• ICESat-2 has the potential to improve the estimation accuracy and efficiency of forest aboveground biomass on a large scale. • The Google Earth Engine cloud platform was used for Sentinel-2 images acquisition and composition. • The optimized extreme learning machine achieved the highest estimation accuracy and reasonable forest aboveground biomass spatial distribution. Natural forests have the most complex structure and richest biodiversity among terrestrial ecosystems and are essential for maintaining the carbon balance and stability of the biosphere. Aboveground biomass (AGB) is a primary indicator used to evaluate forests and can directly measure forest growth and the quality of natural forests. Accurate and rapid AGB estimations can significantly improve the efficiency of forest management and deepen the understanding of the forest carbon cycle. The Ice, Cloud, and Land Elevation Satellite-2 (ICESat-2), one of the most recently launched spaceborne light detection and ranging (LiDAR) instruments, can penetrate forest canopies and obtain accurate, large-scale forest vertical parameter measurements. However, the discrete footprints offered by ICESat-2 cannot provide comprehensive spatial AGB distribution data. In this study, an optimized extreme learning machine (ELM) method was proposed to estimate the AGB of natural forests on the eastern Qinghai-Xizang Plateau, China. The synthetic Sentinel-2 images acquired from the Google Earth Engine (GEE) were synergized with ICESat-2 to realize continuous AGB mapping. To verify the effectiveness of the optimized method, support vector machine (SVM), k-nearest neighbor (kNN), and backpropagation (BP) neural network models were also established for comparison. The measured AGB data extracted from the forest management inventory (FMI) were used for model accuracy evaluation. The results show that the optimized ELM achieved the best estimation effect among all the analyzed models, with an R 2 value of 0.68 and a root mean square error (RMSE) value of 25.14 mg/ha. The optimized ELM obtained the minimum RMSE and greatly improved the AGB prediction efficiency. These findings prove the ability of ICESat-2 in estimating the AGB of natural forests, which can provide a new way for large-scale forest resources investigation in high-altitude areas with a harsh environment.