Genetic Variation In Response Research Articles

The association between MDR1 C3435T genetic polymorphism and the risk of multidrug-resistant epilepsy in Egyptian children

BackgroundEpilepsy is a chronic disease affecting about 2% of the population and is considered a serious neurological disease. Despite its good prognosis, 20–30% of epileptic patients were not cured of their seizures even with the many trials of antiepileptic drug (AED) therapy. The resistance mechanism is still unclear, maybe due to the effect of the genetic factors on the bioavailability of the drugs. Consequently, the association between therapy resistance and the presence of a gene called “multidrug resistance 1 (MDR1)” had been proposed. Thus, the present study aimed to understand the relationship between the genetic polymorphism of MDR1C3435T and the resistance to AEDs.ResultA non-significant association was found between MDR1 C3435T single-nucleotide polymorphism (SNP) and drug-resistant epilepsy. However, there was statistical significance in the association between the drug type and the genotype distribution, in cases that were maintained on sodium valproate and MDR1C3435T genotype.ConclusionPossible involvement of the MDR1 gene C 3435T polymorphism with sodium valproate resistance clarifies the importance of genetic variability in response to the drug and may help to find novel genetic therapy for epilepsy, by targeting the biological mechanisms responsible for epilepsy in each specific individual. Future studies with bigger sample sizes and in other racial populations will be necessary.

Yield and photosynthetic rate of wheat under continuously high temperature

In tropical regions high temperature occurs throughout wheat growth cycle and is a major factor influencing growth and yield in this area. The aim of this work is to describe the responses to continuously-high temperatures on growth, photosynthesis and yield of wheat, under controlled conditions. Nias and Dewata (Indonesian wheat varieties) and Axe and Gladius (Australian varieties) were tested in growth room experiment with temperature and photoperiod chosen to simulate conditions on Lombok Island, at lowland (32/23°C) and highland (28/20°C) sites. The third temperature (25/15°C) was selected to represent temperature in a more temperate wheat-producing area. High temperature reduced yield and dry matter accumulation which was associated with a reduction in photosynthetic rate and stomata conductance and an increase in respiration rate. The reduction in photosynthetic rate at high temperature was not only due to lower stomatal conductance but also non-stomatal effects as mesophyll conductance and quantum yield were lower. Genetic variability in response to heat stress was evident with the Indonesian varieties being more tolerant to high temperatures than Australian varieties. Nias and Dewata produced higher yield and biomass and maintaining higher rates of photosynthesis. Maintaining high photosynthetic rate and high stomata conductance, are important characters in adapting wheat into tropical environment such as Lombok Island.

Adaptation of advanced clinical virology assays from HIV-1 to SARS-CoV-2.

Purpose of reviewIn response to the HIV–AIDS pandemic, great strides have been made in developing molecular methods that accurately quantify nucleic acid products of HIV-1 at different stages of viral replication and to assess HIV-1 sequence diversity and its effect on susceptibility to small molecule inhibitors and neutralizing antibodies. Here, we review how knowledge gained from these approaches, including viral RNA quantification and sequence analyses, have been rapidly applied to study SARS-CoV-2 and the COVID-19 pandemic.Recent findingsRecent studies have shown detection of SARS-CoV-2 RNA in blood of infected individuals by reverse transcriptase PCR (RT-PCR); and, as in HIV-1 infection, there is growing evidence that the level of viral RNA in plasma may be related to COVID disease severity. Unlike HIV-1, SARS-CoV-2 sequences are highly conserved limiting SARS-CoV-2 sequencing applications to investigating interpatient genetic diversity for phylogenetic analysis. Sensitive sequencing technologies, originally developed for HIV-1, will be needed to investigate intrapatient SARS-CoV-2 genetic variation in response to antiviral therapeutics and vaccines.SummaryMethods used for HIV-1 have been rapidly applied to SARS-CoV-2/COVID-19 to understand pathogenesis and prognosis. Further application of such methods should improve precision of therapy and outcome.

