General Requirements Research Articles

Bootstrapping Lagrangian perturbation theory for the large scale structure

We develop a model-independent approach to Lagrangian perturbation theory for the large scale structure of the universe. We focus on the displacement field for dark matter particles, and derive its most general structure without assuming a specific form for the equations of motion, but implementing a set of general requirements based on symmetry principles and consistency with the perturbative approach. We present explicit results up to sixth order, and provide an algorithmic procedure for arbitrarily higher orders. The resulting displacement field is expressed as an expansion in operators built up from the linear density field, with time-dependent coefficients that can be obtained, in a specific model, by solving ordinary differential equations. The derived structure is general enough to cover a wide spectrum of models beyond ΛCDM, including modified gravity scenarios of the Horndeski type and models with multiple dark matter species. This work is a first step towards a complete model-independent Lagrangian forward model, to be employed in cosmological analyses with power spectrum and bispectrum, other summary statistics, and field-level inference.

A conceptual data modeling framework with four levels of abstraction for environmental information

Environmental data generated by observation infrastructures and models is widely heterogeneous in both structure and semantics. The design and implementation of an ad hoc data model for each new dataset is costly and creates barriers for data integration. On the other hand, designing a single data model that supports any kind of environmental data has shown to be a complex task, and the resulting tools do not provide the required efficiency. In this paper, a new data modeling framework is proposed that enables the reuse of generic structures among different application domains and specific applications. The framework considers four levels of abstraction for the data models. Levels 1 and 2 provide general data model structures for environmental data, based on those defined by the Observations and Measurements (O&M) standard of the Open Geospatial Consortium (OGC). Level 3 incorporates generic data models for different application areas, whereas specific application models are designed at Level 4, reusing structures of the previous levels. Various use cases were implemented to illustrate the capabilities of the framework. A performance evaluation using six datasets of three different use cases has shown that the query response times achieved over the structures of Level 4 are very good compared to both ad hoc models and to a direct implementation of O&M in a Sensor Observation Service (SOS) tool. A qualitative evaluation shows that the framework fulfills a collection of general requirements not supported by any other existing solution.

Comparison of Specific Exemption Regulations for Consumer Products Containing Radioactive Isotopes

Background: Practices involving radionuclides at levels below the International Atomic Energy Agency (IAEA) generic exemptions are exempt from regulation without further consideration. Practices involving radionuclides at levels above those generic exemptions may also be exempt from regulation if they meet certain conditions. These are known as specific exemptions, and each country has established its own specific exemption criteria based on the conditions set out in the IAEA General Safety Requirements (GSR) Part 3. Those conditions relate to the physical or chemical form of the radioactive material as well as to its use or the means of its disposal.Materials and Methods: The specific exemption criteria of eight countries (i.e., the United States of America [US], Japan, France, China, Australia, Canada, the United Kingdom of Great Britain and Northern Ireland [UK], and Germany) were analyzed. Their similarities and differences as compared with the specific exemption criteria of the Republic of Korea (ROK) were analyzed, and suggestions for revision of the Korean regulations were formulated.Results and Discussion: Each country’s specific exemption criteria are defined based mostly on the IAEA criteria but tailored to its domestic circumstances. The nine countries with their specific exemption criteria can be broadly categorized into three groups: nuclide-specific exemptions for specific products (the ROK and the US), common criteria for all nuclides without specification of particular products (Japan, France, and China), and both specific and common criteria (Australia, Canada, the UK, and Germany).Conclusion: The specific exemption criteria of the different countries examined in this study could be helpful in reviewing the ROK’s specific exemption criteria. Development of common criteria alongside specific criteria for products requiring special attention may be a good way to determine whether new consumer products containing radioisotopes should be regulated.

Engineering consensus in static networks with unknown disruptors

Distributed control can increase system scalability, flexibility, and redundancy. Foundational to such scalability via decentralisation is consensus formation, by which decision-making and coordination are achieved. However, decentralised multi-agent systems are inherently vulnerable to disruption. To develop a resilient consensus approach, inspiration is taken from the study of social dynamics; specifically, the Deffuant Model which evaluates the impact of tolerance in social systems. A dynamic protocol is presented enabling efficient consensus to be reached with an unknown number of disruptors present within a multi-agent system. By inverting typical social tolerance, agents filter out extremist non-standard opinions that would drive them away from consensus. This approach allows distributed systems to deal with unknown disruptions, without knowledge of the network topology or the numbers and behaviours of the disruptors, a general requirement of other resilient consensus algorithms. A disruptor-agnostic algorithm is particularly suitable to real-world applications where information regarding disruptors or network properties is typically unknown. Faster, tighter, and more robust convergence can be achieved across a range of scenarios with the social dynamics inspired algorithm presented herein, when compared with Mean-Subsequence-Reduced-type methods.

