ABSTRACTThe sediment samples from Cretaceous sequences, Lower Indus Basin, were investigated using biomarkers approach to predict the source, depositional environment and lithology of organic matter. The Parh and Upper Goru Formations demonstrate the presence of algal organic matter deposited under anoxic and suboxic conditions. The interpretation was supported by relatively high distribution of C27 5α (H), 14α (H), 17α (H) 20R (ααα-20R) steranes. The samples of Lower Goru Formation reveal mixed organic matter, predominantly terrestrial and oxic, as apparent from biomarkers distribution. The Sembar Formation specifies mixed input of organic matter more terrigenous input at intervals, deposited under anoxic and suboxic conditions. Geochemical analyses performed on Cretaceous strata show that hydrocarbons were derived from organic matter of mixed origin, predominantly terrestrial, deposited under anoxic and oxic conditions.