Generation Of Hydroxyl Radicals Research Articles

Copper-based nanodots as efficient biomimetic antibiotics for the treatment of oral bacterial infections

Oral infectious diseases caused by a variety of pathogenic bacteria seriously affect the quality of life. However, these diseases remain a clinical challenge because of the lack of simple, safe, and universal prophylactics. To address these limitations, we synthesize CuOx nanodots (CuOx NDs) with excellent Fenton-like reaction activity and utilize them in the treatment of oral bacterial infections. Different from other complicated approaches, CuOx NDs are rationally prepared using a facile one-pot aqueous synthesis. In the presence of H2O2, these well-developed CuOx NDs can efficiently catalyze the generation of hydroxyl radicals (·OH) around oral pathogens, leading to the death of various bacteria. Meanwhile, results of biosafety indicate the high biocompatibility and extremely low toxicity of these CuOx NDs. After understanding the admirable in vitro antibacterial effect of CuOx NDs in the presence of H2O2, we further explore their in vivo antibacterial performance on several classical animal models including oral mucosal wound model, intragingival bacteria-infected model, and the periodontal infection model. As expected, these CuOx NDs with wide-spectrum antibacterial activity can serve as high-performance antibacterial reagents for the treatment of various oral bacterial infections with the help of H2O2. In brief, current nanoplatform can act as efficient antibiotics against oral pathogens with broadening the biomedical applications of copper-based nanomaterials.

Samarium doped carbon dots for near-infrared photo-therapy

Photo-assisted tumor therapy, encompassing photodynamic therapy (PDT) and photothermal therapy (PTT), has garnered considerable attention as a promising approach for tumor treatment owing to its stimuli-responsive nature and noninvasive characteristics. Carbon dots (CDs) have recently emerged as a novel type of nanomaterial with great potential in the field of anticancer therapy. However, the development of multifunctional CDs nano-platforms that integrate multiple therapeutic performance remains a challenge, especially in near-infrared (NIR) window. In this study, we synthesized samarium-doped carbon dots (Sm/CDs) via a one-step hydrothermal process for fluorescence imaging guided PTT/PDT in NIR-II window. Sm/CDs demonstrated remarkable absorption and emission properties in the NIR region, enabling efficient generation of heat energy, superoxide (•O2–) and hydroxyl radicals (•OH) under NIR irradiation. The photothermal conversion efficiencies of Sm/CDs reached up to 34.12 % and 24.38 % when exposed to 808 nm and 1060 nm wavelengths, respectively. Moreover, long-term toxicity experiments conducted in vivo as well as fluorescence experiments confirmed the potential of such functional CDs for fluorescence imaging and excellent biocompatibility. It is noteworthy that the tumor is nearly eradicated when exposed to either 808 nm or 1060 nm, demonstrating significant efficacy in both scenarios. This study provides a multifunctional CDs nano-platform that offers a promising bimodal phototherapeutic strategy to enhance the synergistic treatment of tumors through PDT and PTT.

Enhanced peroxidase-like activity of Cu–Cu2O composite film through PtPd immobilization for colorimetric glucose detection

In this study, Cu–Cu2O/PtPd nanocomposites were synthesized and characterized for their peroxidase-like enzyme activity. X-ray diffraction and energy dispersive X-ray spectroscopy analyses confirmed the successful synthesis of the nanocomposites, which exhibited a flower-like morphology and a more uniform dispersion than Cu–Cu2O. The catalytic activity of Cu–Cu2O/PtPd was evaluated using the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB), finding that Cu–Cu2O/PtPd outperformed Cu–Cu2O. The optimal temperature and pH for the catalytic activity of Cu–Cu2O/PtPd were determined to be 40 °C and pH 4.0, respectively. A kinetic analysis revealed that Cu–Cu2O/PtPd followed Michaelis–Menten kinetics and exhibited a higher affinity toward TMB than the horseradish peroxidase enzyme. The catalytic mechanism of Cu–Cu2O/PtPd involved the generation of hydroxyl radicals, which facilitated the oxidation of TMB. Furthermore, the Cu–Cu2O/PtPd nanocomposite was successfully applied for the colorimetric detection of glucose, demonstrating a linear range of 8–90 μM, a detection limit of 2.389 μM, and high selectivity for glucose over other sugars.

