Gastritis Patients Research Articles

The correlation of serum sirt6 with clinical outcome and prognosis in patients with gastric cancer

We aimed to evaluate the correlation between serum sirtuin 6 (sirt6) level and clinicopathological characteristics and prognosis of gastric cancer (GC) patients. The serum sirt6 levels of subjects (135 cases of GC, 68 cases of atrophic gastritis, 60 cases of healthy controls) were analyzed by enzyme-linked immunosorbent assay. The predictive and prognostic values of sirt6 serum level for GC were determined by performing receiver operating characteristic curve (ROC), Kaplan-Meier analysis, as well as univariate and multivariate Cox regression, respectively. GC patients showed lower sirt6 serum levels than that of atrophic gastritis patients and healthy control. Taking the healthy control as a reference, the area under the ROC curve (AUC) of sirt6 serum level for diagnosing GC was 0.955 with a sensitivity of 91.85% and a specificity of 90.0%. Based on ROC analysis using atrophic gastritis as the state variable, serum sirt6 had a high diagnostic efficiency for GC (AUC = 0.754). Serum sirt6 was related to the clinicopathological features (tumor size, Lauren's classification, tumor node metastasis staging, lymph node metastasis) and overall survival (log-rank χ2 = 12.22, P < .001). The AUC of serum sirt6 predicting death in GC patients was 0.731. At the optimal cutoff value (16.83 ng/mL), the sensitivity and specificity of sirt6 were 59.57% and 79.55%, respectively. Moreover, lower sirt6 level as independent risk factor was revealed to affect prognosis of GC patients (P = .018). Serum sirt6 level was positively associated with the tumor stage and metastasis conditions, which could be served as diagnostic and predictive biomarkers in GC.

Helicobacter pylori virulent genes and host interleukin 1 are associated with demographic factors for an increased risk of gastritis

AimHelicobacter pylori is a predominant pathogen for the human race and causes gastrointestinal diseases as well as can be an increased risk for cancer. This study examined the bacterial and host genes involved in gastritis and the environmental influences to understand the disease outcome. MethodsPolymerase chain reaction assay was used to analyze the variations in virulence-associated genes of H. pylori in gastritis patients. Human interleukin 1 beta gene polymorphisms and IL-1 receptor antagonist gene (IL-1RN intron 2) variable number tandem repeats were evaluated by PCR and single-strand conformation polymorphism analysis, respectively. Various demographic factors were analyzed and all the statistical tests were two-sided. ResultsInfection with cagA, cagA – Asian lineage positive strains of H. pylori was associated with an increased risk for gastritis. IL-1B-511*T carriers homozygous for the short allele of IL-1RN (IL-1RN*2/*2) had an increased risk for gastritis for the cagA carrier. The odd ratio (OR – 0.17; 95% CI – 0.03 to 0.08; P = 0.04) was significant in certain bacterial (cagA) and host (IL-1RN*2) high-risk genotypes in the gastritis patients. ConclusionThe pathogen and the host genetics in relation to lifestyle habits provide a tool for defining the high-risk individuals and strategize H. pylori eradication programs.

Trace element levels in serum and gastric mucosa in patients with Helicobacter pylori positive and negative gastritis

