopopulations under the influence of climatic factors in different habitat conditions has been studied. The decrease in the climate aridity in the basins of the Republic of Tuva in the last 10 years has a positive effect on the condition of the coenopopulations of S. tuvensis, occurring in dry and deseritied steppes. The coenopopulations of S. tuvensis are normal, stable, and in balance with the ecotope. The dynamics of the number, ecological density, spatial distribution of individuals in the coenopopulations, and the type of ontogenetic spectrum are influenced by climatic factors, while the values of demographic indicators in the coenopopulations are determined by the peculiarities of development of individuals in different habitats. Under a mildening climate (early warm spring and non-dry summer), optimal conditions are created for seed germination and further development of young plants. Recently, an increase in the number of young plants has been found in all habitats, a left-sided type of the ontogenetic spectrum is being formed, and coenopopulations are rejuvenating.

Thermal desorption remediation effects on soil biogeochemical properties and plant performance: Global Meta-analysis

Soil contamination remains a global problem, and numerous studies have been published for investigating soil remediation. Thermal desorption remediation (TDR) can significantly reduce the contaminants in the soil within a short time and consequently has been used worldwide. However, the soil properties respond to TDR differently and are dependent on the experimental set-up. The causative mechanisms of these differences are yet to be fully elucidated. A statistical meta-analysis was thus undertaken to evaluate the TDR treatment effects on soil properties and plant performance. This review pointed out that soil clay was reduced by 54.2%, while soil sand content was enhanced by 15.2% after TDR. This might be due to the release of cementing agents from clay minerals that resulted in the formation of soil aggregates. Soil electrical conductivity enhanced by 69.5% after TDR, which might be due to the heating-induced loss of structural hydroxyl groups and the consequent liberation of ions. The treatment of TDR leads to the reduction of plant germination rate, length, and biomass by 19.4%, 44.8%, and 20.2%, respectively, compared to that of control soil. This might be due to the residue of contaminants and the loss of soil fertility during the thermal process that inhibited plant germination and growth. Soil pH and sulfate content increased with heating temperature increased, while soil enzyme activities decreased with thermal temperature increased. Overall, the results suggested that TDR treatment has inhibited plant growth as well as ecological restoration.

Effect of Irrigation with Magnetized Water and Foliar Spraying with Humic Acid on Seedling Growth of Squash Plant (Cucurbita pepo L.) Under Salinity Stress

The experiment was conducted to investigate the effect of magnetic water (MW 14500 Gauss) and humic acid (HA 30 ppm) on the germination and growth of squash plants (Cucurbita pepo L.) exposed to salinity stress. Salinity stress was imposed by irrigation with saline water at concentrations of 1700, 2700, and 3700 ppm in addition to a control (tap water). The results showed that with increasing salinity stress, all vegetative growth parameters, seed germination ratio, and photosynthetic pigments decreased. On the other hand, foliar application of HA and irrigation with MW had a favorable impact on increasing vegetative traits and photosynthetic pigments. These results give a positive indication of the use of HA and MW to ameliorate the negative effects of salinity on squash plants.

Effect of Preliminarily Treating Lettuce Seeds with Hydrostatic Pressure and Cryofluids on the Germination and Subsequent Development of Plants

Effect of Preliminarily Treating Lettuce Seeds with Hydrostatic Pressure and Cryofluids on the Germination and Subsequent Development of Plants

Exploring New Ways for Cereal Planting: Implications of Different Algal Extracts and Hydrogel on Germination and Biochemical Content of Wheat Plants

Exploring New Ways for Cereal Planting: Implications of Different Algal Extracts and Hydrogel on Germination and Biochemical Content of Wheat Plants

Coping with salt stress.

Salt stress delays seed germination in plants by increasing the hydrolysis of arginine-derived urea.