Surface Modification of p‐type ZnO Nanorods by Nitrogen Doped SiO2 Dots as an Efficient Solar Photocatalyst for Degradation of Ciprofloxacin in Water

AbstractA simple two step hydrothermal method is developed for synthesizing p‐type zinc oxide (ZnO) nanorods surface modified with nitrogen doped silicon dioxide dots (ZnO/N‐SiO2). The structure, morphology, and chemical compositions are confirmed by X‐ray diffraction, Raman spectroscopy, Fourier transformed infrared spectroscopy, X‐ray photoelectron spectroscopy, transmission electron microscopy, and by Mott‐Schottky studies. The N‐SiO2 dots on the surface of p‐type ZnO nanorods are formed during hydrothermal treatment at 90 °C (i.e., at 363 K), named as [ZnO/N‐SiO2]363K. While N‐SiO2 dots are embedded in the ZnO nanorods of the batch synthesized at 453 K. The band gap of the batches of ZnO/N‐SiO2 are wider (3.24–3.30 eV) than the pristine ZnO (3.16 eV). The modification of ZnO nanorods by the N‐SiO2 dots is corroborated by changes in the flat band potential, revealed from Mott‐Schottky measurements. The EPR and photoluminescence studies confirm p‐type ZnO attributable to zinc vacancies (VZn). The batch [ZnO/N‐SiO2]363 exhibits maximum photocatalytic degradation of ciprofloxacin in water. The specific rate constant is k′ = 0.97 min−1 g−1, which is nearly three times higher than that exhibited by the irregularly spherical pristine ZnO nanoparticles (k′ = 0.36 min−1 g−1). The enhanced photocatalytic degradation is attributed to holes mediated hydroxyl radical generation. The degradation mechanism is proposed by carrier mobility studies, radical scavenging studies and by identifying the degradation products.

Novel SnO2-modified hydrozincite photocatalyst: Material design and application in efficient micropollutants degradation in wastewater

In this work novel photocatalysts have been synthesized based on hydrozincite and SnO2 with varying mol% of SnO2 in hydrozincite. The photocatalysts were prepared via chemical co-precipitation using thermal urea hydrolysis, without calcination or activation processes, and were applied in the photodegradation of micropollutants (in this case, several phenolic compounds). Characterization of the materials using various techniques revealed that the generation of hydroxyl radicals, holes, and superoxide radicals played crucial roles in the photodegradation process. The best photocatalyst was achieved with a 10 mol% SnO2 content in hydrozincite, which shows the highest rate in the pollutants degradation (higher than 93% in all the cases), also higher than TiO2-P25This study presents promising insights for developing highly efficient photocatalytic materials for environmental remediation applications.

Electronic structure modulation of iron sites with fluorine coordination enables ultra-effective H2O2 activation

Electronic structure modulation of active sites is critical important in Fenton catalysis as it offers a promising strategy for boosting H2O2 activation. However, efficient generation of hydroxyl radicals (•OH) is often limited to the unoptimized coordination environment of active sites. Herein, we report the rational design and synthesis of iron oxyfluoride (FeOF), whose iron sites strongly coordinate with the most electronegative fluorine atoms in a characteristic moiety of F-(Fe(III)O3)-F, for effective H2O2 activation with potent •OH generation. Results demonstrate that the fluorine coordination plays a pivotal role in lowering the local electron density and optimizing the electronic structures of iron sites, thus facilitating the rate-limiting H2O2 adsorption and subsequent peroxyl bond cleavage reactions. Consequently, FeOF exhibits a significant and pH-adaptive •OH yield (~450 µM) with high selectivity, which is 1 ~ 3 orders of magnitude higher than the state-of-the-art iron-based catalysts, leading to excellent degradation activities against various organic pollutants at neutral condition. This work provides fundamental insights into the function of fluorine coordination in boosting Fenton catalysis at atomic level, which may inspire the design of efficient active sites for sustainable environmental remediation.