BackgroundMost trace elements are inhibited by Helicobacter pylori-infection, and variations in specific element levels are linked to the development of stomach cancer. This is the first study to show the relationship between serum and tissue concentrations of twenty-five trace elements and H. pylori infection status. This study purposed to define serum and tissue trace element levels of 25 healthy individuals with Helicobacter pylori-positive gastritis and Helicobacter pylori-negative gastritis and to reveal their relationship with the disease. MethodsStudy groups consisted of sixty-two patients with Helicobacter pylori-positive, thirty-seven patients with Helicobacter pylori-negative, and thirty healthy individuals. Serum and tissue concentrations of twenty-five elements (aluminum, boron, arsenic, barium, calcium, beryllium, copper, cadmium, iron, chromium, mercury, lithium, potassium, magnesium, sodium, manganese, nickel, phosphorus, lead, scandium, strontium, selenium, tellurium, titanium, zinc) were defined by inductively coupled plasma optical emission spectrometry. ResultsExcept for copper, lithium, and strontium elements in serum samples, other trace elements differed significantly between the groups (p < 0.05). The serum chromium (p = 0.002), mercury (p = 0.001), boron (p < 0.001), and cadmium (p < 0.001) levels of H. pylori-negative gastritis and H. pylori-positive gastritis participants were significantly different, and their serum concentrations were less than 0.5 µ/l. Boron, barium, beryllium, chromium, lithium, phosphorus and strontium elements in tissue samples did not differ significantly between the groups (p > 0.05). Manganese, nickel, tellurium and titanium elements were not detected in tissue and serum samples. The mean concentrations of calcium, beryllium, chromium, iron, potassium, lithium, magnesium, scandium, and selenium were higher in the tissues of patients with H. pylori gastritis compared to healthy control tissues. Also, cadmium could not be detected in tissue samples. There was a significant difference between H. pylori-infected tissue and serum chromium levels (p = 0.001), with lower levels detected in tissue samples. ConclusionThis is the first study that we are knowledgeable of that reports the concentrations of twenty five elements in both serum and tissue samples, as well as the relationship between trace elements and Helicobacter pylori-infection status. Dietary adjustment is indicated as an adjunct to medical therapy to stabilize trace elements because Helicobacter pylori bacteria cause inflammation and impair element absorption in gastritis patients. We also think that this study will shed light on studies on the relationship between Helicobacter pylori-trace elements and serum-tissue/healthy serum-tissue trace element levels of patients with Helicobacter pylori gastritis.

Characteristics of basal gastric juice in Helicobacter pylori-associated gastritis before and after eradication therapy

Purpose: To evaluate the characteristics of basal gastric juice in Helicobacter pylori-positive patients before and after Helicobacter pylori eradication therapy. &#x0D; Methods: This was a cross-sectional descriptive study on 150 gastritis patients admitted at the Hospital of Can Tho University of Medicine and Pharmacy. The patients were divided into 2 groups: study group (Helicobacter pylori gastritis patients) and control group (non-Helicobacter pylori gastritis patients). The pH, HCO3- concentration, and activities and concentrations of pepsin, lipase, and amylase were determined before and after treatment in study group. &#x0D; Results: Patients with abnormal gastric juice comprised 76 % of the study population. Mean gastric potential of hydrogen (pH) was 2.31 (range: 1.64 - 7.68), while median concentration of HCO3- was 4.06 mmol/L (range: 0 - 73.04 mmol/L). The concentrations of pepsin, lipase, and amylase were 8.93, 0.93 and 1.38 ppm, respectively. Activities of pepsin, lipase, and amylase were 2.23, 0.28 and 0.04 U/mL, respectively. After the successful eradication of Helicobacter pylori, pH and HCO3- levels decreased, and there were significant differences in activities of pepsin and lipase before and after treatment (p &lt; 0.05). Moreover, the levels of these parameters differed between patients in whom successful eradication was achieved and those in whom eradication failed (p &lt; 0.05). The concentrations and activities of pepsin and lipase were statistically different between pre-treatment and post-treatment stages in both successful and failed Helicobacter pylori eradication categories (p &lt; 0.05). &#x0D; Conclusion: Basal gastric juice differs significantly between Helicobacter pylori-positive and Helicobacter pylori-negative patients. Intragastric ammonia produced by H. pylori may have a role in the increased pH of gastric juice.

A Case Report of Chronic Gastritis in a Patient with Spinal Stenosis Hospitalized at a Korean Medicine Hospital and Treated with Korean Medicine

Objectives: The purpose of this study is to report the efficacy of applying Korean medical treatment to chronic gastritis.Methods: We treated a chronic gastritis patient with Korean medical treatments (herbal medicines, acupuncture, and herbal acupuncture therapy). To evaluate the results of this treatment, we took measurements using the Gastrointestinal Symptom Rating Scale and Visual Analogue Scale for abdominal pain, heartburn, nausea, and abdominal distension. The patient’s generic health status was evaluated using the European Quality of Life 5 Dimensions Scale.Results: Improvements in gastrointestinal symptom ratings and the European Quality of Life 5 Dimensions Scale were observed after the treatments. Relief of symptoms was observed in abdominal pain, heartburn, nausea, vomiting, and abdominal distension using the Visual Analogue Scale.Conclusions: This study suggests that Korean medicine may be effective in the treatment of chronic gastritis.

