Geological Events Research Articles

Diversity and biogeography of South American mud turtles elucidated by multilocus DNA sequencing (Testudines: Kinosternidae)

Kinosternon is the most speciose genus of extant turtles, with 22 currently recognized species, distributed across large parts of the Americas. Most species have small distributions, but K. leucostomum and K. scorpioides range from Mexico to South America. Previous studies have found discordance between mitochondrial and nuclear phylogenies in some kinosternid groups, with the current taxonomy following the nuclear-based results. Herein, based on extended molecular, geographic, and taxonomic sampling, we explore the phylogeographic structure and taxonomic limits for K. leucostomum and the K. scorpioides group and present a fossil-calibrated nuclear time tree for Kinosternon. Our results reveal contrasting differentiation patterns for the K. scorpioides group and K. leucostomum, despite overlapping distributions. Kinosternon leucostomum shows only shallow geographic divergence, whereas the K. scorpioides group is polyphyletic with up to 10 distinct taxa, some of them undescribed. We support the elevation of K. s. albogulare and K. s. cruentatum to species level. Given the deep divergence within the genus Kinosternon, we propose the recognition of three subgenera, Kinosternon, Cryptochelys and Thyrosternum, and the abandonment of the group-based classification, at least for the K. leucostomum and K. scorpioides groups. Our results show an initial split in Kinosternon that gave rise to two main radiations, one Nearctic and one mainly Neotropical. Most speciation events in Kinosternon occurred during the Quaternary and we hypothesize that they were mediated by both climatic and geological events. Additionally, our data imply that at least three South American colonizations occurred, two in the K. leucostomum group, and one in the K. scorpioides group. Additionally, we hypothesize that discordance between mitochondrial and nuclear phylogenetic signal is due to mitochondrial capture from an extinct kinosternine lineage.

Complete Mitochondrial Genome of Four Peristediidae Fish Species: Genome Characterization and Phylogenetic Analysis.

The systematic revision of the family Peristediidae remains an unresolved issue due to their diverse and unique morphology. Despite the popularity of using mitochondrial genome research to comprehensively understand phylogenetic relationships in fish, genetic data for peristediid fish need to be included. Therefore, this study aims to investigate the mitochondrial genomic characteristics and intra-family phylogenetic relationships of Peristediidae by utilizing mitochondrial genome analysis. Therefore, this study aims to investigate the phylogenetic relationship of Peristediidae by utilizing mitochondrial genome analysis. The mitochondrial genome of four species of Peristediidae (Peristedion liorhynchus, Satyrichthys welchi, Satyrichthys rieffeli, and Scalicus amiscus) collected in the East China Sea was studied. The mitochondrial gene sequence lengths of four fish species were 16,533 bp, 16,526 bp, 16,527 bp, and 16,526 bp, respectively. They had the same mitochondrial structure and were all composed of 37 genes and one control region. Most PCGs used ATG as the start codon, and a few used GTG as the start codon. An incomplete stop codon (TA/T) occurred. The AT-skew and GC-skew values of 13 PCGs from four species were negative, and the GC-skew amplitude was greater than that of AT-skew. All cases of D-arm were found in tRNA-Ser (GCT). The Ka/Ks ratio analysis indicated that 13 PCGs were suffering purifying selection. Based on 12 PCGs (excluding ND6) sequences, a phylogenetic tree was constructed using Bayesian inference (BI) and maximum likelihood (ML) methods, providing a further supplement to the scientific classification of Peristediidae fish. According to the results of divergence time, the four species of fish had apparent divergence in the Early Cenozoic, which indicates that the geological events at that time caused the climax of species divergence and evolution.