MOF derived highly dispersed bimetallic Fe-Ni nitrogen-doped carbon for enhanced peroxymonosulfate activation and tetracycline degradation

Transition metals as activators of peroxymonosulfate (PMS) are effective catalysts for the degradation of organic pollutants. The construction of multiple active sites is the key for enhanced PMS activation. Herein, we prepared ZIF-8 derived Fe- Ni nitrogen-carbon with highly dispersed bimetallic active sites, achieving a small amount of highly efficient catalytic performance for tetracycline (TC) degradation. The Fe and Ni elements in the catalyst were uniformly dispersed, resulting in a high specific surface area of 367 m2/g. Even at low metal concentrations (Fe=0.83%, Ni=0.33%), Fe-Ni-N-C/PMS showed superior performance in degrading total carbon TC. Its reaction rate constant was 1.71 times higher than Fe-N-C/PMS and 2.13 times higher than Ni-N-C/PMS. This significant improvement was attributed to the synergistic effect of the bimetallic components and the highly dispersed active sites. The mechanism was proposed that Fe-Ni-N-C activates PMS to generate hydroxyl radicals (•OH), sulfate radicals (SO4•−), superoxide radicals (O2•−), and singlet oxygen (1O2) to further degrade TC. Among them, 1O2 and SO4•−were the main reactive oxygen species. This work has provided a new insight into the development of transition metals with multiple active sites for the degradation of organic pollutants.

Photocatalytic-based Titanium Dioxide and Its Application in Reducing Organic Pollutant of River Water

The Indonesian region of Sumatra has many rivers, and several of them are currently polluted by household and industrial wastes. Due to this, the river water looks cloudy and not clear. An easy method is needed to reduce the impact of this organic waste pollution. Photocatalysts are known for their ability to destroy organic pollutants which are environmentally friendly, cheap, and have the potential to be widely developed on a large scale. The principal mechanism of photocatalyst are generating the hydroxyl radicals (OH), which act as strong oxidants to mineralize organic contaminants. These principal photocatalyst mechanism includes the generation of hydroxyl radicals (OH), which act as strong oxidants to mineralize organic contaminants. A handy sprayimmobilization method of TiO2 powders on the solid plastic buffer has been utilized in a destroying effluent system. The photocatalytic activity of the TiO2-immobilized transparent plastic were evaluated by the degradation of organic contaminant under sunlight irradiation. The titania catalyst have the most potential destroying impact of about 98% after 10 days of sun irradiation. This investigation proved the potential of titanium oxide in handling organic waste in rivers.
 Keywords: titanium dioxide, photocatalytic, water treatment, river

Band Structure Tuning via Pt Single Atom Induced Rapid Hydroxyl Radical Generation toward Efficient Photocatalytic Reforming of Lignocellulose into H2.

Photocatalytic lignocellulose reforming for H2 production presents a compelling solution to solve environmental and energy issues. However, achieving scalable conversion under benign conditions faces consistent challenges including insufficient active sites for H2 evolution reaction (HER) and inefficient lignocellulose oxidation directly by photogenerated holes. Herein, it is found that Pt single atom-loaded CdS nanosheet (PtSA-CdS) would be an active photocatalyst for lignocellulose-to-H2 conversion. Theoretical and experimental analyses confirm that the valence band of CdS shifts downward after depositing isolated Pt atoms, and the slope of valence band potential on pH for PtSA-CdS is more positive than Nernstian equation. These characteristics allow PtSA-CdS to generate large amounts of •OH radicals even at pH 14, while the capacity is lacking with CdS alone. The employment of •OH/OH- redox shuttle succeeds in relaying photoexcited holes from the surface of photocatalyst, and the •OH radicals can diffuse away to decompose lignocellulose efficiently. Simultaneously, surface Pt atoms, featured with a thermoneutral , would collect electrons to expedite HER. Consequently, PtSA-CdS performs a H2 evolution rate of 10.14 µmol h-1 in 1m KOH aqueous solution, showcasing a remarkable 37.1-fold enhancement compared to CdS. This work provides a feasible approach to transform waste biomass into valuable sources.

