PurposeTo investigate changes in both lens shape and the epithelial distribution as emmetropia is established in C57Bl6J mice between postnatal weeks 4 to 6.MethodsWe used a computational approach to produce geometrical maps of the mouse lens and its epithelium. Conveniently our approach ulitises data collected from intact lenses imaged using a conventional laser scanning confocal microscope. It delivers cartesian coordinates for every epithelial cell in the lens epithelium. We chose to investigate lenses during postnatal weeks 4 to 6, a time during lens development when emmetropia is established in this inbred strain of mice (Tkatchenko et al., 2010).ResultsGeometric mapping standardises the measurement and analysis of lens epithelial cell densities. Emmetropia is established in C57Bl6J inbred mice, but many other strains exhibit considerable refractive error (Tkatchenko et al., 2019). The eye lens plays its part in the development and establishment of emmetropia in mice (Tkatchenko et al., 2010; Pardue et al., 2013). We record a change in the shape of the eye lens from a spheroid to a lentoid shape between postnatal weeks 4 and 6. The distribution of the epithelial cells in the lens epithelium also changes from a non‐zonal to a zonal distribution, with characteristics that typify the epithelia of adult animals. We correlate these observations regarding lens geometry with genetic mutations in lens cytoskeletal proteins found in those strains that present with refractive error, but which are absent from C57Bl6J (Sandilands et al., 2004). We discuss the potential significance of these observations in the context of the mechanistic basis of the lens contribution to emmetropia.ConclusionsThe development of emmetropia in the C57Bl6J inbred strain of mice correlates with changes in epithelial cell organisation and changes in lens geometry, which when combined with strain‐specific genetic differences offer a mechanistic understanding involving the lintermediate filament cytoskeleton.Pardue, M. T., et al., (2013) Exp Eye Res, 114, 96–105.Sandilands, A., et al (2004). Eye Res. 78, 109–123.Tkatchenko, T. V. et al. (2019). BMC Medical Genomics, 12, 1–24.Tkatchenko, T. V., Shen, Y. and Tkatchenko, A. V. (2010) Invest Ophth Vis Sci, 51, 21–27. https://doi.org/10.1167/iovs.08‐2767