Subsurface stratigraphy suggested by the layered ejecta craters in the Martian northern planitiae

The formation of layered ejecta craters (LECs) is generally thought to be related to the subsurface water-ice. The distribution of LECs in the northern regions of Mars (0 to 70° N) features both local and latitudinal variations, e.g., there are generally few LECs within volcanic areas, and the observed minimum and median diameters of the LECs decreases with increasing latitude. The observed minimum diameters of the LECs in the six planitiae within the northern lowlands suggest the roof depth of the LECs-forming layer has clear lateral variability, ranging between ∼90 m in the Arcadia Planitia and ∼280 m in the Isidis Planitia. The observed LECs are suggested to mainly form during the Amazonian, implying that the LEC-forming water-ice-bearing strata might be still present. The variation might reflect the comprehensive influence on the subsurface water-ice by the climate conditions and geologic events during the Amazonian period. The proposed subsurface stratigraphies in the six planitiae suggest that the total thickness of the LECs-related strata in the Chryse Planitia is the largest (∼4.6 km) and in the Arcadia Planitia is the smallest (∼1.6 km). There could be as much as ∼3.5–16 million km3 subsurface water stored in these strata, resulting in a ∼24–110 m global equivalent layer (GEL) of water. Given that water is a fundamental ingredient to life, its migration from surface to subsurface shifts the focus of its exploration. Both Arcadia and Utopia Planitiae represent ideal candidates as locations where both shallow and deep subsurface water-ice might be abundant, they represent high-priority targets for future missions at mid-latitudes for biosignature searching and in-situ resource utilization.

Thermal stability of the electron spin resonance signal of modern continental barite

The electron spin resonance (ESR) method has been successfully applied to dating hydrothermal barite; however, there have been few studies of modern continental barite, such as that generated by fault movement or in sedimentary deposits. To better understand the geological processes involved in the formation of continental barite, its thermal characteristics and age firstly need to be defined. In this study, one fault barite sample and two sedimentary barite samples were obtained from continental sites in China for ESR signal thermal stability experiments. Microwave-power experiments indicate that 0.5 mW produces the maximum ESR signal intensity and the highest signal-to-noise ratio. Stepwise-heating results reveal that ESR signal intensities remain stable below 100 °C then show an increasing trend that peaks at ∼180 °C, following which they decrease rapidly between 180 and 320 °C and disappear at ∼380 °C. Isothermal heating results show that ESR signal intensities remain stable within a temperature range of 100–200 °C but show a decreasing trend for temperatures of 240–320 °C. These results indicate that the thermal decay of the ESR signal intensity for the three studied continental barites follows second-order decay kinetics. The thermal lifetimes calculated for the three samples are 6.3 × 108, 7.5 × 107, and 8.7 × 106 a, respectively. Thus, barite ESR dating can be used to date related geological events since early Pleistocene.

Cicada minimum age tree: Cryptic speciation and exponentially increasing base substitution rates in recent geologic time

We developed a new time-calibrated tree incorporating primarily endemic along with some cryptic Ryukyu islands cicada data, following the recent publication of global cicada data by Marshall et al. (2018), Łukasik et al. (2018), Simon et al. (2019), Price et al. (2019), and Hill et al. (2021). A total of 352 specimens were analyzed using BEAST v1. X software with a relaxed clock model. Fossil calibrations as old as Triassic were adopted largely following Johnson et al. (2018) and Moulds (2018), and a Quaternary geological event calibration was adopted following Osozawa et al. (2012, 2021b) and input into BEAST v1. X. Our timetree suggests that Tettigarctidae had a cicada basal lineage as old as 200.63 Ma, with Derotettiginae the next oldest lineage at 99.2 Ma. Tibicininae is a sister of the remaining subfamilies of Tettigomyiinae, Cicadettinae, and Cicadidae, and their species level differentiation and radiation began at 40.57 Ma. The Cicadinae clade consists of specific tribes with paraphyletic relationship, and the vicariance and adaptive radiation generated many cryptic species in each tribe. We estimated base substitution rate as a function of age, and the result strongly indicates an exponential increase of base substitution rate in recent geologic time. The consequent increase in cicada biodiversity, including generation of cryptic species in the Ryukyu Islands and surroundings, may have been driven by the generation and spreading of C4 grasses and coeval Quaternary climate change.