Efficient hydrogen recovery from wastewater treatment by piezo-photocatalytic heterostructure

Hydrogen (H2) recovery during wastewater treatment has been considered as a promising direction to accomplish environmental sustainability. However, the effective H2 evolution in contaminants degradation process was still a major challenge. In this research, an advanced piezo-photocatalytic induced hydro-energy system is proposed, in consideration of improving the separation and transfer efficiency of photogenerated electrons and holes. As expected, the catalytic performance was significantly enhanced under the stress of ultrasound-induced piezoelectric field (major nitenpyram degradation within 60 min, H2 evolution was 746.56 μmol g−1 h−1). Experiments and density functional theory calculations illustrated that the introduced piezoelectric field directionally tuned the energy band structure of heterostructure, triggering a change in the electron transfer path within the heterostructure, which facilitated the major electrons transferred to zinc oxide (H2 evolution) and holes accumulated in molybdenum sulfide (hydroxyl radical generation), further accelerating contaminants degradation and H2 evolution. Furthermore, the in-depth mechanism of contaminants degradation and H2 evolution under the influence of piezoelectric field was further explored. The piezo-photocatalytic system of this work first accomplished H2 recovery in wastewater treatment on heterostructure, providing a constructive strategy for the design of environmentally sustainable catalytic systems.

Highly fast and efficient coupled Fe/Sn galvanic nanocatalyst with H2O2 Fenton-like process for synergistic reduction–oxidation of nitroaromatic compound

Herein, the reinforced electron transfer ability of nano-galvanic Fe/Sn bimetallic particles deposited on talc for catalytic elimination of 2,4-dinitrotoluene (2,4-DNT) was investigated at different conditions. FESEM, XRD and FTIR characterization confirmed the successful and uniform formation of bimetallic galvanic particles on support. Ultrafast and intensified galvanic cell effects of bimetallic core-shell Fe/Sn nanoparticles enabled the nanocomposite to reach solely to 2,4-DNT removal efficiency of 91 % under the optimized condition. In addition, the synergistic combination of Fe/Sn galvanic nanocatalyst and H2O2 heterogeneous Fenton-like system, which took advantage of bimetallic Fe/Sn particles potential as viable adsorbent-reductant in pollution removal and effective source for generation of hydroxyl radicals, achieved 100 % 2,4-DNT removal within 10 min. The performance of this remediating process was evaluated under different nanocatalyst amount, pH, temperature, contaminant concentration, and H2O2 concentration. The removal process was in agreement with the pseudo-second-order kinetic model and the modified Langmuir isotherm model. Findings elucidated that the removal mechanism involved the electrostatic adsorption, galvanic oxidation–reduction and co-precipitation by nanocomposite, which was intensified by synergistic reaction of the hydroxyl radicals with pollutant. Overall, this study investigated the high potential of the combinational nZVI bimetallic galvanic nanoparticles and Fenton remediating processes, and the findings demonstrated the superior degradation capacity of the process as an innovative remediating approach.

