A new species, Dilophochila glabra sp. nov. is described from the Sierra Norte, Oaxaca, Mexico. Diagnosis and images (dorsal, lateral and ventral habitus, and genitalia) of the taxonomic characters are provided. Affinities with their closest congeners and the general distribution pattern of the genus are discussed, considering that it presents a typical Mountain Paleoamerican sub-pattern. The modifications in the mouthparts of this genus due to the specialization in the intake of pine needles are commented, as well as in two other different taxa within Anomalini.