Diamond-Blackfan anemia syndrome (DBAS) is an inherited bone marrow failure disorder that typically presents in infancy as hypoplastic anemia and developmental abnormalities in approximately 50 % of cases. DBAS is caused by haploinsufficiency in one of 24 ribosomal protein genes, with RPS19 mutations accounting for 25 % of cases. We generated iPSC lines from two patients with different heterozygous RPS19 mutations (c.191 T > C and c.184C > T) and isogenic lines in which the mutations were corrected by Cas9-mediated homology directed repair.