Characterization and impact of dissolved gases in a high-frequency flow reactor

Addressing the growing demands for sustainable green technology in chemical processes, we aim to develop a novel high-frequency ultrasonic flow reactor that utilizes converting ultrasound waves, resulting in intense localized acoustic pressure fields. Our hypothesis that our design of converging waves will establish a uniform spatial distribution of cavitation activity with high energy density, thereby augmenting the rate of radical formation and significantly enhancing overall reactor performance. To validate the hypothesis, KI and terephthalic acid dosimetry were performed. A comprehensive calorimetric study was employed for acoustic power, yielding valuable insight into the energy dynamics of the system. Furthermore, our research systematically assesses the impact of dissolved gases and varying flow rates on reactor performance. Notably, we observe that dissolved gases in the reaction medium exert a substantial influence on reactor efficiency. Additionally, an increased flow rate beyond the optimum decelerates hydroxyl radical generation due to a lower residence time. The detailed characterization, facilitated by dosimetry techniques, unveils the potential of the sonochemical reactor for industrial applications.

Band gap regulation of LaFeO3 via doping Sr for efficient conversion of coke and steam

Coking is bottleneck problem during steam cracking processes, so strategies for online catalytic conversion of coke are vitally important for ethylene production. In this work, a method for regulating the band bap of perovskites to enhance hydroxyl radical generation is proposed to promote the catalytic conversion of coke. A series of ternary La1-xSrxFeO3 (x = 0, 0.2, 0.4, 0.6, 0.8, and 1) perovskites were successfully prepared by sol-gel method. Experimental and DFT calculations show that Sr doped at A sites facilitates a reduction in the band gap and the promotion of electronic transitions in the perovskites. Moreover, water molecule adsorption, decomposition, and intermediate transition state desorption are improved. Using 10 wt% LSF-0.6 catalyst, a water flow rate of 0.167 mL/min, reaction temperature of 900 °C, and reaction time of 120 min, a coke conversion rate of 85.56% is achieved. This work provides a theoretical basis for in-situ high-efficiency coke removal.

Analysis of Bioactive Aroma Compounds in Essential Oils from Algerian Plants: Implications for Potential Antioxidant Applications.

In samples of Artemisia campestris (AC), Artemisia herba-alba (AHA) and Salvia jordanii (SJ) essential oils, up to 200 distinct volatile compounds were identified. Using headspace solid-phase microextraction combined with gas chromatography-olfactometry-mass spectrometry (HS-SPME-GC-O-MS), different panelists detected 52 of these compounds. This study offers the most detailed analysis of bioactive compound profiles conducted so far. The most abundant compounds identified were curcumene, making up 12.96% of AC, and camphor, constituting 21.67% of AHA and 19.15% of SJ. The compounds with the highest odor activity value (OAV) were (E,Z)-2,4-nonadienal (geranium, pungent), 3-nonenal (cucumber) and 2-undecenal (sweet) in AC, AHA and SJ, respectively. AHA essential oil showed significant antioxidant activity (IC50 = 41.73 ± 4.14 mg/g) and hydroxyl radical generation (hydroxylation percentage = 29.62 ± 3.14), as assessed by the diphenylpicrylhydrazyl (DPPH) method. In terms of oxygen radical absorbance capacity (ORAC), the strongest antioxidant activity was obtained for SJ essential oil (antioxidant activity of the essential oils, AOX = 337.49 ± 9.87).

Ultrafine FeCuAgCeGd–based high–entropy nanozyme: Preparation, catalytic mechanism, and point–of–care detection of dopamine in human serum

Point–of–care testing (POCT) plays vital role in clinic field, especially in resource–limited areas. The nanozyme–based colorimetric platform is the potential strategy for POCT. Therefore, a high–entropy allay nanozyme (HEAzyme) was prepared via formaldehyde–assisted metal–ligand crosslinking method using multi–metal (FeCuAgCeGd) species. The obtained FeCuAgCeGd–HEAzyme showed coordination nanoparticles with ultrafine size (average size was approximately 5 nm). The peroxidase–like (POD–like) activity of FeCuAgCeGd–HEAzyme was verified through density functional theory (DFT) calculations, demonstrating the capability of FeCuAgCeGd–HEAzyme to catalyze H2O2 and generate hydroxyl radicals (•OH). These radicals can oxidize colorless 3,3′,5,5′–tetramethylbenzidine (TMB) to blue oxTMB. Dopamine (DA) scavenges •OH, resulting in an “on–off” effect for TMB oxidation. Subsequently, an FeCuAgCeGd–HEAzyme colorimetric sensor was developed for DA detection, which features a low detection limit of 0.77 µM and a linear range of 5.0–70.0 µM. The sensor demonstrated a recovery ratio in spiked serum samples ranging from 97.7 % to 104.3 % with high stability and favorable selectivity. Furthermore, an FeCuAgCeGd–HEAzyme POCT platform was designed for on–site detection of DA, achieving a reliable linear detection range (5.0–70.0 µM) and a low detection limit (5.69 µM). This platform offers a facile and efficient strategy for rapid, visual, and on–site DA detection in clinical settings with advantages of cost–effectiveness and ease of operation.