Benson Therapy in Gastritis Patients to Reduce Pain

Gastritis is an inflammation of the gastric mucosa caused by an irregular diet. Gastritis can be an emergency if not treated immediately, even in some conditions gastritis can also cause perforation to death. This study aims to obtain an overview of benson relaxation interventions to reduce pain in gastritis clients. The writing used uses a descriptive case study method through a nursing process approach that includes assessment, nursing diagnosis, planning, implementation and evaluation. With gastritis patient participants with acute pain problems in the Dahlia Room BLUD RSU Kota Banjar. Data collection through interviews, observation, physical examination, and documentation. Based on the results of the pain scale assessment, the results of the original scale reduction from 5 (0-10) to 2 (0-10) using the Numeric Rating Scale (NRS) measuring instrument method. The author found an effect after giving benson relaxation intervention on reducing pain intensity in gastritis patients and getting effective results, as evidenced by a decrease in the intensity of the pain scale. From the case study of benson relaxation intervention can be an alternative intervention that is done to gastritis patients so that there are no complications from the disease.

Targeted metabolomic profiles of serum amino acids and acylcarnitines related to gastric cancer.

BackgroundEarly diagnosis and treatment are imperative for improving survival in gastric cancer (GC). This work aimed to assess the ability of human serum amino acid and acylcarnitine profiles in distinguishing GC cases from atrophic gastritis (AG) and control superficial gastritis (SG) patients.MethodsSixty-nine GC, seventy-four AG and seventy-two SG control patients treated from May 2018 to May 2019 in Gansu Provincial Hospitalwere included. The levels of 42 serum metabolites in the GC, AG and SG groups were detected by liquid chromatography-tandem mass spectrometry (LC–MS/MS). Then, orthogonal partial least squares discriminant analysis (OPLS-DA) and the Kruskal-Wallis H test were used to identify a metabolomic signature among the three groups. Metabolites with highest significance were examined for further validation. Receiver operating characteristic (ROC) curve analysis was carried out for evaluating diagnostic utility.ResultsThe metabolomic analysis found adipylcarnitine (C6DC), 3-hydroxy-hexadecanoylcarnitine (C16OH), hexanoylcarnitine (C6), free carnitine (C0) and arginine (ARG) were differentially expressed (all VIP >1) and could distinguish GC patients from AG and SG cases. In comparison with the AG and SG groups, GC cases had significantly higher C6DC, C16OH, C6, C0 and ARG amounts. Jointly quantitating these five metabolites had specificity and sensitivity in GC diagnosis of 98.55% and 99.32%, respectively, with an area under the ROC curve (AUC) of 0.9977.ConclusionThis study indicates C6DC, C16OH, C6, C0 and ARG could effectively differentiate GC cases from AG and SG patients, and may jointly serve as a valuable circulating multi-marker panel for GC detection.

Features of the cytokine status in patients with HP-negative chronic gastritis

Aim: to study the immunological characteristics of patients with H. pylori-unassociated chronic gastritis with its comorbidity with allergic rhinitis.Materials and methods. The study included patients aged 18 to 40 years: 47 healthy individuals (comparison group) and 140 people, of which 66 people had chronic gastritis, 43 people had seasonal allergic rhinitis in remission, 31 people had a combination of both. Conducted a clinical examination, determination of serum concentrations of IL-4, IL-5, IL-6, IL-8, IL-10, IL-18, MCP-1, total IgE, detection of IgG to the nuclear antigen of the Epstein-Barr virus.Results: During the study, we showed that H. pylori-non-associated chronic gastritis is characterized by a decrease in the level of monocyte-macrophage cytokines (IL-6 and IL-18, but not IL-8), but an increase in the production of Th2-dependent cytokines (IL-5 and IL-4), as well as total Ig E. In addition, H. pylori-non-associated chronic gastritis in patients without allergic rhinitis is associated with an increased frequency of detection of IgG to the nuclear antigen of the Epstein-Barr virus in blood serum (80.0% vs 48–55% of cases in other groups, p&lt;0, 05).Conclusion. Thus, H. pylori-non-associated chronic gastritis is a multidisciplinary problem, which, from an immunopathogenetic point of view, is characterized by a Th2-phenotype of the immune response in such patients, including in the absence of allergic pathology, which determines the need for a wider involvement of data in the examination. patients of specialists of other profiles (allergists, infectious disease specialists, etc.).

