Burnable absorbers play an important role in nuclear reactor safety and exploring their effect on the reactor core behavior is an important issue in the design and operation of the reactor core. The aim of the present work was to discover the effect of burnable absorbers on criticality and reactivity coefficient of VVER-1000 assembly. The calculations were conducted with a 3-D Monte Carlo transport code MCNP6 and ENDF/B-VII.1 nuclear library. The Gd2O3 content varying from 0 wt.% to 8 wt.% was considered to complete the inter-comparison analysis between the criticality and the reactivity coefficients for three UO2+Gd2O3 fuel configurations. At the beginning of the cycle, there is a significant difference between the criticality (kinf) of assembly with and without Gd2O3, however, at the middle of the cycle those differences become very small and almost the same at the end of the cycle. The Doppler temperature coefficient values are always sufficiently negative and demonstrate a more negative trend with increasing gadolinium concentrations and fuel burnup. At beginning of the cycle, the moderator temperature coefficient value increases negatively as gadolinium concentration increases but, at middle of the cycle this trend does not occur. The fuel composition is predicted to be the reason behind this situation. At the end of cycle, there is no clear trend in the moderator temperature coefficient values with respect to Gd2O3 concentration. The absorbing effect of Gd2O3 appears to have diminished significantly. Overall, this research provides insights into the influence of the burnable absorbers on the neutronic parameters of the VVER-1000 assembly and its contribution to reactor safety.