An experiment was carried out at College of Agriculture, University of Agricultural Sciences, Raichur under north eastern dry zone of Karnataka, India during late-kharif season of 2022-23 by using twenty-five tomato genotypes to study the variability, heritability and genetic advance for growth, yield and quality traits. Analysis of variance revealed that significant variability was observed among the genotypes of tomato for all character’s studied. Genetic variability revealed that a lot of variation was observed among the genotypes studied. In general, the lowest difference in phenotypic and genotypic coefficients of variation indicated lowest environmental influence in controlling the expression of the traits. Based on genetic variability studies, high PCV and GCV and high heritability coupled with high genetic advance as per cent of mean were recorded for plant height, number of nodes per plant, number of branches per plant, number of locules per fruit and titrable acidity, average fruit weight, fruit yield per plant and fruit yield per hectare indicating the existence of wider genetic variability for these traits in the germplasm under study.