Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors

Katerina Vlahos,Koula Sourris,Jia Yi Kuah,Alison Graham,Annabelle Suter,Sara E Howden,Edouard G Stanley,Andrew G Elefanty

doi:10.1016/j.scr.2023.103109

Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors

Katerina Vlahos, Koula Sourris + Show 6 more

Open Access

https://doi.org/10.1016/j.scr.2023.103109

Copy DOI

Journal: Stem Cell Research	Publication Date: May 3, 2023
License type: cc-by

Affiliation: Murdoch Children's Research Institute, University of Melbourne

#iPSC Lines #Peripheral Blood Mononuclear Cells + Show 8 more

Abstract
Full-Text PDF
Similar Papers

Abstract

We describe the generation and characterisation of five human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, and pluripotency was validated by flow cytometry and immunofluorescence of pluripotency markers, and their differentiation into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms.

Full Text