QUALITY AND SAFETY MANAGEMENT OF FERMENTED MILK PRODUCTS FROM CAMEL MILK BASED ON HACCP PRINCIPLES

Currently, the main task of domestic producers of milk and dairy products is to provide consumers with high-quality and safe dairy products. At the same time, the main guarantee of safe production is the introduction of the HACCP system at enterprises.The main advantage of the HACCP system is error detection, namely prevention and prevention, through step-by-step control of the entire food production chain.The research work was carried out on the basis of the national standard SS RK 1179-2003 «Quality systems. Food quality management based on the principles of HACCP. General requirements». At the first stage, the necessary information about the fermented milk product from camel milk was collected (composition, organoleptic characteristics, indicators characterizing quality and safety, application, type of packaging, storage and delivery conditions) and a flowchart of its production process was compiled. At the second stage, the results of scientific research by other authors and regulatory documents related to the quality and safety of camel milk and products from it were examined to analyze chemical, biological and physical risks. Then an assessment of the probability of the identified dangerous risks was carried out. Further, using the Decision Tree tool, critical control points in the technology of the research object were identified, allowing to manage safety and improve quality in their production.

Research on the Dilemma and Optimization Strategies of Industrial Development of Characteristic Towns under the Rural Revitalization Strategy

The report of the 20th National Congress of the Communist Party of China clearly pointed out that the most difficult and arduous task of building a socialist modern country in an all-round way is still in the countryside. As an important practical form of new urbanization, characteristic towns play a key role in the rural revitalization strategy. The construction of characteristic towns is a "complex ecosystem" integrating multiple fields such as industry, ecology, humanities, and space, which is highly consistent with the general requirements of the rural revitalization strategy. This paper takes Yuantan Brush Industry Town in Qianshan City, Anhui Province as an example to explore the difficulties and optimization strategies faced by its industrial development. Through literature review, field research and case analysis, the geographical and historical background, industrial development status and socio-economic conditions of Yuantan Brush Industry Town are first analyzed. The study found that Yuantan Brush Industry Town has obvious problems in terms of imperfect financial policies, unreasonable ecological zone division, insufficient incentives for professional talents and supporting policies. In response to these problems, this paper proposes a series of optimization strategies. First, enhance the degree of industrial agglomeration, promote cooperation between enterprises and the improvement of upstream and downstream industrial chains. Secondly, improve infrastructure, improve transportation conditions and living supporting facilities. Finally, increase policy support, ensure policy implementation and expand the scope of support. Through these measures, it is aimed to promote the sustainable development of Yuantan Brush Industry Town and achieve industrial upgrading and economic benefits. It is hoped that through the research in this article, experience can be provided for the development of characteristic towns in Anhui, the realization of the strategic goals of rural revitalization can be promoted, and the effective resolution and development of the "three rural" issues can be promoted.

The 3D plan analysis under progressive collapse for RC buildings through demand capacity ratio (DC) for three levels of damage

The progressive collapse presents a sudden load may cause initial failure in local parts at the frame which could lead to sequence reactions in the elements of the structure. A building of seven storeys was chosen to designed under gravity load according ACI 318 Code. This building is analyzed under progressive collapse condition for two columns damage cases; edge damage, corner damage. The building needs to be loaded as specified according (GSA) General Services Administration requirements through linear static analysis. The results of linear analysis show the variation in ((DC)) demand capacity ratio for the columns. The aspects of damage action in this study regarding section size and damage cases with 100% damage column loss (full damage), 60% damage (0.6 section size), 80% damage (0.8 section size). A GSA guideline to typical building with (DC) values bigger than 1.5 refer to critical potential damage conditions at the columns of the frame. It can be seen of the results the effect of size reduction for the building. This effect is clear to be decreased along distance from any plan. The((DC)) for columns which exceeded 1.5 value which show risky damage condition could actually happened that present serious threat possibility specially for nominated columns C22, C29 and C23 at plans A and B which show middle columns at lower position of the building.