Research on the influence of some types of water on seed germination in some vegetable plants

Numerous ancient cultures acknowledged the magnetic properties of magnets and utilized them as pain-relieving amulets. The Vedas, India's sacred books, discuss the use of magnets to control bleeding and treat specific female body ailments. Paracelsus, an alchemist and physician from the 16th century, discovered that magnets may cure most inflammations and ulcers, as well as many ailments of the bowel and uterus, and that they are beneficial for both internal and external illnesses. In the 18th century, Dr. Samuel Hahneman, the inventor of homeopathy, declared that "a magnetic wand can quickly and definitively cure a severe disease if it is brought close to the body." Magnetized water is also used well to treat assimilation issues (such as calcium and magnesium deficiencies and iron deficiency anemia). Magnetized water promotes early germination in the three species of legumes. The number of germinated seeds on the eighth day is the same for bean seeds watered with ozonated and magnetized water, and there are no significant variations between seeds irrigated with ozonated, filtered, and magnetized water.

Simulated Herbicide Drift Effects on Seed Germination, Seedling Emergence, and Seedling Growth of Native Plants of the Northern Great Plains.

Small concentrations of herbicide, such as those found in drift, can affect nontarget plants at different life-history stages including seed germination and seedling emergence as well as seedling growth. Fragmented landscapes, such as those in the northern Great Plains, lead to increased proximity of ecological restoration sites to agricultural lands using herbicides. Germination, emergence, and growth are crucial life-history stages leading to ecological restoration success, but these stages are sensitive to impacts from external factors such as herbicide exposure. A lab germination experiment and a greenhouse emergence experiment were performed to examine the effect of herbicides (2,4-dichlorophenoxyacetic acid [2,4-D], atrazine, and trifluralin) on species used in ecological restorations in the northern Great Plains. Seed germination, seedling emergence, and seedling growth of many study species decreased with exposure to herbicides at different concentrations representative of herbicide drift. At concentrations as low as 0.1% recommended application rate 2,4-D elicited broad effects on final seed germination percentage and germination rate. Atrazine affected seedling emergence and growth for a number of study species at concentrations as low as 10% recommended application rate. Trifluralin affected germination, emergence, and growth of the fewest number of study species. The information gained from these experiments can be used to inform restoration practitioners of best practices and recommended species to use when implementing ecological restoration adjacent to agricultural lands. Environ Toxicol Chem 2024;43:2387-2397. © 2024 The Author(s). Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Exploring the potential of ultrasound combined with sucrose treatments for germination and growth of sunflower sprouts

Ultrasound is a promising physical method for enhancing the germination of leguminous plants, while sucrose supplementation can boost plant growth and metabolism. However, limited studies have explored the synergistic effects and metabolic mechanisms of ultrasound and sucrose on sunflower sprouts. This study comprehensively investigated the impacts of ultrasound, sucrose, and their combination (US-Suc) on the germination and seedling growth of sunflower seeds. Overall, the combine treatment significantly increased the germination rate, length and weight of sunflower sprouts, compared to either individual treatment. Scanning electron microscope images showed that the US-Suc combination induced a distinct microstructural alteration, enhancing the water absorption capacity of sunflower seeds. Additionally, sunflower seeds exhibited increased activity of endogenous enzymes with antioxidant properties following US-Suc treatment. Lastly, Nuclear magnetic resonance-based metabolomics analysis demonstrated that the US-Suc treatment had a distinct regulatory impact on the metabolic profile compared to either treatment alone. The metabolism of carbohydrates and phenolic compounds was significantly up-regulated upon US-Suc treatment, leading to improved plant growth and resistance to environmental stress. In conclusion, pre-treating with ultrasound in combination with external sucrose application showed great promise in seed germination, enhancing nutrient accumulation in sunflower sprouts by accelerating water uptake and boosting enzyme activity. Thus, US-Suc represents an effective approach to increase both yield and nutritional value of sunflower sprouts.