Ultrasound-activated piezo-hot carriers trigger tandem catalysis coordinating cuproptosis-like bacterial death against implant infections

Implant-associated infections due to the formation of bacterial biofilms pose a serious threat in medical healthcare, which needs effective therapeutic methods. Here, we propose a multifunctional nanoreactor by spatiotemporal ultrasound-driven tandem catalysis to amplify the efficacy of sonodynamic and chemodynamic therapy. By combining piezoelectric barium titanate with polydopamine and copper, the ultrasound-activated piezo-hot carriers transfer easily to copper by polydopamine. It boosts reactive oxygen species production by piezoelectrics, and facilitates the interconversion between Cu2+ and Cu+ to promote hydroxyl radical generation via Cu+ -catalyzed chemodynamic reactions. Finally, the elevated reactive oxygen species cause bacterial membrane structure loosening and DNA damage. Transcriptomics and metabolomics analysis reveal that intracellular copper overload restricts the tricarboxylic acid cycle, promoting bacterial cuproptosis-like death. Therefore, the polyetherketoneketone scaffold engineered with the designed nanoreactor shows excellent antibacterial performance with ultrasound stimulation and promotes angiogenesis and osteogenesis on-demand in vivo.

Preparation of graphene supported copper composite materials and study on the catalytic activity of phenol hydroxylation

The hydroxylation of phenol to synthesize dihydroxybenzene (DHB) has recently attracted considerable attention due to its inherent advantages, including its simplicity in industrial applications, environmental friendliness, and remarkable selectivity towards DHB. The hydrothermal method was used to prepare graphene supported copper composites (Cu/GNS) with excellent catalytic properties, which were applied in the phenol hydroxylation for DHB preparation. Cu/GNS with controllable copper content were successfully obtained, achieving the homogeneous dispersion of copper ions on the graphene surface while preventing graphene agglomeration. During catalysis, graphene serves as an electron transport conduit, enhancing the exposure of reactive active sites and facilitating the generation of hydroxyl radicals. The π-π interactions between graphene and phenol enhance the propensity for collision between phenol and hydroxyl radicals. The total selectivity of DHB reached 98.4%, and the phenol conversion reached 85.1%. The composites exhibited high stability, with 80.5% phenol conversion and 93.7% DHB total selectivity even after five reuse cycles.

Synergistic experimental study by a solar pilot evaporation system for the volume reduction and antibiotics photocatalytic degradation of liquid digestate

