Genetic Quality Research Articles

The resistance of the synthetic population of Indonesian common carp (Cyprinus carpio) to Koi Herpesvirus (KHV)

One of the effects of a decrease in the genetic quality of common carp is a decrease in resistance to disease. Establishing a synthetic population, blended from 5 strains, namely Rajadanu, Majalaya, Sutisna, Wildan, and Sinyonya, is expected to increase the disease resistance in the Indonesian common carp population. This study aims to evaluate the resistance of this synthetic population to Koi Herpesvirus (KHV) disease. The three common carp strains, Majalaya carp, Subang carp, and Cangkringan carp, were challenged with a cohabitation approach compared to the synthetic population. The water temperature was adjusted to the optimum condition for KHV development, which ranged from 20 – 22 °C. The result showed that KHV infection started on day three and peaked on days 6 to 10 after cohabitation. At the end of the challenge test, the survival rate of the synthetic population was 62.0 %, significantly higher than other strains (P<0.05), which ranged from 20.0 to 26.7 % of the constituent populations. The results of this challenge test indicated that the synthetic population of common carp had better resistance to KHV infection than other strains of common carp cultured by farmers.

Caracterização morfológica de novos clones-elites de bananeira

Abstract The characterization of clones is a very important step in genetic certification programs, as it describes and recognizes the plant material at every stage of production, allowing the monitoring of genetic quality, improvement and conservation of the germplasm. The aim of this work was to morphologically characterize two elite banana clones. The experimental design applied was in randomized blocks, with four replications, with six plants per parcel. The treatments consisted of two elite banana clones, Prata Gorutuba R1 and Prata Gorutuba R2 and four commercial cultivars: Prata Gorutuba, Prata Ana, Grande Naine and BRS Princesa. The morphological characterization was carried out in the second production cycle (daughter plant), using 23 qualitative descriptors, visually evaluated, classified according to the instructions for carrying out the distinguishability, homogeneity and stability (DHS) tests of banana cultivars. The elite clones Prata Gorutuba R1 and R2 differed from the cultivars Grande Naine and BRS Princesa regarding the descriptors tapering of the pseudostem, predominant color of the pseudostem, intensity of anthocyanin coloration and color of the underside of the basal sheath. The descriptors made it possible to group elite clones into the Prata group, a group that predominates in crops in Brazil, with characteristics already widely accepted by the market.

Performance and breeding value of male buffalo production traits as a basis for selection in Jembrana Regency, Bali Province, Indonesia

Efforts to increase the buffalo population must also be balanced with efforts to improve their genetic quality, so that there are many superior breeds available as a substitute for livestock that are no longer productive. The aims of this study were to determine the performance of the production traits of the young bull population and to determine the breeding value of the production traits of the young bull in Jembrana District, Bali Province, Indonesia. The data were analyzed descriptively to obtain the average production traits of young bull, and to calculate the breeding value of the measured production traits. The results showed that the average production characteristics obtained in this study were higher than the standards set by SNI 7706.1:2011, regarding young bull of mud buffalo breeds, for the minimum requirements for quantitative body dimensions (chest girth, shoulder height and body length), as a candidate for sire, especially for age group I-1. The highest breeding value was owned by young bull with code 12 and code 9.The conclusions of the study were: (i) the performance of young bull in Jembrana Regency, Bali Province was very good, because it was higher than the quantitative minimum requirements stipulated by SNI 7706.1: 2011, concerning young bull buffalo breeds and (ii) the highest breeding values ​​are owned by young bulls with code 12 and code 9, so that these two young bull are the best candidate as a sire.

Intraspecific Morphological Variations among the Populations of Milicia excelsa, Pouteria adolfi-friedericii, and Prunus africana in Different Natural Forests of Southwest Ethiopia