The effect of biochar obtained from waste filter coffee grounds on plant germination

Nowadays, coffee consumption is quite high, and the consumption of filter coffee is steadily increasing. Consequently, there is a significant increase in waste filter coffee. This study aims to evaluate waste filter coffee grounds using a zero-waste approach. In this context, the solid product of pyrolyzed waste filter coffee grounds was added to the soil in specific ratios to improve soil quality and increase yield. The effects on the root and stem development of arugula (Eruca vesicaria) and garden cress (Lepidium sativum) plants were investigated. Waste filter coffee grounds was homogeneously mixed with soil at application rates of 1, 2, and 4 tons/ha. The results of the study observed that the pyrolysis solid product positively affected plant growth. Comparing the data, the highest yield in plants was observed in soil with added biochar, while lower yields were seen in soil with added raw waste filter coffee grounds, and the lowest yield was found in soil without biochar. Among the soils with added biochar, the most significant root and stem development was observed in plants with 2 tons/ha of added biochar.

Isolation, screening, and molecular characterization of indigenous Trichoderma isolates from West Java, Indonesia and their plant growth-promoting capability on rice plants (Oryza sativa L.)

Trichoderma species are widely acknowledged as growth-promoting fungi that have been utilized to enhance the growth and yield of numerous crops. This research examined the capacity of 30 Trichoderma strains, isolated from two organic rice fields in West Java, Indonesia, to enhance the germination, growth, and physiological characteristics of rice plants (Oryza sativa L.). In general, Trichoderma strain TM10 demonstrated the greatest ability to increase seed germination (97.25 %), vigor index (3122.83), and germination speed (59.91 seeds/day). This strain also increased seedling root length and seedling height by 101.62 % and 112.20 %, respectively. Plants treated with TM10 exhibited a notable improvement in root length, plant height, fresh weight, and dry weight compared to control plants, demonstrating increases of 188.68 %, 69.90 %, 157.41 %, and 159.38 %, respectively. Furthermore, the total chlorophyll content and stomatal number in TM10-treated plants exhibited increments of 75.23 % and 75.53 %, respectively. Five selected isolates (TM7, TM10, SB2, SB8, and SB14) were evaluated for their potential to produce plant growth-promoting compounds, including phosphatase enzyme (ranging from 0.54 to 11.14 µg pNP g−1h−1), indole-3-acetic acid (IAA) (ranging from 28.96 to 63.91 µg/mL), ammonia (ranging from 1.96 to 5.79 µg/mL), and hydrogen cyanide (HCN) (ranging from 221.76 to 274.82 ppm). The best strain, namely TM10, was then molecularly identified as Trichoderma yunnanense. This investigation demonstrates that Trichoderma spp. isolated from organic rice fields could be used as a bioinoculant in sustainable rice production.

Salicylic acid, ascorbic acid and wood vinegar effects on fenugreek (Trigonella foenum-graecum L.) plant germination at various salt concentrations

Salicylic acid, ascorbic acid and wood vinegar effects on fenugreek (Trigonella foenum-graecum L.) plant germination at various salt concentrations

Germination of 10 midland plant species from the eastern Mediterranean Basin: effects of smoke, syringaldehyde, karrikinolide and cyanohydrin

The beneficial effects of smoke and its constituents, karrikinolide (KAR1) and cyanohydrin glyceronitrile, on the germination of Mediterranean lowland species are well‐documented. However, very little is known about the role of these signals on the germination of plants at higher altitudes. In addition, lignin‐derived chemicals, such as syringaldehyde (SAL), have recently been proposed as overlooked cues for smoke‐induced germination. To address these gaps in the literature, we investigated the effects of smoke‐water and SAL on the germination of 10 midland species growing on serpentine soils. We also sought to determine whether SAL interacts with KAR1 and/or mandelonitrile (MAN, a cyanohydrin) to enhance seed germination. The results show that smoke‐water significantly improved the germination of three species (Barbarea duralii, Digitalis cariensis and Turritis laxa). SAL, on the other hand, had no positive effect on the germination of the 10 species tested. Furthermore, three smoke‐sensitive species and Verbascum cariense responded to KAR1 and/or MAN. Finally, SAL did not exert any synergistic effects on germination in interaction with KAR1 and MAN. In conclusion, we provide evidence that smoke is an important germination cue also for Mediterranean midland species. Moreover, SAL did not play a role in stimulating germination in smoke‐sensitive species, either independently or in combination with other smoke chemicals.

