Three new species of predatory mites belonging to the genus Amblyseius Berlese (Acari: Phytoseiidae), A. latisetosus sp. nov., A. unodentatus sp. nov., and A. altaiensis sp. nov. are described based on the material collected from Altai Republic, Russia. The new species are assigned to the obtusus species group and andersoni species subgroup by having setae J2 and Z1, short and minute setae z4, sub-pentagonal ventrianal shield, and bell-shaped calyx of spermatheca. All new species were compared with each other and all other Amblyseius species known to date. The main diagnostic characters of these species are measurements of prominent dorsal setae, ventral setae JV5 and macrosetae on leg IV, cheliceral dentition, number of setae on genu II as well as small rounded and distinct pre-anal pores on ventrianal shield.