Menstruation remains surrounded by taboo and symbolic violence for many adolescents and young women today, and it is urgent to identify existing stereotypes. The objective was to validate a Spanish-language instrument to assess perceptions, false beliefs, gender stereotypes, fears, and taboos surrounding menstruation in nursing women's studies. A cross-sectional study for psychometric validation of the METCON© scale (22 item version) was assessed in a cohort of 401 female nursing university students (2016-2019), assessing the psychometric properties in terms of internal consistency and structural validity. Furthermore, an invariance analysis was conducted to discern structural disparities before and after studying the subject of gender and health. The METCON© scale showed acceptable internal consistency scores measured by Cronbach's alpha. Exploratory factor analysis indicated a structure of six factors which explained 60.50% of the total variance with a total of 19 items. Reliability analysis indicated adequate internal consistency (α = 0.613). Confirmatory factor analysis showed adequate values, confirming this structure. The invariance analyses identified that the structure remained adequate when evaluated before and after studying the subject of gender and health. Once validated, this instrument could serve as a valid and reliable tool for comprehensively examining perceptions, beliefs, and myths surrounding menstruation, addressing not only biological but also social and cultural aspects.