Abstract: The present study investigates the influence of bacteria on varying binder constituents to enhance the workability, strength, and microstructure properties of self-consolidating geopolymer concrete (SCGC). Geopolymer concrete is a sustainable alternative to conventional cement-based concrete, as it utilizes industrial by-products and reduces carbon emissions. The bacterial strains, known for their ability to promote mineral precipitation and improve concrete properties, are introduced in varying proportions to the geopolymer binder constituents. The goal of this experimental investigation is to better understand how Bacillus Cohnii bacteria affect the workability and strength of alkali-activated Self-consolidating Concrete (SCC) mix compositions. Three mix formulations with variable binder contents in the range of 400 kg/m3 and 450 kg/m3 were the subject of experimental research. This research work aims on investigating various binder constituents on Strength, Workability and Microstructure. Three different SCGC mixes were prepared by varying the percentage fly ash and Alcofine in range of 0%, 10%, 30% and 0%, 5%, 5% to the quantity of GGBFS respectively. Alcofine increases final setting time. For all mixes, ambient curing was done. As per EFNARC recommendations, the fresh qualities of SCGC were determined using the Slump test, T50 slump test, L-Box test, V- funnel test, and J-ring test. By conducting compression tests, split tensile tests, and flexure tests after 7, 14 and 28 days, the strength characteristics of SCGC were identified. The SCGC's microstructure and chemical composition were both determined by SEM and EDS analyses, respectively. According to test results, an alkaline solution sample with a 13M concentration and 450 kg/m3 of binder produced the highest compressive, flexural, and split tensile strengths after 28 days producing values of 58.32 MPa, 3.5 MPa, and 4.27 MPa, respectively.