Multi-focal image and multi-modal image fusion technology can fully take advantage of different sensors or different times, retaining the image feature information and improving the image quality. A multi-source image fusion algorithm based on bidimensional empirical mode decomposition (BEMD) and a region sharpness-guided region overlapping algorithm are studied in this article. Firstly, source images are decomposed into multi-layer bidimensional intrinsic mode functions (BIMFs) and residuals from high-frequency layer to low-frequency layer by BEMD. Gaussian bidimensional intrinsic mode functions (GBIMFs) are obtained by applying Gaussian filtering operated on BIMF and calculating the sharpness value of segmented regions using an improved weighted operator based on the Tenengrad function, which is the key to comparison selection and fusion. Then, the GBIMFs and residuals selected by sharpness comparison strategy are fused by the region overlapping method, and the stacked layers are weighted to construct the final fusion image. Finally, based on qualitative evaluation and quantitative evaluation indicators, the proposed algorithm is compared with six typical image fusion algorithms. The comparison results show that the proposed algorithm can effectively capture the feature information of images in different states and reduce the redundant information.