Gastric Ulcer In Male Rats Research Articles

The protective effect of benfotiamine on gastric ulcers in male rats: an experimental study.

Gastric ulcers are a common gastrointestinal disorder associated with significant morbidity and mortality. It can also increase the risk of gastric cancer. This study aimed to investigate the effect of benfotiamine on experimentally-induced gastric ulcers in male rats. In this study, 30 Wistar male rats were divided randomly into six groups: control (normal), indomethacin, omeprazole, and treatment groups, including 50, 100, and 200mg/kg of benfotiamine. Gastric ulcer was induced by indomethacin gavage. Omeprazole and different therapeutic doses of benfotiamine were administered for three days. Twenty-four hours after the last treatment, the rats were euthanized, and samples were collected.The results demonstrated that 100 and 200mg/kg of benfotiamine treatment significantly improved indomethacin-induced gastric tissue damage. Moreover, benfotiamine at 100 and 200mg/kg effectively attenuated the levels of pro-inflammatory cytokines IL-6 and TNF-α and oxidative stress markers MDA and ROS while increasing the antioxidant GSH. These findings suggest that benfotiamine's gastroprotective effects are mediated through its antioxidant and anti-inflammatory properties, which help mitigate the tissue damage and inflammatory response associated with indomethacin-induced gastric ulcers.However, further research is needed to elucidate the precise molecular mechanisms underlying these beneficial effects and to evaluate the potential therapeutic application of benfotiamine in clinical settings.

The healing effects of moderate exercise on acetic acid-induced gastric ulcer in male rats.

This study aimed to evaluate the effect of moderate exercise on the healing of acetic acid-induced gastric ulcers in male rats. Gastric ulcers include benign mucosal and submucosal lesions of the gastric wall. Exercise regulates a wide range of physiological processes. 48 male Wistar rats were randomly divided into three experimental groups (n=16 per group) as follows: control, which was left untreated after causing stomach ulcers; experimental group 1, the rats were first exercised and then received acetic acid; experimental group 2, the rats received acetic acid, and then exercised. The ulcer was caused by injecting 0.12 ml of a 60% acetic acid solution after 24 hours of not eating. The rats had a period of moderate treadmill activity either before or after the development of ulcers, lasting for a duration of 30 days. On the seventh and fourteenth days after the experiment, the rats were sacrificed, their stomach was removed, and the wound healing parameters, and wound depth were determined. Exercise before and after inducing gastric ulcers significantly decreased the depth of gastric ulcers in the experimental groups. The average number of PMN in the control group decreased in comparison to the seventh and fourteenth days following the experiment. Conversely, the number of fibroblasts, epithelialization, and new vessels increased. It seems that exercise before the appearance of ulcers has a greater effect on gastric ulcers compared to exercise after inducing gastric ulcers. Exercise can prepare the gastric mucosa for forthcoming injuries, and heal gastric ulcers. Moderate aerobic exercise has significant restorative effects on gastric ulcers caused by acetic acid and is recommended.

Anti-ulcerogenic effect of methanol fraction of Ocimum gratissimum leaves extract and honey on indomethacin-induced gastric ulcer in rats

ABSTRACT Ocimum gratissimum (Og), a medicinal plant, and honey (H), a natural sweetener usually produced by bees, have historic importance in managing several ailments. This study aims at unraveling the anti-ulcerogenic effect of methanol leaves fraction (MEF) of Og and H on indomethacin-induced gastric ulcer in male rats. Crude methanol extract was partitioned between hexane, chloroform, ethylacetate and methanol successively using vacuum liquid chromatography. The anti-ulcerogenic effect of MEF of Og (100 and 400 mg/kg), honey (2.5 g/kg) and their combination was determined using indomethacin-induced gastric ulcer model. Gastric acidity, antioxidant assays and histopathology were conducted via titrimetric, spectrophotometric, H&E stain with microscopy methods respectively. Significant difference (P < 0.05) existed in ulcer score and gastric pH in all groups except MEF pre-treated relative to control. Significant increase and decrease existed in MDA level in untreated and pre-treated groups successively compared to control. CAT activity displayed no significant difference in 400 mg/kgMEF and 400 mg/kg/2.5 g/kgH proportionate to control. GST activity showed significant elevation in pre-treated groups compared to control. Meanwhile, GPx activity increased in 400 mg/kg MEF and 400 mg/kgMEF/2.5 g/kgH relative to control. There was no significant difference in TSH status in the pre-treated groups relative to control. Mild inflammatory cellular infiltration in the sub-mucosal layers in pre-treated groups occurred. The data suggests MEF effectively prevented ulcer better than fortification with honey and honey used singly.