Genetic structure of ten Artemia populations from China: cumulative effects of ancient geological events, climatic changes, and human activities

This study investigated the population genetics and distribution patterns of Artemia populations from ten inland salt lakes in China. A total of 1,274,698 Artemia single nucleotide polymorphisms (SNPs) were identified. The results showed that these populations could be geographically and genetically divided into four distinct groups, and that the Tibetan populations were further divided into two subpopulations with a trend of decreasing genetic diversity from west to east. The Tibetan population had the highest genetic diversity, whereas the Shanxi population had the lowest. There was moderate genetic differentiation between the Tibetan populations and greater genetic differentiation between the Xinjiang and Shanxi populations. IBD (isolation by distance) suggested that geographical isolation contributes to genetic differentiation. In addition, there was some degree of gene flow among the ten populations, with A. sinica showing unidirectional gene flow in all populations in the eastern Nagri region. Species distribution modeling showed that mean annual temperature, temperature seasonality, and annual precipitation were the main environmental factors affecting the distribution of Artemia populations and suitable habitats for Tibetan populations will be further reduced in the future. It is necessary to strengthen the protection of germplasm resources and formulate scientific protocols for the sustainable development and utilization of Artemia resources.

Multi-parameters monitoring of geological disasters around the Taihang Mountains-Yanshan area, Hebei Province

The Hebei Taihang Mountains-Yanshan region marks North China’s natural boundary between plains and plateaus. The unique geological and geographical conditions have led to a hazardous environment with frequent geological events. Hence, conducting a comparative analysis of regional geological hazard drivers is necessary. A geological hazard driver analysis model using multiparameter preferential Geodetectors was developed, focusing on the Taihang Mountains-Yanshan region in Hebei. It was divided into zones: Taihang Mountains, Yanshan Mountains and Dam Plateau. The results showed significant differences in the explanatory power of geohazard drivers in various zones, varying with the softening of the natural geological environment and increased human activities. In the Taihang Mountains, the primary geohazard factor is average annual rainfall, whereas in the Yanshan Mountains, it is average annual rainfall and geomorphology. Interactions among factors reveal that annual average rainfall holds the highest explanatory power for geological hazard spatial differences, notably interacting with geomorphological, geological and human activity regions, yielding an escalating effect. The study findings can be crucial references for disaster prevention, mitigation, national land resource development, economic planning and the ‘six belts’ construction in Beijing, Tianjin and Hebei.

Botany and geogenomics: Constraining geological hypotheses in the neotropics with large-scale genetic data derived from plants.

Decades of empirical research have revealed how the geological history of our planet shaped plant evolution by establishing well-known patterns (e.g., how mountain uplift resulted in high rates of diversification and replicate radiations in montane plant taxa). This follows a traditional approach where botanical data are interpreted in light of geological events. In this synthesis, I instead describe how by integrating natural history, phylogenetics, and population genetics, botanical research can be applied alongside geology and paleontology to inform our understanding of past geological and climatic processes. This conceptual shift aligns with the goals of the emerging field of geogenomics. In the neotropics, plant geogenomics is a powerful tool for the reciprocal exploration of two long standing questions in biology and geology: how the dynamic landscape of the region came to be and how it shaped the evolution of the richest flora. Current challenges that are specific to analytical approaches for plant geogenomics are discussed. I describe the scale at which various geological questions can be addressed from biological data and what makes some groups of plants excellent model systems for geogenomics research. Although plant geogenomics is discussed with reference to the neotropics, the recommendations given here for approaches to plant geogenomics can and should be expanded to exploring long-standing questions on how the earth evolved with the use of plant DNA.

A revised phylogeny of Boletaceae using whole genome sequences

ABSTRACT The porcini mushroom family Boletaceae is a diverse, widespread group of ectomycorrhizal (ECM) mushroom-forming fungi that so far has eluded intrafamilial phylogenetic resolution based on morphology and multilocus data sets. In this study, we present a genome-wide molecular data set of 1764 single-copy gene families from a global sampling of 418 Boletaceae specimens. The resulting phylogenetic analysis has strong statistical support for most branches of the tree, including the first statistically robust backbone. The enigmatic Phylloboletellus chloephorus from non-ECM Argentinian subtropical forests was recovered as a new subfamily sister to the core Boletaceae. Time-calibrated branch lengths estimate that the family first arose in the early to mid-Cretaceous and underwent a rapid radiation in the Eocene, possibly when the ECM nutritional mode arose with the emergence and diversification of ECM angiosperms. Biogeographic reconstructions reveal a complex history of vicariance and episodic long-distance dispersal correlated with historical geologic events, including Gondwanan origins and inferred vicariance associated with its disarticulation. Together, this study represents the most comprehensively sampled, data-rich molecular phylogeny of the Boletaceae to date, establishing a foundation for future robust inferences of biogeography in the group.