Plants have the ability to change their morphological and physiological traits in response to environmental variations. The objective of this study was to determine the intraspecific morphological variations among the populations of M. excelsa, P. adolfi-friedericii, and P. africana in southwest Ethiopia. Representative forests were systematically selected, and a total of ten transects of 160 m length were randomly laid at 100 m intervals, and 30 quadrats (20 m by 20 m) were laid along each transect line at 50 m intervals. Stem height, DBH, and bole length of trees for each species were measured in each quadrat. The intraspecific morphological variations among populations of each species were computed using hierarchical clustering and principal component analysis (PCA) with R.4.1.3. A total of 55 trees for M. excelsa in four forests, 232 trees for P. adolfi-friedericii in eight forests, and 184 trees for P. africana in five forests were measured. Accordingly, three, five, and three population clusters were identified for M. excelsa, P. adolfi-friedericii, and P. africana, respectively. The analysis of similarity (ANOSIM) indicated the presence of considerable dissimilarity among population clusters for M. excelsa and P. africana but was not significant at p ≤ 0.05 (R = 0.9, p = 0.17 ). However, ANOSIM indicated the presence of considerable dissimilarity among population clusters of P. adolfi-freidericii, which was significant at p ≤ 0.05 (R = 0.9, p = 0.03 ). Overall, there was a visible morphological variability among the populations of M. excelsa, P. adolfi-friedericii, and P. africana each at the different sites. Therefore, it is important to look for conservation strategies, such as domestication, to maintain and improve the variability and genetic quality among the populations in a wider scale of the ecological and social environment.

Voluntary Selection; Bringing Evolution at the Service of Humanity

Human populations can be characterized by their corresponding phenotypic profiles which do not necessarily align. These differences are not our points of strength in building harmonious societies and establishing a sustainable world order; it is therefore desirable to seek a pathway to resolve them. This article reflects on genetics as the most profound source of our differences and introduces Voluntary Selection as a means of acquiring genetic qualities and resolving the imbalance in their possession by different populations. Voluntary Selection is a healthcare program allowing for voluntarily involving the reproductive cells of another individual than the parents to conceive a child at the discretion of a parent population. Genetic qualities in this context are desired phenotypes as perceived by each population. The article then presents a computational approach through which thoughtful decisions about the design of a program can be made. These methods are applied to a pedigree data set of adult heights and the results are used to demonstrate the influence of the program design and the significance of its outcomes.

Electrical parameters of high-temperature electrical conductivity, frequency characteristics of active electrical resistance and dielectric losses of samples of minerals, rocks, ores as a function of their composition and genesis

Relevance is determined by the search and selection of the most information-intensive characteristics and physical properties of minerals and rocks for the reconstruction of genesis conditions, as well as the need for scientifically based criteria for the detection of various minerals, their quality assessment, which is especially important in selective mining and the choice of the optimal ore dressing technology. Research methodology. Для исследования электрических свойств были использованы образцы в форме кубика с ребром 0,02 м. To study the electrical properties, samples in the form of a cube with an edge of 0.02 m were used. The measurements were performed in an open system at atmospheric pressure. The electrical resistance was measured with two-electrode setup every 10 degrees in the temperature range of 20–900 ºC. The heating rate is 0.066 deg/s. Measuring instrument – E6-13 teraohmmeter with dynamic range from 10 to 1014 Ohm and relative measurement error from ±2.5 to 4% at the end of the range. The frequency dispersion of the active resistance and the dielectric loss tangent was studied at room temperature in the range of 0.01–100 kHz. The measuring device is the LCR-819 immitance meter. The technique is described in the instruction manual for the device (LCR-819 immittance meter. Operation manual. M., 2005. 26 p.). Results. Physical, physicochemical, mineralogical and petrographic methods have been used to study samples of some minerals, rocks, ores from different deposits of the Urals. Functional relationships are revealed between the electrical parameters of high-temperature electrical conductivity (activation energy E0 , the so-called electrical resistance coefficient lg R0 ), frequency characteristics of active resistance, dielectric loss tangent of the studied samples of minerals, rocks, ores with their genetic features, mineral composition and quality. The materials presented in the work are the result of many years of research by the author. Conclusions. The given results in combination with other physicochemical parameters can be used as a reliable indicator of the rapid assessment of the studied material.

The use of superior common carp for a better aquaculture

Fish farming is an economic business activity that has been carried out for a long time in Indonesia. One of the most popular commodities cultured is common carp. In 2001, common carp production was 418,910 MT, equivalent to 63.5% of Indonesia’s national production. However, the existence of obstacles in common carp culture, especially the outbreak of KHV in 2002 and the decrease of the genetic quality of fish, indicated by the slower growth of fish, resulted in a significant decrease in common carp production. The use of lower genetic quality fish causes the efficiency of the feed used is getting lower, as indicated by the high value of the FCR. In-efficient use of feed has an impact on increasing feed waste in the cultural environment. To increase common carp production, several varieties of superior common carp have been released for the farmer. Empirically, superior fish carp have a better growth rate and are more efficient in the use of feed. Culture of these superior varieties impacted to increase in the national production of common carp, by an average of 8.7%/year. On the other hand, the culture environment is kept clean due to up to 47.0±4.8% reduction in waste.