The genetic variation in response to drought in Tunisian populations of Brachypodium hybridum (Poaceae): an interplay between natural selection and phenotypic plasticity

With the ongoing climate change, widespread changes could be noticed in both the extent of the geographical distribution as well the patterns of phenotypic variation of plant species in response to environmental heterogeneity. Whether these changes are a direct consequence of global climate change, and whether they result from phenotypic plasticity or evolutionary forces, are currently main areas of interest. In the present study, 130 lines belonging to ten Tunisian Brachypodium hybridum populations differing greatly in climatic conditions were exposed to water stress and the pattern of their genetic variation was analyzed using sixteen functional traits in order to unravel the main drivers of their response to drought. Our results showed that B. hybridum populations exhibited an extensive intra-population genetic variation in control and water stress, whereas principal component analysis did not show any clear populations grouping pattern. In addition, ANOVA showed that most of analyzed traits revealed a significant population and line effects, indicating that their response to drought was, in part, controlled at the genetic level. These results suggest that the natural selection on standing intra-population genetic variation plays a major role in dealing with drought in B. hybridum. On the other hand, we found that many traits exhibited phenotypic plasticity in response to water stress. The plasticity index showed that the populations responded differently to water stress, with Ain Drahem and Djerba populations showed the highest and the lowest plasticity levels, respectively. However, plasticity of most analyzed traits seems to be unrelated to climatic factors, except for the precipitation of wettest month, which showed significant correlation with five traits related to biomass production and the mean plasticity index. In summary, the present study revealed that the genetic variation in response to drought in the natural populations of B. hybridum was most likely governed by an interplay between two major evolutionary and ecological processes: natural selection and phenotypic plasticity.

Scion genotypes exert long distance control over rootstock transcriptome responses to low phosphate in grafted grapevine

BackgroundGrafting is widely used in horticulture and rootstocks are known to modify scion growth and adaptation to soil conditions. However, the role of scion genotype in regulating rootstock development and functioning has remained largely unexplored. In this study, reciprocal grafts of two grapevine genotypes were produced as well as the corresponding homo-graft controls. These plants were subjected to a low phosphate (LP) treatment and transcriptome profiling by RNA sequencing was done on root samples collected 27 h after the onset of the LP treatment.ResultsA set of transcripts responsive to the LP treatment in all scion/rootstock combinations was identified. Gene expression patterns associated with genetic variation in response to LP were identified by comparing the response of the two homo-grafts. In addition, the scion was shown to modify root transcriptome responses to LP in a rootstock dependent manner. A weighted gene co-expression network analysis identified modules of correlated genes; the analysis of the association of these modules with the phosphate treatment, and the scion and rootstock genotype identified potential hub genes.ConclusionsThis study provides insights into the response of grafted grapevine to phosphate supply and identifies potential shoot-to-root signals that could vary between different grapevine genotypes.

Genetic Variation in Response to Global Warming in a Coral Reef Species, Porites lobata

Climate change due to global warming is one of the worst environmental disasters in the world, which affects all ecosystems and has led to increasing degradation of coral reefs. The increase of sea surface temperature is inversely related to the resistance of corals and is directly associated with their bleaching. High temperature disrupts the symbiotic relationship between coral and algal symbiont and results in coral bleaching. To evaluate the adaptation of corals to heat stress, in this study, we investigated the thermal stress effect on the expression of genes involved in programmed cell death (PCD), cysteinyl aspartate proteases 3 (will be mentioned as Caspas3 hereafter) and anti-apoptotic pathway, B-cell lymphoma 2 (will be mentioned as Bcl2 hereafter) in Porites lobata (Dana, 1846). Corals were incubated at 25°C for 2 weeks (adaptation period) and then exposed to 34°C (heat shock) for 24 and 48 hours. Then, the expression of genes was measured using real-time PCR. The results revealed that both genes were up-regulated at 24 hours after heat induction. Bcl-2 expression (anti-apoptotic gene) was induced at 24 hours and was down-regulated at 48 hours. In contrast, Caspase3 (apoptotic gene) continued to be expressed up to 48 hours. These results might indicate that coral cells are headed towards bleaching and death with increased temperature. The results of this study, regarding the observed expression patterns, can clarify the response of different genes to a thermal stress in coral reefs. The exposure of corals to acute conditions with high temperatures presented the behavior of the desired genes in the studied conditions.

Proteomics, physiological, and biochemical analysis of cross tolerance mechanisms in response to heat and water stresses in soybean.

