You have accessJournal of UrologyKidney Cancer: Basic Research II1 Apr 2010205 GENE EXPRESSION SIGNATURE ASSOCIATED WITH METASTASIS IN CLEAR CELL RENAL CELL CARCINOMA Jimsgene Sanjmyatav, Thomas Steiner, Mieczyslaw Gajda, Heiko Wunderlich, and Kerstin Junker Jimsgene SanjmyatavJimsgene Sanjmyatav More articles by this author , Thomas SteinerThomas Steiner More articles by this author , Mieczyslaw GajdaMieczyslaw Gajda More articles by this author , Heiko WunderlichHeiko Wunderlich More articles by this author , and Kerstin JunkerKerstin Junker More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.262AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Clear cell renal cell carcinoma (ccRCC) has the highest rate of metastases and the poorest survival among the common renal malignancies. Until now, it is impossible to predict the prognosis of an individual prognosis and therapy selection. Therefore the aim of this study was to identify potential biomarkers for metastasing ccRCC which have prognostic relevance for an optimal clinical management. METHODS Gene expression patterns of 16 metastasized and 18 non-metastasized ccRCC were examined using topic defined microarrays (Piqorarray, Miltenyi Biotec). The microarrays included 1056 genes which belong to the pathways for cytokine signalling and regulation, apoptosis, signal transduction, stress and inflammation and genes for cell cycle, DNA repair, chemokines, chemokine receptors, complement system, cytokines, cytokine receptors and extracellular matrix proteins. The analysis of the microarraydata was performed using Qlucore Omics Gene Expression Explorer 2.0. For the validation of differentially expressed genes QRT-PCR using Universal Probe Library Probes (Roche) was applied. RESULTS In a significance analysis for microarrays (FDR 0%) a gene signature discriminating between the metastasized and non-metastasized tumors were identified. Genes MMP16, BCL2L2, CEACAM5, FRA2, SPARC, CD151, IKBA showed a good discrimating behavior. Three genes (CD151, IKBA und SPARC: t/Fstatistic: p<0,0001) were significantly decreased (more than 4fold) in the group of metastasized tumours. On contrary the genes MMP16, BCL2L2, CEACAM5, FRA2 were significantly (t/Fstatistic: p<0,0001) enriched and showed more than 4fold increased expression in metastasized tumors. CONCLUSIONS Genes for the discrimination between metastasized and non-metastasized tumors (FDR 0%) are consistently detected on microarray analysis proving their potential as putative prognostic marker. Based on these data it seems possible to define the metastatic potential of primary tumours and to optimise the clinical management. Jena, Germany© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e81 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jimsgene Sanjmyatav More articles by this author Thomas Steiner More articles by this author Mieczyslaw Gajda More articles by this author Heiko Wunderlich More articles by this author Kerstin Junker More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...
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