S3664 Russel Body Gastritis: When the Rare Meets the Odd

Introduction: The significance of chronic gastritis is underrated. Even though Helicobacter pylori (HP) has been the main contributing factor to this condition, if testing for it comes back negative, we should always rule out other underlying diseases, especially if the biopsy reveals Russell body (RB) gastritis. Case Description/Methods: A 67-year-old male with a past medical history of immune thrombocytopenic purpura (ITP) on prednisone and eltrombopag, hypertension, and choledocholithiasis status post cholecystectomy complicated by abscess formation and drain placement, presented with worsening nausea, vomiting, and abdominal pain for three days associated with early satiety and weight loss. His symptoms were not related to food or activity. Vital signs and physical exam were unremarkable except for right upper quadrant abdominal tenderness. Labs were remarkable for WBC 55 k/ul, HGB 11 g/dl, and PLT 90 k/ul. Full infectious workup was negative. CT scan showed massive splenomegaly with widespread lymphadenopathy and circumferential wall thickening of the gastric fundus and body. An upper endoscopy was performed and revealed diffuse gastric erythema in a mosaic pattern, most pronounced in the body of the stomach (Fig. 1a). Biopsies were obtained from the gastric antrum and body. Gastric body biopsies revealed gastric oxyntic mucosa with RB gastritis (Fig. 1b), and it was negative for HP, intestinal metaplasia, dysplasia, or malignancy. Kappa and lambda immunohistochemistry stain showed a polyclonal mixed population of plasma cells (Fig. 1c), indicating no underlying malignancy. HP stool antigen was checked twice, and it was negative. His WBC continued to rise, and bone marrow (BM) biopsy was performed. Findings were suspicious for myelodysplastic/myeloproliferative disorder; however, a reactive process could not be ruled out. Since the patient's condition significantly deteriorated, a repeat BM biopsy revealed morphological changes consistent with chronic myelomonocytic leukemia (CMML). After discussing goals of care with the patient, he opted for hospice care and expired after three days.Figure 1.: (a) An upper endoscopy showing diffuse punctuate gastric erythema with mucosal mosaic pattern. (b) A high power stomach biopsy with Hematoxylin and Eosin stain showing atypical plasma cells with Russell bodies (yellow arrow) which are eosinophilic homogeneous immunoglobulin-containing inclusions. (c) A high power stomach biopsy with kappa and lambda immunohistochemistry stain showing polyclonal mixed population of plasma cells (positive for both kappa and lambda). Discussion: RB gastritis is a reactive mucosal infiltration of plasma cells filled with cytoplasmic RB. There is a strong association with HP infection, but there is also an association with hematopoietic malignancies and gastric adenocarcinoma. Our case represents the vital importance of looking for underlying malignancies in HP negative RB gastritis patients.

Comparison of the Efficacy of Conventional Versus Sequential Regimen in Patients of Gastritis Presenting with Helicobacter Pylori

Objective: To compare the efficacy of conventional versus sequential regimen in patients of gastritis presenting with Helicobacter pylori. Study Design: Randomized Controlled Trial Setting: Unit I, Department of Gastroenterology, Lahore General Hospital, Lahore Duration of Study: Six months after the approval of synopsis i.e. 30th November 2017 to 30th May 2018. Methodology: 232 patients fulfilled inclusion criteria were enrolled in the study. Patients were randomly divided in two equal groups. In group A, conventional triple treatment was given. In group B, sequential therapy was given. After 30 days, urea breath test was done. Reports were assessed and if there will be no bacterium, and then efficacy was labeled. All this information was recorded through proforma. Data analysis was done using SPSS version 21. Results: The mean age of patients was 38.63±13.11year in conventional group and 36.46±12.16years in sequential group. In conventional group, there were 88 (75.9%) male and 28 (24.1%) females. In sequential group, there were 74 (63.8%) male and 42 (36.2%) females. With conventional therapy, efficacy was achieved in 82 (70.7%) patients while with sequential therapy, efficacy was achieved in 100 (86.2%) patients. The difference was significant (p&lt;0.05). Conclusion: Thus the sequential therapy is more effective than conventional therapy for eradication of H. pylori in patients of gastritis. Keywords: Gastritis, H. pylori, conventional therapy, sequential regimen