Water stress (WS) and heat stress (HS) have a negative effect on soybean plant growth and crop productivity. Changes in the physiological characteristics, proteome, and specific metabolites investigated on molecular and cellular functions were studied in two soybean cultivars exposed to different heat and water stress conditions independently and in combination. Leaf protein composition was studied using 2-DE and complemented with MALDI TOF mass spectrometry. While the two cultivars displayed genetic variation in response to water and heat stress, thirty-nine proteins were significantly altered in their relative abundance in response to WS, HS and combined WS+HS in both cultivars. A majority of these proteins were involved in metabolism, response to heat and photosynthesis showing significant cross-tolerance mechanisms. This study revealed that MED37C, a probable mediator of RNA polymerase transcription II protein, has potential interacting partners in Arabidopsis and signified the marked impact of this on the PI-471938 cultivar. Elevated activities in antioxidant enzymes indicate that the PI-471938 cultivar can restore the oxidation levels and sustain the plant during the stress. The discovery of this plant's development of cross-stress tolerance could be used as a guide to foster ongoing genetic modifications in stress tolerance.

Pharmacogenomics - the state of the art in drug prescription

Abstract Introduction The different response to various drugs by individuals has been increasingly debated, since it has been concluded that there are large differences in response and increasing occurrence of adverse reactions. This set of issues led to more and more research on the implementation of genetic testing in the very near future, leading to benefits for the patient. The purpose of pharmacogenetics is to analyse each individual’s genetic variability in response to therapy, increasing efficiency and safety. On the other hand, pharmacogenomics studies how the expression of a set of genes interferes in individual’s responses to drugs, taking into account pharmacokinetics and pharmacodynamics. This concept also seeks to reduce adverse reactions and toxicity, maximizing drug efficiency. Objectives The present work aims at gathering information on the proposed theme, making the survey of drugs available on the market elucidating how it allows the adjustment of the dose to be administered to the patient according to their genetic profile. Methodology Databases such as Pubmed, Science Direct and Google Scholar were consulted and data were collected from 2015 until 2019. Paper was selected first by abstract and after by full text reading of the article. Results On this review we will list the drugs associated with each biomarker and the respective therapeutic area to which they belong, compiled by the Food and Drug Administration (FDA). This list includes the main therapeutic area: oncology, haematology, anaesthesiology and psychology. Biomarkers allow us to identify phenotype-associated variations in drug response, making it possible to understand whether drugs will have a beneficial effect, no effect or if there is a risk of toxicity. Conclusion This question requires a risk-benefit assessment, since it involves social, ethical and economic problems.

Adaptive genetic variation to drought in a widely distributed conifer suggests a potential for increasing forest resilience in a drying climate.

Summary Drought intensity and frequency are increasing under global warming, with soil water availability now being a major factor limiting tree growth in circumboreal forests. Still, the adaptive capacity of trees in the face of future climatic regimes remains poorly documented.Using 1481 annually resolved tree‐ring series from 29‐yr‐old trees, we evaluated the drought sensitivity of 43 white spruce (Picea glauca (Moench) Voss) populations established in a common garden experiment.We show that genetic variation among populations in response to drought plays a significant role in growth resilience. Local genetic adaptation allowed populations from drier geographical origins to grow better, as indicated by higher resilience to extreme drought events, compared with populations from more humid geographical origins. The substantial genetic variation found for growth resilience highlights the possibility of selecting for drought resilience in boreal conifers.As a major research outcome, we showed that adaptive genetic variation in response to changing local conditions can shape drought vulnerability at the intraspecific level. Our findings have wide implications for forest ecosystem management and conservation.

Comparing Salt Tolerance at Seedling and Germination Stages in Local Populations of Medicago ciliaris L. to Medicago intertexta L. and Medicago scutellata L.

Salt stress is one of the most serious environmental stressors that affect productivity of salt-sensitive crops. Medicago ciliaris is an annual legume whose adaptation to agroclimatic conditions has not been well described. This study focused on the salinity tolerance of M. ciliaris genotypes compared to M. intertexta and M. scutellata in terms of plant growth, physiology, and biochemistry. Salt tolerance was determined at both germination and early seedling growth. Germination and hydroponic assays were used with exposing seeds to 0, 50, 100, 150, and 200 mM NaCl. Among seven genotypes of M. ciliaris studied, Pop1, 355, and 667, were most salt tolerant. Populations like 355 and 667 showed marked tolerance to salinity at both germination and seedling stages (TI ≤1, SI(FGP) > 0 increased FGP ≥ 20% and SI(DW) < 0 (DW decline ≤ 20%); at 100 mM); while Pop1 was the most salt tolerant one at seedling stages with (TI =1.79, SI(FGP) < 0 decline of FGP ≤ 40% and with increased DW to 79%); at 150 mM NaCl). The genotypes, 306, 773, and M. scutellata, were moderately tolerant to salt stress depending on salt concentration. Our study may be used as an efficient strategy to reveal genetic variation in response to salt stress. This approach allows selection for desirable traits, enabling more efficient applications in breeding methods to achieve stress-tolerant M. ciliaris populations.