The challenges posed by the large volume, low nutrient content, and high antibiotic concentrations in the liquid digestate from anaerobic digestion are becoming increasingly prominent. In this study, a solar pilot system was used for the evaporation and volume reduction of the liquid digestate and the photocatalytic degradation of antibiotics in the liquid digestate. First, given the difficulty in recovering the powdered catalyst, dolomite-supported g-C3N4 (DCN) was synthesized using a simple one-pot method, and its photocatalytic properties were investigated. The results indicated that the photocatalytic performance of DCN was excellent when the urea-to-dolomite doping ratio was 1:8, and the degradation efficiency of tetracyclines (TCs) in the real liquid digestate reached 90.65 % when the amount of DCN/1:8 was 0.625 g/mL. The TCs degradation efficiency increased as the initial pH of the liquid digestate decreased, reaching 95.33 % at an initial pH of 4, which could be attributed to the acidic conditions that are favorable for the generation of hydroxyl radicals and excited oxygen. Furthermore, the DCN was filled directly into a solar pilot system, which was employed for the evaporation reduction treatment of the liquid digestate as well as the photocatalytic degradation of antibiotics under optimal process conditions. The pilot experiment showed that the average TCs degradation efficiency rose from 57.54 % to 95.27 % with the addition of DCN/1:8 to the solar evaporation system. After a 4-day replicated experiment, the average TCs degradation efficiency reached 90.64 %. Importantly, DCN/1:8 had less of an effect on the evaporation efficiency of the liquid digestate and the recovery of key nutrients, such as NH4+-N. This study aims to demonstrate the engineering, theoretical, and technological feasibility of sunlight photocatalytic degradation of antibiotics in complex water quality systems and to provide a reference for the reduction and harmless treatment of liquid digestate.

Self-Promoted Hydroxyl Radical Releasing Magnetic Zn@Fe Particles.

Semiconductor photocatalysts, such as TiO2 and ZnO, have garnered significant attention for their ability to generate hydroxyl radicals, offering various practical applications. However, the reliance on UV light to facilitate electron-hole separation for hydroxyl radical production poses limitations. In this study, a novel approach is presented utilizing Zn@Fe core/shell particles capable of generating hydroxyl radicals without external energy input. The generation process involves electron donation from Zn to O2, resulting in the formation of radical species .O2 -/H2O2, followed by Fe-catalyzed conversion of H2O2 into hydroxyl radicals through the Fenton reaction. The release of .OH imparts good antimicrobial and antiviral properties to the Zn@Fe particles. Furthermore, the inclusion of Fe confers magnetic properties to the material. This dual functionality holds promise for diverse potential applications for the Zn@Fe particles.

HOFs structured CN template enables the dual regulation of Cu–O and Cu–N active sites in porous Cu-g-CN for synergistic photo-Fenton process

Photo-Fenton catalysis with strong oxidation potential and cyclic stability represents a promising technique for addressing the escalating environmental challenges. However, the photo-Fenton activity using Fe/Cu doped graphitic carbon nitride (g-CN) catalysts is significantly hindered by the limited reaction interface and non-tunable surface-active sites. Herein, we present a novel CN polymer type hydrogen bonded organic framework (CN-HOFs) template derived Cu-g-CN (Cu/CH-g-CN) based hybriding catalyst (CN-HOFs/Cu/CH-g-CN), which process greatly optimized surface features for high-performance photo-Fenton reaction with exceptional durability in a broad pH range of 3–11. Importantly, the distinctive porous structure of CN-HOFs template offers not only high specific surface area, but also the possibility to regulate Cu active sites. The coexistence of Cu–O and Cu–N active sites is identified by XPS, which facilitates the separation and migration of photogenerated charges, expediting the redox cycle. The synergistic photo-Fenton reaction boosted by the porous CN-HOFs structure and the dual Cu active sites (Cu–O and Cu–N) was verified by degradation activity of tetracycline. Under visible light irradiation at a concentration of 20 mg/L, the degradation efficiency reaches nearly 100% within 20 min, with exceptional cycling stability. Further investigations have revealed that the Cu–O active site is conducive to the adsorption of organic pollutants, while the Cu–N active site promotes electron transfer, accelerates the cyclic conversion of Cu+ and Cu2+. The dual copper active site is beneficial for the generation of hydroxyl radicals and the stable operation of the catalyst, resulting in significant photo-Fenton activity. This work underscores the importance of regulating the nanoscale morphology and electronic structure of photofunctional catalysts, not only to increase the active sites for photo-Fenton reactions but also to facilitate charge transfer and the cycling of active metal ions, thereby improving the sustainability of heterogeneous photo-Fenton process.