Analysis of the relation between elevated neutrophil lymphocyte ratio, and erythrocyte sedimentation rate in Helicobacter pylori positive chronic gastritis patients

Background: Aim of the study was to analyse the relation between elevated neutrophil lymphocyte ratio (NLR), and erythrocyte sedimentation rate (ESR) in Helicobacter pylori (H. pylori) positive chronic gastritis patients, as compared to the control group containing H. pylori negative chronic gastritis patients.Methods: Chronic gastritis patients were segregated in equal numbers based on H. pylori status. NLR was calculated, and ESR noted from the observations, comparison was done between the control and the study groups.Results: A total of 100 patients were included in the study. The 50 each from the control and study group. An observation of elevation in NLR and ESR in H. pylori positive chronic gastritis patients, as compared to the control group was seen. With an average NLR of 2.43 and 1.43, in the control and study group, respectively.Conclusions: Raise in NLR in H. pylori positive chronic gastritis patients with an associated raise in ESR suggests, the severity of the infection and the need for eradication and prevent complications.

Lemon Aromatherapy Intervention to Reduce Nausea and Vomiting in Gastritis Patients

Objective: To present care with lemon aromatherapy intervention as an effort to overcome nausea and vomiting in gastritis patients. Methods: The method used is descriptive and uses nursing care that refers to the IDHS and SIKI books. The subject of this study was 1 patient with a nursing diagnosis of nausea and vomiting in gastritis patients who were treated in the Dahlia Room, Banjar City Hospital. Data collection includes interviews, observations, physical examinations and documentation. Results: The results of the case studies conducted showed a change after the non-pharmacological technique of lemon aromatherapy was proven by the final results, namely the evaluation carried out by the patient not feeling nauseated, the INVR scale 3. Conclusion: The conclusion from the results of the lemon aromatherapy intervention was considered effective for reducing nausea and vomiting with a decreased INVR scale. with the results of the final evaluation of the INVR 3 scale, the client said that nausea was reduced.

Comprehensive Evaluation of Serum tRF-17-WS7K092 as a Promising Biomarker for the Diagnosis of Gastric Cancer.

Background Gastric cancer (GC) is a malignant tumor of the gastrointestinal system. Since the early symptoms of GC are not obvious and lack efficient diagnostic markers, it is urgent to find new diagnostic markers with good sensitivity and specificity. tRNA-derived small RNAs (tsRNAs) are an emerging class of small noncoding RNAs with good abundance in body fluids. We aim to find new tsRNAs as biomarkers for GC diagnosis. Methods High-throughput sequencing was used to identify differentially expressed tsRNAs in GC tissues, and quantitative real-time PCR was used to detect the expression level of tRF-17-WS7K092. Agarose gel electrophoresis and Sanger sequencing were performed to verify the characteristics of tRF-17-WS7K092. The diagnostic efficacy of tRF-17-WS7K092 was analyzed by the receiver operating characteristic curve. Results In this study, the expression levels of tRF-17-WS7K092 were significantly increased in GC tissues, cells, and serum. After GC surgery, the expression level of serum tRF-17-WS7K092 decreased, and its high expression was associated with low survival rates. In addition, the expression level of serum tRF-17-WS7K092 was correlated with the T stage, TNM stage, lymph node metastasis, and nerve/vascular invasion and could distinguish GC patients from gastritis patients and healthy donors as well. Conclusions The expression of serum tRF-17-WS7K092 was significantly increased in GC and decreased after GC surgery, suggesting that serum tRF-17-WS7K092 may serve as a promising biomarker for the diagnostic and prognostic monitoring of GC.

How is the Lifestyle of Gastritis Patients?