The effects of continuously high temperatures on apical development and yield of four varieties of wheat

The aim of the experiment was to examine how wheat responded to an extended period of high temperatures under controlled conditions to supplement field studies with these varieties on Lombok Island. Two Indonesian wheat varieties (Nias and Dewata) and two Australian varieties (Axe and Gladius) were examined in growth room experiments at 3 different temperature regimes 32/23°C, 28/20°C and 25/15°C day/night with 12 h daylight. Temperature and photoperiod were selected to simulate conditions on Lombok Island, at lowland (32/23°C) and highland (28/20°C) sites. A third temperature (25/15°C) was selected to represent the temperature in a more temperate wheat producing area. The rate of plant development increases with the rise of the temperatures up to an optimum temperature and slower after further increases. Despite being exposed to high temperatures from the establishment, the effect of high temperature was more severe during the reproductive stage as seen by the fact that yield was more affected than dry matter accumulation and yield was most strongly related to grain number. Genetic variability in response to heat stress was evident with the Indonesian varieties being more tolerant to high temperatures than Australian varieties. Nias and Dewataproduced higher yield and biomass.

Genetic Variation in Response to N, P, or K Deprivation in Baby Leaf Lettuce

Lettuce harvested at the baby leaf stage is a popular component of mixed salads in ready-to-use packages. Little is known, however, about response of baby leaf lettuce to nitrogen (N), phosphorus (P), and potassium (K) fertilization. Eight lettuce accessions were subjected to five fertilization treatments to investigate genetic differences in reaction to N, P, and K fertilization. The control treatment provided optimal levels of macronutrients for plant growth, while other treatments deprived plants of either N, P, or K. Potassium deprivation had no obvious effect on plant weight or composition, apart from substantially decreased potassium content. Nitrogen and phosphorus deprivations, however, extensively decreased fresh weight and affected plant composition. Phosphorus and nitrogen deprivation considerably increased anthocyanin content in red-colored accessions, but anthocyanin was decreased in dark green-colored accessions, indicating differences in regulation of anthocyanin biosynthesis. Correlations between fresh weight, chlorophyll, anthocyanin, nitrogen, phosphorus, and potassium content were substantially affected by selection of datasets used for analyses; some relationships were revealed when analyzed separately by individual treatments, while others were more likely to be detected when analyzed by individual accessions. Absolute (ΔABS) and relative (2ΔREL) parameters described in this study were suitable for detecting over- and underperforming accessions. The ΔABS identified the absolute Lb-fold (logarithm to the base of 2, binary logarithm) change in performance of an accession in a treatment as compared to its performance in control conditions. The 2ΔREL parameter showed relative Lb-fold change for an accession as compared to the overall mean of ΔABS values of all accessions tested in control and treatment conditions.

Phenotypic traits of Burkholderia spp. associated with ecological adaptation and plant-host interaction

Burkholderia species have different lifestyles establishing mutualist or pathogenic associations with plants and animals. Changes in the ecological behavior of these bacteria may depend on genetic variations in response to niche adaptation. Here, we studied 15 Burkholderia strains isolated from different environments with respect to genetic and phenotypic traits. By Multilocus Sequence Analysis (MLSA) these isolates fell into 6 distinct groups. MLSA clusters did not correlate with strain antibiotic sensitivity, but with the bacterial ability to produce antimicrobial compounds and control orchid necrosis. Further, the B. seminalis strain TC3.4.2R3, a mutualistic bacterium, was inoculated into orchid plants and the interaction with the host was evaluated by analyzing the plant response and the bacterial oxidative stress response in planta. TC3.4.2R3 responded to plant colonization by increasing its own growth rate and by differential gene regulation upon oxidative stress caused by the plant, while reducing the plant's membrane lipid peroxidation. The bacterial responses to oxidative stress were recapitulated by bacterial exposure to the herbicide paraquat. We suggest that the ability of Burkholderia species to successfully establish in the rhizosphere correlates with genetic variation, whereas traits associated with antibiotic resistance are more likely to be categorized as strain specific.

