PURPOSE: The objective of this study was to investigate whether MyPlate, used as a nutritional assessment and an educational platform, can improve nutritional knowledge, eating attitudes and physical activity levels compared to food records. METHODS: One hundred twenty university students (18-22 years old) with a normal Body Mass Index (BMI) were recruited and randomly yet equally assigned into one of three groups after signing an IRB approved informed. The groups were: MyPlate group (MG), food records group (FG), and control group (CG). All participants attended two nutritional seminars and completed a modified and validated general nutrition knowledge questionnaire (GNKQ), Eating attitude test (EAT-26) at the beginning and the end of the 4-week study period. A detailed exercise log was also obtained from each participant for the entire period of study. RESULTS: Participants in the MG group showed a significant improvements in their nutritional knowledge (p<0.05), eating attitude (p<0.05) and physical activity (p<0.05) compared with other groups. Vegetable consumption elevated from start to finish in the MG (p<0.05) along with a trend towards elevated wholegrain consumption. Males tended to show a more positive eating behavior compared with females in all groups. No significant differences were observed in eating attitude, and physical activity in FG compared with CG. CONCLUSION: MyPlate appears to be an effective tool to improve physical activity, nutritional knowledge and promote positive eating behaviors and therefore wellness.