Yield Characteristics and Stability of Sugarcane Mutant in Three-Location Trials

Bululawang (BL) has been cultivated annually under the increasing environmental pressure caused by global climate change. This condition often decreases the genetic ability and yields of agricultural products. Meanwhile, point 2.4 SDGs, specifically in sugarcane, can be achieved through a breeding program to improve and increase the genetic quality of several varieties of the plant in Indonesia. Mutation breeding to produce superior species has also been carried out by the Plant Breeding Laboratory, University of Jember, and this helped to increase the national sugarcane production. Therefore, this study aims to obtain new high-yielding varieties using the BL as the parent plant. The mutation breeding was carried out using the EMS mutagen, and this led to the production of three selected genotypes, namely M1.4, M2.4, and M3.4, which were further developed and tested for multilocation. The three selected locations for this study include Sukorambi-Jember, Arjasa-Jember, and Pakem-Bondowoso Districts. Based on the LSI test, M1.4, M2.4, and M3.4 showed the highest agronomic performance and yield in all locations compared to BL. M1.4 and M3.4 were identified to have high yield stability in the multi-location trials, hence, they were recommended as new stable superior genotypes. Therefore, M3.4 was declared as a site-specific adaptive species, which can be planted in the Arjasa and Sukorambi areas, based on the stability tests of Finlay and Wikinson, Eberhart, Russell, and AMMI.

Measuring quality and value in genetic counseling: The current landscape and future directions.

Genetic counselors strive to provide high-quality genetic services. To do so, it is essential to define quality in genetic counseling and identify opportunities for improvement. This Professional Issues article provides an overview of the evaluation of healthcare quality in genetic counseling. The National Society of Genetic Counselors' Research, Quality, and Outcomes Committee partnered with Discern Health, a value-based healthcare policy consulting firm, to develop a care continuum model of genetic counseling. Using the proposed model, currently available quality measures relevant to genetic counseling in the US healthcare system were assessed, allowing for the identification of gaps and priority areas for further development. A total of 560 quality measures were identified that can be applied to various aspects of the care continuum model across a range of clinical specialty areas in genetic counseling, although few measures were specific to genetic counseling or genetic conditions. Areas where quality measures were lacking included: attitudes toward genetic testing, family communication, stigma, and issues of justice, equity, diversity, and inclusion. We discuss these findings and other strategies for an evidence-based approach to quality in genetic counseling. Strategic directions for the genetic counseling profession should include a consolidated approach to research on quality and value of genetic counseling, development of quality metrics and patient-experience measures, and engagement with other improvement activities. These strategies will allow for benchmarking, performance improvement, and future implementation in accountability programs which will strengthen genetic counseling as a profession that provides evidence-based high-quality care to all patients.

Non-Mammalian Eukaryotic Expression Systems Yeast and Fungi in the Production of Biologics.

Biologics have become an important area of medical research generating therapeutics essential for the treatment of many disease states. Biologics are defined as biologically active compounds manufactured by living cells or through biological processes termed bioprocessing. Compared to small molecules which are chemically synthesised they are relatively complex and therapeutically specific molecules. Biologics include hormones, vaccines, blood products, monoclonal antibodies, recombinant therapeutic proteins, enzymes, gene and cellular therapies amongst others. For biologic production prokaryotic and eukaryotic cells (mammalian and non-mammalian) are used as expression systems. Eukaryotic expression systems offer many advantages over prokaryotic based systems. The manufacture of high-quality proteins for human clinical use via recombinant technologies has been achieved in yeast and filamentous fungal systems. Advances in bioprocessing such as genetic engineering, bioreactor design, continuous processing, and quality by design has allowed for increased productivity and higher yield in in these non-mammalian eukaryotic systems with protein translation similar to mammalian systems. The application of eukaryotic expressions systems for the manufacture of biologics of therapeutic importance are described herein.