Lifestyle is showing how people live, how to spend money, and how to allocate time. The purpose of this study is to describe the lifestyle of gastritis patients in Banjar Pemijian Sangeh, Abiansemal District, Badung Regency in 2021. This research is a descriptive study with a quantitative design. The approach used is approach cross sectional. The sampling method in this study is Probability Sampling with simple Random Sampling. The research was conducted in February-April 2021 in Banjar Pemijian Sangeh with a total sample of 39 respondents. The results of this study in terms of the characteristics of the respondents, it was found that the average age of those suffering from gastritis from 16-55 years was 26.59 years, with 17 respondents (43.6%) of undergraduate education, and the majority working as private employees as many as 16 people (41.4%). Based on the results of the lifestyle research on gastritis patients who have good eating and drinking habits as many as 31 people (79.50%), and 9 people (23.10%) have sufficient smoking behavior, and good physical activity respondents at the age of 16 -25 years as many as 31 people (79.50), and respondents with a good stress level with 79.50% were 31 people. For gastritis patients, it is hoped that a healthy and good lifestyle can prevent the onset of gastritis.

Long noncoding RNA ZNFX1-AS1 promotes the invasion and proliferation of gastric cancer cells by regulating LIN28 and CAPR1N1.

BACKGROUNDLong noncoding RNA (lncRNA) ZNFX1-AS1 (ZFAS1) is a newly discovered lncRNA, but its diagnostic value in gastric cancer is unclear.AIMTo investigate the potential role of ZFAS1 in gastric cancer and to evaluate the clinical significance of ZFAS1 as a biomarker for gastric cancer screening.METHODSQuantitative real-time polymerase chain reaction (qRT-PCR) was used to screen for gastric cancer-associated lncRNAs in gastric cancer patients, gastric stromal tumor patients, gastritis or gastric ulcer patients, and healthy controls. Correlations between ZFAS1 expression and clinicopathological features were analyzed. The biological effects of ZFAS1 on the proliferation, migration, and invasion of gastric cancer cells were studied by MTT, colony formation, and transwell mi-gration assays. The potential mechanism of ZFAS1 was demonstrated using enzyme-linked immunosorbent assay and qRT-PCR. The relationship between ZFAS1 and tumorigenesis was demonstrated using in vivo tumor formation assays.RESULTSThe plasma level of lncRNA ZFAS1 was significantly higher in preoperative patients with gastric cancer than in individuals in the other 4 groups. Increased expression of ZFAS1 was significantly associated with lymph node metastasis, advanced TNM stage, and poor prognosis. ZFAS1 regulated the proliferation, migration, and invasion of gastric cancer cells and regulated the growth of gastric cancer cells in vivo. LIN28 and CAPRIN1 were identified as key downstream mediators of ZFAS1 in gastric cancer cells.CONCLUSIONLncRNA ZFAS1 promoted the invasion and proliferation of gastric cancer cells by modulating LIN28 and CAPRIN1 expression, suggesting that ZFAS1 can be used as a potential diagnostic and prognostic biomarker in gastric cancer.

POTENSI INTERAKSI OBAT PADA PASIEN GASTRITIS DI PUSKESMAS RAWAT INAP WAY KANDIS BANDAR LAMPUNG TAHUN 2020

ABSTRACT Gastritis is an inflammatory process in the mucous and sub-mucous layers of the stomach. The use of several drugs simultaneously facilitates the occurrence of drug interactions that are part of the Drug Related Problem (DRPs). This study aims to determine the potential for drug interactions in gastritis patients at the Puskesmas Rawat Inap Way Kandis Bandar Lampung in 2020. The research method carried out is observational research with a descriptive design taken retrospectively. The results of the study based on gender showed that there were 34 male patients (40.5%) and 50 female patients (59.5%). Then, based on the age group of 17-25 years, there were 26 patients (31%), aged 26-35 years as many as 16 patients (19%), aged 36-45 as many as 19 patients (22.6%), aged 46-55 as many as 13 patients (15.5%), aged 56-65 as many as 5 patients (6%), and aged &gt;65 years as many as 5 patients (6%). The most use of gastritis drugs are antacids (92.8%), omeprazole (21.4%), ranitidine (15.1%). Drug interactions based on pharmacokinetic mechanisms were 20 cases (58.8%), and pharmacodynamic mechanisms were 14 cases (41.1%). Severity, 18 cases (52.9%) minor, 14 cases (41.1%) moderate, and 2 cases (5.8%) major. It was concluded that the potential drug interactions that experienced the incidence of drug interactions were 27 samples (32.1%) and 57 samples (67.8%) did not experience drug interactions. Â Keywords : Antacids, DRPs, Drug Interactions, Gastritis, Omeprazole, Ranitidine

DNMT1 Gene Expression in Patients with Helicobacter pylori Infection.