Species-specific genetic variation in response to deep-sea environmental variation amongst Vulnerable Marine Ecosystem indicator taxa

Understanding the ecological processes that shape spatial genetic patterns of population structure is critical for understanding evolutionary dynamics and defining significant evolutionary and management units in the deep sea. Here, the role of environmental factors (topographic, physico-chemical and biological) in shaping the population genetic structure of four deep-sea habitat-forming species (one sponge - Poecillastra laminaris, three corals - Goniocorella dumosa, Madrepora oculata, Solenosmilia variabilis) was investigated using seascape genetics. Genetic data (nuclear and mitochondrial sequences and microsatellite multilocus genotypes) and environmental variables were employed to build individual-based and population-level models. The results indicated that environmental factors affected genetic variation differently amongst the species, as well as at different geographic scales. For individual-based analyses, different environmental variables explained genetic variation in P. laminaris (dissolved oxygen), G. dumosa (dynamic topography), M. oculata (sea surface temperature and surface water primary productivity), and S. variabilis (tidal current speed). At the population level, factors related to current and food source explained the regional genetic structure in all four species, whilst at the geomorphic features level, factors related to food source and topography were most important. Environmental variation in these parameters may be acting as barriers to gene flow at different scales. This study highlights the utility of seascape genetic studies to better understand the processes shaping the genetic structure of organisms, and to identify environmental factors that can be used to locate sites for the protection of deep-sea Vulnerable Marine Ecosystems.

Translational regulation contributes to the elevated CO2 response in two Solanum species.

SummaryUnderstanding the impact of elevated CO2 (eCO2) in global agriculture is important given climate change projections. Breeding climate‐resilient crops depends on genetic variation within naturally varying populations. The effect of genetic variation in response to eCO2 is poorly understood, especially in crop species. We describe the different ways in which Solanum lycopersicum and its wild relative S. pennellii respond to eCO2, from cell anatomy, to the transcriptome, and metabolome. We further validate the importance of translational regulation as a potential mechanism for plants to adaptively respond to rising levels of atmospheric CO2.

Genetic diversity using single nucleotide polymorphisms (SNPs) and screening for salinity tolerance in rice germplasm at reproductive stage

AbstractGenetic diversity is a valuable asset for crop improvement. In this study, a total of 50 rice genotypes were screened for salinity tolerance at the reproductive stage using gravel-based hydroponics, soil, controlled mini-field and field methods. Different morpho-agronomic, physiological markers and tolerance indices were used to classify tolerant and susceptible genotypes. Our results showed high genetic variability in response of rice genotypes to salinity using different screening methods. The significant effect (P &lt; 0.01) of salinity include increased Na+ sequestration in the flag leaf, increased unfilled grains (except for the field method) and reduced pollen fertility, total yield, panicle length and the average number of filled grains per panicle. Plant height (except for the soil method) and K+ ion concentration in the flag leaf were not significantly affected by salinity (P &gt; 0.05). Genetic diversity analysis indicated that the germplasm evaluated exhibits moderate diversity (PIC ${\overline{\bf X}}$ = 0.2085). Cluster analysis using single nucleotide polymorphisms showed that the genetic make-up of rice germplasm somehow did not necessarily indicate their over-all tolerance or susceptibility to salinity. This study proved that the controlled mini-field method is the most advantageous among the screening methods while geometric mean productivity, stress tolerance index and yield index are the tolerance indices that can be classified as better predictors of salinity tolerance considering the yield potentials of the genotypes. The genotypes Nona Bokra and Mushkan 41 can be used for breeding in the future through low Na+:K+ ratio while Damodar and Bhura Rata 4-10 for breeding salt-tolerant cultivars with higher yield potentials.

Modulation in growth, biochemical attributes and proteome profile of rice cultivars under salt stress

One of the major abiotic stresses that affect productivity of rice is salinity. Rice cultivars showed significant genetic variation in response to salt stress. In the present investigation, differential growth pattern and physio-chemical traits-based screening of high yielding rice cultivars of various salt affected areas of India was carried out, and salt-sensitive and salt-tolerant cultivars were identified. Differential responses of antioxidant enzyme activity and tolerance index at maximum level of salt treatment depicted that Jhelum and Vytilla-4 cultivars of rice were sensitive and tolerant to salt stress, respectively. Analysis of growth, morpho-physiological, and biochemical parameters also confirmed the salt-tolerant and salt-sensitive characters of cv. Vytilla-4 and cv. Jhelum, respectively. Nano-LCMS/MS-based proteome profile of these two cultivars was carried out to find out the mechanism lying behind the salt tolerance. A total number of 514 and 770 protein spots were reported in the most salt-tolerant (cv. Vytilla-4) and salt-sensitive (cv. Jhelum) cultivars, respectively. The differentially expressed proteins (DEPs) were found associated with major metabolic pathways including photosynthesis, energy metabolism, amino acid metabolism, nitrogen assimilation and stress and signalling pathways. The changes in the major proteins like Ribulose bisphosphate carboxylase small chain, chlorophyll a-b binding protein, phosphoglycerate kinase, cytochrome c oxidase subunit 5C, glutamine synthetase, glutathione S-transferase, peroxidase, and thioredoxin elucidated the mechanism activated by salt-tolerant cv. Vytilla-4. The transcriptional validation of some of the differentially expressed proteins through real-time quantitative PCR analysis further validated the proteomic results. Outcomes of this work could help in finding out the potential cross-links of different pathways involved in salt-tolerance mechanisms operating in the studied here rice cultivars under salt stress.