Identification of spermatogenesis-related lncRNA in Holstein bull testis after sexual maturity based on transcriptome analysis

Under the large-scale breeding model, the performance of the Holstein bull is directly related to the economic efficiency of the whole dairy farm and is the key factor affecting the genetic quality of the herd. Although the number of reported studies on the association of long noncoding RNAs (lncRNAs) with male reproduction is increasing, there is a lack of research on how lncRNAs regulate Holstein bull testicular development and spermatogenesis. To explore the molecular mechanisms between lncRNAs and spermatogenesis, three 8-week-old Holstein bull (young bull, YB) testes and three 80-week-old Holstein bull (adult bull, AB) testes from the same herd were randomly chosen, and transcriptome analysis was performed to find associations between spermatogenesis and transcriptome profiles. About 16,956 differentially expressed lncRNAs (DELs) and 7970 differentially expressed mRNAs (DEMs) were identified, and 3642 significantly relationship pairs were screened out based on co-expression analysis. Further Hub analysis obtained 6 lncRNAs, and 71 mRNAs regulated by them were enriched subsequently. Functional analysis results of differentially expressed Hub LncRNA-mRNA pairs showed that more positive cell cycle checkpoints, spindle assembly and the sister chromatids separation in AB testes indicated higher production rates of sperm in AB as compared to YB. This study reveals physiological changes and further broaden our understanding of lncRNA regulation of spermatogenesis in the fully mature testis according to the transcriptome profiles.

Analysis of combining ability and heterotic estimation on a complete Diallel cross involving five strains of common carp ( Cyprinus carpio ) from different geographical regions in West Java, Indonesia

Decline in growth performance of Indonesian common carp was suspected due to the high rate of inbreeding. This condition requires a breeding programme to improve the genetic quality of the fish. This study was conducted to evaluate the combining ability and to estimate the value of heterotic in the hybridization of common carp. Five strains of common carp were crossed using a complete diallel cross design to obtain five pure lines, 10 F1 crossings and 10 reciprocals. Each cross was cultured separately for 3 months in net-cage pond using a complete randomized design with three replications. All fish were fed twice a day at 3% of biomass with commercial pellet feed containing 28% crude protein. In the terminated period, 30 samples of each cross were measured and weighted to analyse the general and specific combining ability, and the heterosis. The results showed that Rajadanu and Sutisna strains have positive general combining ability (GCA), indicating these two strains have a high potential to be used as parent in the development of a hybrid population. Furthermore, the result of hybridization using one of these two strains generally produced a high positive specific combining ability (SCA) and heterosis. This study revealed that the non-additive, especially the dominant genetic variance more affected the expression of phenotypic characters of common carp. It implicates that the breeding programme to improve the genetics of common carp could be conducted through hybridization.

Mating pattern and pollen dispersal in an advanced generation seed orchard of Cunninghamia lanceolata (Lamb.) Hook.

Seed orchards represent the link between forest breeding and conifer production forests, and their mating patterns determine the genetic quality of seed orchard crops to a large extent. We genotyped the parental clones and their open pollination offspring in the third-generation seed orchard of Chinese fir using microsatellite markers and observed the synchronization of florescence in the seed orchard to understand the genetic diversity and mating structure of the seed orchard population. Genetic coancestry among parental clones was detected in the third generation seed orchard of Chinese fir, and the genetic diversity of the open-pollinated offspring was slightly higher than that of the parental clones. The external pollen contamination rate ranged from 10.1% to 33.7%, 80% of the offspring were produced by 44% of the parental clones in the orchard, and no evidence of selfing was found. We found that 68.1% of the effective pollination occurred within 50 m, and 19.9% of the effective pollination occurred in the nearest neighbors. We also found that successful mating requires about 30% of florescence overlap between males and females, and there was a significant positive correlation between male reproductive energy and male parental contribution. Our results provide a valuable reference for the management and design of advanced generation seed orchards.

Reproductive biology of Gazella arabica: Predictors of offspring weight and short- and long-term offspring survival.

Reproductive traits are central to organismal fitness, and so the factors influencing patterns of reproduction and offspring survival are at the heart of biology. Making use of breeding data collected over 16 years at the King Khalid Wildlife Research Centre in Saudi Arabia, we investigated the reproductive biology of Arabian gazelles Gazella arabica. Offspring survival was mainly a function of birth weight, with heavier offspring having higher survival rates than lighter offspring. However, while sons were heavier than daughters, daughters had higher survival rates. We could not find evidence that giving birth to sons negatively impacts offspring weight in the following year. We uncovered large narrow-sense heritability (h2) in offspring weight at birth, while maternal effects (m2) on birth weight were of lesser importance. However, maternal effects on offspring survival were strong until weaning age, while paternal effects dominated survival to sexual maturity and first reproduction. We propose that variation in maternal postnatal care might overshadow the effects of maternal inheritance of birth weights, while the overall strong heritability of weight at birth and the paternal effects on survival illustrates strong variance in sire fitness based on genetic quality, suggesting a role for sexual selection by female mate choice in wild populations.