Subaerial volcanism broke mid-Proterozoic environmental stasis.

The mid-Proterozoic, spanning 1.8 to 0.8 billion years ago, is recognized as a phase of marine anoxia, low marine primary productivity (MPP), and constrained eukaryotic biodiversity. However, emerging evidence suggesting intermittent environmental disturbances and concurrent eukaryotic evolution challenges the notion of a stagnant Earth during this era. We present a study detailing volcanic activity and its consequential impact on terrestrial weathering and MPP, elucidated through the examination of 1.4-billion-year-old tropical offshore sediments. Our investigation, leveraging precise mercury (Hg) and lithium (Li) isotopic analyses, reveals the introduction of fresh rock substrates by local volcanism. This geological event initiated a transformative process, shifting the initial regolith-dominated condition in tropical lowland to a regime of enhanced chemical weathering and denudation efficiency. Notably, the heightened influx of nutrient-rich volcanic derivatives, especially phosphorus, spurred MPP rates and heightened organic carbon burial. These factors emerge as potential drivers in breaking the long-term static state of the mid-Proterozoic.

Andean uplift, climatic events, and rainforest bridges determined the spatiotemporal evolution of the cumaru and tonka beans (Dipterygeae: Leguminosae)

Abstract Historical climatic and geological events are largely known to have impacted biome evolution across the Neotropics and their associated rich biodiversity. However, a clear understanding of dispersal and biome shifts between neotropical biomes over time is still lacking for many ecologically important plant clades. We investigated the timing and ancestral species movements within the early branching papilionoid legume Dipterygeae lineage of neotropical tree species, some of which are among the most ecologically dominant across Amazonian rainforests and Brazilian savannas, to understand how dispersal and evolutionary trajectories in biome predilection have led to their modern distribution. We also estimated the evolution of fruit types and discuss their potential roles in the colonization of non-Amazonian rainforest habitats. To address these questions, we used one nuclear (ITS/5.8S) and two plastid (matK and trnL intron) DNA regions of the Dipterygeae clade to estimate divergence times with a Bayesian relaxed-clock approach, ancestral area distribution, and evolutionary shifts in biome and morphological traits. All currently recognized species of this clade were sampled, covering its full geographical range. Dipterygeae originated in the Amazonia during the Middle Eocene, where it has undergone a long history of in situ diversification, particularly over the last 15 Myr. Dispersal events into the rainforests of the Brazilian Coastal Atlantic Forest and Chocó + Central America, the fire-prone savannas of the Cerrado, and the Caatinga seasonally dry tropical forest occurred independently. Most lineages ecologically associated with rainforests are dispersed by animals (zoochory), whereas shifts to open habitats generally coincide with wind dispersal (anemochory). Our study highlights the significance of geological events, climatic factors, and shifts in fruit type as crucial contributors to the diversification of Dipterygeae.

Identification of Precursors in InSAR Time Series Using Functional Data Analysis Post-Processing: Demonstration on Mud Volcano Eruptions

One of the most promising applications of satellite data is providing users in charge of land and emergency management with information and data to support decision making for geohazard mapping, monitoring and early warning. In this work, we consider ground displacement data obtained via interferometric processing of satellite radar imagery, and we provide a novel post-processing approach based on a Functional Data Analysis paradigm capable of detecting precursors in displacement time series. The proposed approach appropriately accounts for the spatial and temporal dependencies of the data and does not require prior assumptions on the deformation trend. As an illustrative case, we apply the developed method to the identification of precursors to a mud volcano eruption in the Santa Barbara village in Sicily, southern Italy, showing the advantages of using a Functional Data Analysis framework for anticipating the warning signal. Indeed, the proposed approach is able to detect precursors of the paroxysmal event in the time series of the locations close to the eruption vent and provides a warning signal months before a scalar approach would. The method presented can potentially be applied to a wide range of geological events, thus representing a valuable and far-reaching monitoring tool.