A pre‐adaptive approach for tropical forest restoration during climate change using naturally occurring genetic variation in response to water limitation

Effective reforestation of degraded tropical forests depends on selecting planting material suited to the stressful environments typical at restoration sites that can be exacerbated by increased duration and intensity of dry spells expected with climate change. While reforestation efforts in nontropical systems are incorporating drought‐adapted genotypes into restoration programs to cope with drier conditions, such approaches have not been tested or implemented in tropical forests. As the first effort to examine genetic variation in plant response to drought in a tropical wet forest, we established a watering experiment using five replicated maternal lines (i.e. seedlings from different maternal trees) of five dipterocarp species native to Borneo. Apart from the expected species level variation in growth and herbivory (3‐fold variation in both cases), we also found intraspecific variation so that growth in some cases varied 2‐fold, and herbivory 3‐fold, among genetically different maternal lines. In two species we found that among‐maternal line variation in growth rate was negatively correlated with tolerance to water limitation, that is, the maternal lines that performed the best in the high water treatment lost proportionally more of their growth during water limitation. We argue that selection for tolerance to future drier conditions is not only likely to impact population genetics of entire forests, but likely extends from forest trees to the communities of canopy arthropods associated with these trees. In tropical reforestation efforts where increased drought is predicted from climate change, including plant material resilient to drier conditions may improve restoration effectiveness.

Soybean intraspecific genetic variation in response to elevated CO2

ABSTRACTAlthough elevated atmospheric CO2 concentration (elevated CO2 (eCO2)) considerably affect soybean yield, the agronomic and physiological contributors to the yield variation among cultivars in response to eCO2 have not been fully understood. Such investigation will fundamentally benefit future breeding strategies to enhance soybean adaptability to eCO2 environments. Twenty-four soybean cultivars were exposed to ambient CO2 (aCO2, 390 ppm) or eCO2 (580 ppm) throughout growth stages. The results showed the stimulation of eCO2 on soybean yield varied among cultivars, resulting in the increase from 3.3% in Yuanbaojin to 95% in Suinong 8. The yield gain under eCO2 was negatively correlated with yield under aCO2. The increases of the pod and seed number (sink) were strongly positive correlated (P< 0.001) with the increase of yield, but not the change of seed number per pod, seed size, leaf area, and leaf nitrogen and chlorophyll concentrations at the initial pod filling stage (R5). There was a strong positive correlation between yield gain and the accumulation of biomass during R5–R8 (full maturity). These results indicated that, among cultivars, the potential of sink size and the strength of photosynthetic capability during R5–R8 could be the limiting factors to yield response to eCO2.

Personalised nutrition - phenotypic and genetic variation in response to dietary intervention.

Personalised nutrition allows individual differences in dietary, lifestyle, anthropometry, phenotype and/or genomic profile to be used to direct specific dietary advice. For personalised nutrition advice to be effective both sides need to be considered; firstly, that factors influencing variation in response to dietary intervention are identified and appropriate advice can be derived and secondly; that these are then used effectively in the provision of nutrition advice, resulting in a positive dietary and/or lifestyle behaviour change. There is considerable evidence demonstrating genetic and phenotypic influence on the biological response to the consumption of nutrients and bioactives. However, findings are often mixed, with studies often investigating at the level of a single nutrient/bioactive and/or a single genetic/phenotypic variation, meaning the derivation of specific advice at a dietary level in an individual/group of individuals can be complex. Similarly, the impact of using this information to derive personalised advice is also mixed, with some studies demonstrating no effectiveness and others showing a significant impact. The present paper will outline examples of phenotypic and genetic variation influencing response to nutritional interventions, and will consider how they could be used in the provision of personalised nutrition.