DNMT1, as a critical enzyme affecting epigenetics through methylation of DNA cytosine-rich sequences, regulates gene expression. Exterior factors including long-term infections, in this study Helicobacter pylori infection, could change host cells' epigenetics by affecting DNMT1 gene expression. This study investigated the statistical correlation between H. pylori virulence genes and DNMT1 gene expression in gastric antral epithelial cells of gastric adenocarcinoma and gastritis patients. In a case-control study, 50 and 53 gastritis and gastric adenocarcinoma antral biopsies, including 23 and 21 patients with H. pylori infection, respectively, were collected from hospitals in the west of Iran. Having extracted total RNA from gastric biopsy samples, cDNA was synthesized and virulence genes of H. pylori were detected by using the PCR method. Relative real-time RT PCR was used to detect ΔΔCt fold changes of the DNMT1 gene expression in divided groups of patients based on H. pylori infection and clinical manifestations. The results showed that along with increasing patients' age, the DNMT1 gene expression will increase in gastric antral epithelial cells of gastric cancer patients (P ≤ 0.05). On the other hand, the biopsy samples with infection of H. pylori cagA, cagY, and cagE genotypes revealed a direct correlation along with increased DNMT1 gene expression. This study revealed the correlations of H. pylori cag pathogenicity island genes with increased DNMT1 gene expression.

Description of Gastritis Characteristics at the Batalaiworu Health Center, Muna Regency, Southeast Sulawesi Province, Indonesia in 2021

Gastritis is a disease that may attack all levels of society from all ages and gender. The case of gastritis becomes the biggest disease problem in Muna district. For the last 3 years, data obtained from the Batalaiworu Public Health Center showed that there has been an increase in the number of cases of gastritis in the uncertain working area of the Batalaiworu Health Center. This type of research is qualitative. The objective of study is to determine the description of patients with clinical gastritis in the working area of the Batalaiworu Health Center regarding risky eating habit, stress, smoking, coffee consumption habits and the habit of consuming non-steroidal anti-inflammatory drugs (NSAIDs). Respondents in this study were Batalaiworu sub-district people who were included in the list of gastritis patients, which were as many as 172 respondents. The results of this study reported that there is a relationship between the type of food or diet, stress, and consumption of certain drugs with the incidence of gastritis in the working area of the Batalaiworu Health Center, Muna Regency in 2021. However, the frequency of eating, coffee consumption, and smoking habits had no significant relationship with gastritis in the working area of the Batalaiworu Health Center, Muna Regency.&#x0D; Keywords: Gastritis, bad diet, stress, smoking, habit of consuming coffee, Habit of taking non-steroidal anti-inflammatory drugs (NSAIDs)

How is the Lifestyle of Gastritis Patients?

How is the Lifestyle of Gastritis Patients?

Bile reflux alters the profile of the gastric mucosa microbiota.

BackgroundBile reflux can cause inflammation, gastric mucosa atrophy, and diseases such as stomach cancer. Alkaline bile flowing back into the stomach affects the intragastric environment and can alter the gastric bacterial community. We sought to identify the characteristics of the stomach mucosal microbiota in patients with bile reflux.MethodsGastric mucosal samples were collected from 52 and 40 chronic gastritis patients with and without bile reflux, respectively. The bacterial profile was determined using 16S rRNA gene analysis.ResultsIn the absence of H. pylori infection, the richness (based on the Sobs and Chao1 indices; P <0.05) and diversity (based on Shannon indices; P <0.05) of gastric mucosa microbiota were higher in patients with bile reflux patients than in those without. There was a marked difference in the microbiota structure between patients with and without bile reflux (ANOSIM, R=0.058, P=0.011). While the genera, Comamonas, Halomonas, Bradymonas, Pseudomonas, Marinobacter, Arthrobacter, and Shewanella were enriched in patients with bile reflux, the genera, Haemophilus, Porphyromonas, and Subdoligranulum, were enriched in those without bile reflux.ConclusionOur results demonstrate that bile reflux significantly alters the composition of the gastric microbiota.