Проблема анализа данных в культурно-историческом исследовании

<p>The article discusses the main ideas of the cultural-historical theory of social environment as a source of development from the point of view of the possibility of "refocusing research on development" and the transition from descriptive (empirical) ways of analysing experimental data to explanatory ones. In this article, based on Vygotsky's works, it is shown: 1) how the general idea of social environment as a source of development can be further conceptualised with the concepts of "social situation", "social situation of development" and <em>"perezhivanie"</em> and 2) how the analytical model created on this basis can become a concrete tool for analysing the experimental data. The cultural-historical genetic-analytical model developed by the author and experimentally validated is presented as a general framework for analysing experimental data in accordance with the general requirements of the experimental-genetic method. The second part of the paper presents a cultural-historical analytical matrix based on the model, which allows the data to be analysed in terms of revealing internal processes of development. The genetic-analytical model allows the analysis to capture the aspect of universality. A matrix based on this model (and therefore retaining the aspect of universality) can be used to analyse a particular social situation in terms of the specific conditions in which this universality manifests itself.</p>

Challenges and improvements in HER2 scoring and histologic evaluation: insights from a national proficiency testing scheme for breast cancer diagnosis in China

BackgroundIn 2022, our team launched the pioneering national proficiency testing (PT) scheme for the pathological diagnosis of breast cancer, rapidly establishing its credibility throughout China. Aiming to continuously monitor and improve the proficiency of Chinese pathologists in breast pathology, the second round of the PT scheme was initiated in 2023, which will expand the number of participating institutions, and will conduct a nationwide investigation into the interpretation of HER2 0, 1+, and 2+/FISH- categories in China.MethodsThe methodology employed in the current round of PT scheme closely mirrors that of the preceding cycle in 2022, which is designed and implemented according to the “Conformity assessment—General requirements for proficiency testing”(GB/T27043—2012/ISO/IEC 17043:2010). More importantly, we utilized a statistics-based method to generate assigned values to enhance their robustness and credibility.ResultsThe final PT results, published on the website of the National Quality Control Center for Cancer (http://117.133.40.88:3927), showed that all participants passed the testing. However, a few institutions demonstrated systemic biases in scoring HER2 0, 1+, and 2+/FISH- with accuracy levels below 59%, considered unsatisfactory. Especially, the concordance rate for HER2 0 cases was only 78.1%, indicating challenges in distinguishing HER2 0 from low HER2 expression. Meanwhile, areas for histologic type and grade interpretation improvement were also noted.ConclusionsOur PT scheme demonstrated high proficiency in diagnosing breast cancer in China. But it also identified systemic biases in scoring HER2 0, 1+, and 2+/FISH- at some institutions. More importantly, our study highlighted challenges in the evaluation at the extreme lower end of the HER2 staining spectrum, a crucial area for further research. Meanwhile, it also revealed the need for improvements in interpreting histologic types and grades. These findings strengthened the importance of robust quality assurance mechanisms, like the nationwide PT scheme conducted in this study, to maintain high diagnostic standards and identify areas requiring further training and enhancement.

Evaluating The Physical Quality of Trembesi Seedlings (Samanea saman) in The Permanent Nursery of BPDAS Indragiri Rokan, Pekanbaru City, Riau Province

The availability of high-quality seedlings is crucial for reforestation and forest rehabilitation initiatives aimed at mitigating deforestation. This study assesses the physical quality of rain tree (Samanea saman) seedlings produced by the Permanent Nursery of BPDAS Indragiri Rokan, located in Pekanbaru, Riau Province. A systematic sampling method with a random start was employed, involving 15,155 raintree seedlings that were six months old, from which a sample size of 200 seedlings was selected following SNI 8420 of 2018. The parameters observed included seedling height, stem diameter, leaf count (LCR), compactness of the planting medium, and root crown ratio. Data analysis was conducted based on the general and specific quality requirements outlined in SNI and Perdirjen RLPS No. P.05/V-Set/2009. The findings revealed that 66.5% of the seedlings satisfied the general requirements, while 72.38% met the specific requirements. The rain tree seedlings were classified within quality class D, with a percentage of normal seedlings falling below 95% and several samples exhibiting damage from pests and diseases. These results underscore the necessity for enhancements in nursery management, particularly concerning pest and disease control, to elevate the quality of raintree seedlings in future production.