Phylogenetics, character evolution, and historical biogeography of the Neotropical genus Besleria (Gesneriaceae)

Abstract Besleria, a genus of perennial herbs, shrubs, or small trees growing in the understorey of rainforests, is one of the largest genera of neotropical Gesneriaceae, with over 165 species. Despite the ecological importance and ubiquity of Besleria in rainforest ecosystems, taxonomic and evolutionary studies of Besleria are limited. Here, we generated a phylogenetic analysis of Besleria using four nuclear and chloroplast DNA regions (ITS, matK, rps16, and trnL-trnF) covering more than 50% of the recognized species, along with two secondary calibration points to infer divergence times. Our results support the monophyly of Besleria and allowed us to revise the infrageneric classification and biogeographical history of the genus. We identified five major clades that do not correspond to sections or subsections in previous classifications. These clades are well circumscribed geographically but remain difficult to characterize using previously hypothesized morphological characters. Biogeographical reconstructions indicate an origin in the northern Andes during the Middle Miocene (ca. 15 Mya). The current distribution patterns of this plant group have been significantly shaped by geological and climatic events, particularly Andean uplift and the formation of the Panama Isthmus.

Tsunami hazard evaluation of river embankment structures incorporating their vulnerability to seismic strong motion

Development of coastal areas in Japan for various land uses since the 1960s has contributed to industrial upgrades and improved the efficiency of transportation networks. However, there are concerns about the vulnerability of developments on alluvial plains and reclaimed lands to geological events, like ground subsidence due to liquefaction during large earthquakes. Realistic assessment of earthquake and tsunami hazards and evaluation of possible countermeasures require accurate estimation of the amount of subsidence that can be expected from liquefaction at coastal and riverside sites supporting various structures. In this study, to evaluate the amount a river embankment structure might be expected to settle as a result of strong motion from an assumed Nankai Trough great earthquake, we conducted a numerical simulation using the soil–water coupled finite deformation analysis code GEOASIA. We then investigated the effect of the estimated embankment subsidence on tsunami inundation, which was simulated by using nonlinear shallow-water equations and a grid spacing as fine as 3.3 m. The influence of urban structures on the inundated area was taken into account by using a structure-embedded elevation model (SEM). The results showed that subsidence of river embankments and the collapse of parapet walls on top of them would increase both the depth and area of inundation caused by a tsunami triggered by a Nankai Trough scenario earthquake. Our findings underscore the importance of evaluating not only earthquake resistance but also vulnerability of coastal and riverside structures to strong motion in tsunami hazard analyses. Furthermore, the importance of tsunami inundation analysis using a SEM for predicting the behavior of tsunami flotsam in urban areas was demonstrated.

Optimal Design of Resilient Carbon Capture, Utilization and Storage (CCUS) Supply Chain Networks under Facility Disruption

In recent years, various kinds of carbon dioxide capture, utilization and storage supply chain network design (CCUS SCND) problems have been extensively studied by scholars from the supply chain management community and other fields. The existing works mainly focus on the various deterministic or uncertainty problems; few works consider the CCUS SCND resilience problem in the context of utilization/storage facility disruptions due to unexpected natural disasters or other geological anomaly events. This paper aims to study the CCUS SCND resilience problem under utilization/storage facility capacity disruption risk. We propose a stochastic mixed-integer linear programming model for the considered problem. In the considered problem, the main decisions related to the following areas are taken into account: supply chain design and planning; facility disruption risk handling, including the optimal determination of facility locations and the matching of carbon dioxide emission sources and utilization/storage facilities; carbon dioxide normal transportation planning; and transshipment planning for various disruption scenarios. Finally, an experimental study comprising a case study from China is conducted to validate the effectiveness and performance of our proposed model. The obtained results show that the supply chain networks for the case study obtained by our proposed model are efficient, cost-effective and resilient in mitigating various kinds of utilization/storage facility disruption scenarios, showing the model can be applied to large-scale CCUS projects to help managers effectively deal with disruption risks. Future research should consider multiple disruption events and propose multiple effective resilience strategies.

Phylogeography of the freshwater crab Potamon persicum (Decapoda: Potamidae): an ancestral ring species?