CHARACTERIZATION PHENOTYPE AND GENETIC DISTANCE SOME OF THE NATIVE CHICKEN STRAINS IN JAMBI PROVINCE INDONESIA

Characterization is used to determine genetic quality which is used as a basis for consideration in selection and crossing. The research materials were Super Chicken, Bangkok, KUB, Sentul, Kampung and Merawang. Data including qualitative and quantitative. Descriptive and quantitative qualitative data used t-test, T2-Hotelling test and principal component analysis. Data processing using Minitab and MEGA-X program. The results showed that body weight, weight gain and body size of Super chickens were significantly different (P<0.05) higher than the other chickens. The closest genetic distance between the lines is Kampung dan Sentul chicken, then between Bangkok and Super chicken. Sentul and Kampung chicken have a close relationship with KUB, compared to Bangkok, Super and Merawang. Conclusion: Qualitative criteria for Super, Bangkok, KUB, Sentul, and Kampung are still very diverse, while Merawang chickens are more uniform. The highest body weight up to the age of 4 months is Super chicken, followed by Bangkok, KUB, Sentul, Kampung and Merawang. The increase in the body weight of chickens is achieved at the age of 2-3 months.

Inbreeding effects on telomeres in hatchling sand lizards (Lacerta agilis): An optimal family affair?

Telomeres are nucleotide-protein caps, predominantly at the ends of Metazoan linear chromosomes, showing complex dynamics with regard to their lengthening and shortening through life. Their complexity has entertained the idea that net telomere length and attrition could be valuable biomarkers of phenotypic and genetic quality of their bearer. Intuitively, those individuals could be more heterozygous and, hence, less inbred. However, some inbred taxa have longer, not shorter, telomeres. To understand the role of inbreeding in this complex scenario we need large samples across a range of genotypes with known maternity and paternity in telomere-screened organisms under natural conditions. We assessed the effects of parental and hatchling inbreeding on telomere length in >1300 offspring from >500 sires and dams in a population of sand lizards (Lacerta agilis). Maternal and paternal ID and their interactions predict hatchling telomere length at substantial effect sizes (R2 > .50). Deviation from mean maternal heterozygosity statistically predicts shorter offspring telomeres but this only when sibship is controlled for by paternal ID, and then is still limited (R2 = .06). Raw maternal heterozygosity scores, ignoring absolute deviation from the mean, explained 0.07% of the variance in hatchling telomere length. In conclusion, inbreeding is not a driver of telomere dynamics in the sand lizard (Lacerta agilis) study system.

Taiwan Biobank: A rich biomedical research database of the Taiwanese population

The Taiwan Biobank (TWB) is an ongoing prospective study of >150,000 individuals aged 20-70 in Taiwan. A comprehensive list of phenotypes was collected for each consented participant at recruitment and follow-up visits through structured interviews and physical measurements. Biomarkers and genetic data were generated from blood and urine samples. We present here an overview of TWB's genetic data quality, population structure, and familial relationship, which consists of predominantly Han Chinese ancestry, and highlight its important attributes and genetic findings thus far. A linkage to Taiwan's National Health Insurance database of >25 years and other registries is underway to enrich the phenotypic spectrum and enable deep and longitudinal genetic investigations. TWB provides one of the largest biobank resources for biomedical and public health research in East Asia that will contribute to our understanding of the genetic basis of health and disease in global populations through collaborative studies with other biobanks.

The relationship between depression, anxiety, quality of life levels, and the chronic kidney disease stage in the autosomal dominant polycystic kidney disease.