Histological Study of Therapeutic Effect of P.ostreatus on Gastric Ulcer in Male Rats

Mushrooms extract have wide range of medical activity. Present study goaled to investigate healing role of P.ostrateus on aspirin induced gastric ulcer. This study carried out on rats males of Rattus norvegicus, rats divided into negative control group(C), positive control group (T1), standard drug treated control group (T2), group treated with alcoholic extract of P.ostreatus (T3),group treated with polysaccharide of P.ostreatus (T4) and group treated with chitin and chitosan of p. ostreatus (T5). Histopathological examination of stomach tissue by hematoxylin and eosin, Periodic acid Schiff and Masson's trichrome of P.ostreatus extract treated group (T3) at the end of the 10 day of treatment showed epithelial cell reconstitution and gastric cell repair in gastric mucosa accompanied with marked infiltration of leucocytes and congestion in the walls of the blood vessels of the submucosal layer. At the end of 20 days, it was observed the gastric mucosa was closely no major differences compared with normal group and had better healing effect than that of omeprazole group. Histological observation of the animals treated with Polysaccharide, Chitin and Chitosan of P.ostrateus (T5 and T4) showed moderate disruption of the surface epithelium with leucocyte infiltration and thickening of muscularis mucosae at the first period. At second period there is still mild disruption and the repair is not complete. Present study detected a significant role of P.ostreatus extract in gastric ulcer healing.

Manilkara hexandra (Roxb.) Dubard Ameliorates Acetic Acid-induced Rat Gastric Ulcer

Manilkara hexandra (Roxb; Family:sapotaceae) is reported to exert preventive effect in several experimental ulcer models. However, there is no report of M. hexandra on gastric ulcer healing property. Thus, the present study was designed to evaluate the gastric ulcer healing activity of methanolic stem bark extract of M. hexandra (MH) and to derive a plausible molecular level of mechanism of action. MH was subjected to several phytochemical screening tests and standardized to quercetin by HPTLC. In the first pharmacological experiment, the standardized MH (50, 100 and 200 mg/kg) was carried out for ulcer healing activity against acetic acid (AA)-induced gastric ulcer in male rats. MH (100 and 200 mg/kg) ameliorated AA-induced rat gastric lesions. Further, MH (100 and 200 mg/kg) attenuated AA-induced changes in the levels of lipid peroxidation (LPO), reduced glutathione (GSH), oxidized glutathione (GSSG) and ratio of GSH/GSSG and activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) enzymes, and level of hame oxygenase-1 (HO-1) in stomach tissue. In the subsequent set of experiment, trigonelline (30 mg/kg; p.o.), a potent Nrf2 antagonist, significantly abrogated the gastric ulcer healing activity of MH (100 mg/kg) in AA challenged animals. Further, trigonelline attenuated the effects of MH (100 mg/kg) on the levels of LPO, GSH, GSSG and ratio of GSH/GSSG and activity of SOD, CAT, GPx and GR enzymes, and level of HO-1 in AA challenged rodents. These observations implicate the fact that MH could be a better therapeutic alternative in the management of gastric ulcer.

Royal jelly accelerates healing of acetate induced gastric ulcers in male rats.

This study examined the healing potential of royal jelly on the acetic acid induced wounds healing in male rat's gastric mucosa. Scientific reports suggest that, bee products can help in the wounds healing. 96 adult male Wistar rats were divided into in 4 groups as follows: control, omeprazole 20 mg/kg, and royal jelly 50 and 200 mg/kg). Wound was induced in stomach mucosa of each rat with 100% acetic acid. Samples groups received omeprazole or royal jelly from 1st to 14th day after acetic ulcer induction. Gastric ulcer healing and histopathological parameters were evaluated on 4, 7, 10, 15th days after ulceration. Both descriptive and statistical analyses were used. P <0.05 was considered as significant. The royal jelly administration significantly reduced the depth of lesion in comparison with the control group (p<0.05) and attuned histopathological changes in the treatment groups. The largest healing effect was demonstrated with royal jelly on 10th treatment day, at a higher concentration (200 mg/kg). These findings supported that royal jelly had effectively contributed to the wound healing, valid gastroprotective activity, and can be used for peptic ulcer therapy.