The Zagros Mountains, characterized by complex topography and three large drainage systems, harbor the endemic freshwater crab Potamon persicum in Iran. Our study delves into the evolutionary history of P. persicum, utilizing two mitochondrial and one nuclear marker. We collected 214 specimens from 24 localities, identifying 21 haplotypes grouped into two major evolutionary lineages. Substantial differentiation exists between drainage systems and lineages. Historical demographic analysis revealed a significant decrease in population size during the late Holocene, accompanied by a recent population bottleneck. Species distribution modeling has revealed eastward shifts in suitable habitats between the last glacial maximum and the present day. Following the last glacial maximum, habitat fragmentation occurred, resulting in the establishment of small populations. These smaller populations are more vulnerable to climatic and geological events, thereby limiting gene flow and accelerating genetic differentiation within species. Historical biogeographic analysis traced the origin of P. persicum to the western Zagros Mountains, with major genetic divergence occurring during the Pleistocene. Our genetic analyses suggest that P. persicum may have shown a genetic pattern similar to a classical ring species before the Pleistocene. The Namak Lake sub-basin could have served as a contact zone where populations did not interbreed but were connected through gene flow in a geographic ring. Currently, genetic separation is evident between basins, indicating that P. persicum in the Zagros Mountains is not a contemporary ring species. Also, our biogeographical analysis estimated that range evolution may have been driven initially by dispersal, and only during the late Pleistocene by vicariance.

Geochemical Characteristics of Trace Elements and Mineralization Model of the Ediacaran-Early Cambrian Phosphorites, South China.

As a nonrenewable resource, phosphate rock is an important support for the development and survival of the national economy. The regional distribution and output of phosphate rock in China are extremely uneven, and the amount of high-quality ore resources is relatively poor, which seriously restricts the development and utilization of phosphate rock resources in China. This paper briefly summarizes the distribution characteristics of phosphate rock resources and summarizes the characteristics and research progress of Ediacaran-early Cambrian phosphorus mineralization types, geological characteristics, and deposit genesis of the Yangtze platform in South China. The Ediacaran-early Cambrian sedimentary phosphorite deposits in China are mainly distributed in Yunnan, Guizhou, Hubei, Sichuan and Hunan provinces of the Yangtze platform, in which the early Cambrian phosphate deposits are also rich in rare earth elements, associated with uranium, nickel, molybdenum, vanadium, and other beneficial metal elements. The increase of atmospheric oxygen content at the Ediacaran-Cambrian boundary may have promoted the extensive oxygenation of the late Neoproterozoic oceans, so the Ediacaran-early Cambrian oceans generally showed a reductive environment, and there may be dynamic chemical stratification of the oxidation zone-sulfide zone-iron zone. Up to the early Cambrian, the redox stratified structure of Precambrian seawater may still be inherited, showing that the surface water is an oxidizing environment, changing to a reduction environment, and even wedge-shaped sulfide water is developed at the bottom of the deep basin. The main phosphorus sources are deep phosphorus-rich seawater, continental weathering, and deep hydrothermal activity of Ediacaran-early Cambrian marine sedimentary phosphorite deposits in South China. The genetic mechanisms of phosphorite deposits in the Yangtze platform in South China are mainly biogenic, upwelling phosphorus-forming theory, mechanical mineralization, and syn-sedimentary hot water mixed genesis. In the future, it is still necessary to further explore the internal relationship between phosphorus deposits and major geological events, the in situ analysis of microstructure of phosphate rock ores, and the genetic mechanism of phosphate deposits and the reconstruction of paleo-marine environment.

Ancient diversity within Diporodrilus (Crassiclitellata, Annelida) clarify the historical biogeography of Corso-Sardinian earthworms