Instrumental and legal nature of early release on parole from serving a sentence

The article is devoted to the study of the peculiarities of the content of early release on parole as a comprehensive criminal law remedy. It is stated that the functional purpose of conditional early release on parole from serving a sentence is to achieve the goals necessary for society. From the point of view of the instrumental theory of law, it is determined that early release on parole from serving a sentence constitutes a specific system of criminal law means by which the goals of criminal law regulation are achieved. According to the information and psychological orientation, such legal means are divided into stimulating and restrictive. It is found that legal facts, subjective rights, legitimate interests, benefits and incentives are the forms of manifestation of legal means-incentives. It is substantiated that legal incentives, directly orienting an individual to achieve beneficial consequences for him/her, are the most powerful incentive factor, and their application in the case of early release on parole from serving a sentence may be associated with both ordinary lawful behavior which does not exceed the general requirements and excessive performance of duties by a convicted person and is expressed in the form of elimination of restrictions determined by the criminal sentence applicable to a convicted person. The article emphasizes that legal remedies-restrictions are associated with unfavorable conditions for realization of interests of subjects, are focused on negative legal motivation and reduction of negative activity of subjects of law, and their forms of manifestation are legal facts, prohibitions, suspensions, legal obligations, penalties, etc. A legal restriction on early release on parole from serving a sentence is a prohibition in the form of a passive legal obligation of a convicted person not to commit any new criminal offense during the unexpired part of the previously imposed sentence. It is generalized that legal means-incentives and legal means-restrictions should be considered in an inseparable interrelation, since they are paired legal categories, i.e., internally dialectically interrelated and mutually supporting each other in the process of criminal law regulation. Thus, the author concludes that the instrumental and legal nature of early release on parole from serving a sentence as a complex criminal law tool is dialectically complex, since it combines directly opposite and at the same time internally unified criminal law means of influencing social relations.

Assuring fire safety in nuclear plants with international standards

This paper presents the evolution and benefits of international standards for fire safety engineering11Fire safety engineering and performance-based fire safety are both widely used, and both represent an engineering process to determine performance of a fire protection plan based on the results of fire calculations. developed at the International Organization of Standardization (ISO). The history of fire safety in nuclear power plants along with the need for fire safety regulation is discussed to lay the foundation for the standards development work. The evolution of fire safety regulation from prescriptive to performance-based is also presented as the background for the research.Extensive research of the issues that arise for performance-based fire safety regulation, specifically for computer models for fire safety calculations, which included international benchmark exercises formed the basis to identify the need for developing international standards for fire safety engineering. The research focused both on the verification and validation (V&V) of computer fire models and determining their predictive capability. The paper for the first time takes new scientific and engineering data from benchmark exercises to assess the suitability and applicability of computer fire models for application in nuclear power plants. The paper reports on the findings of the benchmark exercises and their use in formulating new requirements in ISO international standards on verification and validation of fire calculation methods and the fire safety engineering process through a consensus process of international experts.Discussion of the foundational research at the ISO fire safety engineering committee (ISO TC 92 SC 4) led to a consensus to revise two international standards. One specifying the principles and general requirements for fire safety engineering, and another on the verification and validation procedures for fire calculation methods. The development and content of these two international standards are discussed in this paper. An international technical report was subsequently developed at the ISO committee for conformity assessment (CASCO) to provide guidance for the certification of the fire safety engineering process, and specific parts of it, namely the V&V of fire calculation methods.It is concluded that the fire safety engineering international standards presented in this paper can support rigorous performance-based fire safety regulation for nuclear power plants. These ISO international standards will be particularly useful to nations that have not yet developed the regulatory infrastructure for nuclear power regulation. The international standards are also available to those that would like to transition to the use of international standards and an ISO standards-based certification scheme. Regulations based on these international standards will provide citizens with confidence in nuclear power as a viable option to address climate change.

