We developed 21 near-isogenic lines (NILs) in the genetic background of the Indica Group rice ( Oryza sativa L.) cultivar IR64, with each containing one of the 11 blast resistance genes: Pish , Piz , Piz-5 , Pi9 (t), Pii , Pi3 , Pik , Pik-h , Pik-p , Pi1 , and Pi7 (t). The NILs were confirmed to harbor these genes by their reaction patterns to standard differential blast isolates and by analyses for graphical genotypes using DNA markers located on the same chromosome regions as the target resistance genes. The agronomic traits of these NILs, such as days to heading and yield components, were also investigated and found to be very similar to those of IR64, even when grown under the dramatically different environmental conditions of the temperate and subtropical regions of Japan. These NILs and their genetic information will be used as gene sources for Indica Group rice breeding programs, to improve resistance to blast disease, and as a multiline variety that will also be useful as an alternative approach to the development of the durability of rice resistance to blast disease in tropical areas, especially in irrigated lowland ecosystems.