Decreased quality of life, anxiety, depression, and other negative psychosocial factors in autosomal dominant polycystic kidney disease (ADPKD) may lead to the patient's attitudes that reduce treatment effectiveness. We aimed to evaluate the relationship between the depression, anxiety, perceived social support, genetic psychosocial risk and quality of life levels, and chronic kidney disease (CKD) stage in ADPKD and to investigate the relationship between these variables/parameters and the dietary compliance that is an essential factor in the course of the disease. 100 ADPKD patients were enrolled in this cross-sectional study. EuroQol-5D-3L (EQ-5D-3L) health-related quality of life ındex, EuroQol-5D-3L visual analog scale (EQ-5D-3L VAS), multidimensional scale of perceived social support (MSPSS), patient health questionnaire (PHQ)-9, and genetic psychosocial risk ınstrument (GPRI) were applied to the patients. There is a relationship with negative regression coefficient between the CKD stage and the total scores of the EQ-5D-3L and EQ-5D-3L VAS scales (p < 0.000 and β = - 5.355, p < 0.000, and β = - 8.394, respectively). There is a relationship with positive regression coefficient between the CKD stage and MSPSS total score and level (p < 0.000 and β = 0.364, p < 0.000 and β = 0.331, respectively). There is no relationship between the CKD stage and GPRI total score (p = 0.800). In addition, there is a relationship with positive regression coefficient between the dietary compliance and EQ-5D and EQ-5D VAS total scores (p = 0.006 and β = 2.687, p = 0.004 and β = 3.148, respectively). There is a relationship with negative regression coefficient between the dietary adherence and PHQ-9 total score and CKD stage (p = 0.003, p = 0.006, and β = - 0.692, respectively). As the CKD stage increases in the ADPKD patients, the quality of life decreases, whereas the level of anxiety and depression increases. It has been seen that the ADPKD patients with more depressive complaints have less dietary compliance. In this particular patient group, the early detection and treatment of psychosocial difficulties and the work to improve the quality of life that affect the course of the ADPKD may be as important as the medical treatment. To determine the needs of ADPKD patients with multiple physical and psychosocial difficulties and to perform appropriate interventions, we think that there is a necessity for a specific scale that evaluates these effective components together in the ADPKD process.

Attitudes toward preimplantation genetic testing and quality of life among individuals with hereditary diffuse gastric cancer syndrome

BackgroundHereditary Diffuse Gastric Cancer (HDGC) syndrome is an autosomal dominant hereditary cancer predisposition associated with germline pathogenic/likely pathogenic variants in the CDH1 gene. Identifying early stage HDGC is difficult, and prophylactic measures can be effective in preventing incidence. Preimplantation Genetic Testing (PGT) can provide information about CDH1 variant status, HDGC risk, and limit familial transmission of CDH1 variants. To date, however, little is known about the attitudes of individuals with CDH1 variants towards PGT.MethodsGiven that little is known about the reproductive attitudes of individuals with HDGC, we recruited participants with CDH1 variants from a familial gastric cancer registry and administered a cross-sectional survey with open- and closed-ended response items. We assessed attitudes regarding PGT and the effect of HDGC on quality of life.ResultsParticipants (n = 21) were predominantly partnered (61.9%), had a personal cancer history (71.4%), and had biological children (71.4%). Interest in learning about PGT was high; 66.7% of participants were interested in PGT and 90.5% approved of healthcare providers discussing PGT with individuals with CDH1 variants. Attitudes regarding personal use were varied. Among all participants, 35% would not, 25% were uncertain, and 40% would use PGT. Personal philosophy and preferences for family and reproduction were key factors related to PGT attitudes. HDGC had moderate effects on participants’ quality of life, including social relationships, health behaviors, and emotional experiences including worry about cancer risk and guilt regarding familial implications.ConclusionPGT was identified by participants as acceptable for use in a variety of contexts and benefits of reproductive counseling involving PGT may extend beyond CDH1 carriers to family members’ reproductive behaviors. Dispositions towards PGT are governed by personal philosophy or belief systems. These findings can help guide providers counseling individuals with CDH1 variants.

Hormonal influences on women's extra-pair sexual interests: The moderating impact of partner attractiveness

Based on the idea that women are especially attracted to ancestral markers of male genetic quality when conceptive in their cycle, scholars have conjectured that increases in women's extra-pair sexual interests during the conceptive phase of the cycle are moderated by their primary partners' sexual attractiveness. Multiple studies have examined this prediction, with largely supportive but mixed results. The current study is the first to examine whether hormonal influences—thought to mediate cycle shifts—on women's extra-pair sexual interests are moderated by partner attractiveness. 213 naturally cycling, romantically involved women (181 attending multiple sessions) participated in up to four sessions over about a month. Estrogen and progesterone levels were measured multiple times across women's cycles. Male partner attractiveness moderated associations between progesterone levels (though not estrogen levels) and women's extra-pair sexual interests. A negative association between progesterone levels and extra-pair sexual interests, a composite measure, was observed for women with relatively unattractive partners. For women with relatively attractive partners, no clear association emerged. The interaction between progesterone and partner attractiveness was robust for women's interest in extra-pair sex, a component preregistered as exploratory. The interaction effect was also significant for the absolute intensity of women's extra-pair attraction (a component item referenced in the preregistration) but was non-significant for the frequency of women's extra-pair attraction relative to typical days (a composite component specified in the preregistration). These findings inform theoretical understandings of women's sexuality.