Objectives: Data on the antimicrobial susceptibility profiles and the frequency of the scpB and lmb virulence genes among Streptococcus agalactiae (GBS) from pregnant women could develop a strategic for preventing neonatal GBS infections. This study was aimed (1) To determine the antibiotic susceptibility of GBS strains isolated from the pregnant women; and (2) To determine the scpB and lmb virulence genes among isolated GBS strains. Materials and method: 24 GBS strains were subjected to the antibiotic susceptibility testing (AST) by the disc diffusion (Kirby-Bauer) method. The virulence genes (scpB and lmb) were detected by using PCR method. Results: All 24 GBS were susceptible to penicillin, cefotaxime, vancomycin and clindamycin. A large proportion of GBS isolates were found to be resistant to erythromycin (75%) and tetracycline (66.7%). The resistance rates for levofloxacin and chloramphenicol were 54.2% and 20.8%, respectively. The scpB gene was present in 95.8% of these GBS isolates and the lmb in 91.7% of isolates. Conclusion: Penicillin is still the antibiotic of the first choice for GBS infection treatment in Hue Medicаl University Hospitаl; clindamycin or vancomycin could be used for penicillin-allergic patients. Erythromycin should be prescribed with caution. Most of the strains were positive for scpB and lmb genes. Key words: Streptococcus agalactiae (GBS), virulence gene, antimicrobial susceptibility profiles, pregnant woman