Precise stratigraphic chronology is the basis of many studies (e.g., tectonic, paleoclimate, and mineralization) in geoscience. Here, we carried out a detailed youngest detrital zircon U-Pb age-constrained magnetostratigraphic study on the middle-lower parts of a 2309-m-thick Upper Jurassic−Lower Cretaceous sequence from west Jiangcheng in the southern Simao Basin, southeastern Tibetan Plateau. A total of 2262 paleomagnetic samples were collected for magnetostratigraphy, and five siltstone/sandstone samples were collected for detrital zircon U-Pb analyses. Progressive thermal and alternating field demagnetizations isolated 1575 well-defined interpreted primary characteristic remanent magnetization directions, which yielded 25 normal polarity (N7−N31) and 25 reverse polarity (R8−R32) magnetozones. The interpreted maximum depositional ages calculated using the youngest detrital zircon U-Pb dates are 159.0 Ma, 154.0 Ma, 161.0 Ma, 149.3 Ma, and 139.5 Ma for the stratigraphic horizons at 2303 m, 1986 m, 1600 m, 1350 m, and 1110 m, respectively, in the section. Together with the previous magnetostratigraphic results of the upper part of the section, the observed polarity zones are best correlated with chrons C34n−M17r of the geomagnetic polarity timescale (GPTS2012), yielding magnetostratigraphic age estimates of ca. 143.5 Ma to ca. 65.0 Ma for the entire stratigraphic sequence. Paleogeographic analysis shows that the Simao Basin was in a low-latitude offshore environment during the Late Jurassic−earliest Cretaceous (before 142.3 Ma), a dominant fluvial environment during the early−middle Early Cretaceous (142.3−111.5 Ma), with widespread desertification at the end of this period, a saline lake sedimentary environment during the late Early Cretaceous−early Late Cretaceous (111.5−88.7 Ma), and a fluvial environment during the late Late Cretaceous (88.7−65.0 Ma). The changes in depositional environment are consistent with regional/global tectonic and paleoclimate changes, such as the dismission of Pangea, the termination of the megamonsoon, and the closure of the Meso-Tethys Ocean.