Cardiovascular disease (CVD) is ranked highest among non-communicable diseases and considered as main cause of deaths all over the world. Genetic variants of various genes have been reported strongly associated with risk of CVDs. KCNQ1 gene which has been found to be linked with diabetes mellitus, also plays an important role in cardiac functioning. Genetic variation in KCNQ1 gene can affect the normal functioning of heart. Therefore, this study was conducted to evaluate the association between KCNQ1 gene variant rs2237892: C > T and CVD. The genotyping of genetic variant rs2237892: C > T of KCNQ1 gene was done in 100 angiography-proven CVD patients and 91 healthy controls by using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) method. The distribution of genotypes was found significantly different in both case and control subjects (p < 0.05). The CC genotype of rs2237892 SNP was found to be correlated with the increased level of Triglycerides (TG) in CVD cases (p- value- 0.007). Additionally, genetic model (CC vs. CT + TT) reveals a link between the C allele and the risk of cardiovascular disease (OR – 3.671, CI: 2.012–6.696, p-value – <0.001). The present study concludes rs2237892: C > T genetic variant, C allele as the pre-disposing factor for development of CVD in the studied population of India.