A new method for joining Incoloy825/X65 composite plate by laser and CMT welding

Incoloy825/X65 composite plate had good application in the submarine transporting pipelines due to combination of the high resistance to Cl− corrosion and preferred mechanical properties. But the enrollment of Fe into Ni layer deteriorated the corrosion resistance in welding the transition layer of composite plate. In view of this, the paper respectively adopted the laser welding on the Ni layer without filler metal and then the swing arc CMT welding with steel filler metal as a transition in order to inhibit the Fe into Ni layer. The results showed the laser welding bead on Ni layer with uniform and fine γ microstructure ensured the better corrosion resistance. The CMT welding controlled the dilution rate under 6 % through optimizing the welding speed and swing frequency of arc. Finally, the tensile strength of transition weld was up to 500 MPa surpassing the base metal of 490 MPa. The clad bead with charge transfer resistance of 756 kΩ·cm2 satisfied general submarine pipeline anti-corrosion requirement.

Organisation of the Work of Bioanalytical Laboratories according to the Principles of Good Clinical Laboratory Practice (Review)

INTRODUCTION. Bioanalytical laboratories in the Russian Federation should follow the requirements of both the Good Laboratory Practices (GLP), when performing tests for non-clinical studies, and the Good Clinical Practices (GCP), when analysing samples from clinical studies. The work of such laboratories requires a separate GxP system, the Good Clinical Laboratory Practices (GCLP). However, the GCLP system has not yet been created in the Russian Federation and the Eurasian Economic Union (EAEU).АIM. This study aimed to compare the current Russian and EAEU principles regulating the work of bioanalytical laboratories with the international GCLP principles and formulate general requirements for national laboratories.DISCUSSION. The author analysed the current regulation of the work of bioanalytical laboratories, as well as the EAEU regulatory standards for the development and validation of analytical procedures. In addition, the study covered the international GCLP principles that govern the management of human resources, process record keeping, the development of standard operating procedures, the validation of analytical procedures, and the management of biological samples and reference standards in a laboratory. The author considered the key functions of a bioanalytical laboratory and suggested tools to manage them in compliance with the international GCLP principles and the EAEU requirements. It should be noted that scientific publications describe the international practice of applying the GCLP principles fairly well, and the experience of its implementation could be of use to Russian laboratories.CONCLUSION. A bioanalytical laboratory that implements the GCLP principles will increase its competitiveness in the EAEU market for bioanalytical testing services and mitigate its risks of obtaining invalid data.

Structural Reassignment of Covalent Organic Framework-Supported Palladium Species: Heterogenized Palladacycles as Efficient Catalysts for Sustainable C-H Activation.

Recent decades have witnessed remarkable progress in ligand-promoted C-H activation with palladium catalysts. While a number of transformations have been achieved with a fairly broad substrate scope, the general requirements for high palladium loadings and enormous challenges in catalyst recycling severely limit the practical applications of C-H activation methodologies in organic synthesis. Herein, we incorporate N,C-ligand-chelated palladacycles into rigid, porous, and crystalline covalent organic frameworks for the C-H arylation of indole and pyrrole derivatives. These heterogeneous palladium catalysts exhibit superior stability and recyclability compared to their homogeneous counterparts. We not only produce several highly reactive palladacycles embedded on new framework supports to facilitate C-H activation/C-C bond-forming reactions but also reassign heterogenized palladium species on frameworks containing a benzaldehyde-derived imine moiety as imine-based palladacycles via comprehensive characterization. Our findings provide guidance for the rational design of framework-supported metallacycles in the development of heterogeneous transition-metal catalysis.

Plant-Based Oils for Sustainable Lubrication Solutions—Review

Traditional lubricants, often containing harmful chemicals and synthetic or fossil-derived oils, pose environmental risks by damaging ecosystems and threatening human health and wildlife. There is a growing demand for environmentally sustainable and cost-effective bio-based lubricants derived from renewable raw materials. These bio-based oils often possess natural lubricating properties, making them an attractive alternative to traditional synthetic lubricants. In addition to providing effective lubrication, they offer good biodegradability and minimal toxicity, which are essential for reducing environmental impact. However, the primary challenge lies in optimizing their performance to match or surpass that of conventional lubricants while ensuring they remain cost-effective and widely available. This paper reviews the general requirements for lubricants and explores how plant-based oils can be utilized to meet the diverse lubrication needs across various industries. Further, it highlights different approaches that can be used for further improvements in the area of plant-based lubrication through bio-inspired means, such as the use of estolides, wax esters, or erucic acid, as well as through additions of nanomaterials, such as nanoparticles, nanoclays, or two-dimensional films.