Ecotoxicity of Tar from Coffee Grounds and Pine Pellet Gasification Process

This study determined the toxicity of the condensates produced during the gasification of two waste types. Coffee grounds, pine pellets, and a mixture of both substrates at a ratio of 1:1 were used in the study. Two microbiotests were applied for soil plants and aquatic macrophytes, and quantitative analysis of the soil microbiome for primary taxonomic groups of microorganisms was conducted. Three contamination rates were used in the Phytotoxkit test and the microbiological tests, 100, 1000, and 10,000 mg·kg−1 d.m. of soil, while in the aquatic organism studies, successive two-fold serial dilutions of condensates were used. The presence of liquid waste from the gasification process adversely affected the germination and development of terrestrial plants and the vegetative growth of aquatic plants. The condensate components modified the composition of the soil microbiome, adversely affecting soil fertility. The negative impact increased with increasing levels of contamination and primarily depended on the type of substrate from which the gasification process produced the liquid waste.

Arbuscular mycorrhizal fungi affect early phenological stages of three secondary vegetation species in a temperate forest

The relationship between arbuscular mycorrhizal fungi (AMF) and secondary vegetation (SV) species at early phenological stages is critical for the successful establishment of these plants on disturbance sites in temperate forests. The main objective of this research is to evaluate the effect of AMF colonization on the early phenological stages (germination and early growth) of three shrub species present in the SV of a temperate forest in central Mexico. We collected soil from different sites in the Abies religiosa forest in central Mexico. We collected seeds of Acaena elongata, Ageratina glabrata, and Solanum pubigerum. We used a controlled experimental design with pasteurized soil (-AMF treatments) and unpasteurized soil (+ AMF treatments). We monitored germination percentage, growth (shoot and root weight and total biomass), AMF root colonization, and the mycorrhizal response index (MRI) for each plant species. All three species tested benefited by AMF, showing higher germination rates. Shoot and root weight and total biomass were significantly higher in the + AMF treatment. Solanum pubigerum showed greater stem length and Ageratina glabrata showed greater root development due to AMF. Ageratina glabrata and Acaena elongata were the most responsive to AMF as indicated by MRI. This research underscores the critical role of AMF in the early phenological stages of SV and highlights the potential ecological benefits of AMF in supporting plant germination and plant growth. These results suggest that AMF enhance germination and early growth of secondary vegetation species, which can be considered in management plans for forest ecosystems.

Roles of Endophytic Fungi Isolated from Mangifera indica L. in Promoting Plant Growth.

Endophytic fungi have been shown to synthesize bioactive secondary metabolites, some of which promote plant growth through various mechanisms. In our previous study, endophytic fungi were isolated from mango trees (Mangifera indica L.). The present study examined fifty endophytic fungal isolates for mineral solubilization activity, ammonia production, and siderophore production. It was shown that these isolates could produce phytohormones indole-3-acetic acid and gibberellic acid, as well as inhibit plant pathogens, specifically Colletotrichum gloeosporioides and Lasiodiplodia theobromae. The results showed that all the isolated fungal endophytes exhibited various activities. Based on the findings, two fungal endophytes-Aureobasidium pullulans CY.OS 13 and Aspergillus tamarii CY.OS 144-were selected for dual inoculation in chili plants under pot-scale conditions to investigate their potential to improve growth-related traits such as seed germination, shoot and root length, biomass, and chlorophyll content. Seed treated with A. pullulans CY.OS 13 and/or A. tamarii CY.OS 144 showed a significant (p < 0.05) increase in seed germination and growth parameters of chili plants grown under pot-scale conditions. Particularly, chili plants whose seeds were injected with a combination of the two selected endophytic fungi showed the highest plant development traits. Therefore, the selected endophytic fungi have the potential to be used as biofertilizers, especially when combined. They could eventually replace chemical fertilizers because they are environmentally friendly, beneficial to humans, and can even promote sustainable agriculture.