Corsica and Sardinia are amongst the largest islands of the Western Mediterranean. Their complex geological history included belonging to the European–Iberian continental margin (close to current-day Catalonia and Provence) and varying degrees of isolation for the last 30 million years, leading to peculiar, highly endemic faunas and floras. This is especially true for their earthworm faunas, which include endemic species of several Lumbricoidea genera and the endemic family (or subfamily) Diporodrilidae. Only three species have been described for the morphologically unique Diporodrilus, but there exists evidence for wide morphological variability within them and the existence of several species-level genetic lineages within Corsica. This work aimed to investigate the genetic diversity and phylogenetic relationships between the genetic lineages of Diporodrilus from Corsica and Sardinia (based on the sequences of 5 mito-nuclear markers), to perform an integrative systematics revision combining species delimitation techniques and morphological data, and to obtain a time-calibrated phylogeny of Diporodrilus and other Corso-Sardinian Lumbricoidea. Within 15 populations of the morphospecies Diporodrilus omodeoi and Diporodrilus pilosus across Corsica and Sardinia, 10 species-level genetic lineages were detected. Phylogenetic independence, high genetic divergence and morphological differences provided the support for the description of five new pseudocryptic species: Diporodrilus rotundus sp. nov., Di. jorgei sp. nov., Di. minor sp. nov., Di. meridionalis sp. nov. and Di. telti sp. nov. Time-calibrated phylogenetic inference estimated the age for genus Diporodrilus at 65.9 Mya; even if other Corso-Sardinian genera were significantly younger, all of them presented deep divergences predating the break-off of the microplate from the continent. The almost threefold increase in the known diversity of Diporodrilus stresses the need for comprehensive earthworm diversity inventories in both Sardinia and Corsica, and for studies on their ecological role and conservation status. The close correspondence between some geological and cladogenetic events suggest that the distribution of Corso-Sardinian earthworms could be used to inform standing geological controversies.

ILOSCAR: interactive Long-term Ocean-atmosphere-Sediment CArbon cycle Reservoir model v1.0

Computationally inexpensive carbon cycle models serve as critical and efficient tools for illuminating the complex dynamics of the carbon cycle and its interplay with the climate system, offering insights into how our planet has responded to climate perturbations throughout its history. During geologic hyperthermal events, carbon cycle models are employed to trace the trajectory of carbon emissions and establish a connection between the emission trajectory and changes in the Earth's surface environment. To date, the prevalent method to estimate the carbon emission rate relies on coupling the carbon cycle modeling and proxy reconstructions. Most previous studies employ a forward methodology, i.e., they force the model with an array of predefined carbon injection scenarios and select the one that produces the best fit to one or more specific proxy-derived records (e.g., atmospheric pCO2, sea surface pH, and calcite compensation depth) as the optimal solution. However, this forward method has two potential disadvantages. First, it can be computationally expensive, particularly when tens of thousands of scenarios need to be conducted to find the best solution. Second, it might not yield the best injection trajectory if none of the predefined carbon emission curves represents the realistic emission curve. Hence, an inverse model that can reconstruct the carbon emission trajectory directly from the record/records is urgently needed. In this study, building upon the Long-term Ocean-atmosphere-Sediment CArbon cycle Reservoir (LOSCAR) Model (Zeebe, 2012), we develop an interactive carbon cycle model (named iLOSCAR) using the open-source Python language and include two options, a forward model and an inverse model. The forward model replicates the original LOSCAR model, while the inverse model calculates the emission trajectory constrained by the proxy records in a single run. Both models are accessible via a web-based interface, which allows users to interactively tune model parameters and conduct experiments. In this paper, we present the details of iLOSCAR, including model structure and derivation of key equations. We then validate iLOSCAR's performance through an identical twin test and model intercomparison. We also apply it to a climatic perturbation event to diagnose features of the emission pattern that were overlooked in previous studies. Finally, we discuss the possible directions for model's future development.

The complete mitochondrial genome and phylogenetic position of Schizothorax argentatus (Cyprinomorpha: Crypriniformes: Cyprinidae)

We investigated the vulnerable fish species Schizothorax argentatus Kessler, 1874, using low-coverage whole genome sequencing data. The assembled 16,587 bp mitochondrial genome has a nucleotide composition of A = 29.8%, T = 25.3%, G = 17.9%, and C = 27.0%, containing 13 protein-coding genes, two ribosomal RNA genes, and 22 transfer RNA genes with a gene arrangement identical to other cofamilial species. Phylogenetic analyses of 71 schizothoracine fishes suggest a close relationship between S. argentatus and Schizothorax eurystomus, sharing a common ancestor with Schizothorax pseudoaksaiensis. Our study supports dividing extant schizothoracine fishes into two tribes, Schizothoracini and Schizopygopsini. The estimated time to most recent common ancestor (tMRCA ) and their distribution imply geological and climatic events during the Miocene around the Qinghai-Tibet Plateau as significant evolutionary drivers explaining the diversification of main clades in schizothoracine fishes.