REQUIREMENTS FOR A LEADER FROM THE POSITION OF STRATEGIC MANAGEMENT OF THE ENTERPRISE ON THE WAY TO SYSTEMIC PERFECTION

The article offers a list of requirements for a leader from the strategic management position of an organization that has chosen a course for systemic excellence. Leadership can be formal or informal. The head of the system is only sometimes the leader. The manager, as a leader, should meet specific requirements. Taking into account the historical experience of developing countries, which are still experiencing transformational processes with negative consequences, the question arises regarding the orientation of culture "as an image of life activity" of active elements - people, from the position of its condition in most subjects of socio-economic activity, about archetypes, in particular the archetype about the understanding of leadership, more in the practical plane, mainly as a formal attribute of power. Management subsystems of organizations in transitional/transformational conditions are not sufficiently focused on system criteria for choosing a leader. The article proposes general requirements for the system leader from the strategic management position, which has chosen a course for system excellence. The proposed list includes the following requirements: possess systemic and strategic thinking and be a carrier of progressive culture "as an image of life activity"; to be clearly aware of their purpose in the system; have high competence in managing complex systems; to clearly regulate one's job duties, powers and personal responsibility for achieving target strategic guidelines; to be a strategist and architect of the organization, especially in terms of defining and carrying out systemic strategic changes; to be a creative person and in every way to stimulate a creative approach to solving problems at all levels of management; to give priority to the goals of the system compared to personal goals and ambitions, to subordinate them to their purpose; have the image and reputation of a highly professional top manager; be oriented towards partnership both within the system itself and in the external environment (use the "win/win" strategy when the interests of all interacting parties are maximally satisfied). For the management subsystem, the list of requirements allows you to focus on the search, development, assessment of suitability, and replacement of the applicant for this role.

Adaptive ILC methods with less adaption parameters for non‐parameterized nonlinear continuous systems with nonsingular control gain matrices

SummaryIn this article, for non‐parameterized nonlinear continuous (NPNC) multiple‐input multiple‐output (MIMO) systems, two combined iteration‐domain and time‐domain adaptive iterative learning control (ILC) algorithms are proposed to track iteration‐varying reference trajectories repetitively over a finite time interval. Different from the general requirement in adaptive control community that the control gain matrices of the controlled systems are real symmetric and positive‐definite, only the nonsingular property of the control gain matrices is assumed. Moreover, there are just two adaption parameters and one adaption parameter involved in the proposed two adaptive ILC algorithms respectively such that the computation load and memory‐space are greatly saved. A simulation example is utilized to illustrate the effectiveness of the two proposed adaptive ILC algorithms with less adaption parameters.

Noncontact measurement of rectangular splines shaft based on line-structured light

Abstract The rectangular spline shaft, a typical type of shaft-tooth component, plays a significant role in mechanical transmissions. Existing methods for detecting size and positional tolerance in spline shafts often rely on contact-based measurement techniques, including specialized gauges and coordinate measuring machines (CMMs). To enhance the measurement efficiency, this paper proposes a method for measuring based on line-structured light. Firstly, a classification algorithm for data points on major and minor cylindrical surfaces and keyway surface of spline shaft is established, contributing to the automatic measurement. Secondly, a coaxiality error measurement model is established based on the overall least squares method, improving the measurement accuracy. Finally, a measurement model for key width and positional tolerance is established through the rotation of the spline axis. In experiments, the size and positional tolerance of the spline shaft obtained using this method are compared with measurements by CMM, meeting the general machining accuracy requirements.

Visualizing DNA/RNA, Proteins, and Small Molecule Metabolites within Live Cells.

Live cell imaging is essential for obtaining spatial and temporal insights into dynamic molecular events within heterogeneous individual cells, in situ intracellular networks, and in vivo organisms. Molecular tracking in live cells is also a critical and general requirement for studying dynamic physiological processes in cell biology, cancer, developmental biology, and neuroscience. Alongside this context, this review provides a comprehensive overview of recent research progress in live-cell imaging of RNAs, DNAs, proteins, and small-molecule metabolites, as well as their applications in molecular diagnosis, immunodiagnosis, and biochemical diagnosis. A series of advanced live-cell imaging techniques have been introduced and summarized, including high-precision live-cell imaging, high-resolution imaging, low-abundance imaging, multidimensional imaging, multipath imaging, rapid imaging, and computationally driven live-cell imaging methods, all of which offer valuable insights for disease prevention, diagnosis, and treatment. This review article also addresses the current challenges, potential solutions, and future development prospects in this field.