Investigating the Impact of Microplastics Type of Polyethylene, Polypropylene, and Polystyrene on Seed Germination and Early Growth of Rice Plants

ABSTRACTMicroplastics (MPs), which are emerging pollutants in terrestrial ecosystems, could potentially impact plant growth. One of the least explored aspects of crop development is seed germination, a crucial stage in the plant's lifecycle. This study conducted soil cultivation trials to investigate the effects of 1% (w/w) concentrations of polyethylene (PET), polypropylene (PP), and polystyrene (PS) MPs on rice growth over a 15‐day period. Parameters such as fresh weight, shoot height, root length, chlorophyll levels, and MPs identification in the roots were observed. The findings revealed the accumulation of 6–9 MP items in the roots. PET MPs inhibited root elongation (15%–20%), reduced shoot height (15%–30%), and decreased rice fresh weight (12%–37%). MPs PET, PP, and PS contamination reduced the content of chlorophyll a (15%–43%), b (21%–41%), and total (11%–40%) in rice leaves. This study enhances our understanding of the ecotoxicological effects of these three types of MPs on rice. The utilization of this data will further inform our understanding of MPs' behavior in soil vegetation and provide valuable insights into their land‐based impacts.

MYB4 in Lilium pumilum affects plant saline-alkaline tolerance

ABSTRACT Lilium pumilum DC (L. pumilum DC) plays an important role in the rational utilization of salinized soil. To explore the molecular mechanism of salt-tolerant L. pumilum, the LpMYB4 was cloned. LpMYB4 close relationship with Bambusa emeiensis and Zea mays MYB4 throughout the phylogenetic tree construction. LpMYB4 protein was found to be localized in the nucleus. Prokaryotic and eukaryotic bacterial solution resistance experiments proved that the exogenous introduction of LpMYB4 made the overexpression strains obtain better survival ability under saline-alkaline stress. Compared with wild-type plants, tobacco plants overexpressing LpMYB4 had better growth and lower leaf wilting and lodging, the content of chlorophyll was higher, the content of hydrogen peroxide and superoxide anion was lower, the activity of peroxidase and superoxide dismutase was higher and the relative conductivity was lower under saline-alkaline stress. The analysis of seed germination and seedling resistance of transgenic plants under salt stress showed that LpMYB4 transgenic seeds were more tolerant to salt stress during germination and growth. Yeast two-hybrid and two-luciferase complementation experiments showed that LpMYB4 interacted with yeast two-hybrid and LpGPX6. The analysis of the role of LpMYB4 in improving plant saline-alkali resistance is helpful to the transformation of plant germplasm resources and has great significance for agriculture and sustainable development.

Containing alien plants in coastal dunes: Evidence from a soil manipulation experiment

Biological invasion is recognised as one of the major threats to biodiversity, particularly in disturbance-prone ecosystems such as costal dunes. Many studies have associated alien plant invasion of dune ecosystem to human disturbances, but less is known about the role of soil properties in invasion after disturbance. Soil properties are crucial filters during plant succession and soil-related changes in the initial stage of species colonization might shape the final success of the invaders.We performed a manipulative experiment aimed at elucidating the effects of soil properties on plant colonization processes in highly invaded dune systems, as a proxy for plausible management actions to curb the success of exotic plant species over native ones, which was measured through species richness and abundance.In a barrier island of the Marano and Grado lagoon, Northern Adriatic Sea, we mechanically removed all the native and alien vegetation present in the back dune (also known as secondary dune), triggering a new ecological succession and further altered, for the following three months, soil properties by adding salt, nitrogen, and organic matter in a full factorial design with randomized blocks.The soil treatments reduced the overall species richness and abundance of alien plants. Further, soil treatment interactions strongly shaped community evenness and species richness. Soil salinity had a positive effect on native cover while decreasing the overall number of alien species, especially in soil with added organic matter.Our findings suggest that soil salinity, and its interplay with organic matter, might significantly reduce the initial success of alien species propagule pressure (i.e. alien plant germination), with likely implications for the trajectories of future plant communities. This study highlights that alien plant containment should be focused on early stages of succession, giving new perspective on future environmental management actions for dune restoration and conservation.