Moringa (Moringa oleifera) Leaves' Extract and Linseed (Linum usitatissimum) Oil Ameliorate Piroxicam Induced Gastric Ulcers in Male Rats

Gastric ulcer is a standout amongst the most significant gastrointestinal disorders affecting human worldwide. An extraordinary interest should be given to find natural medications to treat this disease. Plant extracts are more favored because of their wide safety margins and less or no adverse health impacts. The present study was conducted to assess the antiulcer activities of hydroethanolic extract of moringa leaves (500mg Kg BW) and linseed oil (0.3mL/ Kg BW) on piroxicam (30 mg Kg BW) induced gastric ulcers in Wister albino rats. Forty five adult male albino rats were allocated into five equal (9 each) groups designated as negative control, piroxicam, ranitidine, moringa and linseed oil groups. The results revealed that administration of piroxicam in a single oral dose induced gastric ulcers in rats, decrease in body weights (187.50±10.51g) and increase in gastric juice (1.64±0.24mL) with altered hematological, biochemical and histological findings. Meanwhile, oral administration of ranitidine, Moringa oleifera and linseed oil amelioratedthe ulcerogenic effect of piroxicam with curative ratios of 100, 97.5 and 100%, respectively. From the previous findings we concluded that hydroethanolic extract of moringa leaves and linseed oil were effective treatments of piroxicam induced gastric ulcer in rats. Antiulcerogenic effect of moringa and linseed oil may be attributed to their anti-inflammatory and antioxidant properties.

EFEK PEMBERIAN GAMBIR (Uncaria gambir roxb.) TERSTANDARISASI DAN GAMBIR YANG BERASAL DARI PAYAKUMBUH TERHADAP PERBAIKAN TUKAK LAMBUNG PADA TIKUS PUTIH JANTAN

There has been a study of the standardized gambier effect (Uncaria gambir Roxb.) And gambir derived from Payakumbuh area on the improvement of gastric ulcers in male rats induced by absolute ethanol 1 ml / 200 g BB rat. This study was an experimental study using male white rats divided into 4 groups with dosage 1 x daily for 2 days. The group consisted of negative control, positive control, 2 treatment groups (Standardized gambir and gambir Payakumbuh) with a gambir dose of 300 mg / kg BW administered orally. The method used was rat induction method using ethanol absolute where the parameters observed were recovery of peptic ulcer and pH of stomach fluid to normal. The results showed that gambier (Uncaria gambir Roxb.) Had significant effect on povary index and pH (p <0,05), where standardized gambir group was better than gambir Payakumbuh to repair peptic ulcer.

Effect of aqueous extract of aloe vera leaves on gut-brain axis response following acetic acid -induced gastric ulcer in male rats

Effect of aqueous extract of aloe vera leaves on gut-brain axis response following acetic acid -induced gastric ulcer in male rats

The effects of aqueous extract of Aloe vera leaves on the gastric acid secretion and brain and intestinal water content following acetic acid- induced gastric ulcer in male rats.

Gut-brain axis (GBA) is very important in creation and modulation of gastrointestinal problems. Aloe vera gel has gastroprotective properties. The purpose of this study was to evaluate the effect of aqueous extract of Aloe vera leaves on the gastric acid secretion and brain and intestinal water content following acetic acid gastric ulcer induction. Gastric ulcer was induced by injection of 20% acetic acid into the subserosal layer in male rats. Rats were randomly assigned into three groups: intact group, gastric ulcer group and Aloe vera group (treatment with Aloe vera following gastric ulcer induction). The acid levels and brain and intestinal water content of each sample were measured eight days after the gastric ulcer induction. Gastric acid levels were significantly decreased in Aloe vera group when compared with gastric ulcer group (p<0.05). However, there were no differences in acid output between gastric ulcer and Aloe vera groups with intact group. After Aloe vera administration, the amount of brain water content had no difference with intact and gastric ulcer groups (p<0.05). The duodenal water content in Aloe vera group was significantly reduced compared with intact group (p<0.05) but gastric ulcer group had no significant difference with intact and Aloe vera group. The administration of Aloe vera has an inhibitory effect on the gastric acid output.

Gastro-protective activity of aqueous Carica papaya seed extract on ethanol induced gastric ulcer in male rats

Gastro protective effects of aqueous Carica papaya seed extract on ethanol induced gastric ulcer were investigated in male rats. Thirty two (32) male rats weighing between 180 and 250 g were randomly divided into four groups. Group 1 served as the negative control (distilled water), groups 2 and 3 received 50mg/kg and 100mg/kg Carica papaya seed extract respectively, while group 4 received 200mg/kg cimetidine (positive control). Two weeks after the oral administration, gastric ulcer was induced in all rats with ethanol (1 ml of 80% orally). Gastric juice volume, gastric acidity, ulcer index and percentage ulcer inhibition were determined. The results showed that the extract protected the gastric mucosa against ethanol effect. C. papaya extract significantly reduced the gastric juice volume and gastric acidity (p<0.05) in dose dependent manner when compared with the control. The percentage ulcer inhibition was significantly high (p<0.05) in rats treated with the extract when compared with the control and the effect is similar to that of rats treated with cimetidine. This study shows that C. papaya seed extract may possess gastro protective effects against ethanol induced gastric ulcer in male rats.