This paper provides an in-depth analysis and research on piano timbre teaching in the context of artificial intelligence interaction, a bold vision of piano teaching, proposes a feasible solution in terms of teaching modules in intelligent piano teaching for senior teachers, and proposes an implementation path for the integration of intelligent piano and piano teaching from the four main blocks of piano teaching. Based on the multiplicative harmonic model of monophonic signal, combined with the variability of timbre characteristics, an audio synthesis model with editable timbre is proposed, and the experimental results show that editing the timbre parameters in the model can realize timbre modification, and the synthesized timbre conforms to the piano timbre characteristics. Based on the timbre analysis and the timbre synthesis model, a piano timbre library generation system is designed. The detailed design of the software modules such as audio file reading and writing, audio information analysis, timbre parameter acquisition, timbre synthesis, and simulated performance is given. The system can generate piano timbre libraries of different qualities flexibly and meet the requirements of timbre realism. The teaching experiment designed for teaching practice from solo teaching, and the practice target is first-year undergraduate students in the university, and the practice period is six weeks, and finally, the feasibility of intelligent piano teaching application is analysed by combining the experimental results. Through the teaching objectives, teaching content, and teaching methods, teaching environment reflects intelligent piano teaching to make up for the limitations of traditional piano teaching. Analyse the development trend of intelligent piano teaching in the context of artificial intelligence interaction, and explore the value of intelligent piano teaching.